3,905 research outputs found
A Pan1/End3/Sla1 complex links Arp2/3-mediated actin assembly to sites of clathrin-mediated endocytosis.
More than 60 highly conserved proteins appear sequentially at sites of clathrin-mediated endocytosis in yeast and mammals. The yeast Eps15-related proteins Pan1 and End3 and the CIN85-related protein Sla1 are known to interact with each other in vitro, and they all appear after endocytic-site initiation but before endocytic actin assembly, which facilitates membrane invagination/scission. Here we used live-cell imaging in parallel with genetics and biochemistry to explore comprehensively the dynamic interactions and functions of Pan1, End3, and Sla1. Our results indicate that Pan1 and End3 associate in a stable manner and appear at endocytic sites before Sla1. The End3 C-terminus is necessary and sufficient for its cortical localization via interaction with Pan1, whereas the End3 N-terminus plays a crucial role in Sla1 recruitment. We systematically examined the dynamic behaviors of endocytic proteins in cells in which Pan1 and End3 were simultaneously eliminated, using the auxin-inducible degron system. The results lead us to propose that endocytic-site initiation and actin assembly are separable processes linked by a Pan1/End3/Sla1 complex. Finally, our study provides mechanistic insights into how Pan1 and End3 function with Sla1 to coordinate cargo capture with actin assembly
Development and validation of the crew-station system-integration research facility
The various issues associated with the use of integrated flight management systems in aircraft were discussed. To address these issues a fixed base integrated flight research (IFR) simulation of a helicopter was developed to support experiments that contribute to the understanding of design criteria for rotorcraft cockpits incorporating advanced integrated flight management systems. A validation experiment was conducted that demonstrates the main features of the facility and the capability to conduct crew/system integration research
Simulating spin-charge separation with light
In this work we show that stationary light-matter excitations generated
inside a hollow one-dimensional waveguide filled with atoms, can be made to
generate a photonic two-component Lieb Liniger model. We explain how to prepare
and drive the atomic system to a strongly interacting regime where spin-charge
separation could be possible. We then proceed by explaining how to measure the
corresponding effective spin and charge densities and velocities through
standard optical methods based in measuring dynamically the emitted photon
intensities or by analyzing the photon spectrum. The relevant interactions
exhibit the necessary tunability both to generate and efficiently observe spin
charge separation with current technology.Comment: 4 pages. Comments welcom
Hematological response in sheep given protracted exposures to Co 60 gamma radiation
Leukocyte count changes in sheep after prolonged exposure to gamma irradiation at rate of 1.9 R/h
Sub-Nyquist Field Trial Using Time Frequency Packed DP-QPSK Super-Channel Within Fixed ITU-T Grid
Sub-Nyquist time frequency packing technique was demonstrated for the first
time in a super channel field trial transmission over long-haul distances. The
technique allows a limited spectral occupancy even with low order modulation
formats. The transmission was successfully performed on a deployed Australian
link between Sydney and Melbourne which included 995 km of uncompensated SMF
with coexistent traffic. 40 and 100 Gb/s co-propagating channels were
transmitted together with the super-channel in a 50 GHz ITU-T grid without
additional penalty. The super-channel consisted of eight sub-channels with
low-level modulation format, i.e. DP-QPSK, guaranteeing better OSNR robustness
and reduced complexity with respect to higher order formats. At the receiver
side, coherent detection was used together with iterative maximum-a-posteriori
(MAP) detection and decoding. A 975 Gb/s DP-QPSK super-channel was successfully
transmitted between Sydney and Melbourne within four 50GHz WSS channels (200
GHz). A maximum potential SE of 5.58 bit/s/Hz was achieved with an OSNR=15.8
dB, comparable to the OSNR of the installed 100 Gb/s channels. The system
reliability was proven through long term measurements. In addition, by closing
the link in a loop back configuration, a potential SE*d product of 9254
bit/s/Hz*km was achieved
A millimeter-wave antireflection coating for cryogenic silicon lenses
We have developed and tested an antireflection (AR) coating method for
silicon lenses at cryogenic temperatures and millimeter wavelengths. Our
particular application is a measurement of the cosmic microwave background. The
coating consists of machined pieces of Cirlex glued to the silicon. The
measured reflection from an AR coated flat piece is less than 1.5% at the
design wavelength. The coating has been applied to flats and lenses and has
survived multiple thermal cycles from 300 to 4 K. We present the manufacturing
method, the material properties, the tests performed, and estimates of the loss
that can be achieved in practical lenses
Spinons and Holons with Polarized Photons in a Nonlinear Waveguide
We show that the spin-charge separation predicted for correlated fermions in
one dimension, could be observed using polarized photons propagating in a
nonlinear optical waveguide. Using coherent control techniques and employing a
cold atom ensemble interacting with the photons, large nonlinearities in the
single photon level can be achieved. We show that the latter can allow for the
simulation of a strongly interacting gas, which is made of stationary
dark-state polaritons of two species and then shown to form a Luttinger liquid
of effective fermions for the right regime of interactions. The system can be
tuned optically to the relevant regime where the spin-charge separation is
expected to occur. The characteristic features of the separation as
demonstrated in the different spin and charge densities and velocities can be
efficiently detected via optical measurements of the emitted photons with
current optical technologies.Comment: To appear in New Journal of Physic
Design and fabrication of 3D-printed anatomically shaped lumbar cage for intervertebra disc (IVD) degeneration treatment
Spinal fusion is the gold standard surgical procedure for degenerative spinal conditions when conservative therapies have been unsuccessful in rehabilitation of patients. Novel strategies are required to improve biocompatibility and osseointegration of traditionally used materials for lumbar cages. Furthermore, new design and technologies are needed to bridge the gap due to the shortage of optimal implant sizes to fill the intervertebral disc defect. Within this context, additive manufacturing technology presents an excellent opportunity to fabricate ergonomic shape medical implants. The goal of this study is to design and manufacture a 3D-printed lumbar cage for lumbar interbody fusion. Optimisations of the proposed implant design and its printing parameters were achieved via in silico analysis. The final construct was characterised via scanning electron microscopy, contact angle, x-ray micro computed tomography (μCT), atomic force microscopy, and compressive test. Preliminary in vitro cell culture tests such as morphological assessment and metabolic activities were performed to access biocompatibility of 3D-printed constructs. Results of in silico analysis provided a useful platform to test preliminary cage design and to find an optimal value of filling density for 3D printing process. Surface characterisation confirmed a uniform coating of nHAp with nanoscale topography. Mechanical evaluation showed mechanical properties of final cage design similar to that of trabecular bone. Preliminary cell culture results showed promising results in terms of cell growth and activity confirming biocompatibility of constructs. Thus for the first time, design optimisation based on computational and experimental analysis combined with the 3D-printing technique for intervertebral fusion cage has been reported in a single study. 3D-printing is a promising technique for medical applications and this study paves the way for future development of customised implants in spinal surgical applications
Low-complexity BCH codes with optimized interleavers for DQPSK systems with laser phase noise
The presence of high phase noise in addition to additive white Gaussian noise in coherent optical systems affects the performance of forward error correction (FEC) schemes. In this paper, we propose a simple scheme for such systems, using block interleavers and binary Bose–Chaudhuri–Hocquenghem (BCH) codes. The block interleavers are specifically optimized for differential quadrature phase shift keying modulation. We propose a method for selecting BCH codes that, together with the interleavers, achieve a target post-FEC bit error rate (BER). This combination of interleavers and BCH codes has very low implementation complexity. In addition, our approach is straightforward, requiring only short pre-FEC simulations to parameterize a model, based on which we select codes analytically. We aim to correct a pre-FEC BER of around (Formula presented.). We evaluate the accuracy of our approach using numerical simulations. For a target post-FEC BER of (Formula presented.), codes selected using our method result in BERs around 3(Formula presented.) target and achieve the target with around 0.2 dB extra signal-to-noise ratio
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