Heredity of seventeen isozyme loci in cassava (Manihot esculenta Crantz)

L'électrophorèse sur gel d'amidon a été utilisée pour évaluer le polymorphisme isoenzymatique chez deux espèces de manioc. Les extraits bruts ont été obtenus à partir de feuilles et de pollen. Dix enzymes ont été examinés pour leur polymorphisme dans une collection de germplasm de 365 cultivars plus 109 échantillons sauvages, la plupart d'Afrique. L'hérédité de ces allozymes a été examinée en utilisant treize descendances intra et interspécifiques. 17 locus polymorphes ont été trouvés pour les dix systèmes enzymatiques, avec 59 allèles. Tous les marqueurs ont montré une hérédité disomique et trois groupes de liaison ont été identifiés. (Résumé d'auteur

Corseul (Côtes-d’Armor), un quartier de la ville antique

Grâce aux fouilles conduites par H Kerèbel. Fanum Martis, actuel Corseul, se dévoile peu à peu. Sur la réserve archéologique de Monterfil II, un vaste ensemble commercial et artisanal a été mis au jour, sur près de 5000 m2, le long d’un axe majeur du chef-lieu de la cité des Coriosolites. Il a ainsi été possible de suivre, de 15/10 av. n è. au IVe s. ap., l’évolution architecturale et topographique du quartier. Le mobilier recueilli, comptant une grande proportion d’estampilles italiques et d’amphores de Lipari, atteste que la cité fut un important marché où s’échangeaient des produits d’origine méditerranéenne. L’étude des vestiges est étayée par une analyse métrologique, menée par un architecte archéologue, qui a notamment permis la restitution des élévations régies par l’ordre toscan. Si Corseul offre encore un vaste champ d’investigations possibles, la fouille de Monterfil II précise désormais comment furent mis en place, sous le règne d’Auguste, le parcellaire urbain et les emprises des domaines public et privé.Under the direction of H. Kértbel, excavation on the site of Fanum Martis. present-day Corseul, has gradually revealed a vast trading and crafts centre within the archaeological protection area of Monterfil II. Uncovering a surface area of over 5000 m2 along the major axis of the former Coriosolati capital has enabled archaeologists to discern architectural and topographical changes which took place in this quarter from 15/10 BCE to the 4th century CE. The finds, which include a high proportion of Italian potters’ marks and Lipari amphorae, attest to the significance ot the town as a marketplace for items produced throughout the Mediterranean Basin. A metrological analysis of the remains, earned out by an archaeologist trained in architecture, has served as the basis for a reconstruction of elevations governed by the Tuscan order. Although there is still much work to be done in Corseul. the excavation carried out thus far at Monterfil II has shown how the urban layout and public and private domains were established during the reign of Augustus

Production, purification, and quality assessment of borrelial proteins CspZ from Borrelia burgdorferi and FhbA from Borrelia hermsii.

peer reviewedBorrelia, spirochetes transmitted by ticks, are the etiological agents of numerous multisystemic diseases, such as Lyme borreliosis (LB) and tick-borne relapsing fever (TBRF). This study focuses on two surface proteins from two Borrelia subspecies involved in these diseases: CspZ, expressed by Borrelia burgdorferi sensu stricto (also named BbCRASP-2 for complement regulator-acquiring surface protein 2), and the factor H binding A (FhbA), expressed by Borrelia hermsii. Numerous subspecies of Borrelia, including these latter, are able to evade the immune defenses of a variety of potential vertebrate hosts in a number of ways. In this context, previous data suggested that both surface proteins play a role in the immune evasion of both Borrelia subspecies by interacting with key regulators of the alternative pathway of the human complement system, factor H (FH) and FH-like protein 1 (FHL-1). The recombinant proteins, CspZ and FhbA, were expressed in Escherichia coli and purified by one-step metal-affinity chromatography, with yields of 15 and 20 mg or pure protein for 1 L of cultured bacteria, respectively. The purity was evaluated by SDS-PAGE and HPLC and is close to about 95%. The mass of CspZ and FhbA was checked by mass spectrometry (MS). Proper folding of CspZ and FhbA was confirmed by circular dichroism (CD), and their biological activity, namely their interaction with purified FH from human serum (recombinant FH15-20 and recombinant FHL-1), was characterized by SPR. Such a study provides the basis for the biochemical characterization of the studied proteins and their biomolecular interactions which is a necessary prerequisite for the development of new approaches to improve the current diagnosis of LB and TBRF. KEY POINTS: • DLS, CD, SEC-MALS, NMR, HPLC, and MS are tools for protein quality assessment • Borrelia spp. possesses immune evasion mechanisms, including human host complement • CspZ and FhbA interact with high affinity (pM to nM) to human FH and rFHL-1

Search for muon-neutrino emission from GeV and TeV gamma-ray flaring blazars using five years of data of the ANTARES telescope

The ANTARES telescope is well-suited for detecting astrophysical transient neutrino sources as it can observe a full hemisphere of the sky at all times with a high duty cycle. The background due to atmospheric particles can be drastically reduced, and the point-source sensitivity improved, by selecting a narrow time window around possible neutrino production periods. Blazars, being radio-loud active galactic nuclei with their jets pointing almost directly towards the observer, are particularly attractive potential neutrino point sources, since they are among the most likely sources of the very high-energy cosmic rays. Neutrinos and gamma rays may be produced in hadronic interactions with the surrounding medium. Moreover, blazars generally show high time variability in their light curves at different wavelengths and on various time scales. This paper presents a time-dependent analysis applied to a selection of flaring gamma-ray blazars observed by the FERMI/LAT experiment and by TeV Cherenkov telescopes using five years of ANTARES data taken from 2008 to 2012. The results are compatible with fluctuations of the background. Upper limits on the neutrino fluence have been produced and compared to the measured gamma-ray spectral energy distribution.Comment: 27 pages, 16 figure

All-sky Search for High-Energy Neutrinos from Gravitational Wave Event GW170104 with the ANTARES Neutrino Telescope

Advanced LIGO detected a significant gravitational wave signal (GW170104) originating from the coalescence of two black holes during the second observation run on January 4$^{\textrm{th}}$, 2017. An all-sky high-energy neutrino follow-up search has been made using data from the ANTARES neutrino telescope, including both upgoing and downgoing events in two separate analyses. No neutrino candidates were found within $\pm500$ s around the GW event time nor any time clustering of events over an extended time window of $\pm3$ months. The non-detection is used to constrain isotropic-equivalent high-energy neutrino emission from GW170104 to less than $\sim4\times 10^{54}$ erg for a $E^{-2}$ spectrum

The Antares Collaboration : Contributions to the 34th International Cosmic Ray Conference (ICRC 2015, The Hague)

The ANTARES detector, completed in 2008, is the largest neutrino telescope in the Northern hemisphere. Located at a depth of 2.5 km in the Mediterranean Sea, 40 km off the Toulon shore, its main goal is the search for astrophysical high energy neutrinos. In this paper we collect the 21 contributions of the ANTARES collaboration to the 34th International Cosmic Ray Conference (ICRC 2015). The scientific output is very rich and the contributions included in these proceedings cover the main physics results, ranging from steady point sources, diffuse searches, multi-messenger analyses to exotic physics