Reconceptualizing participant vulnerability in Scholarship of Teaching and Learning research: exploring the perspectives of health faculty students in Aotearoa New Zealand

© 2023 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC), https://creativecommons.org/licenses/by-nc/4.0/While the need to protect vulnerable research participants is universal, conceptual challenges with the notion of vulnerability may result in the under or over-protection of participants. Ethics review bodies making assumptions about who is vulnerable and in what circumstance can be viewed as paternalistic if they do not consider participant viewpoints. Our study focuses on participant vulnerability in Scholarship of Teaching and Learning (SoTL) research. We aim to illuminate students’ views on participant vulnerability to contribute to critical analysis of the role and processes of ethics review. Additionally, we aim to highlight the importance of seeking the views of participant communities, especially in research environments beyond ethics review’s medical origins. Thirty-four students from a health-related faculty at a university in Aotearoa New Zealand, participated in five focus groups. Participants discussed factors affecting their potential participation in research drawing upon a series of vignettes based on examples of published SoTL projects. Themes, generated using reflexive thematic analysis, built a participant-informed picture of vulnerability. Findings indicate that students do not generally consider themselves vulnerable and instead consider participation in SoTL research through an agentic lens. Students expect that participation will be voluntary, not negatively impact their grades, and not single them out so that others could judge them. Our study also highlights the value students place on relationships with one another and teaching staff and the implications these have for SoTL research participation and future professional practice. This research challenges research ethics committees to think further about vulnerability in the context of SoTL whilst highlighting the importance of providing opportunities for research participants more broadly to explore and vocalize their views as members of participant communities.Peer reviewe

Illuminating the Role of Reflexivity Within Qualitative Pilot Studies: Experiences From a Scholarship of Teaching and Learning Project

© The Author(s) 2022. This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/).Pilot studies within qualitative inquiry are crucial yet often hidden aspects of research design. In this article, we argue for pilots to have greater visibility. We explore the role of a pilot in providing a foundation for enhancing ethical reflexivity, drawing on a recent pilot study within a tertiary healthcare education setting. The Scholarship of Teaching and Learning (SoTL) presents a unique environment with complex stakeholder relationships. There is a lack of consensus nationally and internationally on whether all SoTL projects require consideration by institutional ethics review bodies. A pilot study offers an opportunity for ethical steerage of a research project, reflecting ethics in practice whilst augmenting any procedural ethics review requirements. We propose that a qualitative pilot study, as a design strategy, can enhance ethical conduct by researchers. Within SoTL specifically, the pilot can provide an opportunity for researchers to demonstrate a commitment to a pedagogy of care spanning the project’s duration, signifying a commitment to enduring teacher-student relationships within the broader learning environment. Beyond tertiary settings, we believe the pilot study, as a space for ethical reflexivity, has applicability to research settings where caring for and being seen to care for the wider participant community is a critical ethical consideration.Peer reviewedFinal Published versio

THE EFFECTS OF EXERCISE ON COGNITIVE FUNCTION, AND BALANCE IN HEALTHY INDIVIDUALS - A PILOT STUDY

Concussion is defined as an injury to the brain caused by forces applied to the head. Approximately 10-20% of concussed individuals develop post-concussion syndrome (PCS), characterized by persistent symptoms beyond 10 days including balance and cognitive deficits. The purpose of this pilot study was to explore the effect of a four-week exercise program on physiological, cognitive, and balance variables in a sample of healthy, physically active individuals to gather preliminary information to apply to a future PCS population. Statistically significant changes in reaction time and balance measures were observed. No changes in heart rate, blood pressure, memory, or visual motor speed were observed. Balance improvements in this healthy sample suggest that future exploration of a similar exercise program in those experiencing PCS may provide valuable information

STATIC BALANCE IN INDIVIDUALS WITH POST-CONCUSSION SYNDROME

A concussion is an injury to the brain caused by linear and/or rotational forces applied to the head. The majority of concussions typically resolve spontaneously within a period of 7-10 days. In 10-20% of individuals, however, the symptoms persist beyond 10 days and are termed post-concussion syndrome (PCS). The purpose of this pilot study was to evaluate the effects of a supervised and structured four-week aerobic and balance exercise program on static balance measures in a sample of individuals with PCS. Statistically significant changes in velocity and length of centre of pressure during balancing tasks were observed in response to the exercise program. These improvements in balance and symptoms suggest that further research into the benefits of exercise based treatment for improving balance deficits associated with PCS is warranted

A systematic review of the current evidence regarding interventions for anxiety, PTSD, sleepiness and fatigue in the law enforcement workplace

Law enforcement is inherently stressful, and police officers are particularly vulnerable to mental and physical disorders. As such, researchers are currently assessing intervention strategies that may combat or manage these psychological, physical and mental issues. To review most recent information regarding anxiety, PTSD, and sleepiness and fatigue and identify the interventions and treatments proposed to overcome work related stressors and associated mental illnesses inflicting law enforcement officers. The EMBASE, OVID MEDLINE and PsycINFO databases were canvassed for articles investigating anxiety, post-traumatic stress disorder, sleepiness, and fatigue. Initial article selections were made based on title, whilst final inclusion was informed by a full critical appraisal with respect to the primary and secondary effects. The systematic search returned 363 records, of which 183 were unique. Following screening, 43 records were included in the final review. The included literature assessed the efficacy of several interventions, and provided a number of recommendations regarding interventions, and policy. Moreover, literature indicates that police officers benefit from interventions targeting work-related stress and potential psychological disorders, if these interventions are continuous. Furthermore, larger controlled studies are required to further elucidate the benefits of psychosocial intervention in law enforcement

A pan-African high-resolution drought index dataset

Droughts in Africa cause severe problems, such as crop failure, food shortages, famine, epidemics and even mass migration. To minimize the effects of drought on water and food security on Africa, a high-resolution drought dataset is essential to establish robust drought hazard probabilities and to assess drought vulnerability considering a multi- and cross-sectional perspective that includes crops, hydrological systems, rangeland and environmental systems. Such assessments are essential for policymakers, their advisors and other stakeholders to respond to the pressing humanitarian issues caused by these environmental hazards. In this study, a high spatial resolution Standardized Precipitation-Evapotranspiration Index (SPEI) drought dataset is presented to support these assessments. We compute historical SPEI data based on Climate Hazards group InfraRed Precipitation with Station data (CHIRPS) precipitation estimates and Global Land Evaporation Amsterdam Model (GLEAM) potential evaporation estimates. The high-resolution SPEI dataset (SPEI-HR) presented here spans from 1981 to 2016 (36 years) with 5 km spatial resolution over the whole of Africa. To facilitate the diagnosis of droughts of different durations, accumulation periods from 1 to 48 months are provided. The quality of the resulting dataset was compared with coarse-resolution SPEI based on Climatic Research Unit (CRU) Time Series (TS) datasets, Normalized Difference Vegetation Index (NDVI) calculated from the Global Inventory Monitoring and Modeling System (GIMMS) project and root zone soil moisture modelled by GLEAM. Agreement found between coarse-resolution SPEI from CRU TS (SPEI-CRU) and the developed SPEI-HR provides confidence in the estimation of temporal and spatial variability of droughts in Africa with SPEI-HR. In addition, agreement of SPEI-HR versus NDVI and root zone soil moisture – with an average correlation coefficient (R) of 0.54 and 0.77, respectively – further implies that SPEI-HR can provide valuable information for the study of drought-related processes and societal impacts at sub-basin and district scales in Africa. The dataset is archived in Centre for Environmental Data Analysis (CEDA) via the following link: https://doi.org/10.5285/bbdfd09a04304158b366777eba0d2aeb (Peng et al., 2019a)

Characterisation of protein isoforms encoded by the Drosophila Glycogen Synthase Kinase 3 gene shaggy

The Drosophila shaggy gene (sgg, GSK-3) encodes multiple protein isoforms with serine/threonine kinase activity and is a key player in diverse developmental signalling pathways. Currently it is unclear whether different Sgg proteoforms are similarly involved in signalling or if different proteoforms have distinct functions. We used CRISPR/Cas9 genome engineering to tag eight different Sgg proteoform classes and determined their localization during embryonic development. We performed proteomic analysis of the two major proteoform classes and generated mutant lines for both of these for transcriptomic and phenotypic analysis. We uncovered distinct tissue-specific localization patterns for all of the tagged proteoforms we examined, most of which have not previously been characterised directly at the protein level, including one proteoform initiating with a non-standard codon. Collectively, this suggests complex developmentally regulated splicing of the sgg primary transcript. Further, affinity purification followed by mass spectrometric analyses indicate a different repertoire of interacting proteins for the two major proteoforms we examined, one with ubiquitous expression (Sgg-PB) and one with nervous system specific expression (Sgg-PA). Specific mutation of these proteoforms shows that Sgg-PB performs the well characterised maternal and zygotic segmentations functions of the sgg locus, while Sgg-PA mutants show adult lifespan and locomotor defects consistent with its nervous system localisation. Our findings provide new insights into the role of GSK-3 proteoforms and intriguing links with the GSK-3α and GSK-3β proteins encoded by independent vertebrate genes. Our analysis suggests that different proteoforms generated by alternative splicing are likely to perform distinct functions

Dysregulation of glucose metabolism is an early event in sporadic Parkinson's disease

AbstractUnlike most other cell types, neurons preferentially metabolize glucose via the pentose phosphate pathway (PPP) to maintain their antioxidant status. Inhibiting the PPP in neuronal cell models causes cell death. In rodents, inhibition of this pathway causes selective dopaminergic cell death leading to motor deficits resembling parkinsonism. Using postmortem human brain tissue, we characterized glucose metabolism via the PPP in sporadic Parkinson's disease (PD), Alzheimer's disease (AD), and controls. AD brains showed increased nicotinamide adenine dinucleotide phosphate (NADPH) production in areas affected by disease. In PD however, increased NADPH production was only seen in the affected areas of late-stage cases. Quantifying PPP NADPH-producing enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase by enzyme-linked immunosorbent assay, showed a reduction in the putamen of early-stage PD and interestingly in the cerebellum of early and late-stage PD. Importantly, there was no decrease in enzyme levels in the cortex, putamen, or cerebellum of AD. Our results suggest that down-regulation of PPP enzymes and a failure to increase antioxidant reserve is an early event in the pathogenesis of sporadic PD