On Developing Human Centric Digital Twins in Industry 4.0 and Beyond

The use of smart devices has increased greatly in the last ten years with users reaching out to the possibility to do more with them especially in the networking front. In this context there is a need to understand the connection between users’ social demographic factors, their way to related to their smart devices, and the increasing adoption smart technologies in the industry 4.0 domains. This study was designed to evaluate the senso of belonging of a community using smart devices. In this way we evaluate intangible benefits that employees may gain from a more immerse relationship with their devices. We used a dataset of 586 anonymous respondent of an existing survey designed for capturing the relationships that humans develop with their smart devices. In particular, we investigate the relationships with smart device and particular background variables of the respondents using a chi-square test. The study showed that there is a significant relationship between users’ sex and smart device type and their dependency on smart device. Both male and females, tends to think that smart device enables them to connect with a larger community and smart phone users connect more to large community than other smart devices users. This study provided several significant findings that strengthen the previous literature works on subject matter. Certain socio demographics variables like age, sex and smart device type showed a correlation between smart device users and their tendency to stay in touch with a larger community via their smart device.</p

A promising new ELISA diagnostic test for cattle babesiosis based on Babesia bigemina Apical Membrane Antigen-1.

Babesiosis due to Babesia bigemina is a relevant tick‑borne disease, affecting cattle worldwide. Many surface proteins of the pathogen including the Apical Membrane Antigen 1 (AMA‑1) ‑ have been analysed for vaccine and diagnostic purposes. This study focused on B. bigemina AMA‑1 and on its use for the assessment of diagnostic tests. After bioinformatic analyses, AMA‑1 codifying region was amplified and cloned into an expression vector used to induce protein synthesis in Escherichia coli cells. AMA‑1 was purified by affinity chromatography and used to set up the best condition for an ELISA protocol. Bovine field sera positive to B. bigemina were used to evaluate the presence of anti‑AMA‑1 antibodies. In order to verify the assay specificity, sera positive to Babesia bovis or to the piroplasm Theileria annulata were also included. Significant differences were obtained between sera negative to both B. bigemina and B. bovis and samples positive to B. bigemina, to B. bovis or to both pathogens. No significant reaction was observed with T. annulata positive sera. The results showed that AMA‑1 protein is suitable to be used as antigen in diagnostic assays for babesiosis diagnosis in cattle, as it does not show any cross reaction with anti-T. annulata antibodies

Assembly line balancing and activity scheduling for customised products manufacturing

Nowadays, end customers require personalized products to match their specific needs. Thus, production systems must be extremely flexible. Companies typically exploit assembly lines to manufacture produces in great volumes. The development of assembly lines distinguished by mixed or multi models increases their flexibility concerning the number of product variants able to be manufactured. However, few scientific contributions deal with customizable products, i.e., produces which can be designed and ordered requiring or not a large set of available accessories. This manuscript proposes an original two-step procedure to deal with the multi-manned assembly lines for customized product manufacturing. The first step of the procedure groups the accessories together in clusters according to a specific similarity index. The accessories belonging to a cluster are typically requested together by customers and necessitate a significant mounting time. Thus, this procedure aims to split accessories belonging to the same cluster to different assembly operators avoiding their overloads. The second procedure step consists of an innovative optimization model which defines tasks and accessory assignment to operators. Furthermore, the developed model defines the activity time schedule in compliance with the task precedencies maximizing the operator workload balance. An industrial case study is adopted to test and validate the proposed procedure. The obtained results suggest superior balancing of such assembly lines, with an average worker utilization rate greater than 90%. Furthermore, in the worst case scenario in terms of customer accessories requirement, just 4 line operators out of 16 are distinguished by a maximum workload greater than the cycle time

Grid computing technologies for renewable electricity generator monitoring and control

In this paper we discuss the use of real-time Grid computing for the monitoring, control and simulation of renewable electricity generators and their associated electrical networks. We discuss briefly the architectural design of GRIDCC and how we have integrated a number of real (solar, CHP) and simulated conventional power generators into the GRIDCC environment. A local weather station has also been attached to an Instrument Manager to alert experts appropriately when the Solar Array is not generating. The customised remote control and monitoring environment (a virtual control room), distributed using a standard web server, is discussed

Microbiological and serological monitoring in hooded crow (Corvus corone cornix) in the Region Lombardia, Italy

The health status of 276 hooded crows (Corvus corone cornix) from various provinces of Lombardy was monitored for three years. Bacteriological examination detected E. coli (76%), Campylobacter jejuni (17%), Salmonella typhimurium (11.6%), Yersinia spp. (6.5%), Clamydophila abortus and C. psittaci (2.6%); from six birds showing severe prostration Pasteurella multocida was isolated. Virological and serological tests were negative for Avian Influenza virus (AIV), West Nile virus (WNV) and only three samples were positive for Newcastle disease virus (NDV) but only at serology (titre 1:16)

Malin 1: interacting galaxy pair?

Malin 1 is a unique, extraordinarily large low surface brightness galaxy. The structure and the origins of the galaxy are poorly understood. The reason for such a situation is an absence of detailed observational data, especially, of high-resolution kinematics. In this Letter we study the stellar kinematics of the inner part (r < 15 kpc) of Malin 1. We present spectroscopic arguments in favour of a small galaxy - Malin 1B - being a companion probably interacting with the main galaxy - Malin 1. This object is clearly seen in many published images of Malin 1 but is not mentioned in any astronomical databases. Malin 1B is located at the projected distance of 14 kpc from the Malin 1's nucleus and has small - 65$\pm$16 km/s - relative velocity, which we determined for the first time. We suggest that ongoing interaction with Malin 1B can explain main morphological features of the Malin 1's central region - two-armed spiral structure, a bar, and an external one-armed spiral pattern. We also investigated the large scale environment of Malin 1 and postulate that the galaxy SDSS J123708.91+142253.2 might be responsible for the formation of extended low-surface brightness envelope by means of head-on collision with Malin 1 (in the framework of collision scenario proposed by Mapelli et al. 2008). To test the collisional origins of Malin 1 global structure, more observational data and new numerical models are needed.Comment: 5 pages, 4 figures, accepted for publication in MNRA

Target-Selective Drug Delivery through Liposomes Labeled with Oligobranched Neurotensin Peptides.

The structure and the in vitro behavior of liposomes filled with the cytotoxic drug doxorubicin (Doxo) and functionalized on the external surface with a branched moiety containing four copies of the 8-13 neurotensin (NT) peptide is reported. The new functionalized liposomes, DOPC-NT(4) Lys(C(18) )(2) , are obtained by co-aggregation of the DOPC phospholipid with a new synthetic amphiphilic molecule, NT(4) Lys(C(18) )(2) , which contains a lysine scaffold derivatized with a lipophilic moiety and a tetrabranched hydrophilic peptide, NT8-13, a neurotensin peptide fragment well known for its ability to mimic the neurotensin peptide in receptor binding ability. Dynamic light scattering measurements indicate a value for the hydrodynamic radius (RH) of 88.3±4.4 nm. The selective internalization and cytotoxicity of DOPC-NT(4) Lys(C(18) )(2) liposomes containing Doxo, as compared to pure DOPC liposomes, were tested in HT29 human colon adenocarcinoma and TE671 human rhabdomyosarcoma cells, both of which express neurotensin receptors. Peptide-functionalized liposomes show a clear advantage in comparison to pure DOPC liposomes with regard to drug internalization in both HT29 and TE671 tumor cells: FACS analysis indicates an increase in fluorescence signal of the NT(4) -liposomes, compared to the DOPC pure analogues, in both cell lines; cytotoxicity of DOPC-NT(4) Lys(C(18) )(2) -Doxo liposomes is increased four-fold with respect to DOPC-Doxo liposomes in both HT29 and TE671 cell lines. These effects could to be ascribed to the higher rate of internalization for DOPC-NT(4) Lys(C(18) )(2) -Doxo liposomes, due to stronger binding driven by a lower dissociation constant of the NT(4) -liposomes that bind the membrane onto a specific protein, in contrast to DOPC liposomes, which approach the plasma membrane unselectively

Do dogs and cats passively carry sars-cov-2 on hair and pads?

The epidemiological role of domestic animals in the spread and transmission of SARS-CoV-2 to humans has been investigated in recent reports, but some aspects need to be further clarified. To date, only in rare cases have dogs and cats living with COVID-19 patients been found to harbour SARS-CoV-2, with no evidence of pet-to-human transmission. The aim of the present study was to verify whether dogs and cats act as passive mechanical carriers of SARS-CoV-2 when they live in close contact with COVID-19 patients. Cutaneous and interdigital swabs collected from 48 dogs and 15 cats owned by COVID-19 patients were tested for SARS-CoV-2 by qRT-PCR. The time elapsed between owner swab positivity and sample collection from pets ranged from 1 to 72 days, with a median time of 23 days for dogs and 39 days for cats. All samples tested negative, suggesting that pets do not passively carry SARS-CoV-2 on their hair and pads, and thus they likely do not play an important role in the virus transmission to humans. This data may contribute to confirming that the direct contact with the hair and pads of pets does not represent a route for the transmission of SARS-CoV-2

GEOCHEMICAL INVESTIGATION OF AQUIFER POLLUTION FROM WASTE MANAGEMENT. THE CASE OF KOMOTINI LANDFILL (GREECE)

According to European legislation, environmental control and monitoring of landfills has become of crucial importance. This study includes a thorough geochemical approach aiming to evaluate the environmental impact of the landfill of Komotini, N. Greece. Samples of waters were taken from inside the working landfill as well as from the area of the neighbouring old landfill. The waters were analyzed chemically (major elements and heavy metals) and isotopically (D and 18O). Also, biogas flow was measured and the ratio CH4/CO2. Based on the geomorphological, hydrogeological and land use data of the area, we proceeded to analyses of waters both from the area of the landfill and from the wider region (drainage basin). The obtained results were used to construct digital maps (GIS) in order to determine the special dispersion of the polluted aquifers. The biogas flow in the old and new garbage burial sites was measured by accumulation chamber device for methane and carbon dioxide ratio determination. The obtained results show an important agent of pollution in the water samples downstream from the landfill and in a distance more than 2km, along the dispersion of the leachate. The land use of the area was taken into account to evaluate the importance and the criticality of the situation