Enteroparasitological profile of patients assisted in the Lauro Wanderley University Hospital (HULW)

Infections with intestinal parasites represent a worsening public health, given the large number of individuals affected and various organic changes that can be caused. The objective was to conduct a survey of individuals affected by these parasites assisted by the Clinical Laboratory of the HULW from January 2010 to January 2011, as well as characterizing data intrinsic to individuals. Data were obtained through a statistical analysis of 7844 reports of fecal examinations. Among the results was shown that 32.5 % of samples were parasitized, Ascaris lumbricoides being the most frequent in these reports, accounting for 36 %. Female patients were the most affected, comprising 62 % of positive cases. It was detected the existence of multiple parasitism in 30.7 %. Therefore, the high frequency of intestinal parasites detected is a reality that needs to be minimized in the population assisted by the HULW’s Clinical Laboratory.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Isolation, absolute configuration and cytotoxic activities of alkaloids from Hippeastrum goianum (Ravenna) Meerow (Amaryllidaceae)

The phytochemical study of Hippeastrum goianum led to the identification of 13 compounds by means of gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). Compounds 7-demethoxy-9-O-methylhostasine (1) and 7-deoxi-trans-dihydronarciclasine (2) had their absolute configurations determined by vibrational circular dichroism (VCD). This is the first time that compound 1 is described in the Amaryllidaceae family. The cytotoxicity of all isolated compounds was tested against colorectal carcinoma (HCT 116), breast carcinoma (MCF-7), and non-tumor human retinal pigment epithelium (RPE) cell lines. The half-maximum inhibitory concentration (IC50) of compound 2 against each cell line was equivalent to the positive control (doxorubicin), indicating a considerable cytotoxic activity. Keywords: narciclasine; galasine; cytotoxic activity; absolute configuration; vibrational circular dichrois

Evaluation of antitumour and antiinflammatory effects and acute toxicity of extracts obtained from Streptomyces spp. isolated from m Soils of Paraiba (Brazil)

Bioactive metabolites produced by Streptomyces spp. commonly exhibit a variety of pharmacological properties such as antibiotic, antitumor, enzymatic and anti-helminthic. The study evaluated the possible antitumor and anti-inflammatory effects and the degree of toxicity of extracts isolated from Streptomyces in experimental models with animals. The extracts Sp-1 and Sp-3 did not have anti-inflammatory effect. In the Sarcoma 180 model the effects of Sp-1 and Sp-3 were significant with decreased average weights of tumors at 10 mg/kg, and reduction of up to 73 % of initial weight of the implanted tumor. For tumors of Ehrlich Carcinoma, the doses showed no significant effect on the average weight of tumors. Stimulant effects, such as exophthalmia, agitation, escape reaction, irritability, tremors and dermatitis were observed after 1 h of administration, depressive reactions were also observed, such as prostration and decreased respiratory rate, and no deaths were highlighted.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Subclinical pulmonary hypertension in childhood systemic lupus erythematosus associated with minor disease manifestations

The aim of this study was to evaluate pulmonary hypertension (PH) in 852 childhood-onset systemic lupus erythematosus (cSLE) patients. This was a large multicenter study conducted in 10 Pediatric Rheumatology Services of So Paulo state, Brazil. PH was defined as systolic pulmonary artery pressure > 35 mmHg and/or measurement of the mean pulmonary artery pressure > 25 mmHg and/or diastolic pressure > 15 mmHg by transthoracic echocardiogram. Demographic data, clinical manifestations, disease activity score (SLEDAI-2K), disease damage score (SLICC/ACR-DI) and treatments were also evaluated. Statistical analysis was performed using Bonferroni correction (p 0.002). Normal transthoracic echocardiography was evidenced in 9/17 (53%), with median cSLE duration of 17.5 months (1-40) after PH standard treatment. PH was a rare manifestation of cSLE occurring in the first two years of disease. The majority of patients were asymptomatic with mild lupus manifestations. The underlying mechanism seemed not to be related to pulmonary thromboembolism and/or antiphospholipid syndrome382234239CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ301805/2013-0; 303752/2015-7; 301479/2015-1; 305068/2014-8; 303422/2015-

Subclinical Pulmonary Hypertension in Childhood Systemic Lupus Erythematosus Associated with Minor Disease Manifestations

The aim of this study was to evaluate pulmonary hypertension (PH) in 852 childhood-onset systemic lupus erythematosus (cSLE) patients. This was a large multicenter study conducted in 10 Pediatric Rheumatology Services of So Paulo state, Brazil. PH was defined as systolic pulmonary artery pressure > 35 mmHg and/or measurement of the mean pulmonary artery pressure > 25 mmHg and/or diastolic pressure > 15 mmHg by transthoracic echocardiogram. Demographic data, clinical manifestations, disease activity score (SLEDAI-2K), disease damage score (SLICC/ACR-DI) and treatments were also evaluated. Statistical analysis was performed using Bonferroni correction (p 0.002). Normal transthoracic echocardiography was evidenced in 9/17 (53%), with median cSLE duration of 17.5 months (1-40) after PH standard treatment. PH was a rare manifestation of cSLE occurring in the first two years of disease. The majority of patients were asymptomatic with mild lupus manifestations. The underlying mechanism seemed not to be related to pulmonary thromboembolism and/or antiphospholipid syndrome.Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Federico FoundationNucleo de Apoio a Pesquisa "Saude da Crianc, a e do Adolescente'' da USP (NAP-CriAd)Univ Sao Paulo, Fac Med, Childrens Inst, Pediat Rheumatol Unit, Sao Paulo, BrazilUniv Sao Paulo, Fac Med, Div Rheumatol, Ave Dr Arnaldo 455,3 Andar, BR-05403010 Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Med, Childrens Inst, Pediat Echocardiog Unit, Sao Paulo, BrazilUniv Fed Sao Paulo, Pediat Rheumatol Unit, Sao Paulo, BrazilSao Paulo State Univ UNESP, Fac Med Botucatu, Sao Paulo, BrazilUniv Estadual Campinas, Campinas, SP, BrazilIrmandade Santa Casa Misericordia Sao Paulo, Sao Paulo, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Sao Paulo, BrazilHosp Menino Jesus, Sao Paulo, BrazilUniv Fed Sao Paulo, Pediat Rheumatol Unit, Sao Paulo, BrazilCNPq: 301805/2013-0CNPq: 303752/2015-7CNPq: 301479/2015-1CNPq: 305068/2014-8CNPq: 303422/2015-7Web of Scienc