151 research outputs found
Synthesis of novel sulfide-based cyclic peptidomimetic analogues to solonamides
Eight new sulfide-based cyclic peptidomimetic analogues of solonamides A and B have been synthesized via solid-phase peptide
synthesis and SN2’ reaction on a Morita–Baylis–Hillman (MBH) residue introduced at the N-terminal of a tetrapeptide. This last
step takes advantage of the electrophilic feature of the MBH residue and represents a new cyclization strategy occurring. The analogues were prepared in moderate overall yields and did not show toxic effects on Staphylococcus aureus growth and were not
toxic to human fibroblasts. Two of them inhibited the hemolytic activity of S. aureus, suggesting an interfering action in the bacterial quorum sensing similar to the one already reported for solonamides
Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine
The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries
Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine
The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries
Pathogenicity index, production of hemolysin and serogroup of samples of Escherichia coli isolated from commercial layng hens
Este estudo avaliou o índice de patogenicidade, a produção de hemolisina e a determinação de sorogrupos de cepas de Escherichia coli isoladas de fígado de aves de postura comercial com um dia de idade. Para este estudo, foram analisados 32 lotes, dos quais 15 foram positivos para o\ud
isolamento de E. coli no fígado, totalizando vinte e quatro amostras. A patogenicidade dos isolados foi determinada por inoculação no saco aéreo de pintinhos e classificada como alta, intermediária, baixa ou não-patogênica. Os sorogrupos foram identificados utilizando um conjunto de antissoros anti-O (O1 a O180). A produção de hemolisina foi determinada por semeadura em ágar sangue de galinha (8%) e em placas de ágar sangue de carneiro (8%). Do total de amostras estudadas, 17 (70,83%) foram classificadas como não patogênica, 6 (25%) como de baixa patogenicidade e 1 (4,17%) de alta patogenicidade. Foram identificados 14 sorogrupos diferentes: O1, O2, O5, O8, O15, O18, O22, O36, O64, O70, O75, O115, O132, O141. Cinco cepas não tiveram o sorogrupo identificado. Com relação ao teste de produção de hemolisina, todas as cepas foram consideradas negativas, tanto para o teste realizado com ágar sangue de galinha quanto para o de carneiro. Os resultados obtidos neste estudo demonstram a importância de se identificar as cepas prevalentes de E. coli nas diferentes regiões produtoras, podendo ser utilizados em estudos epidemiológicos.This work evaluated the index of pathogenicity, the production of hemolysin and determination of serogroups in Escherichia coli strains isolated from liver of commercial laying hens with one day of age. Thirtytwo lots were analyzed, of which 15 were positive for the isolation of E. coli in the liver, for a total of 24 samples. The pathogenicity in one-day-old chicks was determined by inoculation in air sac and was classified as high, intermediate or low pathogenicity, or non-pathogenic. Serogroups were identified using a set of anti-O antisera (O1 to O180). The production of hemolysin was determined by plating on chicken blood agar (8%) and sheep blood agar (8%). Of the samples studied, 17 (70.83%) were classified as non-pathogenic, 6 (25%) as low pathogenicity and 1 (4.17%) as high pathogenicity. Fourteen different serogroups were identified: O1, O2, O5, O8, O15, O18, O22, O36, O64, O70, O75, O115, O132 and O141, while 5 samples were non-typable. Regarding the test for production of hemolysin, all strains were considered negative for both the test performed with chicken blood agar and that with sheep blood agar. The results of this study demonstrate the importance of identifying the prevalent strains of E. coli in different producing regions, as this information can be used in epidemiological studie
Potential biological properties of lycopene in a self-emulsifying drug delivery system
In recent years, lycopene has been highlighted due to its antioxidant and anti-inflammatory properties, associated with a beneficial effect on human health. The aim of this study was to advance the studies of antioxidant and anti-inflammatory mechanisms on human keratinocytes cells (HaCaT) of a self-emulsifying drug delivery system (SEDDS) loaded with lycopene purified from red guava (nanoLPG). The characteristics of nanoLPG were a hydrodynamic diameter of 205 nm, a polydispersity index of 0.21 and a zeta potential of −20.57, providing physical stability for the nanosystem. NanoLPG demonstrated antioxidant capacity, as shown using the ORAC methodology, and prevented DNA degradation (DNA agarose). Proinflammatory activity was evaluated by quantifying the cytokines TNF-α, IL-6 and IL-8, with only IL-8 showing a significant increase (p < 0.0001). NanoLPG showed greater inhibition of the tyrosinase and elastase enzymes, involved in the skin aging process, compared to purified lycopene (LPG). In vitro treatment for 24 h with 5.0 µg/mL of nanoLPG did not affect the viability of HaCaT cells. The ultrastructure of HaCaT cells demonstrated the maintenance of morphology. This contrasts with endoplasmic reticulum stresses and autophagic vacuoles when treated with LPG after stimulation or not with LPS. Therefore, the use of lycopene in a nanoemulsion may be beneficial in strategies and products associated with skin health.info:eu-repo/semantics/publishedVersio
The NMR-derived solution structure of a new cationic antimicrobial peptide from the skin secretion of the anuran Hyla punctata.
Amphibian skin secretions constitute an important source of molecules for antimicrobial drug research in order to combat the increasing resistance of pathogens to conventional antibiotics. Among the various types of substances secreted by the dermal granular amphibian glands, there is a wide range of peptides and proteins, often displaying potent antimicrobial activities and providing an effective defense system against parasite infection. In the present work, we report the NMR solution structure and the biological activity of a cationic 14-residue amphiphilic alpha-helical polypeptide named Hylaseptin P1 (HSP1), isolated from the skin secretion of the hylid frog Hyla punctata. The peptide antimicrobial activity was verified against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, whereas no significant lytic effect was detected toward red or white blood cells
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity
Following the treads of our previous works on the unveiling of bioactive peptides encrypted
in plant proteins from diverse species, the present manuscript reports the occurrence of four
proof-of-concept intragenic antimicrobial peptides in human proteins, named Hs IAPs.
These IAPs were prospected using the software Kamal, synthesized by solid phase chemistry,
and had their interactions with model phospholipid vesicles investigated by differential
scanning calorimetry and circular dichroism. Their antimicrobial activity against bacteria,
yeasts and filamentous fungi was determined, along with their cytotoxicity towards erythrocytes.
Our data demonstrates that Hs IAPs are capable to bind model membranes while
attaining α-helical structure, and to inhibit the growth of microorganisms at concentrations
as low as 1μM. Hs02, a novel sixteen residue long internal peptide (KWAVRIIRKFIKGFISNH2)
derived from the unconventional myosin 1h protein, was further investigated in its
capacity to inhibit lipopolysaccharide-induced release of TNF-α in murine macrophages.
Hs02 presented potent anti-inflammatory activity, inhibiting the release of TNF-α in LPSprimed
cells at the lowest assayed concentration, 0.1 μM. A three-dimensional solution
structure of Hs02 bound to DPC micelles was determined by Nuclear Magnetic Resonance.
Our work exemplifies how the human genome can be mined for molecules with biotechnological
potential in human health and demonstrates that IAPs are actual alternatives to antimicrobial
peptides as pharmaceutical agents or in their many other putative applications
Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications
Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.info:eu-repo/semantics/publishedVersio
Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth
Background: Nc886 is a 102 bp non-coding RNA transcript initially classified as a microRNA precursor (Pre-miR-886), later as a divergent homologue of the vault RNAs (vtRNA 2-1) and more recently as a novel type of RNA (nc886). Although nc886/vtRNA2-1/Pre-miR-886 identity is still controversial, it was shown to be epigenetically controlled, presenting both tumor suppressor and oncogenic function in different cancers. Here, we study for the first time the role of nc886 in prostate cancer. Methods: Nc886 promoter methylation status and its correlation with patient clinical parameters or DNMTs levels were evaluated in TCGA and specific GEO prostate tissue datasets. Nc886 level was measured by RT-qPCR to compare normal/neoplastic prostate cells from radical prostatectomies and cell lines, and to assess nc886 response to demethylating agents. The effect of nc886 recovery in cell proliferation (in vitro and in vivo) and invasion (in vitro) was evaluated using lentiviral transduced DU145 and LNCaP cell lines. The association between the expression of nc886 and selected genes was analyzed in the TCGA-PRAD cohort. Results: Nc886 promoter methylation increases in tumor vs. normal prostate tissue, as well as in metastatic vs. normal prostate tissue. Additionally, nc886 promoter methylation correlates with prostate cancer clinical staging, including biochemical recurrence, Clinical T-value and Gleason score. Nc886 transcript is downregulated in tumor vs. normal tissue -in agreement with its promoter methylation status- and increases upon demethylating treatment. In functional studies, the overexpression of nc886 in the LNCaP and DU145 cell line leads to a decreased in vitro cell proliferation and invasion, as well as a reduced in vivo cell growth in NUDE-mice tumor xenografts. Finally, nc886 expression associates with the prostate cancer cell cycle progression gene signature in TCGA-PRAD. Conclusions: Our data suggest a tumor suppressor role for nc886 in the prostate, whose expression is epigenetically silenced in cancer leading to an increase in cell proliferation and invasion. Nc886 might hold clinical value in prostate cancer due to its association with clinical parameters and with a clinically validated gene signature
Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.Por meio de técnicas cromatográficas, um novo peptídeo aniônico, denominado otacidina, foi isolado das secreções cutâneas de três anuros brasileiros Osteocephalus taurinus. O sequenciamento de novo dos aminoácidos foi realizado por meio de análises por espectrometria de massas por tempo de vôo acoplada à ionização dessortiva de matriz por laser (MALDI-TOF-MS/MS). O potencial antimicrobiano deste novo metabólito foi avaliado contra cepas de Escherichia coli e Pseudomonas aeruginosa, sendo observadas concentrações inibitórias mínimas (MICs) de 37,4 e 78,4 mM, respectivamente. Este trabalho é apenas o segundo relato de um peptídeo aniônico com propriedades antimicrobianas, isolado da pele de um anfíbio.21332136Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
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