Reproducibility Analysis Of The Stability And Treatment Of Vertebral Metastatic Lesions

Objectives: To investigate the reproducibility among spine surgeons in defining the treatment of vertebral metastatic lesions, taking into account the mechanical stability of injuries. Methods: Twenty cases of isolated vertebral metastatic lesions were presented to ten experts. Their opinion was then asked about the stability of the lesion, as well as their treatment option. Results: The interobserver Kappa coefficient obtained both for stability analysis as to the decision of the treatment was poor (0.334 and 0.248, respectively). Conclusions: Poor interobserver reproducibility was observed in deciding the treatment of vertebral metastatic lesions when considering the stability of the lesions.133232234American Cancer Society, (2007) Cancer Facts and Figures, , Atlanta: American Cancer SocietyNewman, C.B., Keshavarzi, S., Aryan, H.E., En bloc sacrectomy and reconstruction technique modification for pelvic fixation (2009) Surg Neurol, 72 (6), pp. 752-756Harrington, K.D., Current concepts review: Metastatic disease of the spine (1986) J Bone Joint Surg Am, 68 (7), pp. 1110-1115Gasbarrini, A., Cappuccio, M., Mirabile, L., Bandiera, S., Terzi, S., Barbanti Bròdano, G., Spinal metastases: Treatment evaluation algorithm (2004) Eur Rev Med Pharmacol Sci, 8 (6), pp. 265-274Bilsky, M., Smith, M., Surgical approach to epidural spinal cord compression (2006) Hematol Oncol Clin North Am, 20 (6), pp. 1307-1317Weber, M.H., Burch, S., Buckley, J., Schmidt, M.H., Fehlings, M.G., Vrionis, F.D., Instability and impending instability of the thoracolumbar spine in patients with spinal metastases: A systematic review (2011) Int J Oncol, 38 (1), pp. 5-12Tokuhashi, Y., Kawano, H., Ohsaka, S., Matsuzaki, H., Toriyama, S., A scoring system for preoperative evaluation of the prognosis of metastatic spine tumor (a preliminary report) (1989) Nihon Seikeigeka Gakkai Zasshi, 63 (5), pp. 482-489Tokuhashi, Y., Matsuzaki, H., Toriyama, S., Kawano, H., Ohsaka, S., Scoring system for the preoperative evaluation of metastatic spine tumor prognosis (1990) Spine (Phila Pa 1976), 15 (11), pp. 1110-1113Tomita, K., Kawahara, N., Baba, H., Tsuchiya, H., Fujita, T., Toribatake, Y., Total em bloc spondylectomy. A new surgical technique for primary malignant vertebral tumors (1997) Spine (Phila Pa 1976), 22 (3), pp. 324-333Tomita, K., Kawahara, N., Kobayashi, T., Yoshida, A., Murakami, H., Akamaru, T., Surgical strategy for spinal metastases (2001) Spine (Phila Pa 1976), 26 (3), pp. 298-306Fisher, C.G., DiPaola, C.P., Ryken, T.C., Bilsky, M.H., Shaffrey, C.I., Berven, S.H., A novel classification system for spinal instability in neoplastic disease: An evidence-based approach and expert consensus from the Spine Oncology Study Group (2010) Spine (Phila Pa 1976), 35 (22), pp. E1221-E1229Fourney, D.R., Frangou, E.M., Ryken, T.C., Dipaola, C.P., Shaffrey, C.I., Berven, S.H., Spinal instability neoplastic score: An analysis of reliability and validity from the spine oncology study group (2011) J Clin Oncol, 29 (22), pp. 3072-307

New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat

Septoria tritici blotch (STB), caused by the ascomycete Mycosphaerella graminicola, is one of the most devastating foliar diseases of wheat. We screened five synthetic hexaploid wheats (SHs), 13 wheat varieties that represent the differential set of cultivars and two susceptible checks with a global set of 20 isolates and discovered exceptionally broad STB resistance in SHs. Subsequent development and analyses of recombinant inbred lines (RILs) from a cross between the SH M3 and the highly susceptible bread wheat cv. Kulm revealed two novel resistance loci on chromosomes 3D and 5A. The 3D resistance was expressed in the seedling and adult plant stages, and it controlled necrosis (N) and pycnidia (P) development as well as the latency periods of these parameters. This locus, which is closely linked to the microsatellite marker Xgwm494, was tentatively designated Stb16q and explained from 41 to 71% of the phenotypic variation at seedling stage and 28–31% in mature plants. The resistance locus on chromosome 5A was specifically expressed in the adult plant stage, associated with SSR marker Xhbg247, explained 12–32% of the variation in disease, was designated Stb17, and is the first unambiguously identified and named QTL for adult plant resistance to M. graminicola. Our results confirm that common wheat progenitors might be a rich source of new Stb resistance genes/QTLs that can be deployed in commercial breeding programs

Assessment Of Interobserver Reproducibility Of A New Scale For Management Guidance In Spinal Metastasis - Sins Score [avaliação Da Reprodutibilidade Interobservadores De Uma Nova Escala Para Orientação Da Conduta Terapêutica Nas Metástases Vertebrais - Escore Sins (spine Instability Neoplastic Score)]

Objective: To evaluate the impact on the management and quantify the inter-observer reproducibility of SINS Score. Also, determine its applicability to our milieu. Methods: A presentation of 20 cases of secondary spine lesions was compiled, which was examined by 10 observers. They defined the stability of each injury and, after the presentation of SINS Score, the same cases were presented to the same observers and again they were asked to determine the stability of the lesion. The collected data were analyzed by the Fleiss' Kappa Calculation and Intra-class Correlation. Results: Inter-observer moderate agreement was obtained by using SINS classification. Conclusions: The classification is applicable to our milieu. There was a change of opinion about the stability of the lesions after the presentation of the classification to the observers.114287289Harrington, K.D., Metastatic disease of the spine (1986) J Bone Joint Surg Am., 68 (7), pp. 1110-1115Tokuhashi, Y., Kawano, H., Ohsaka, S., Matsuzaki, H., Toriyama, S., A scoring system for preoperative evaluation of the prognosis of metastatic spine tumor (a preliminary report) (1989) Nihon Seikeigeka Gakkai Zasshi., 63 (5), pp. 482-489Tokuhashi, Y., Matsuzaki, H., Toriyama, S., Kawano, H., Ohsaka, S., Scoring system for the preoperative evaluation of metastatic spine tumor prognosis (1990) Spine (Phila Pa 1976)., 15 (11), pp. 1110-1113Tomita, K., Kawahara, N., Baba, H., Tsuchiya, H., Fujita, T., Toribatake, Y., Total en bloc spondylectomy. A new surgical technique for primary malignant vertebral tumors (1997) Spine (Phila Pa 1976), 22 (3), pp. 324-333Tomita, K., Kawahara, N., Kobayashi, T., Yoshida, A., Murakami, H., Akamaru, T., Surgical strategy for spinal metastases (2001) Spine (Phila Pa 1976)., 26 (3), pp. 298-306Gasbarrini, A., Li, H., Cappuccio, M., Mirabile, L., Paderni, S., Terzi, S., Efficacy evaluation of a new treatment algorithm for spinal metastases (2010) Spine (Phila Pa 1976)., 35 (15), pp. 1466-1470Fisher, C.G., Dipaola, C.P., Ryken, T.C., Bilsky, M.H., Shaffrey, C.I., Berven, S.H., A novel classification system for spinal instability in neoplastic disease: An evidence-based approach and expert consensus from the Spine Oncology Study Group (2010) Spine (Phila Pa 1976), 35 (22), pp. E1221-E1229Chan, P., Boriani, S., Fourney, D.R., Biagini, R., Dekutoski, M.B., Fehlings, M.G., An assessment of the reliability of the Enneking and Weinstein-Boriani- Biagini classifications for staging of primary spinal tumors by the Spine Oncology Study Group (2009) Spine (Phila Pa 1976)., 34 (4), pp. 384-391Fleiss, J.L., (1986) The Design and Analysis of Clinical Experiments., , New York: Wiley;Toumey, J.W., Metastatic malignancy to the spine (1943) J Bone Joint Surg., 25 (2), pp. 292-395Young, J.M., Funk Jr., F.J., Incidence of tumor metastasis to the lumbar spineA comparative study of roentgenographic changes and gross lesions (1953) J Bone Joint Surg Am., 35 (1), pp. 55-64Harrington, K.D., Current concepts review. Metastaic disease to the spine (1986) J Bone Joint Surg Am, 68 (7), pp. 1110-1115Denis, F., Spinal instability as defined by the three-column spine concept in acute spinal trauma (1984) Clin Orthop Relat Res., (189), pp. 65-76White Iii, A.A., Panjabi, M.M., (1978) Clinical Biomecanic of the Spine., , Philadelphia: JB Lippincott;Galasko, C.S., Norris, H.E., Crank, S., Spinal instability secondary to metastatic câncer: Current concepts review (2000) J Bone Joint Surg Am., 82 (4), pp. 570-576. , Apr

Characterization Of The Yeast Flora Present In Some Turkish High-Sugar Products

In this study, 129 Turkish high-sugar products were examined in terms of their yeast flora and 73 representative strains were isolated. Yeast isolates were identified at species level by using Apilab Plus (bioMerieux, France), a specific computer program developed for ID 32C strips (bioMerieux, France). While one of the isolates could be identified at genus level as Aureobasidium, 66 of them were identified as 21 species belonging to 8 different genera. The distribution of these isolates were as follows: Candida (38), Rhodotorula (8), Zygosaccharomyces (7), Cryptococcus (6), Saccharomyces (3), Debaryomyces (2), Pichia (1) and Torulaspora (1). Approximately 70% of the isolates were found to have the ability to grow on media with 50% (w/w) glucose. Hence, they were characterized as xerotolerant strains. Although Zygosaccharomyces rouxii is known as the most xerotolerant yeast species, only two strains of Z rouxii could be isolated from Turkish high-sugar foods. During identification studies, it was observed that ID 32C test strips should certainly be supported by morphological and physiological tests for obtaining more reliable identification results. If not, closely related yeast species such as anamorph and telemorph forms can not be distinguished.WoSScopu