Inferring mixed-culture growth from total biomass data in a wavelet approach

It is shown that the presence of mixed-culture growth in batch fermentation processes can be very accurately inferred from total biomass data by means of the wavelet analysis for singularity detection. This is accomplished by considering simple phenomenological models for the mixed growth and the more complicated case of mixed growth on a mixture of substrates. The main quantity provided by the wavelet analysis is the Holder exponent of the singularity that we determine for our illustrative examples. The numerical results point to the possibility that Holder exponents can be used to characterize the nature of the mixed-culture growth in batch fermentation processes with potential industrial applications. Moreover, the analysis of the same data affected by the common additive Gaussian noise still lead to the wavelet detection of the singularities although the Holder exponent is no longer a useful parameterComment: 17 pages and 10 (png) figure

Un índice de sustentabilidad para la evaluación integral de los sistemas de gestión del agua y el saneamiento

En este trabajo se presenta un índice para la evaluación de la sustentabilidad de los sistemas de gestión del agua y el saneamiento. El índice, denominado ISAS (Índice de Sustentabilidad del Agua y el Saneamiento) se basa en un marco conceptual que considera que la sustentabilidad se debería ver como una combinación de aspectos territoriales, temporales y personales. Para la construcción del ISAS se seleccionaron un conjunto de indicadores de sustentabilidad que fueron definidos en función del marco conceptual adoptado. El ISAS se utilizó para evaluar la sustentabilidad del sistema de gestión del agua y el saneamiento de la ciudad de Salta, Argentina. Los indicadores fueron calculados con información de revisiones bibliográficas, cuestionarios, visitas, talleres, datos estadísticos, y análisis de muestras de agua y aguas residuales. El método fue lo suficientemente sensible como para detectar variaciones entre diferentes aspectos del sistema estudiado. Fue también relativamente independiente de la cantidad y la calidad de la información disponible. En el caso de Salta, el valor obtenido para el ISAS fue de 51 puntos en una escala de 0 a 100 cuyo umbral de aceptabilidad se estableció en 50 puntos. El cálculo del ISAS permitió identificar una serie de aspectos del sistema que requieren mejoras y optimización. Este índice puede ser una herramienta útil para evaluar y mejorar la sustentabilidad de los sistemas de gestión del agua y el saneamiento en la región.A comprehensive sustainability index for the assessment of water and sanitation management systems (WSMS) is presented. The index was based on a conceptual framework that perceives sustainability as a combination of territorial, temporal, and personal aspects. A set of sustainability indicators was selected in agreement with this framework, and the “Water and Sanitation Sustainability Index” (WASSI) was built. The WASSI was then used to assess the sustainability of the WSMS of the city of Salta, Argentina. Indicators were calculated with information gathered by several means including literature reviews, questionnaires, visits, sampling, and participatory workshops. The index was sensitive to detect variations between different aspects of the local WSMS. It was also relatively independent of the quantity and quality of the information available. In the case of Salta, the value obtained for the index was 51 points in a scale going from 0 to 100 in which the acceptability threshold was set at 50 points. The WASSI could be a useful tool to assess and improve the sustainability of regional water and sanitation management systems.Fil: Seghezzo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: Iribarnegaray, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: Liberal, V. I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: Copa, F. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: Guerra Munizaga, M. S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: León, H. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; ArgentinaFil: Ruíz, C. Y.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigaciones en Energía no Convencional; Argentina. Universidad Nacional de Salta; Argentin

Uso actual y potencial de aguas residuales domésticas (ARD) para irrigación en la provincia de Salta, Argentina

En este trabajo se presenta un relevamiento de las principales experiencias actuales de reutilización de aguas residuales domésticas (ARD) para irrigación en la provincia de Salta, Argentina. Se identificaron tres experiencias de reúso directo y cuatro experiencias de reúso indirecto de ARD para irrigación. Las mismas se localizan en inmediaciones a los sistemas de tratamiento de líquidos cloacales. La calidad microbiológica de los efluentes indica que ningún caso cumple con las directrices propuestas por la OMS para riego irrestricto y de áreas verdes. Se estima que el potencial de reúso con ARD en la provincia asciende a unas 3500 hectáreas, considerando cultivos de referencia según la localidad. Esta estimación debe ser complementada con estudios de factibilidad para cada caso en particular. Los resultados obtenidos representan un aporte para el reconocimiento y la validación de las aguas residuales como un recurso hídrico alternativo para la región.Fil: Salas Barboza, Ariela Griselda Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Gatto D'andrea, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Garces, V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Rodriguez Alvarez, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Liberal, Viviana Isabel. Universidad Nacional de Salta; ArgentinaFil: Paoli, H.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Seghezzo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; Argentin

MAD2 haplo-insuffciency causes premature anaphase and chromosome instability in mammalian cells

The mitotic checkpoint protein hsMad2 is required to arrest cells in mitosis when chromosomes are unattached to the mitotic spindle. The presence of a single, lagging chromosome is sufficient to activate the checkpoint, producing a delay at the metaphase–anaphase transition until the last spindle attachment is made. Complete loss of the mitotic checkpoint results in embryonic lethality owing to chromosome mis-segregation in various organisms. Whether partial loss of checkpoint control leads to more subtle rates of chromosome instability compatible with cell viability remains unknown. Here we report that deletion of one MAD2 allele results in a defective mitotic checkpoint in both human cancer cells and murine primary embryonic fibroblasts. Checkpoint-defective cells show premature sister-chromatid separation in the presence of spindle inhibitors and an elevated rate of chromosome mis-segregation events in the absence of these agents. Furthermore, Mad2+/- mice develop lung tumours at high rates after long latencies, implicating defects in the mitotic checkpoint in tumorigenesis

Evaluación de la estabilidad de los lodos de las lagunas de estabilización de la zona norte de la ciudad de Salta

Se determinó la estabilidad de los lodos de las lagunas de estabilización de la zona Norte de la ciudad de Salta con la finalidad de elaborar recomendaciones para su gestión una vez clausurado el sistema de tratamiento. Se siguieron dos metodologías complementarias: el método normalizado de la Agencia de Protección Ambiental de los Estados Unidos y ensayos de estabilidad anaeróbica. Los resultados muestran que el lodo de las diferentes lagunas estudiadas se encuentra en general con un buen nivel de estabilización, lo que indicaría que el sistema operó de manera adecuada en su última etapa de funcionamiento.The stability of different samples of sludge from Waste Stabilization Ponds (WSP) was determined by means of two complementary methodologies: a standardized procedure developed by the U.S. Environmental Protection Agency and a laboratory test to measure anaerobic sludge stability. Results from this study can provide some guidelines to minimize environmental and social impacts after wastewater treatment systems such as WSP are definitely abandoned. Results obtained with both methods indicate that sludge from all WSP studied is well stabilized. This finding also suggests that the systems have been operating at low organic load in recent times.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Clinical trial evidence supporting US Food and Drug Administration approval of novel cancer therapies between 2000 and 2016

Importance: Clinical trial evidence used to support drug approval is typically the only information on benefits and harms that patients and clinicians can use for decision-making when novel cancer therapies become available. Various evaluations have raised concern about the uncertainty surrounding these data, and a systematic investigation of the available information on treatment outcomes for cancer drugs approved by the US Food and Drug Administration (FDA) is warranted. Objective: To describe the clinical trial data available on treatment outcomes at the time of FDA approval of all novel cancer drugs approved for the first time between 2000 and 2016. Design, Setting, and Participants: This comparative effectiveness study analyzed randomized clinical trials and single-arm clinical trials of novel drugs approved for the first time to treat any type of cancer. Approval packages were obtained from drugs@FDA, a publicly available database containing information on drug and biologic products approved for human use in the US. Data from January 2000 to December 2016 were included in this study. Main Outcomes and Measures: Regulatory and clinical trial characteristics were described. For randomized clinical trials, summary treatment outcomes for overall survival, progression-free survival, and tumor response across all therapies were calculated, and median absolute survival increases were estimated. Tumor types and regulatory characteristics were assessed separately. Results: Between 2000 and 2016, 92 novel cancer drugs were approved by the FDA for 100 indications based on data from 127 clinical trials. The 127 clinical trials included a median of 191 participants (interquartile range [IQR], 106-448 participants). Overall, 65 clinical trials (51.2%) were randomized, and 95 clinical trials (74.8%) were open label. Of 100 indications, 44 indications underwent accelerated approval, 42 indications were for hematological cancers, and 58 indications were for solid tumors. Novel drugs had mean hazard ratios of 0.77 (95% CI, 0.73-0.81; I2 = 46%) for overall survival and 0.52 (95% CI, 0.47-0.57; I2 = 88%) for progression-free survival. The median tumor response, expressed as relative risk, was 2.37 (95% CI, 2.00-2.80; I2 = 91%). The median absolute survival benefit was 2.40 months (IQR, 1.25-3.89 months). Conclusions and Relevance: In this study, data available at the time of FDA drug approval indicated that novel cancer therapies were associated with substantial tumor responses but with prolonging median overall survival by only 2.40 months. Approval data from 17 years of clinical trials suggested that patients and clinicians typically had limited information available regarding the benefits of novel cancer treatments at market entry

Reúso de aguas residuales domésticas en la actividad agropecuaria: el caso de Cafayate, Salta

En este trabajo se presenta una experiencia de más de 50 años en reutilización de aguas residuales domésticas para riego agrícola en una zona árida de la provincia de Salta, Argentina. Se realiza una descripción general del estudio de caso, una evaluación preliminar de la aptitud sanitaria, agronómica y ambiental del efluente tratado para riego y una apreciación del productor sobre las ventajas y desventajas de la experiencia. Los resultados indican que el efluente tratado no tendría restricciones para riego en cuanto a salinidad, permeabilidad y toxicidad. La calidad sanitaria del efluente tratado es baja, detectándose elevados niveles de bacterias coliformes fecales. El sistema de tratamiento no remueve los microorganismos debido a su escaso tiempo de retención hidráulica. Se recomienda continuar con el riego de cultivos restringidos y aplicar medidas precautorias en la manipulación del efluente.This article describes a 50-year long experience of irrigation with domestic wastewater in an arid región of the province of Salta, Argentina. We provide a general description of the case study, a preliminary assessment of the sanitary, agricultural, and environmental quality of the wastewater, and an overview of the perception of the producer on this activity. Results show that the wastewater can be safely used for irrigation in terms of its salinity and toxicity. Sanitary quality is low, with high levels of fecal coliforms. The wastewater treatment system does not efficiently remove pathogens due to its short hydraulic retention time. Application of this wastewater must be avoided for vegetables that are consumed raw, and some precautionary measures should be taken to reduce manipulation risks.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Maintaining order in the drug game: Applying harm reduction principles to drug detective work

Drug war rhetoric is losing favour in the political arena due to its increasingly obvious failures and there is a growing consensus amongst governing elites that something ought to change. Against this backdrop, the concept of ‘harm reduction’ has moved to the foreground of the research agenda and some policing agencies appear to be reconfiguring their enforcement interventions to focus on managing drug markets in a way that minimises the various associated harms. This article draws on the findings of an ethnographic study of specialist detective units in two English police services to examine how recent developments in drug policy discourse have been received and implemented at an operational level. Although there have been some positive advancements, it argues that changes to the prohibition regime are largely superficial and strong cultural resistance remains. The discussion considers how harm reduction principles might be better applied to the policing of drug markets

Trece tratamientos de la insuficiencia renal aguda secundaria a mieloma múltiple con filtros de high cut off

Introduction Multiple myeloma (MM) is a haematological tumour that is characterised by uncontrolled proliferation of plasma cells and a significant volume of serum free light chains (sFLCs), which can cause acute renal failure due to intratubular precipitation, resulting in cast nephropathy. Acute renal failure is a complication that can arise in more than 20% of patients with multiple myeloma, half of which will require dialysis. Methods We report our experience with 13 patients who were treated with dialysis using high cut off filters (HCO) between July 2011 and February 2015. A total of 6 consecutive 6-hour sessions were performed using a 2.1 m2 HCO filter (Theralite® by Gambro®). Afterwards, further 6-hour sessions were continued on alternate days. Results A total of 151 sessions were conducted, with an average of 11.6 sessions per patient (range 6-27). The treatment proved to be effective in removing both kappa and lambda sFLCs, resulting in a 93.7% fall in sFLCs by the end of treatment. The average reduction was 57.7% per dialysis session. 10 out of the 13 cases recovered sufficient renal function to become independent of dialysis. There were no major changes in albumin levels using an infusion protocol of 2 50-mL vials of 20% albumin at the end of the dialysis session. Conclusions Combination treatment with chemotherapy and long dialysis with HCO filters was effective in reducing the sFLC levels and recovering sufficient renal function in 77% of cases. With HCO filters, significant cost savings are achieved, contrary to what was previously believed

Uso actual y potencial de aguas residuales domésticas (ARD) para irrigación en la provincia de Salta, Argentina

En este trabajo se presenta un relevamiento de las principales experiencias actuales de reutilización de aguas residuales domésticas (ARD) para irrigación en la provincia de Salta, Argentina. Se identificaron tres experiencias de reúso directo y cuatro experiencias de reúso indirecto de ARD para irrigación. Las mismas se localizan en inmediaciones a los sistemas de tratamiento de líquidos cloacales. La calidad microbiológica de los efluentes indica que ningún caso cumple con las directrices propuestas por la OMS para riego irrestricto y de áreas verdes. Se estima que el potencial de reúso con ARD en la provincia asciende a unas 3500 hectáreas, considerando cultivos de referencia según la localidad. Esta estimación debe ser complementada con estudios de factibilidad para cada caso en particular. Los resultados obtenidos representan un aporte para el reconocimiento y la validación de las aguas residuales como un recurso hídrico alternativo para la región.This article describes a survey of the main cases of domestic wastewater reuse for irrigation in the province of Salta (Argentina). We identified 3 cases of direct reuse and 4 cases of indirect reuse to irrigate crops and green areas. Reuse practices are generally located close to sewage treatment plants, mainly waste stabilization ponds managed by the state-owned water utility. We made some laboratory analysis to assess the microbiological characteristics of the wastewater. None of the cases studied comply with WHO’s guidelines for unrestricted irrigation. We estimated that the area that could be irrigated with treated domestic wastewater in the province can be up to 3500 hectares. This estimation need to be validated with feasibility studies on a case-by-case basis. Results obtained in this work are a contribution to the recognition and validation of the use of domestic wastewater as an alternative water resource in the region.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES