SPIRAL 2 injector diagnostics

International audienceThe future SPIRAL2 facility will be composed of a multi-beam driver accelerator (5 mA/40 MeV deuterons, 5 mA /14.5 MeV/u heavy ions) and a dedicated building for the production of radioactive ion beams (RIBs). RIBs will be accelerated by the existing cyclotron CIME for the post acceleration and sent to GANIL's experimental areas. The injector constituted by an ion source a deuteron/proton source a L.E.B.T. and a M.E.B.T. lines and a room temperature R.F.Q. will produces, transports and accelerates beams up to an energy of 0.75 MeV/u. An Intermediate Test Bench (B.T.I.) is being built to commission the SPIRAL2 injector through the first rebuncher of the M.E.B.T. line in a first step and the last rebuncher in a second step. The B.T.I. is designed to perform a wide variety of measurements and functions and to go more deeply in the understanding of the behaviour of diagnostics under high average intensity beam operations. A superconducting LINAC equipped with two types of cavity will allow reaching 20 MeV/u for deuterons beam. This paper describes injector diagnostic developments and gives information about the current status

Development and implementation of a highly-multiplexed SNP array for genetic mapping in maritime pine and comparative mapping with loblolly pine

<p>Abstract</p> <p>Background</p> <p>Single nucleotide polymorphisms (SNPs) are the most abundant source of genetic variation among individuals of a species. New genotyping technologies allow examining hundreds to thousands of SNPs in a single reaction for a wide range of applications such as genetic diversity analysis, linkage mapping, fine QTL mapping, association studies, marker-assisted or genome-wide selection. In this paper, we evaluated the potential of highly-multiplexed SNP genotyping for genetic mapping in maritime pine (<it>Pinus pinaster </it>Ait.), the main conifer used for commercial plantation in southwestern Europe.</p> <p>Results</p> <p>We designed a custom GoldenGate assay for 1,536 SNPs detected through the resequencing of gene fragments (707 <it>in vitro </it>SNPs/Indels) and from Sanger-derived Expressed Sequenced Tags assembled into a unigene set (829 <it>in silico </it>SNPs/Indels). Offspring from three-generation outbred (G2) and inbred (F2) pedigrees were genotyped. The success rate of the assay was 63.6% and 74.8% for <it>in silico </it>and <it>in vitro </it>SNPs, respectively. A genotyping error rate of 0.4% was further estimated from segregating data of SNPs belonging to the same gene. Overall, 394 SNPs were available for mapping. A total of 287 SNPs were integrated with previously mapped markers in the G2 parental maps, while 179 SNPs were localized on the map generated from the analysis of the F2 progeny. Based on 98 markers segregating in both pedigrees, we were able to generate a consensus map comprising 357 SNPs from 292 different loci. Finally, the analysis of sequence homology between mapped markers and their orthologs in a <it>Pinus taeda </it>linkage map, made it possible to align the 12 linkage groups of both species.</p> <p>Conclusions</p> <p>Our results show that the GoldenGate assay can be used successfully for high-throughput SNP genotyping in maritime pine, a conifer species that has a genome seven times the size of the human genome. This SNP-array will be extended thanks to recent sequencing effort using new generation sequencing technologies and will include SNPs from comparative orthologous sequences that were identified in the present study, providing a wider collection of anchor points for comparative genomics among the conifers.</p

Mesures du courant faisceau sur l'accélérateur SPIRAL2

Le projet Spiral2 se compose d'un accélérateur et d'une section faisceaux radioactifs. L'accélérateur, composé principalement d'une source d'ions, d'une source deuton, d'un RFQ et d'un linac supraconducteur, pourra accélérer des faisceaux de 5 mA de deutons jusqu'à 40MeV et 1mA d'ions q/A= 1/3 jusqu'à 14,5MeV/u. Une nouvelle électronique a été développée au Ganil pour mesurer des courant provenant de diagnostiques interceptifs (Coupelles de Faraday) et non-interceptifs (Transformateur d'Intensité). Le but étant d'assurer des mesures de rendement, de suivi et de surveillance d'intensité Le principe employé consiste à effectuer une conversion linéaire (courant tension) permettant de faire des mesures de courant avec une grande précision sur une large gamme d'intensité.. Les diagnostiques et l'électronique sont présentés