Simlandscape: serious gaming in participatory spatial

In an attempt to improve support for contemporary spatial planning practice, Simlandscape has been developed. In this document the development of Simlandscape as ¿serious game¿ in digital form is described. In its current state, Simlandscape exists in two methodological forms; as an analogue game and as a planning support system using GIS for research and design. The game focuses on simulation of plan processes and on the resulting transformation of areas involved. Players interact with an analogue area model. The planning support system focuses on design and evaluation of plan scenarios and the data handling and presentation accompanying this process. A major challenge now is to integrate, upgrade and digitize components of the analogous game with the planning support system. Several interesting components (practical and scientific) of this project are identified and are discussed

Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking

We have previously reported that galectin-4, a tandem repeat-type galectin, regulates the raft-dependent delivery of glycoproteins to the apical brush border membrane of enterocyte-like HT-29 cells. N-Acetyllactosamine-containing glycans, known as galectin ligands, were found enriched in detergent-resistant membranes. Here, we analyzed the potential contribution of N-and/ or O-glycans in this mechanism. Structural studies were carried out on the brush border membrane-enriched fraction using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and nano-ESI-QTOF-MS/MS. The pattern of N-glycans was very heterogeneous, with the presence of high mannose- and hybrid-type glycans as well as a multitude of complex-type glycans. In contrast, the pattern of O-glycans was very simple with the presence of two major core type 1 O-glycans, sialylated and bisialylated T-antigen structures {[}Neu5Ac alpha 2-3Gal beta 1-3GalNAc-ol and Neu5Ac alpha 2-3Gal beta 1 -3(Neu5Ac alpha 2-6)GalNAc-ol]. Thus, N-glycans rather than O-glycans contain the N-acetyllactosamine recognition signals for the lipid raft-based galectin-4-dependent apical delivery. In the presence of 1-deoxymannojirimycin, a drug which inhibits the generation of hybrid-type or complex type N-glycans, the extensively O-glycosylated mucin-like MUC1 glycoprotein was not delivered to the apical brush border but accumulated inside the cells. Altogether, our data demonstrate the crucial role of complex N-glycans in the galectin-4-dependent delivery of glycoproteins to the apical brush border membrane of enterocytic HT-29 cells

Performance of a GridPix detector based on the Timepix3 chip

A GridPix readout for a TPC based on the Timepix3 chip is developed for future applications at a linear collider. The GridPix detector consists of a gaseous drift volume read out by a single Timepix3 chip with an integrated amplification grid. Its performance is studied in a test beam with 2.5 GeV electrons. The GridPix detector detects single ionization electrons with high efficiency. The Timepix3 chip allowed for high sample rates and time walk corrections. Diffusion is found to be the dominating error on the track position measurement both in the pixel plane and in the drift direction, and systematic distortions in the pixel plane are below 10 $\mu$m. Using a truncated sum, an energy loss (dE/dx) resolution of 4.1% is found for an effective track length of 1 m.Comment: To be published in Nuclear Instruments and Methods in Physics Research Section

The Oxidation Stability, Light Absorbing Power, and Component of Humic Acids from Different Origins, and Their Mutal Relations

Toxin-antitoxin modules are necessary for the mode of action of several antibiotics. One of the most studied toxin-antitoxin modules is the quorum sensing - dependent MazEF system in Escherichia coli. The quorum sensing factor in this system is called the extracellular death factor (EDF), a linear pentapeptide with the sequence NNWNN. In spite of the extensive research on the mazEF system and the involvement of the quorum sensing factor EDF, the effect of EDF itself on bacteria has not yet been studied. In this research, we determined the effect of EDF and variants on cell growth in the Gram-negative bacterium E. coli and the Gram-positive Bacillus globigii. By aligning the zwf gene (from where EDF originates) of different bacterial species, we found 27 new theoretical variants of the peptide. By evaluating growth curves and light microscopy we found that three EDF variants reduced bacterial cell size in B. globigii, but not in E. coli. The D-peptides did not affect cell size, indicating that the effect is stereospecific. Peptides wherein tryptophan was substituted by alanine also did not affect cell size, which indicates that the effect seen is mediated by an intracellular target. © 2013 Springer Science+Business Media Dordrecht

Migrant communities living in the Netherlands and their use of MT in health contexts

As part of a larger project on the use of MT in healthcare settings among migrant com- munities, this paper investigates if, when, how, and with what (potential) challenges migrants use MT based on a survey of 201 non-native speakers of Dutch currently liv- ing in the Netherlands. Three main findings stand out from our analysis. First, the data shows that most migrants use MT to under- stand health information in Dutch and com- municate with health professionals. How MT is used and received varies depending on the context and the L2 language level, as well as age, but not on the educational level. Sec- ond, some users face challenges of different kinds, including a lack of trust or perceived inaccuracies. Some of these challenges relate to comprehension, bringing us to our third point. We argue that more research is needed to understand the needs of migrants when it comes to translated expert-to-non-expert health communication. This questionnaire helped us identify several topics we hope to explore in the project's next phase.Descriptive and Comparative Linguistic

Nationwide evaluation of mutation-tailored treatment of gastrointestinal stromal tumors in daily clinical practice

Background Molecular analysis of KIT and PDGFRA is critical for tyrosine kinase inhibitor treatment selection of gastrointestinal stromal tumors (GISTs) and hence recommended by international guidelines. We performed a nationwide study into the application of predictive mutation testing in GIST patients and its impact on targeted treatment decisions in clinical practice. Methods Real-world clinical and pathology information was obtained from GIST patients with initial diagnosis in 2017-2018 through database linkage between the Netherlands Cancer Registry and the nationwide Dutch Pathology Registry. Results Predictive mutation analysis was performed in 89% of the patients with high risk or metastatic disease. Molecular testing rates were higher for patients treated in expertise centers (96%) compared to non-expertise centers (75%, P < 0.01). Imatinib therapy was applied in 81% of the patients with high risk or metastatic disease without patient's refusal or adverse characteristics, e.g., comorbidities or resistance mutations. Mutation analysis that was performed in 97% of these imatinib-treated cases, did not guarantee mutation-tailored treatment: 2% of these patients had the PDGFRA p.D842V resistance mutation and 7% initiated imatinib therapy at the normal instead of high dose despite of having a KIT exon 9 mutation. Conclusion In conclusion, nationwide real-world data show that over 81% of the eligible high risk or metastatic disease patients receive targeted therapy, which was tailored to the mutation status as recommended in guidelines in 88% of cases. Therefore, still 27% of these GIST patients misses out on mutation-tailored treatment. The reasons for suboptimal uptake of testing and treatment require further study

Evaluating model simulations of twentieth-century sea-level rise. Part II: regional sea-level changes

Twentieth-century regional sea level changes are estimated from 12 climate models from phase 5 of the Climate Model Intercomparison Project (CMIP5). The output of the CMIP5 climate model simulations was used to calculate the global and regional sea level changes associated with dynamic sea level, atmospheric loading, glacier mass changes, and ice sheet surface mass balance contributions. The contribution from groundwater depletion, reservoir storage, and dynamic ice sheet mass changes are estimated from observations as they are not simulated by climate models. All contributions are summed, including the glacial isostatic adjustment (GIA) contribution, and compared to observational estimates from 27 tide gauge records over the twentieth century (1900–2015). A general agreement is found between the simulated sea level and tide gauge records in terms of interannual to multidecadal variability over 1900–2015. But climate models tend to systematically underestimate the observed sea level trends, particularly in the first half of the twentieth century. The corrections based on attributable biases between observations and models that have been identified in Part I of this two-part paper result in an improved explanation of the spatial variability in observed sea level trends by climate models. Climate models show that the spatial variability in sea level trends observed by tide gauge records is dominated by the GIA contribution and the steric contribution over 1900–2015. Climate models also show that it is important to include all contributions to sea level changes as they cause significant local deviations; note, for example, the groundwater depletion around India, which is responsible for the low twentieth-century sea level rise in the region