A breakthrough on Amanita phalloides poisoning: an effective antidotal effect by polymyxin B

Amanita phalloides is responsible for more than 90 % of mushroom-related fatalities, and no effective antidote is available. a-Amanitin, the main toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and kidney failure. In silico studies included docking and molecular dynamics simulation coupled to molecular mechanics with generalized Born and surface area method energy decomposition on RNAP II. They were performed with a clinical drug that shares chemical similarities to a-amanitin, polymyxin B. The results show that polymyxin B potentially binds to RNAP II in the same interface of a-amanitin, preventing the toxin from binding to RNAP II. In vivo, the inhibition of the mRNA transcripts elicited by a-amanitin was efficiently reverted by polymyxin B in the kidneys. Moreover, polymyxin B significantly decreased the hepatic and renal a-amanitin-induced injury as seen by the histology and hepatic aminotransferases plasma data. In the survival assay, all animals exposed to a-amanitin died within 5 days, whereas 50 % survived up to 30 days when polymyxin B was administered 4, 8, and 12 h post-a-amanitin. Moreover, a single dose of polymyxin B administered concomitantly with a-amanitin was able to guarantee 100 % survival. Polymyxin B protects RNAP II from inactivation leading to an effective prevention of organ damage and increasing survival in a-amanitin-treated animals. The present use of clinically relevant concentrations of an already human-use-approved drug prompts the use of polymyxin B as an antidote for A. phalloides poisoning in humans.Juliana Garcia, Vera Marisa Costa, Ricardo Dinis-Oliveira and Ricardo Silvestre thank FCT-Foundation for Science and Technology-for their PhD grant (SFRH/BD/74979/2010), Post-doc grants (SFRH/BPD/63746/2009 and SFRH/BPD/110001/2015) and Investigator grants (IF/01147/2013) and (IF/00021/2014), respectively. This work was supported by the Fundacao para a Ciencia e Tecnologia (FCT) - project PTDC/DTPFTO/4973/2014 - and the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundacao para a Ciencia e Tecnologia) through project Pest-C/EQB/LA0006/2013

Monocytes non classiques (caractérisation immunologique et intérêt en pathologie)

BORDEAUX2-BU Santé (330632101) / SudocSudocFranceF

Deep oceanic submarine fieldwork with undergraduate students: an immersive experience with the Minerve software

International audienceWe present the content and scripting of an active tectonic lab session conceived for third-year undergraduate students studying Earth sciences at Observatoire des Sciences de l'Univers in Lyon. This session is based on a research project conducted on the submarine Roseau active fault in the Lesser Antilles. The fault morphology is particularly interesting to map as this structure in the deep ocean is preserved from weathering. Thus, high-resolution models computed from remotely operated vehicle (ROV) videos provide exceptional educational material to link fault morphology and coseismic displacement. This class includes mapping exercises on geographical information systems and virtual fieldwork to provide basic understanding of active tectonics and active fault morphology in particular. The work has been conducted either in a full remote configuration via 3D online models or in virtual reality (VR) in a dedicated room using the Minerve software. During the VR sessions, students were either alone in the VR environment or participated as a group that included the instructor (physically in the classroom or remotely from another location), which is to our knowledge one of the first attempts of this kind in France. We discuss the efficiency of virtual fieldwork using VR based on feedback from teachers and students. We conclude that VR is a promising tool to learn observational skills in Earth sciences, subject to certain improvements that should be possible in the years to come

A Tumour and Liver Automatic Segmentation (ATLAS) Dataset on Contrast-Enhanced Magnetic Resonance Imaging for Hepatocellular Carcinoma

International audienceLiver cancer is the sixth most common cancer in the world and the fourth leading cause of cancer mortality. In unresectable liver cancers, especially hepatocellular carcinoma (HCC), transarterial radioembolisation (TARE) can be considered for treatment. TARE treatment involves a contrastenhanced magnetic resonance imaging (CE-MRI) exam performed beforehand to delineate the liver and tumour(s) in order to perform dosimetry calculation. Due to the significant amount of time and expertise required to perform the delineation process, there is a strong need for automation. Unfortunately, the lack of publicly available CE-MRI datasets with liver tumour annotations has hindered the development of fully automatic solutions for liver and tumour segmentation. The "Tumour and Liver Automatic Segmentation" (ATLAS) dataset that we present consists of 90 liver-focused CE-MRI covering the entire liver of 90 patients with unresectable HCC, along with 90 liver and liver tumour segmentation masks. To the best of our knowledge, the ATLAS dataset is the first public dataset providing CE-MRI of HCC with annotations. The public availability of this dataset should greatly facilitate the development of automated tools designed to optimise the delineation process, which is essential for treatment planning in liver cancer patients

Retour dans la patrie : marche créée par Mme Amiati à la Scala [illustration couleurs Ch. Clérice (Charles Clérice (1855-1912)]

Retour dans la patrie : marche créée par Mme Amiati à la Scala ; (dédicace) “A Madame Peyrali de l’Eden-Concert” ; illustration couleurs Ch. Clérice [Charles Clérice] ; paroles Félix Mortreuil & Eugène Leclerc ; musique R. Goublier [Renaud Goublier signature de jeunesse de Gustave Goublier (1856-1926)] ; sans mention d’éditeur sur le recto ; [intérieur : mentions identiques dédicace, artiste et lieux ; précision éditeur : E. Meuriot, 25 boulevard de Strasbourg ; sans date, sans cotage ; L. Parent gravure ; imprimerie Joly] ; verso vierge ; incipit “Entendez-vous le tambour qui résonne” ; datation (titre) 1885, par dépôt BNF titre édité par Egrot ; datation exemplaire (Meuriot) ≥1885 après achat du fonds Egrot par Meuriot. Une chanson du même titre est mentionnée comme “création d’Amiati” dans la presse spécialisée en 1885 (numérisée et consultable sur Gallica)