Flora fanerogâmica da Serra Negra, Minas Gerais, Brasil

O presente estudo teve como objetivo caracterizar a flora fanerogâmica da região da Serra Negra localizada no sul da Zona da Mata de Minas Gerais, entre os municípios de Lima Duarte, Rio Preto, Santa Bárbara do Monte Verde e Olaria. Embora considerada de importância biológica alta, esta região não possui nenhum registro anterior de dados florísticos, o que levou ao desenvolvimento deste levantamento, durante o período de 2003 a 2010. A vegetação é caracterizada por um mosaico de formações florestais e campestres onde se destacam os campos rupestres e florestas nebulares em altitudes que variam de 1300 a ca. 1700 m. Um total de 1033 espécies foi encontrado, distribuídas em 469 gêneros e 121 famílias sendo as mais representativas Orchidaceae (115 spp.), Asteraceae 54 spp.), Melastomataceae (56 spp.), Myrtaceae (53 spp.), Fabaceae, Poaceae e Rubiaceae (48 spp. cada), Bromeliaceae (43 spp.), Solanaceae (38 spp.) e Piperaceae (33 spp). Novos registros e endemismos para a flora mineira foram encontrados e 58 espécies estão citadas na lista de espécies ameaçadas de Minas Gerais

alphav-beta3-integrin is a major sensor and activator of innate immunity to herpes simplex virus-1.

Pathogens are sensed by Toll-like receptors (TLRs) and a growing number of non-TLR receptors. Integrins constitute a family of signaling receptors exploited by viruses and bacteria to access cells. By gain- and loss-of-function approaches we found that αvβ3-integrin is a sensor of and plays a crucial role in the innate defense against herpes simplex virus (HSV). αvβ3-integrin signaled through two pathways. One concurred with TLR2, affected activation/induction of interferons type 1 (IFNs-1), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), and a polarized set of cytokines and receptors. The virion glycoproteins gH/gL sufficed to induce IFN1 and NF-κB via this pathway. The other pathway was TLR2-independent, involved sarcoma (SRC)-spleen tyrosine kinase (SYK)-Caspase recruitment domain-containing protein 9 (CARD9)-TRIF (TIR-domain-containing adapter-inducing interferon-β), and affected interferon regulatory factor 3 and 7 (IRF3-IRF7). The importance of αvβ3-integrin-mediated defense is reflected in the observation that HSV evolved the immediate-early infected cellular protein 0 (ICP0) protein to counteract it. We propose that αvβ3-integrin is considered a class of non-TLR pattern recognition receptors, a role likely exerted toward viruses and bacteria that interact with integrins and mount an innate response

Polymorphism On Codon 98 Of The Galectin-3 Gene Are Not Associated To Benign And Malignant Thyroid Tumors [o Polimorfismo No Códon 98 Do Gene De Galectina-3 Não Está Associado A Tumores Benignos E Malignos De Tiróide]

Galectin-3 is a multifunctional protein highly expressed in thyroid cancer. The galectin-3 gene (LGALS3) has several annotated candidates SNPs, however the relationship between galectin-3 SNPs and specific phenotypic variations relevant to health has not been evaluated. In this study, we investigated SNPs in the galectin-3 gene and a putative association with thyroid tumorigenesis. The presence of LGALS3 SNPs in thyroid carcinoma cell lines (NPA, TPC-1, WRO, ARO), thyroid tissues of 55 patients with multinodular goiter or papillary carcinoma diagnosis and lymphocytes of peripherical blood of 45 healthy individuals was evaluated by sequencing and SSCP. The analysis of LGALS3 coding sequence showed that the T98P site presents a great genotypic variation, since we observed both homozygous (AA or CC) and heterozygous (AC) patterns. In thyroid carcinoma cell lines, the genotype of NPA in the LGALS3 T98P site is CC, while TPC-1, WRO and ARO are AC. The genotypic frequency of T98P SNP observed in multinodular goiter (AC= 67%; AA= 23%; CC= 10%) and papillary carcinoma (AC= 68%; AA= 20%; CC= 12%) were similar to the frequency observed in the control population (AC= 60%, AA= 24%, CC=16%). In conclusion, no association between LGALS3 T98P genotype and the phenotype of the benign or malignant thyroid tumor was observed.50610751081Liu, F.T., Patterson, R.J., Wang, J.L., Intracellular functions of galectins (2002) Biochim Biophys Acta, 1572 (2-3), pp. 263-273Yoshii, T., Inohara, H., Takenaka, Y., Honjo, Y., Akahani, S., Nomura, T., Galectin-3 maintains the transformed phenotype of thyroid papillary carcinoma cells (2001) Int J Oncol, 18 (4), pp. 787-792Xu, X.C., el-Naggar, A.K., Lotan, R., Differential expression of galectin-1 and galectin-3 in thyroid tumors. Potential diagnostic implications (1995) Am J Pathol, 147 (3), pp. 815-822Inohara, H., Honjo, Y., Yoshii, T., Akahani, S., Yoshida, J., Hattori, K., Expression of galectin-3 in fine-needle aspirates as a diagnostic marker differentiating benign from malignant thyroid neoplasms (1999) Cancer, 85 (11), pp. 2475-2484Bartolazzi, A., Gasbarri, A., Papotti, M., Bussolati, G., Lucante, T., Khan, A., Application of an immunodiagnostic method for improving preoperative diagnosis of nodular thyroid lesions (2001) Lancet, 357 (9269), pp. 1644-1650Nascimento, M.C.P.A., Bisi, H., Alves, V.A.F., Longatto-Filho, A., Kanamura, C.T., Medeiros-Neto, G., Differential reactivity for galectin-3 in Hürthle cell adenomas and carcinomas (2001) Endocr Pathol, 2, pp. 275-279Saggiorato, E., Aversa, S., Deandreis, D., Arecco, F., Mussa, A., Puligheddu, B., Galectin-3: Presurgical marker of thyroid follicular epithelial cell-derived carcinomas (2004) J Endocrinol Invest, 27 (4), pp. 311-317Martins, L., Matsuo, S.E., Ebina, K.N., Kulcsar, M.A., Friguglietti, C.U., Kimura, E.T., Galectin-3 messenger ribonucleic acid and protein are expressed in benign thyroid tumors (2002) J Clin Endocrinol Metab, 87 (10), pp. 4806-4810Erichsen, H.C., Chanock, S.J., SNPs in cancer research and treatment (2004) Br J Cancer, 90 (4), pp. 747-751Kaklamani, V.G., Hou, N., Bian, Y., Reich, J., Offit, K., Michel, L.S., TGFBR1*6A and cancer risk: A meta-analysis of seven case-control studies (2003) J Clin Oncol, 21 (17), pp. 3236-3243Stephens, L.A., Powell, N.G., Grubb, J., Jeremiah, S.J., Bethel, J.A., Demidchik, E.P., Investigation of loss of heterozygosity and SNP frequencies in the RET gene in papillary thyroid carcinoma (2005) Thyroid, 15 (2), pp. 100-104Baumgartner-Parzer, S.M., Lang, R., Wagner, L., Heinze, G., Niederle, B., Kaserer, K., Polymorphisms in exon 13 and intron 14 of the RET protooncogene: Genetic modifiers of medullary thyroid carcinoma? (2005) J Clin Endocrinol Metab, 90 (11), pp. 6232-6236Granja, F., Morari, J., Morari, E.C., Correa, L.A.C., Assumpção, L.V.M., Ward, L.S., Proline homozygosity in codon 72 of p53 is a factor of susceptibility for thyroid cancer (2004) Cancer Lett, 210, pp. 151-157Basolo, F., Giannini, R., Faviana, P., Fontanini, G., Patricelli Malizia, A., Ugolini, C., Thyroid papillary carcinoma: Preliminary evidence for a germ-line single nucleotide polymorphism in the Fas gene (2004) J Endocrinol, 182, pp. 479-484Matakidou, A., Hamel, N., Popat, S., Henderson, K., Kantemiroff, T., Harmer, C., Risk of non-medullary thyroid cancer influenced by polymorphic variation in the thyroglobulin gene (2004) Carcinogenesis, 25 (3), pp. 369-373Cooper, D.N., Galectinomics: Finding themes in complexity (2002) Biochim Biophys Acta, 1572 (2-3), pp. 209-231Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs (1997) Nucleic Acids Res, 25 (17), pp. 3389-3402Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 199725:4876-82Guttmacher, A.E., Collins, F.S., Drazen, J.M., (2004) Genomic medicine: Articles from the New England Journal of Medicine, , Baltimore: The Johns Hopkins University PressGong, H.C., Honjo, Y., Nangia-Makker, P., Hogan, V., Mazurak, N., Bresalier, R.S., The NH2 terminus of galectin-3 governs cellular compartmentalization and functions in cancer cells (1999) Cancer Res, 59 (24), pp. 6239-624

Isotopic yield distribution of neutron-rich fragment nuclei produced in thermal neutron induced fission

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Fission fragment mass distribution in thermal neutron induced fission of 235^{235}U

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Excited 0+^+ states and deformed structures in the transitional nucleus 98^{98}Zr

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Co-existing excitation modes in neutron rich nucleus- 98^{98}Zr

International audienceNeutron-rich nuclei in A∼100 mass region exhibits a variety of nuclear structure phenomena such as the existence of different excited 0+ deformed states, shape coexisting states, high spin single-particle isomeric states, octupole vibrational states etc [1,2]. We are reporting here the different coexisting excitation modes in the medium spin structures of 98Zr nucleus with the incorporation of the new spectroscopic results obtained from an experiment using the thermal neutron induced fission of 235U

Low- and Medium-Spin Level Structure of neutron rich 96^{96}Sr: Competition between Vibrational and. Rotational modes of excitations

International audienceNuclei in A ∼ 100 mass region exhibit large variety of interesting nuclear structure phenomena [1]. It has been observed that the nuclei with N ≤ 58 display features that characterize a spherical vibrator (see Fig. 1). On the other hand, nuclei with N ≥ 60 exhibit deformed rotor like behavior. It is obvious from Fig. 1(c) that there is a sudden increase in deformation along the chain of even-even Srisotopes as the neutron number increases from N = 58 to N = 60. Also, it is quite interesting to note that the deformation gets saturated for Sr-isotopes lying in the more neutron-rich side of 96Sr. The sudden onset of deformation and its immediate saturation at N ∼ 60 can be explained by the the occupation of the valence nucleons at the g9/2 proton and h11/2 neutron orbitals. Lying in the vicinity of highly deformed and nearly spherical region, 96Sr occupies a critical position in the nuclide chart where the onset of multifaceted excitation modes can be expected. We are reporting here nuclear structure phenomena associated with 96Sr in the low- and medium-spin regime following new spectroscopic information, obtained from an experiment employing thermal B(E2: 2+ → 0 +) values, and (c) β2 values, as a function of mass number, A are shown