Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis.

Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family. In apple (Malus domestica), antisense suppression of aldose-6-phosphate reductase, the key enzyme for sorbitol synthesis, significantly decreased the sorbitol concentration but increased the sucrose concentration in leaves, leading to a lower sorbitol but a higher sucrose supply to fruit in these plants. In response to this altered carbon supply, the transgenic fruit had lower concentration of sorbitol and much higher concentration of glucose but similar levels of fructose, sucrose, and starch throughout fruit development relative to the untransformed control. Activities of sorbitol dehydrogenase, fructokinase, and sucrose phosphate synthase were lower, whereas activities of neutral invertase, sucrose synthase, and hexokinase were higher in the transgenic fruit during fruit development. Transcript levels of MdSOT1, MdSDHs, MdFK2, and MdSPS3/6 were downregulated, whereas transcript levels of MdSUC1/4, MdSUSY1-3, MdNIV1/3, MdHKs, and MdTMT1 were upregulated in the transgenic fruit. These findings suggest that the Sucrose cycle and the sugar transport system are very effective in maintaining the level of fructose and provide insights into the roles of sorbitol and sucrose in regulating sugar metabolism and accumulation in sorbitol-synthesizing species

Relationships between structure and antioxidant capacity and activity of glycosylated flavonols

The antioxidant capacity (AC) and antioxidant activity (AA) of three flavonols (FLV), aglycones and their glycosylated derivatives were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays in various solvents. Findings confirmed that the glycosylation at the 3-position (3-glycosylation) always decreased the AC under most conditions due to substitution of the 3-position hydroxyl group and glycoside disruption in the molecular planarity. The 7-glycosylated derivatives did not have the above effects, thus generally exhibited ACs similar to their aglycones. Glycosylation decreased the AA of kaempferol and isorhamnetin for both assays in methanol, 3-glycosylation inhibited quercetin AA in the ABTS assay. In the DPPH assay, the AA of 3-glycosylated quercetin was significantly higher than quercetin. Using LC–MS/MS analysis, we found that quercetin and quercetin-7-glucoside underwent dimerization during the antioxidant reaction, potentially leading to a decline in AAs. However, 3-glycoside substitution may have hindered dimer formation, thereby allowing the FLVs to retain strong free radical scavenging abilities.National Key Research and Development Program of China | Ref. 2019YFC160670

Frequently asked questions about chlorophyll fluorescence, the sequel

[EN] Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122: 121-158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additionalChl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of F-V/F-M values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge fromdifferent Chl a fluorescence analysis domains, yielding in several cases new insights.Kalaji, H.; Schansker, G.; Brestic, M.; Bussotti, F.; Calatayud, A.; Ferroni, L.; Goltsev, V.... (2017). Frequently asked questions about chlorophyll fluorescence, the sequel. 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Research on Fatigue Strength for Weld Structure Details of Deck with U-rib and Diaphragm in Orthotropic Steel Bridge Deck

The orthotropic steel bridge deck weld structure would easily cause fatigue cracking under the repeated action of vehicle load. This paper took the steel box girder in a bridge as a research object, researched the mechanical properties of the steel plate and the microstructure of the welded joint, then designed the fatigue specimens of the deck plate and did the fatigue test. The Δσ-N curves and stress amplitudes of the weld details of the deck plate with U-rib and diaphragm under different probabilities of survival were obtained. After extended the Δσ-N curves to the long life range, the fatigue damage calculation equation of the detail was proposed, and the cut-off limit under the 50% and 97.7% probability of survival were 81.50 MPa and 53.11 MPa, respectively. Based on the actual vehicle load spectrum and simplified finite element model of the steel box girder section, the stress amplitude of the details of the weld joint was calculated. The calculation result shows that the maximum stress amplitude of the concerned point was 38.29 MPa, less than the cut-off limit. It means that the fatigue strength of the details of the weld joint meet the requirement of the fatigue design

Fatigue Property and Improvement of a Rounded Welding Region between the Diaphragm Plate and Closed Rib of an Orthotropic Steel Bridge Deck

By means of finite element modeling (FEM) and fatigue experiments, we study the fatigue performance of the rounded welding region between the diaphragm plate and closed rib of orthotropic steel bridge deck in this work. A local sub-model of the rounded welding region from the orthotropic steel bridge deck was developed to analyze the stress distributions. Based on the analysis results we designed the fatigue specimen for the fatigue test of this detailed structure. The fatigue experimental results revealed that the crack initiates from the weld toe of the rounded welding region and the stress concentration at the rounded welding region is the main mechanism of fatigue crack initiation. In addition, we propose three improvements to reduce the stress concentration of the rounded welding region, and the local structure optimization scheme of the diaphragm–rib weld can effectively improve the fatigue resistance of the detailed weld structure

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Sunlight radiation is a main environmental factor which affects anthocyanin synthesis. To clarify the regulatory mechanism of sunlight on the synthesis of anthocyanin in apple peel, bagged apples were exposed to diverse intensities of sunlight through different shading treatments. Under an increased solar ultraviolet-B (UV-B) light intensity, the concentration of anthocyanin in apple peels was consistent with the Michaelis–Menten equation. Under lower sunlight intensities, diphenyleneiodonium chloride (DPI, an inhibitor of plasma membrane NAD(P)H oxidase) treatment increased both the concentration of cyanidin-3-glycoside and the activity of dihydroflavonol 4-reductase (DFR). However, under higher sunlight intensities, DPI treatment decreased the concentrations of cyanidin-3-glycoside and quercetin-3-glycoside, as well as the activities of DFR and UDP-glycose: flavonoid 3-O-glycosyltransferase (UFGT). These results indicate that, under low sunlight intensity, anthocyanin synthesis in apple peel was limited by the supply of the substrate cyanidin, which was regulated by the DFR activity. Nevertheless, after exposure to high sunlight intensity, the anthocyanin produced in the apple peel was dependent on UFGT activity

Fatigue Life of a Comb Plate Expansion Joint

Comb-plate expansion joints are widely used in bridge construction, and their failures are mainly static strength and fatigue. This paper used a new type of comb-plate expansion joint as the research object. Firstly, the finite element models (FEM) of the comb-plate expansion joint with minimum and maximum openings were established by Ansys software. Then, the equivalent stress, vertical deformation, and shear stress of the expansion joint under these two working conditions were checked with code. The results showed that the static strength of the expansion joints met the code requirements under both working conditions. Secondly, to investigate the service span of the comb-plate expansion joint, the fatigue life of the expansion joint was predicted using nCode DesignLife software, and the results showed that the minimum fatigue life of the expansion joint was 2.012 × 106 times, which is higher than the 2 × 106 times specified in the code. Finally, a fatigue test of 2 million times was carried out on the full-size expansion joint. Failure modes such as deformation, fracture, or breakage hardly appeared after the fatigue test, demonstrating the reliability of this new type of comb-plate expansion joint

Photoinhibition-like damage to the photosynthetic apparatus in plant leaves induced by submergence treatment in the dark.

Submergence is a common type of environmental stress for plants. It hampers survival and decreases crop yield, mainly by inhibiting plant photosynthesis. The inhibition of photosynthesis and photochemical efficiency by submergence is primarily due to leaf senescence and excess excitation energy, caused by signals from hypoxic roots and inhibition of gas exchange, respectively. However, the influence of mere leaf-submergence on the photosynthetic apparatus is currently unknown. Therefore, we studied the photosynthetic apparatus in detached leaves from four plant species under dark-submergence treatment (DST), without influence from roots and light. Results showed that the donor and acceptor sides, the reaction center of photosystem II (PSII) and photosystem I (PSI) in leaves were significantly damaged after 36 h of DST. This is a photoinhibition-like phenomenon similar to the photoinhibition induced by high light, as further indicated by the degradation of PsaA and D1, the core proteins of PSI and PSII. In contrast to previous research, the chlorophyll content remained unchanged and the H2O2 concentration did not increase in the leaves, implying that the damage to the photosynthetic apparatus was not caused by senescence or over-accumulation of reactive oxygen species (ROS). DST-induced damage to the photosynthetic apparatus was aggravated by increasing treatment temperature. This type of damage also occurred in the anaerobic environment (N2) without water, and could be eliminated or restored by supplying air to the water during or after DST. Our results demonstrate that DST-induced damage was caused by the hypoxic environment. The mechanism by which DST induces the photoinhibition-like damage is discussed below

Dynamic Modeling of a Hydraulic Excavator Stick by Introducing Multi-Case Synthesized Load Spectrum for Bench Fatigue Test

A multi-case load spectrum compiling method is proposed in this study for dynamic modeling of a hydraulic excavator stick to simplify and accelerate the fatigue bench test. This new method includes a simplified criterion of small-load-omitting threshold based on the principle of invariable fatigue damage, an extreme value inference criterion based on the overflow characteristics of the hydraulic system, and a synthetic extrapolation method under various working conditions. Firstly, a one-dimensional spectrum of a medium-sized excavator stick was compiled. Then, the program load spectrum for the bench fatigue test was obtained by modifying the one-dimensional spectrum based on the damage consistency criterion and the damage equivalent principle. Lastly, the fatigue tests were conducted using the program load spectrum, as well as using the random spectrum. The comparison results demonstrate that the damage location and fatigue life distribution of the stick using these two spectra are generally consistent, with a relative error smaller than 8.8%; however, the proposed program load spectrum can accelerate the test process with less time consuming than that of the random spectrum. As a result, the multi-case load spectrum is feasible and reliable for dynamic modeling of the hydraulic excavator stick in practice