6 research outputs found

    Filogenia del mono araña (genero : ateles) usando el gen mitocondrial coii

    Get PDF
    BiĂłlogo (a)Pregrad

    Cephalopod-omics: emerging fields and technologies in cephalopod biology

    Get PDF
    14 pages, 1 figure.-- This is an Open Access article distributed under the terms of the Creative Commons Attribution LicenseFew animal groups can claim the level of wonder that cephalopods instill in the minds of researchers and the general public. Much of cephalopod biology, however, remains unexplored: the largest invertebrate brain, difficult husbandry conditions, and complex (meta-)genomes, among many other things, have hindered progress in addressing key questions. However, recent technological advancements in sequencing, imaging, and genetic manipulation have opened new avenues for exploring the biology of these extraordinary animals. The cephalopod molecular biology community is thus experiencing a large influx of researchers, emerging from different fields, accelerating the pace of research in this clade. In the first post-pandemic event at the Cephalopod International Advisory Council (CIAC) conference in April 2022, over 40 participants from all over the world met and discussed key challenges and perspectives for current cephalopod molecular biology and evolution. Our particular focus was on the fields of comparative and regulatory genomics, gene manipulation, single-cell transcriptomics, metagenomics, and microbial interactions. This article is a result of this joint effort, summarizing the latest insights from these emerging fields, their bottlenecks, and potential solutions. The article highlights the interdisciplinary nature of the cephalopod-omics community and provides an emphasis on continuous consolidation of efforts and collaboration in this rapidly evolving fieldPeer reviewe

    The role of sex determination in an adaptive radiation of cichlid fishes

    No full text
    This thesis aims to expose a more detailed understanding of the intricate sex determination systems found in Lake Tanganyika cichlid species using bioinformatic tools, aiming to depict the role that sex determination has had in the explosive adaptive radiation of the LT cichlid fishes. The thesis is organized in two main parts: Part one is focused on expression data (RNA sequencing) and the characterization of sexual dimorphism, genomic differences between males and females and sex chromosome turnovers across members of the entire LT cichlid radiation and a group of LT cichlid fishes. Part two describes in more detail the sex determination system of the cichlid fish Astatotilapia burtoni in natural populations and in the developmental stages of a laboratory strain

    Time matters! Developmental shift in gene expression between the head and the trunk region of the cichlid fish Astatotilapia burtoni

    No full text
    Abstract Background Differential gene expression can be translated into differing phenotypic traits. Especially during embryogenesis, specific gene expression networks regulate the development of different body structures. Cichlid fishes, with their impressive phenotypic diversity and propensity to radiate, are an emerging model system in the genomics era. Here we set out to investigate gene expression throughout development in the well-studied cichlid fish Astatotilapia burtoni, native to Lake Tanganyika and its affluent rivers. Results Combining RNA-sequencing from different developmental time points as well as integrating adult gene expression data, we constructed a new genome annotation for A. burtoni comprising 103,253 transcripts (stemming from 52,584 genomic loci) as well as a new reference transcriptome set. We compared our transcriptome to the available reference genome, redefining transcripts and adding new annotations. We show that about half of these transcripts have coding potential. We also characterize transcripts that are not present in the genome assembly. Next, using our newly constructed comprehensive reference transcriptome, we characterized differential gene expression through time and showed that gene expression is shifted between different body parts. We constructed a gene expression network that identified connected genes responsible for particular phenotypes and made use of it to focus on genes under potential positive selection in A. burtoni, which were implicated in fin development and vision. Conclusions We provide new genomic resources for the cichlid fish Astatotilapia burtoni, which will contribute to its further establishment as a model system. Tracing gene expression through time, we identified gene networks underlying particular functions, which will help to understand the genetic basis of phenotypic diversity in cichlids
    corecore