2,3-Bis(2-chloro­benz­yl)naphthalene-1,4-dione

The title disubstituted naphthalene-1,4-dione, C24H16Cl2O2, has the two chloro­benzyl substituents related by a non-crystallographic twofold rotation axis, generating a chiral conformation; both enantiomers are present. The two chlorobenzene rings are nearly perpendicular to the fused ring system, making angles of 88.8 (1) and 77.5 (1)° with it

Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond between the residues H44 and L100. The stability of scFv (H4) increased from a GdnHCl50 of 2.4 M to 4.2 M after addition of the H44-L100 disulfide bond. Size exclusion chromatography revealed that the scFv (H44-L100) mutant existed primarily as a monomer, and no aggregates were detected. An affinity assay indicated that scFv (H4) and the scFv (H44-L100) mutant had similar IC50 values and affinity to AFB1. Our results indicate that interdomain disulfide bonds could stabilize scFv without affecting affinity

Pseudo-cyclic Face-to-face Rigid Structure Caused by the Intramolecular Ion Pair Effect

molecule

Rapid Commun

Combined treatment of hepatocellular carcinoma with partial splenic embolization and transcatheter hepatic arterial chemoembolizatio

The reaction of tanshinones with diamines

2105-2111The reactions of cryptotanshinone and tanshinone IIA with diamines, including putrescine and cadaverine are carried out. Eleven new compounds, containing tetrahydrophenanthroimidazole and tetrahydrophenanthrooxazole rings, are synthesized and the possible reaction mechanism is proposed

Asymmetric Hydrogenation of 3,5-Bistrifluoromethyl Acetophenone in Pilot Scale with Industrially Viable Ru/Diphosphine–Benzimidazole Complexes

A novel efficient asymmetric hydrogenation (AH) process was developed for the preparation of (<i>R</i>)-1-(3,5-bis­(trifluoromethyl)­phenyl)­ethanol (<b>3</b>), using a catalyst Ru/(4<i>R</i>,5<i>R</i>)-(+)-4,5-bis­(diphenylphosphinomethyl)-2,2-dimethyl-1,3-dioxoane-(<i>R</i>,<i>R</i>-Diop)-2<i>R</i>-(α-methylmethanamine)-4,7-dimethyl-1<i>H</i>-benzo­[<i>d</i>]­imidazole (<i>R</i>-D-Me-BIMAH) in toluene in the presence of potassium <i>t</i>-butoxide. Various hydrogenation parameters, such as ligand, solvent, and substrate-to-catalyst (S/C) ratio, were investigated. The hydrogenation was carried out for four times on a 5 kg scale at 30 atm and 25 °C with S/C of 20 000 with an enantiomeric excess of >89%