Analysis of HER2 Gene Amplification and Certain Prognostic Factors in Breast Cancer

Objective: The HER2 gene amplification and certain prognostic factors in breast cancer were analyzed. Method: The gene amplification and protein expression of human epidermal growth factor receptor in 100 breast cancer tissues detected by FISH and IHC detection method in the hospital from January 2020 to December 2021 were analyzed. To analyze some breast cancer prognostic factors. Result: 0 is 8 cases of HER-2 protein breast cancer, (1+) is 11 cases, (2+) is 49 cases, (3+) is 32 cases. The HER2 gene was amplified in 49 cases, of which 23 cases showed red signals in clusters, and 26 cases showed red signals in dots. 51 cases of HER-2 gene were not amplified. There are differences in the detection results of FISH and IHC detection methods (P＞0.05). ER, PR and polysomy of chromosome 17 are prognostic factors associated with HER2 gene amplification in certain breast cancers. (P＜0.05) Conclusion: To analyze the HER2 gene amplification in breast cancer and targeted select FISH and IHC detection methods can improve the therapeutic effect and prognostic factor, which deserves clinical attention

硫酸氢氯吡格雷联合西洛他唑治疗糖尿病四肢并发症的临床疗效观察

Objective: To observe efficacy and safety of domestic clopidogrel cilostazol in treatment of diabetic limbs complications. Methods: 120 cases of diabetic patients with limb complications were randomly selected, including 60 cases of lower extremity arterial disease (PAD) and 60 patients with diabetic peripheral neuropathy (DPN) patients. According to the random number list, they were randomly divided into treatment group of 60 patients, DPN and PAD were given mecobalamin 0. 5mg / times , lipoic acid 500mg / d, alprostadil 10ug / d, DPN patients while giving epalrestat 50mg / times. The control group while giving cilostazol 0.1g, 2 times / d, plus clopidogrel treatment groups 150mg / d in the control group, based on continuous treatment for 8 weeks. Clinical evaluation of the two groups was made and conduction velocity of MNCV and SNCV was compared. Results: Analysis of DPN subgroup showed that the treatment group was efficient (73.4% vs 46.7%, P = 0.035), total efficiency (93.3% vs 73.3%, P = 0.038) were significantly higher, PAD subgroup analysis showed the efficiency of the treatment group was (70% vs 43.3%, P = 0.035), total efficiency (93.3% vs 73.3%, P = 0.038) were significantly higher. MNCV CKS nerve (54.3 ± 4.8 vs 50.5 ± 5.1) and the peroneal nerve (34.7 ± 3.2 vs 30.9 ± 3.4) conduction velocity improved more significantly. SNCV CKS nerve (43.4 ± 4.9 vs 38.2 ± 3.8) and the peroneal nerve (35.1 ± 5.6 vs 32.9 ± 5.4) conduction velocity, the treatment group improved significantly than the control group. PAD subgroup analysis, ABI degree of improvement (0.9 ± 0.3 vs 0.8 ± 0.3), dorsal blood flow (35.2±3.6 vs31.5±4.2), popliteal artery blood flow (64.2±5.6 vs 58.4±7.4) were better than the control group. Compared with clopidogrel, cilostazol did not increase in adverse events during treatment process. Conclusion: Clopidogrel cilostazol treatment of diabetic limb complications becomes safe and effective treatment of DPN and PAD, which can be used as a combination therapy.目的  观察国产氯吡格雷联合西洛他唑治疗糖尿病四肢并发症的有效性及安全性。方法  随机入选120例糖尿病四肢并发症患者，其中糖尿病周围神经病变（DPN）患者60例，下肢动脉病变（PAD）60例。按照随机数列表，随机分为治疗组60例，DPN及PAD均给予甲钴胺0. 5mg /次，硫辛酸500mg/d，前列地尔10ug/d，DPN患者同时给予依帕司他50mg /次。对照组同时给予西洛他唑0.1g，2次/d，治疗组在对照组基础上加上氯吡格雷首次600mg，后150mg/d连续7d，后改为75mg/d连续8周。评价两组的临床疗效及MNCV和SNCV传导速度。结果  DPN亚组分析显示，治疗组显效率（73.4% vs 46.7%, P=0.035）、总有效率（93.3% vs 73.3%，P=0.038）显著高于对照组，PAD亚组分析显示，治疗组显效率（70% vs 43.3%, P=0.035）、总有效率（93.3% vs 73.3%，P=0.038）显著高于对照组。MNCV中正中神经（54.3±4.8 vs 50.5±5.1）及腓总神经（34.7±3.2 vs 30.9±3.4）传导速度改善更为明显。SNCV中正中神经（43.4±4.9 vs 38.2±3.8）及腓总神经(35.1±5.6 vs 32.9±5.4)传导速度，治疗组改善亦较对照组更明显。PAD亚组分析显示，ABI改善程度（0.9±0.3 vs 0.8±0.3）、足背动脉血流速度（35.2±3.6 vs31.5±4.2），腘动脉血流量（64.2±5.6 vs 58.4±7.4）改善均优于对照组。治疗过程中氯吡格雷相比于西洛他唑并不增加不良事件。结论  氯吡格雷联合西洛他唑治疗糖尿病四肢并发症变安全、有效，可作为治疗DPN及PAD的联合治疗方案

Modelling bus delay at bus stop

A bus may be blocked from entering and exiting a stop by other buses and traffic lights. The objective of this paper is to model each type of delay under these phenomena and the overall delay a bus experiences at a stop. Occupy-based delay, transfer block-based delay and block-based delay are defined and modelled. Bus delay at stop is just the sum of these three types of delay. Bus arrival rate, bus service rate, berth number and traffic lights are taken into consideration when modelling delay. Occupy-based delay is modelled with mean waiting time in Queueing theory. Transfer block-based delay and block-based delay are modelled based on standard deviation of waiting time and the probabilities of their occurrences. Two stops in Vancouver, Canada are selected for parameter estimation and model validation. The unknown parameter is estimated as 0.4230 using Ordinary Least Squares (OLS), which indicates that 42.3% of waiting time variation can be attributed to buses being blocked by the buses in front and red light for the selected stops. Model validation shows the average accuracy rate of the proposed model is 75.07% for the selected stops. Bus delay at stop evidently increases when arrival rate is more than 85 buses per hour for the given service time (50 s), ratio of red time to cycle length (0.65) and berth number (2). We also figure out that bus delay at stop evidently increases when service time is more than 60 s for the given arrival rate (54 buses per hour), ratio of red time to cycle length (0.65) and berth number (2). The proposed model can provide a tool for bus stop design and offer the foundation for service quality evaluation of transit. First published online 28 January 201

The Oxidation of the HiSiMo Cast Irons Alloyed with Cr/Al at 800 °C

To improve the oxidation resistance of cast iron containing Si and Mo (HiSiMo), 1wt.% Cr and 3wt.% Al were added into the base alloy to improve high temperature oxidation resistance for exhaust manifold applications. Oxidation tests were performed in both dry and wet air (air + 10% H2O) at 800 °C for 1, 10, 24, and 50 h. The mass change and oxide thickness were measured to evaluate the oxidation resistance. Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDS) and transmission electron microscopy (TEM) were employed to characterize the oxidized samples. The results showed that the additions of Al or Cr selectively oxidized to form a more oxidation resistant surface oxide layer, which reduced the mass gain during these experiments

Robust nanogenerators based on graft copolymers via control of dielectrics for remarkable output power enhancement

A robust nanogenerator based on poly(tert-butyl acrylate) (PtBA)-grafted polyvinylidene difluoride (PVDF) copolymers via dielectric constant control through an atom-transfer radical polymerization technique, which can markedly increase the output power, is demonstrated. The copolymer is mainly composed of ?? phases with enhanced dipole moments due to the ??-bonding and polar characteristics of the ester functional groups in the PtBA, resulting in the increase of dielectric constant values by approximately twice, supported by Kelvin probe force microscopy measurements. This increase in the dielectric constant significantly increased the density of the charges that can be accumulated on the copolymer during physical contact. The nanogenerator generates output signals of 105 V and 25 ??A/cm2, a 20-fold enhancement in output power, compared to pristine PVDF-based nanogenerator after tuning the surface potential using a poling method. The markedly enhanced output performance is quite stable and reliable in harsh mechanical environments due to the high flexibility of the films. On the basis of these results, a much faster charging characteristic is demonstrated in this study

A Large-scale Benchmark for Log Parsing

Log data is pivotal in activities like anomaly detection and failure diagnosis in the automated maintenance of software systems. Due to their unstructured format, log parsing is often required to transform them into a structured format for automated analysis. A variety of log parsers exist, making it vital to benchmark these tools to comprehend their features and performance. However, existing datasets for log parsing are limited in terms of scale and representativeness, posing challenges for studies that aim to evaluate or develop log parsers. This problem becomes more pronounced when these parsers are evaluated for production use. To address these issues, we introduce a new collection of large-scale annotated log datasets, named LogPub, which more accurately mirrors log data observed in real-world software systems. LogPub comprises 14 datasets, each averaging 3.6 million log lines. Utilizing LogPub, we re-evaluate 15 log parsers in a more rigorous and practical setting. We also propose a new evaluation metric to lessen the sensitivity of current metrics to imbalanced data distribution. Furthermore, we are the first to scrutinize the detailed performance of log parsers on logs that represent rare system events and offer comprehensive information for system troubleshooting. Parsing such logs accurately is vital yet challenging. We believe that our work could shed light on the design and evaluation of log parsers in more realistic settings, thereby facilitating their implementation in production systems