Dyslipidemia in systemic lupus erythematosus

Ph.DDOCTOR OF MEDICIN

A cross-sectional study on level of knowledge and level of attitude towards asthma among the sampled population aged 13 years and above of Rumah Uming and Rumah Cherida, Meradong, from 20th November 2006 to 26th January 2007

Introduction: Asthma is a chronic inflammatory disorder of the airways characterized by wheezing, breathlessness, chest tightness and cough. Asthma affects people of all ages. In Malaysia, asthma was among the commonest conditions that accounted for outpatient department attendance. Asthma is not curable, yet an asthmatic patient can live a normal life ifit is well-controlled. Objectives: The objective of this study is to study the level of knowledge and level of attitude towards asthma among the sampled population aged 13 years and above in Rumah Uming and Rumah Cherida from 20th November 2006 to 26th January 2007. Methodology: A cross-sectional study on level of knowledge and level of attitude towards asthma was carried out. The sample size of 90 respondents was chosen using a simple random sampling method. Data collection was done by means of interview-guided questionnaire, and all collected data was entered and analysed using SPSS software version 13. The statistical tests that were used include Pearson correlation test, chi-square test, independent sample t-test, ANOV A test, Spearman rho test, Mann-Whitney U test and Kruskal-Wallis test. Results: The main source of information regarding asthma was obtained from mass media. The study showed more than half of the respondents had poor level of knowledge in terms of general knowledge and signs and symptoms (62.2%), risk factor and triggering factor (55.6%), and treatment and prevention of asthma (60.0%). However, 56.7% of respondents had good knowledge about complications of asthma. There was a significant relationship between total level of knowledge with gender (p = 0.034), household income (p = 0.018), and personal and family history of asthma (p = 0.008). Females, higher income group and respondents with personal and family history of asthma showed better score of knowledge. Most of the respondents had good attitude in terms of seeking knowledge (65.6%) and prevention of asthma (53.3%), however 55.6% showed poor attitude in terms of the treatment of asthma. Significant association was found between total level of attitude and level of education (p = 0.042). There was also a positive relationship between the total level of knowledge and the total level of attitude towards asthma among the respondents (p = 0.023). Conclusion: The study revealed a significantly higher level of knowledge among the respondents who were female, with higher household income and with personal and family history of asthma

Characterization of quorum sensing genes and N-acyl homoserine lactones in Citrobacter amalonaticus strain YG6

In the phylum of Proteobacteria, quorum sensing (QS) system is widely driven by synthesis and response of N-acyl homoserine lactone (AHL) signalling molecules. AHL is synthesized by LuxI homologue and sensed by LuxR homologue. Once the AHL concentration achieves a threshold level, it triggers the regulation of target genes. In this study, QS activity of Citrobacter amalonaticus strain YG6 which was isolated from clams was investigated. In order to characterise luxI/R homologues, the genome of C. amalonaticus strain YG6 (4.95 Mbp in size) was sequenced using Illumina MiSeq sequencer. Through in silico analysis, a pair of canonical luxI/R homologues and an orphan luxR homologue were identified and designated as camI, camR, and camR2, respectively. A putative lux box was identified at the upstream of camI. The camI gene was cloned and overexpressed in E. coli BL21 (DE3)pLysS. High-resolution triple quadrupole liquid chromatography mass spectrometry (LC-MS/MS) analysis verified that the CamI is a functional AHL synthase which produced multiple AHL species, namely N‑butyryl‑L‑homoserine lactone (C4-HSL), N‑hexanoyl‑L‑homoserine lactone (C6-HSL), N‑octanoyl‑L‑homoserine lactone (C8-HSL), N‑tetradecanoyl‑L‑homoserine lactone (C14-HSL) and N‑hexadecanoyl‑L‑homoserine lactone (C16-HSL) in C. amalonaticus strain YG6 and camI gene in recombinant E. coli BL21(DE3)pLysS. To our best knowledge, this is the first functional study report of camI as well as the first report describing the production of C14-HSL by C. amalonaticus

Quality of life in an urban Asian population: the impact of ethnicity and socio-economic status

The relationships between ethnicity, socio-economic status (SES) and health-related quality of life (HRQoL) have not been well characterised in most Asian populations. We therefore studied the influence of ethnicity and SES on HRQoL in a multi-ethnic urban Asian population, adjusting for the influence of other known determinants of HRQoL. In a disproportionately stratified, cross-sectional, population-based survey, Chinese, Malay and Indian subjects in Singapore completed the Short Form 36 Health Survey (SF-36) HRQoL measure and were assessed to determine demographic, socio-economic, psychosocial and other characteristics. Multiple linear regression models were used to study the influence of ethnicity and SES on SF-36 scores while adjusting for the influence of other determinants of HRQoL. The survey participation rate was 92.8%. Ethnic differences in HRQoL were present for all 8 SF-36 scales (pHealth-related quality of life Ethnicity Socio-economic status Asia Singapore

Recommendations for COVID-19 vaccination in people with rheumatic disease: Developed by the Singapore Chapter of Rheumatologists

10.1111/1756-185X.14107INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES246746-75