Beyond Coding: Using Critical Computational Literacy to Transform Tech

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Validity of telemetric-derived measures of heart rate variability: a systematic review

Heart rate variability (HRV) is a widely accepted indirect measure of autonomic function with widespread application across many settings. Although traditionally measured from the 'gold standard' criterion electrocardiography (ECG), the development of wireless telemetric heart rate monitors (HRMs) extends the scope of the HRV measurement. However, the validity of telemetric-derived data against the criterion ECG data is unclear. Thus, the purpose of this study was twofold: (a) to systematically review the validity of telemetric HRM devices to detect inter-beat intervals and aberrant beats; and (b) to determine the accuracy of HRV parameters computed from HRM-derived inter-beat interval time series data against criterion ECG-derived data in healthy adults aged 19 to 62 yrs. A systematic review of research evidence was conducted. Four electronic databases were accessed to obtain relevant articles (PubMed, EMBASE, MEDLINE and SPORTDiscus. Articles published in English between 1996 and 2016 were eligible for inclusion. Outcome measures included temporal and power spectral indices (Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology (1996). The review confirmed that modern HRMs (Polar® V800™ and Polar® RS800CX™) accurately detected inter-beat interval time-series data. The HRV parameters computed from the HRM-derived time series data were interchangeable with the ECG-derived data. The accuracy of the automatic in-built manufacturer error detection and the HRV algorithms were not established. Notwithstanding acknowledged limitations (a single reviewer, language bias, and the restricted selection of HRV parameters), we conclude that the modern Polar® HRMs offer a valid useful alternative to the ECG for the acquisition of inter-beat interval time series data, and the HRV parameters computed from Polar® HRM-derived inter-beat interval time series data accurately reflect ECG-derived HRV metrics, when inter-beat interval data are processed and analyzed using identical protocols, validated algorithms and software, particularly under controlled and stable conditions

Early vessel destabilization mediated by Angiopoietin-2 and subsequent vessel maturation via Angiopoietin-1 induce functional neovasculature after ischemia.

We assessed whether Angiopoietin-2 (Ang2), a Tie2 ligand and partial antagonist of Angiopoietin-1 (Ang1), is required for early vessel destabilization during postischemic angiogenesis, when combined with vascular growth factors. In vitro, matrigel co-cultures assessed endothelial-cell tube formation and pericyte recruitment after stimulation of VEGF-A, Apelin (APLN), Ang1 with or without Ang2. In a murine hindlimb ischemia model, adeno-associated virus (rAAV, 3×10(12) virusparticles) transduction of VEGF-A, APLN and Ang1 with or without Ang2 (continuous or early expression d0-3) was performed intramuscularly (d-14). Femoral artery ligation was performed at d0, followed by laser doppler perfusion meassurements (LDI) 7 and 14. At d7 (early timepoint) and d14 (late timepoint), histological analysis of capillary/muscle fiber ratio (CMF-R, PECAM-1) and pericyte/capillary ratio (PC-R, NG2) was performed. In vitro, VEGF-A, APLN and Ang1 induced ring formation, but only APLN and Ang1 recruited pericytes. Ang2 did not affect tube formation by APLN, but reduced pericyte recruitment after APLN or Ang1 overexpression. In vivo, rAAV.VEGF-A did not alter LDI-perfusion at d14, consistent with an impaired PC-R despite a rise in CMF-R. rAAV.APLN improved perfusion at d14, with or without continuous Ang2, increasing CMF-R and PC-R. rAAV.Ang1 improved perfusion at d14, when combined with rAAV.Ang2 (d0-3), accompanied by an increased CMF-R and PC-R. The combination of early vessel destabilization (Ang2 d0-3) and continuous Ang1 overexpression improves hindlimb perfusion, pointing to the importance of early vessel destabilization and subsequent vessel maturation for enhanced therapeutic neovascularization

Prevalence of Substance Use among Lesbian, Gay, and Bisexual High School Students in 2019 vs. 2021

Background: Studies suggest that LGBTQ+ (lesbian, gay, bisexual, transgender, queer/questioning, plus) youth are at increased risk of engaging in alcohol and substance use. The COVID-19 pandemic forced many students to isolate in 2020 and 2021, and the impacts of this have been purported to be detrimental. This study examines the prevalence of alcohol and substance use among lesbian, gay, and bisexual (LGB) U.S. high school students before the pandemic in 2019 versus 2021. Methods: This cross-sectional descriptive study used results from the 2019 Youth Risk Behavior Survey (YRBS) and the 2021 Adolescent Behavior and Experiences Survey (ABES). The 2019 YRBS included 13,677 questionnaires from 136 schools, and the 2021 ABES included 7,705 questionnaires from 128 schools. We selected 15 questions concerning alcohol and substance use common to both surveys and compared results for LGB students in 2019 versus 2021. Results: We found a decrease in prevalence for all 15 alcohol and substance-use-related questions from 2019 to 2021. The most statistically significant differences were observed in E-cigarette use, marijuana use, and obtaining an illegal drug on school property. Conclusions: The results suggest substantial decreases in alcohol and substance use among LGB high school students from 2019 to 2021. These decreases have occurred in the context of the pandemic and reported increases in mental health problems among this population. These outcomes should prompt further research into how social interactions at school (or the lack thereof) among LGBTQ+ youth contribute to patterns of alcohol and substance use

Effect of alternating day and night temperature on short day-induced bud set and subsequent bud burst in long days in Norway spruce

Young seedlings of the conifer Norway spruce exhibit short day (SD)-induced cessation of apical growth and bud set. Although different, constant temperatures under SD are known to modulate timing of bud set and depth of dormancy with development of deeper dormancy under higher compared to lower temperature, systematic studies of effects of alternating day (DT) and night temperatures (NT) are limited. To shed light on this, seedlings of different provenances of Norway spruce were exposed to a wide range of DT-NT combinations during bud development, followed by transfer to forcing conditions of long days (LD) and 18°C, directly or after different periods of chilling. Although no specific effect of alternating DT/NT was found, the results demonstrate that the effects of DT under SD on bud set and subsequent bud break are significantly modified by NT in a complex way. The effects on bud break persisted after chilling. Since time to bud set correlated with the daily mean temperature under SD at DTs of 18 and 21°C, but not a DT of 15°C, time to bud set apparently also depend on the specific DT, implying that the effect of NT depends on the actual DT. Although higher temperature under SD generally results in later bud break after transfer to forcing conditions, the fastest bud flush was observed at intermediate NTs. This might be due to a bud break-hastening chilling effect of intermediate compared to higher temperatures, and delayed bud development to a stage where bud burst can occur, under lower temperatures. Also, time to bud burst in un-chilled seedlings decreased with increasing SD-duration, suggesting that bud development must reach a certain stage before the processes leading to bud burst are initiated. The present results also indicate that low temperature during bud development had a larger effect on the most southern compared to the most northern provenance studied. Decreasing time to bud burst was observed with increasing northern latitude of origin in un-chilled as well as chilled plants. In conclusion, being a highly temperature-dependent process, bud development is strongly delayed by low temperature, and the effects of DT is significantly modified by NT in a complex manner

Osteopontin ablation ameliorates muscular dystrophy by shifting macrophages to a pro-regenerative phenotype.

In the degenerative disease Duchenne muscular dystrophy, inflammatory cells enter muscles in response to repetitive muscle damage. Immune factors are required for muscle regeneration, but chronic inflammation creates a profibrotic milieu that exacerbates disease progression. Osteopontin (OPN) is an immunomodulator highly expressed in dystrophic muscles. Ablation of OPN correlates with reduced fibrosis and improved muscle strength as well as reduced natural killer T (NKT) cell counts. Here, we demonstrate that the improved dystrophic phenotype observed with OPN ablation does not result from reductions in NKT cells. OPN ablation skews macrophage polarization toward a pro-regenerative phenotype by reducing M1 and M2a and increasing M2c subsets. These changes are associated with increased expression of pro-regenerative factors insulin-like growth factor 1, leukemia inhibitory factor, and urokinase-type plasminogen activator. Furthermore, altered macrophage polarization correlated with increases in muscle weight and muscle fiber diameter, resulting in long-term improvements in muscle strength and function in mdx mice. These findings suggest that OPN ablation promotes muscle repair via macrophage secretion of pro-myogenic growth factors

Trends in Key Performance Indicators Among Colleges Participating in a Technology-Mediated Advising Reform Initiative

In 2015, 26 two- and four-year institutions received three-year grants to implement reforms consistent with the Integrated Planning and Advising for Student Success (iPASS) initiative. Grantees committed to launching or enhancing existing technologies and undertake related organizational changes that would enable them to provide more effective advising and support to all students. CCRC was engaged as a research partner to document the iPASS colleges’ implementation efforts and outcomes. CCRC analyzed key performance indicators (KPIs)—including student retention from the first to the second semester and first-term grade point average—using administrative data collected from all 26 participating institutions. This report describes trends in KPIs, aggregated by college sector and cohort, in the participating colleges from 2011 to 2017—from the period before the initiative started until about two years after the grants were awarded. Because iPASS reforms cannot easily be isolated from other reforms the colleges were carrying out, it is difficult to directly associate the modest changes observed over time in KPIs. This report also includes college scores on an iPASS development index, a weighted measure CCRC researchers created to gain insights into the level of adoption of iPASS technologies and practices across the 26 grantee institutions as of fall 2017, when colleges provided answers to an institutional survey about their efforts. The authors find that while no institution had fully implemented iPASS, at most institutions, substantial progress was being made