Differentiation and its Effects on the Development of Student Study Skills

Differentiation is used by educators to better meet the needs of all students; yet, differentiation has been found most effective when students are a part of the discussion regarding their individual needs, learning styles and preferences in the classroom (Tomlinson, 2008). Making students aware of differentiation and choices in the classroom raises student self-awareness and the development of skills overtime. During my student teaching, I implemented differentiated review activities and began a dialogue with students about their learning preferences, studying habits, and experience with taking social studies exams. Over the course of the semester, students began choosing study materials that aligned with how each of them learned best. Through self-reflection activities, students better understood why their study habits and choices were effective or not. I utilized these self-reflection activities, field notes, and a summative survey as data sources to determine how differentiation impacted the development of student study skills throughout the semester. Students were enthusiastic about receiving guidance and information about effective studying; students became aware of the correlation between effective study habits and test performance throughout the semester

Group simulation for “authentic” assessment in a maternal-child lecture course

The purpose of this pilot study was to explore student perceptions and outcomes surrounding the use of a labor and delivery simulation as a midterm exam in a maternal-newborn lecture course.  An exploratory case study design was used to gain a holistic view of the simulation experience.  Data from focus groups, written debriefings, simulation scoring rubrics, student course evaluations, and other course exams were analyzed using Stake’s case study method.  Qualitative analysis revealed four themes: confidence, fairness, reliability, and team effort.  Students were able to accurately grade the performance of their group as a whole and complete a group self-debriefing, but quantitative analysis showed that the group scores were significantly higher than other individual course grades. The findings suggested that the group simulation was an authentic assessment of teamwork, but not individual performance. Future research is needed to determine what role simulation and collaborative testing should play in pre-licensure education.

Transcriptional profiling predicts running promotes cerebrovascular remodeling in young but not midlife mice.

BACKGROUND: The incidence of dementia and cognitive decline is increasing with no therapy or cure. One of the reasons treatment remains elusive is because there are various pathologies that contribute to age-related cognitive decline. Specifically, with Alzheimer\u27s disease, targeting to reduce amyloid beta plaques and phosphorylated tau aggregates in clinical trials has not yielded results to slow symptomology, suggesting a new approach is needed. Interestingly, exercise has been proposed as a potential therapeutic intervention to improve brain health and reduce the risk for dementia, however the benefits throughout aging are not well understood. RESULTS: To better understand the effects of exercise, we preformed transcriptional profiling on young (1-2 months) and midlife (12 months) C57BL/6 J (B6) male mice after 12 weeks of voluntary running. Data was compared to age-matched sedentary controls. Interestingly, the midlife running group naturally broke into two cohorts based on distance ran - either running a lot and more intensely (high runners) or running less and less intensely (low runners). Midlife high runners had lower LDL cholesterol as well as lower adiposity (%fat) compared to sedentary, than midlife low runners compared to sedentary suggesting more intense running lowered systemic markers of risk for age-related diseases including dementias. Differential gene analysis of transcriptional profiles generated from the cortex and hippocampus showed thousands of differentially expressed (DE) genes when comparing young runners to sedentary controls. However, only a few hundred genes were DE comparing either midlife high runners or midlife low runners to midlife sedentary controls. This indicates that, in our study, the effects of running are reduced through aging. Gene set enrichment analyses identified enrichment of genes involved in extracellular matrix (ECM), vascular remodeling and angiogenesis in young runners but not midlife runners. These genes are known to be expressed in multiple vascular-related cell types including astrocytes, endothelial cells, pericytes and smooth muscle cells. CONCLUSIONS: Taken together these results suggest running may best serve as a preventative measure to reduce risk for cerebrovascular decline. Ultimately, this work shows that exercise may be more effective to prevent dementia if introduced at younger ages

Emotion and Virality of Food Safety Risk Communication Messages on Social Media

This study investigates how the emotional tone of food safety risk communication messages predicts message virality on social media. Through a professional Internet content tracking service, we gathered news articles written about the 2018 romaine lettuce recall published online between October 30th and November 29th, 2018. We retrieved the number of times each article was shared on Twitter and Pinterest, and the number of engagements (shares, likes, and comments) for each article on Facebook and Reddit. We randomly selected 10% of the articles (n = 377) and characterized the emotional tone of each article using machine learning, including emotional characteristics such as discrete emotions, emotional valence, arousal, and dominance. Conveying negative valence, low arousal, and high dominance, as well as anger and sadness emotions were associated with greater virality of articles on social media. Implications of these findings for risk communication in the age of social media are discussed

Relationship Between Neck Circumference and Cardiometabolic Parameters in HIV-Infected and non–HIV-Infected Adults

OBJECTIVE: Upper body fat is associated with increased cardiometabolic risk. More recently, neck circumference (NC) and/or neck fat have been associated with hyperlipidemia, impaired glucose homeostasis, and hypertension. The objective of this study was to determine whether this relationship is evident in HIV-infected individuals, who often exhibit changes in relative fat distribution, and to determine whether NC is independently associated with carotid intima-media thickness (cIMT) in HIV and non–HIV-infected patients. RESEARCH DESIGN AND METHODS: Body composition, including anthropometrics, visceral adipose tissue assessment by CT, and metabolic parameters, including lipids, cIMT, and oral glucose tolerance test, were measured in 174 men and women with HIV infection and 154 non–HIV-infected subjects. NC was measured in triplicate inferior to the laryngeal prominence. RESULTS: In univariate analysis, NC was significantly and positively related to blood pressure, hemoglobin A1c, glucose, and insulin and significantly and negatively related to HDL cholesterol in HIV-infected individuals and HIV-negative control subjects. NC was significantly associated with cIMT in univariate regression analysis among HIV-infected (r = 0.21, P = 0.006) and non–HIV-infected (r = 0.31, P = 0.0001) patients. This relationship remained significant among non–HIV-infected patients (R2 = 0.45, P < 0.001) but not HIV-infected patients in multivariate modeling controlling for age, sex, race, smoking hypertension, glucose, and lipids. CONCLUSIONS: Among both HIV and non–HIV-infected patients, increased NC is strongly associated with decreased HDL and impaired glucose homeostasis. Among non–HIV-infected subjects, NC also predicts increased cIMT when controlling for traditional risk factors

DISSEMINATION OF GEOLOGICAL INFORMATION IN AVOIDING GEOTECHNICAL RISKS ASSOCIATED WITH TUNNEL CONSTRUCTION: LESSONS LEARNED FROM DEEP VOIDS IN MARBLE IN LOWER MANHATTAN, NEW YORK CITY

Test boring associated with the recently accomplished City Water Tunnel # 3 in Manhattan, New York City revealed moderately to highly weathered marble with insoluble silicate residues composed mostly of phlogopite, chlorite, and tremolite. Apparent control on the weatherability of the marble was in response to original mineral constituents dominating this lithology. Encountered marble samples ranged between pure calcitic to mixed dolomitic/siliceous types. Fresh marble samples collected from adjacent boring locations revealed characteristic geochemical data: Lime (25-45%), Silica (4-7%), Alumina (1-3%), MgO (5-20%), and LOI (35-42%); compressive strength of unweathered marble varied between 2000-3000 Kg/cm2. Relict foliations with schistose in nature were also noted in some marbles. Geotechnical characteristics such as sudden decrease in N-value (blow counts), faster drilling rate, and poor RQD (Rock Quality Designation) associated with this weathered zone also suggested incompetent and unconsolidated rock debris. Both physical and geotechnical attributes of the marble indicated the existence of dissolution-controlled subsurface conditions. Although the probability is low, deep voids in the marble in Lower Manhattan could cause problems if the tunnel boring operation intersects them. Usually, solution voids in carbonates do not occur at such depth, unless there is the presence of intricate joint or fracture systems, which continue deep within the subsurface rocks. However, faulting, in and around Canal Street, could create just such a situation. Possible occurrences of complex and perhaps interconnected fractures, enlarged by solution within the marble, may create an easy passageway for water flow. Moreover, it appears that such a void was encountered by a test boring on Bowery Street just south of Canal Street at a depth of about 200 feet. If a water filled void, which is part of an interconnected fracture system, is encountered during the tunnel boring, then water could enter the tunnel at a rate that would be difficult to control. From the test boring data, it appears that the present, proposed tunnel alignment is well west of the major occurrence of the marble. Nevertheless, it would be prudent to be alert to signs of excessive water entering the tunnel, particularly during the probe drilling operations

Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots.

Control over cell growth by mobile regulators underlies much of eukaryotic morphogenesis. In plant roots, cell division and elongation are separated into distinct longitudinal zones and both division and elongation are influenced by the growth regulatory hormone gibberellin (GA). Previously, a multicellular mathematical model predicted a GA maximum at the border of the meristematic and elongation zones. However, GA in roots was recently measured using a genetically encoded fluorescent biosensor, nlsGPS1, and found to be low in the meristematic zone grading to a maximum at the end of the elongation zone. Furthermore, the accumulation rate of exogenous GA was also found to be higher in the elongation zone. It was still unknown which biochemical activities were responsible for these mobile small molecule gradients and whether the spatiotemporal correlation between GA levels and cell length is important for root cell division and elongation patterns. Using a mathematical modeling approach in combination with high-resolution GA measurements in vivo, we now show how differentials in several biosynthetic enzyme steps contribute to the endogenous GA gradient and how differential cellular permeability contributes to an accumulation gradient of exogenous GA. We also analyzed the effects of altered GA distribution in roots and did not find significant phenotypes resulting from increased GA levels or signaling. We did find a substantial temporal delay between complementation of GA distribution and cell division and elongation phenotypes in a GA deficient mutant. Together, our results provide models of how GA gradients are directed and in turn direct root growth

VUV Photoionization Cross Sections of HO_2, H_2O_2, and H_2CO

The absolute vacuum ultraviolet (VUV) photoionization spectra of the hydroperoxyl radical (HO_2), hydrogen peroxide (H_2O_2), and formaldehyde (H_2CO) have been measured from their first ionization thresholds to 12.008 eV. HO_2, H_2O_2, and H_2CO were generated from the oxidation of methanol initiated by pulsed-laser-photolysis of Cl_2 in a low-pressure slow flow reactor. Reactants, intermediates, and products were detected by time-resolved multiplexed synchrotron photoionization mass spectrometry. Absolute concentrations were obtained from the time-dependent photoion signals by modeling the kinetics of the methanol oxidation chemistry. Photoionization cross sections were determined at several photon energies relative to the cross section of methanol, which was in turn determined relative to that of propene. These measurements were used to place relative photoionization spectra of HO_2, H_2O_2, and H_2CO on an absolute scale, resulting in absolute photoionization spectra

Identification of Critical Paraoxonase 1 Residues Involved in High Density Lipoprotein Interaction

Paraoxonase 1 (PON1) is a high density lipoprotein (HDL)-associated protein with atherosclerosis-protective and systemic anti-oxidant functions. We recently showed that PON1, myeloperoxidase, and HDL bind to one another in vivo forming a functional ternary complex (Huang, Y., Wu, Z., Riwanto, M., Gao, S., Levison, B. S., Gu, X., Fu, X., Wagner, M. A., Besler, C., Gerstenecker, G., Zhang, R., Li, X. M., Didonato, A. J., Gogonea, V., Tang, W. H., et al. (2013) J. Clin. Invest. 123, 3815–3828). However, specific residues on PON1 involved in the HDL-PON1 interaction remain unclear. Unambiguous identification of protein residues involved in docking interactions to lipid surfaces poses considerable methodological challenges. Here we describe a new strategy that uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when incorporated into HDL, was used to identify amino acid residues on PON1 that directly interact with the lipoprotein phospholipid surface. Several specific PON1 residues (Leu-9, Tyr-185, and Tyr-293) were identified through covalent cross-links with the lipid probes using affinity isolation coupled to liquid chromatography with on-line tandem mass spectrometry. Based upon the crystal structure for PON1, the identified residues are all localized in relatively close proximity on the surface of PON1, defining a domain that binds to the HDL lipid surface. Site-specific mutagenesis of the identified PON1 residues (Leu-9, Tyr-185, and Tyr-293), coupled with functional studies, reveals their importance in PON1 binding to HDL and both PON1 catalytic activity and stability. Specifically, the residues identified on PON1 provide important structural insights into the PON1-HDL interaction. More generally, the new photoactivatable and affinity-tagged lipid probe developed herein should prove to be a valuable tool for identifying contact sites supporting protein interactions with lipid interfaces such as found on cell membranes or lipoproteins