Capteur optofluidique à base de microrésonateur en polymère pour la détection d'hybridation d'ADN en vue d'applications biomédicales

International audienceCet article présente un capteur optofluidique à base de microrésonateur en polymère et en couplage vertical, incorporé dans un circuit microfluidique, également en polymère, pour une détection précise, en temps réel et spécifique de brins d'ADN en solution par hybridation d'ADN. Le capteur présente une détection limite de 160 nmol/L

Osteopoikilosis: Report of a patient with associated complex knee injuries and literature review

ABSTRACTIOsteopoikilosis (OPK) is a rare autosomal dominant hereditary disease with a prevalence estimated at 1/50000. Most of the time asymptomatic and benign, it can be misdiagnosed as other osteoblastic bone diseases (such as bone metastases) and lead to unnecessary stress and costly investigation. We report a 15-year-old boy with a complicated knee injury and associated OPK as well as a literature review.KEYWORDS Osteopoikilosis, Multiple sclerotic lesions, sclerosing bone dysplasiainfo:eu-repo/semantics/publishe

Evaluation des patients opérés d'une fracture fémorale proximale dans le cadre du programme Fragility Fracture durant la période de confinement belge

Introduction :The management of SARS-CoV-2 infection has completely reformed the medical sector. Between March 14 and May 17 2020, only urgent surgical operations remained, including the management of proximal femoral fractures. The aim of our study is to best qualify this population of surgical patients by evaluating some clinical and epidemiological characteristics and assessing the infection rate. Material and Methods :This is a retrospective, descriptive and mono-centric cohort study, which identifies all Fragility Fracture program patients who had surgery for a proximal femoral fracture between March 14, 2020 and May 17, 2020 in the CHU Tivoli. Overall we defined the population and analyzed clinical and biological characteristics of patients suspected of or suffering from COVID-19, but also patients who developed the infection during hospitalization, compared to patients considered healthy. Finally, we described our deceased cases. Results :We treated 18 proximal femoral fractures during this period. Eight patients were operated following the COVID-19 precautions. Six patients tested positive, 3 on admission and 3 who become infected during their time in hospital. There were no physiological or biological differences between patients on admission. The mortality rate for positive patients was 33%. Conclusion :Unlike other studies, our incidence of proximal femoral fractures did not decrease during this time. These fractures occur in patients with early stage COVID-19, however the mortality rate remains high.SCOPUS: ar.jDecretOANoAutActifinfo:eu-repo/semantics/publishe

Normes d’association sémantique et phonologique chez l’enfant au développement typique de 4 à 9 ans

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Arthrite septìque du genou causée par un germe inhabituel: à proposďun cas

Introduction: Osteo-articular infections witPi Streptococcus canis are rare. To our l<nowledge, only one case of septic osteoartiiritis witii S. canis is reported in the scientific literature. Clinical case: We report the case of a 55-year-old patient who presented with left gonalgia without trauma. He was bitten on the leg by his dog a few days earlier. The biological inflammatory syndrome was moderately significant. The complementary explorations allowed to set the diagnosis of septic osteoarthritis. Discussion: Streptococcus canis is mostly known in the veterinary area and only represents 1 % of the streptococcal infections in humans. This germ is mainly responsible of soft tissue infections but can also affect other areas. Conclusion: Septic arthritis with S. canis is a rare condition and is a therapeutic and diagnostic emergency. The puncture is essential before starting the antibiotherapy.SCOPUS: ar.jDecretOANoAutActifinfo:eu-repo/semantics/publishe

Semantic and phonological association norms in French speaking children from ages 4 to 9 years

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Psychometric properties of the Cumulated Ambulation Score French translation

AbstractObjective:To examine measurement properties (inter-rater reliability, agreement, validity, and responsiveness) of the Cumulated Ambulation Score French translation in patients with hip fracture.Design:Methodological study.Setting:A 20-bed orthopedic unit and 20-bed geriatric unit.Subjects:About 140 consecutive patients with a mean (SD) age of 83 (12) years.Interventions:The English version of the Cumulated Ambulation Score used to evaluate basic mobility was translated into French following international guidelines. Two raters independently assessed all patients on postoperative days one, two, three, and 30 after a hip fracture surgery. Relative and absolute inter-rater reliability and responsiveness (effect size for improvement from postoperative day two to 30) were evaluated. Convergent validity was analyzed by Spearman’s correlation coefficient comparing the Cumulated Ambulation Score with two other measures on postoperative day two and 30.Main measure:Cumulated Ambulation Score.Results:The weighted Kappa value ranged from 0.89 to 1.0. The standard error of measurement and the smallest real difference of the Cumulated Ambulation Score ranged, respectively, from 0.12 to 0.23 and from 0.32 to 0.6 points, while the effect size reached 1.03 (95% CI 0.87–1.26). There was a strong positive correlation comparing the Cumulated Ambulation Score with the French Tinetti Assessment Tool (r ⩾ 0.83) and the French Mini Motor Test (r ⩾ 0.79).Conclusions:Our findings indicate that the Cumulated Ambulation Score’s French version is a reliable and valid tool to assess patients’ basic mobility with hip fractures.info:eu-repo/semantics/publishe

Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species

International audienceThe accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor based on a polymer-based microresonator which shows a quick response time, a low detection limit and good sensitivity. The device is composed of a micro-racetrack waveguide vertically coupled to a bus waveguide and embedded within a microfluidic circuit. The spectral response of the microresonator, in air or immersed in deionised water, shows quality factors up to 72,900 and contrasts up to 0.9. The concentration of DNA strands in water is related to the spectral shift of the microresonator transmission function, as measured at the inflection points of resonance peaks in order to optimize the signal-over-noise ratio. After functionalization by a DNA probe strand on the surface of the microresonator, a specific and real time measurement of the complementary DNA strands in the solution is realized. Additionally, we have inferred the dissociation constant value of the binding equilibrium of the two complementary DNA strands and evidenced a sensitivity of 16.0 pm/µM and a detection limit of 121 nM

Anderson-Type Polyoxometalates Functionalized by Tetrathiafulvalene Groups Synthesis, Electrochemical Studies, and NLO Properties

International audienceThree polyoxometalates (POMs) functionalized by tetrathiafulvalene (TTF) molecules have been synthesized by a coupling reaction between the Anderson-type POMs [MnMoO{(OCH)CNH}] or [AlMoO(OH){(OCH)CNH}] and the TTF carboxylic acid derivative (MeS)TTF(S-CH-COH). The monofunctionalized TTF-AlMo POM contains one TTF group covalently grafted on an Al Anderson platform. The symmetrical TTF-MnMo-TTF POM possesses two TTF groups grafted on each side of a Mn Anderson derivative while the asymmetrical TTF-MnMo-SP POM contains a TTF and a spiropyran groups. These three trianionic species have been characterized by elemental analysis, H and C NMR, FT-IR spectroscopy, ESI-MS spectrometry, and single-crystal X-ray diffraction (for TTF-MnMo-TTF). In the solid state, the grafted TTF molecules of TTF-MnMo-TTF POMs interact via S···S and π···π interactions and form chains. The electrochemical properties of the complexes reflect the contributions of both the inorganic POM and the TTF moieties. Despite adsorption of the oxidized hybrid species on the Pt grid working electrode, UV-vis-NIR spectroelectrochemical investigations evidence peaks characteristic of the oxidation of the TTF units. Finally, hyper-Rayleigh scattering (HRS) measurements show that the three novel TTF derivatives exhibit β values between 20 and 37 × 10 esu. Moreover it is observed that the oxidation of the TTF moieties by Fe ions increases the NLO response. These values are in the order of magnitude of that found for the well-known 4-dimethylamino- N-methyl-4-stilbazolium (DAS) cation (β = 60 × 10 esu)