Auricle shaping using 3D printing and autologous diced cartilage.

ObjectiveTo reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP).MethodsMaterialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.ResultsThe 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.ConclusionAuricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.Levels of evidenceNA. Laryngoscope, 129:2467-2474, 2019

Design, Implementation and Modeling of Flooding Disaster-Oriented USV

Although there exist some unmanned surface platforms, and parts of them have been applied in flooding disaster relief, the autonomy of these platforms is still so weak that most of them can only work under the control of operators. The primary reason is the difficulty of obtaining a dynamical model that is sufficient rich for model-based control and sufficient simple for model parameters identification. This makes them difficult to be used to achieve some high-performance autonomous control, such as robust control with respect to disturbances and unknown dynamics and trajectory tracking control in complicated and dynamical surroundings. In this chapter, a flooding disaster-oriented unmanned surface vehicle (USV) designed and implemented by Shenyang Institute of Automation, Chinese Academy of Sciences (SIA, CAS) is introduced first, including the hardware and software structures. Then, we propose a quasi-linear parameter varying (qLPV) model to approach the dynamics of the USV system. We first apply this to solve a structured modeling problem and then introduce model error to solve an unstructured modeling problem. Subsequently, the qLPV model identification results are analyzed and the superiority compared to two linear models is demonstrated. At last, extensive application experiments, including rescuing rope throwing using an automatic pneumatic and water sampling in a 2.5 m radius circle, are described in detail to show the performance of course keeping control and GPS point tracking control based on the proposed model

Experimental Investigation on the Early Stage Spray Characteristics with Biodiesel and Diesel

[EN] The early stage spray characteristics have a great impact on the secondary atomization progress, and thus affect the engine combustion and emission performances. The experimental investigation of the early stage spray behaviors with biodiesel and diesel was carried out by employing a laser-based Mie-scattering method. The results show that the spray tip penetration for biodiesel is higher than that for diesel at the early stage spray under the same injection pressure. Moreover, the early stage spray tip penetration can be longer under high injection pressures for two fuels. Besides, the early stage spray cone angle for biodiesel is narrower than that for diesel, and the spray cone angle is especially higher than biodiesel by 25.8% after start of injection time of 0.01ms. Furthermore, under the same injection condition, the difference of early stage spray area between diesel and biodiesel is not obvious, while the spray volume for biodiesel is larger than that for diesel, and also the spray volume can be enlarged by increasing injection pressure for both fuels.This work was supported by the contribution of China postdoctoral fund projects [grant number2013M530236]; The projects of ‘Six talent peak’ [grant number 2014-ZBZZ-014]; Research start-up found projects of Jiangsu university [grant number 13JDG104]; Natural Science Foundation of Jiangsu Province of China [grant number BK20150520]；The Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD]Yu, S.; Yin, B.; Wen, S.; Li, X.; Jia, H.; Yu, J. (2017). Experimental Investigation on the Early Stage Spray Characteristics with Biodiesel and Diesel. En Ilass Europe. 28th european conference on Liquid Atomization and Spray Systems. Editorial Universitat Politècnica de València. 473-479. https://doi.org/10.4995/ILASS2017.2017.4651OCS47347

microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma.

Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR-33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma

Overexpression of long non-coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR-205-EGLN2 pathway.

Growing evidence suggests that long non-coding RNAs NORAD and miR-205 play a significant role in regulating cancer progression and metastasis. In this study, high expression of NORAD was firstly observed in melanoma tissues and human malignant melanoma cell lines, our aim was to study the interaction of them in the process of invasion and migration of malignant melanoma cells. NORAD, miR-205, and EGLN2 mRNA level in MM cells was detected by qRT-PCR. In situ hybridization (ISH) was performed to detect NORAD expression in MM tissues specimens. Effects of NORAD and miR-205 on Prolyl hydroxylase 2 (EGLN2) expression was explored by western blot in MM cells line. Dual-luciferase reporter assay was performed to verify the interaction relationship between NORAD and miR-205, as well as, miR-205 and EGLN2. Transwell assay was conducted to explore the effects of NORAD and miR-205 in vitro. Xenografts in nude mice experiment were used to confirm the role of NORAD and miR-205 in vivo. In vitro, NORAD knockdown significantly inhibited migration and invasion of malignant melanoma cells and elevated the expression of miR-205, there was an interaction between miR-205 and NORAD in the RNA-induced silencing complex. Upregulation of miR-205 induced significant inhibition of migratory and invasive ability compared with the scrambled control. However, downregulating NORAD largely reversed this effect. Furthermore, the regulatory effects of miR-205 on EGLN2 levels and the induction of endoplasmic reticulum stress were reversed by NORAD. In vivo, deletion of miR-205 induced tumor growth in nude mice. NORAD may play critical roles in tumorigenesis and progression of malignant melanoma by regulating of the miR-205-EGLN2 pathway, and may serve as a new therapeutic target

Genetic prediction of causal association between serum bilirubin and hematologic malignancies: a two-sample Mendelian randomized and bioinformatics study

IntroductionAn increasing number of cohort studies have shown a correlation between serum bilirubin and tumors, but no definitive causal relationship has been established between serum bilirubin and hematological malignancies.Therefore, the aim of the present study was to assess the causal relationship of serum bilirubin, including total bilirubin (TBIL) and direct bilirubin (DBIL), with hematological malignancies, including leukemia, lymphoma, and myeloma.MethodsWe used a genome-wide association study (GWAS) collection of TBIL, DBIL, and hematological malignancies data. Using two-sample Mendelian randomization(MR), we assessed the impact of TBIL and DBIL on hematological malignancies. For this study, the inverse variance weighting method (IVW) was the primary method of MR analysis. In the sensitivity analysis, the weighted median method, MR Egger regression, and MR-PRESSO test were used. To understand the mechanisms behind TBIL and DBIL, we used three different approaches based on screening single nucleotide polymorphisms (SNPs) and their associated genes, followed by bioinformatics analysis.ResultsThe IVW test results showed evidence of effects of TBIL (odds ratio [OR]: 4.47, 95% confidence interval [CI]: 1.58-12.62) and DBIL (OR: 3.31, 95% CI: 1.08-10.18) on the risk of acute myeloid leukemia (AML).The findings from bioinformatics indicated that TBIL could potentially undergo xenobiotic metabolism through cytochrome P450 and contribute to chemical carcinogenesis.DiscussionIn this study, two-sample MR analysis revealed a causal relationship between TBIL, DBIL, and AML

Adolescent mental health interventions: a narrative review of the positive effects of physical activity and implementation strategies

IntroductionThe psychological well-being of adolescents is a global concern due to increasing societal pressures and mental health issues. Physical activity is known to enhance physical health and has potential benefits for mental health, including reducing symptoms of anxiety and depression, boosting self-esteem, and improving social skills. This narrative review explores how physical activity can serve as an intervention to help adolescents manage psychological stress and prevent mental health issues.MethodsAn extensive literature search was conducted using databases such as PubMed, PsycINFO, Web of Science, and Scopus. Keywords included “adolescent mental health,” “physical activity,” “psychological intervention,” “types of exercise,” “anxiety,” “depression,” “self-esteem,” “social skills,” and “emotional regulation.” Studies were included based on relevance, peer-reviewed status, and involvement of adolescent populations. Data were extracted and analyzed qualitatively, focusing on the psychological impacts of different types of physical activity. Sixty one articles were eventually included.Results and conclusionThe review identified multiple studies highlighting the positive effects of various physical activities on adolescent mental health. Aerobic exercises were found to improve mood and cognitive function, strength training reduced depressive symptoms and increased self-efficacy, team sports enhanced social skills and a sense of community, and mind–body practices like yoga and tai chi improved stress management and emotional regulation. The findings suggest that physical activity can play a significant role in promoting adolescent mental health. Implementation strategies in school and community settings, including integrating physical activity into school curricula, offering diverse activity options, training professional instructors, encouraging family and community involvement, and regular monitoring and evaluation, are recommended. Future research should address limitations such as sample diversity and long-term effects. This narrative review underscores the importance of physical activity in enhancing adolescent mental health. Effective implementation strategies and multi-sector collaboration are essential for maximizing the benefits of physical activity interventions

Comparison of short-segment monoaxial and polyaxial pedicle screw fixation combined with intermediate screws in traumatic thoracolumbar fractures: a finite element study and clinical radiographic review

OBJECTIVES: No studies have compared monoaxial and polyaxial pedicle screws with regard to the von Mises stress of the instrumentation, intradiscal pressures of the adjacent segment and adjacent segment degeneration. METHODS: Short-segment monoaxial/polyaxial pedicle screw fixation techniques were compared using finite element methods, and the redistributed T11-L1 segment range of motion, largest maximal von Mises stress of the instrumentation, and intradiscal pressures of the adjacent segment under displacement loading were evaluated. Radiographic results of 230 patients with traumatic thoracolumbar fractures treated with these fixations were reviewed, and the sagittal Cobb’s angle, vertebral body angle, anterior vertebral body height of the fractured vertebrae and adjacent segment degeneration were calculated and evaluated. RESULTS: The largest maximal values of the von Mises stress were 376.8 MPa for the pedicle screws in the short-segment monoaxial pedicle screw fixation model and 439.9 MPa for the rods in the intermediate monoaxial pedicle screw fixation model. The maximal intradiscal pressures of the upper adjacent segments were all greater than those of the lower adjacent segments. The maximal intradiscal pressures of the monoaxial pedicle screw fixation model were larger than those in the corresponding segments of the normal model. The radiographic results at the final follow-up evaluation showed that the mean loss of correction of the sagittal Cobb’s angle, vertebral body angle and anterior vertebral body height were smallest in the intermediate monoaxial pedicle screw fixation group. Adjacent segment degeneration was less likely to be observed in the intermediate polyaxial pedicle screw fixation group but more likely to be observed in the intermediate monoaxial pedicle screw fixation group. CONCLUSION: Smaller von Mises stress in the pedicle screws and lower intradiscal pressure in the adjacent segment were observed in the polyaxial screw model than in the monoaxial pedicle screw fixation spine models. Fracture-level fixation could significantly correct kyphosis and reduce correction loss, and adjacent segment degeneration was less likely to be observed in the intermediate polyaxial pedicle screw fixation group

Gas geochemistry of the hot springs gas in Fujian province, SE China: insight into the deep faults and seismic activity

Fujian province is located at the forefront of the South China continental margin, situated on the edge of the Circum-Pacific seismic belt, and it is one of the regions with the most active neotectonic and geothermal activities in Chinese mainland. To explore the geochemical signals of hot spring gases to tectonic activity and earthquakes, a collection of geothermal gas samples was collected from 29 locations in Fujian from January 2021 to December 2022 (many of which were multiply collected at several sites quarterly). The gas samples were tested for their gas composition, helium, neon, carbon isotopes, radon contents, and gas flow rates. The results show that the dominant component of the hot spring outgassing is N2, and the increase in CO2 content is often associated with the increasing 13C. The variation range of the helium isotope ratio (3He/4He) in the hot spring gases is between 0.06 and 2.20Ra, and Rc/Ra varies between 0.06 and 1.58, with peak values occurring at the intersections of deep faults. Radon contents range from 18 to 2000 Bq/L. Calculations revealed that the maximum proportion of mantle-derived helium is 30.2%, and the mantle-derived heat contribution ranges from 37.6% to 63.4%. These data indicate a significant mantle degassing process in Fujian, with a high degree of mantle-crust connectivity, and mantle-derived heat as the main source of geothermal activity in the area. Comparative analysis with regional seismic activity indicates that areas with relatively strong upwelling of deep fluids are the main regions of regional seismic activity, and seismic intensity is positively correlated with mantle-derived heat flow. Thus, deep thermal fluid actives are closely genetically correlated to regional seismic activity. Additionally, the correlation analysis with the Taiwan ML6.0 earthquake suggests that high 3He/4He, δ13CCO2 values of hot spring gas and gas flow velocity in Nancheng Hot Spring (QZ6) indicate significant short-term and imminent anomaly indications preceding ML6.0 earthquakes in the Taiwan region

Low-temperature-flux syntheses of ultraviolet-transparent borophosphates Na4MB2P3O13 (M = Rb, Cs) exhibiting a second-harmonic generation response

The first non-centrosymmetric mixed-alkali-metal borophosphates, Na4MB2P3O13 (M = Rb 1, Cs 2), were obtained using a low-temperature flux method. Single-crystal X-ray diffraction studies of 1 and 2 reveal that the two compounds are isostructural, both crystallizing in the orthorhombic space group Pna21; their structures consist of novel 1D borophosphate chains constructed from B2P3O14 fundamental building units, assembled into a 3D framework by alkali metal cations. Second-harmonic generation (SHG) measurements show that 1 and 2 are type-I phase-matchable, with SHG responses ca. 0.35 and 0.42 times that of KH2PO4, respectively. The cut-off edges of 1 and 2 are ca. 276 and 267 nm, respectively, which suggests that they are potential ultraviolet nonlinear optical materials. Density functional theory calculations were employed to shed light on the band structure and density of states as well as the electron density distribution