Ceramic identity contributes to mechanical properties and osteoblast behavior on macroporous composite scaffolds.

Implants formed of metals, bioceramics, or polymers may provide an alternative to autografts for treating large bone defects. However, limitations to each material motivate the examination of composites to capitalize on the beneficial aspects of individual components and to address the need for conferring bioactive behavior to the polymer matrix. We hypothesized that the inclusion of different bioceramics in a ceramic-polymer composite would alter the physical properties of the implant and the cellular osteogenic response. To test this, composite scaffolds formed from poly(lactide-co-glycolide) (PLG) and either hydroxyapatite (HA), β-tricalcium phosphate (TCP), or bioactive glass (Bioglass 45S®, BG) were fabricated, and the physical properties of each scaffold were examined. We quantified cell proliferation by DNA content, osteogenic response of human osteoblasts (NHOsts) to composite scaffolds by alkaline phosphatase (ALP) activity, and changes in gene expression by qPCR. Compared to BG-PLG scaffolds, HA-PLG and TCP-PLG composite scaffolds possessed greater compressive moduli. NHOsts on BG-PLG substrates exhibited higher ALP activity than those on control, HA-, or TCP-PLG scaffolds after 21 days, and cells on composites exhibited a 3-fold increase in ALP activity between 7 and 21 days versus a minimal increase on control scaffolds. Compared to cells on PLG controls, RUNX2 expression in NHOsts on composite scaffolds was lower at both 7 and 21 days, while expression of genes encoding for bone matrix proteins (COL1A1 and SPARC) was higher on BG-PLG scaffolds at both time points. These data demonstrate the importance of selecting a ceramic when fabricating composites applied for bone healing

Searches for massive neutrinos with mechanical quantum sensors

The development of quantum optomechanics now allows mechanical sensors with femtogram masses to be controlled and measured in the quantum regime. If the mechanical element contains isotopes that undergo nuclear decay, measuring the recoil of the sensor following the decay allows reconstruction of the total momentum of all emitted particles, including any neutral particles that may escape detection in traditional detectors. As an example, for weak nuclear decays the momentum of the emitted neutrino can be reconstructed on an event-by-event basis. We present the concept that a single nanometer-scale, optically levitated sensor operated with sensitivity near the standard quantum limit can search for heavy sterile neutrinos in the keV-MeV mass range with sensitivity significantly beyond existing constraints. We also comment on the possibility that mechanical sensors operated well into the quantum regime might ultimately reach the sensitivities required to provide an absolute measurement of the mass of the light neutrino states.Comment: 11 pages + refs, 7 figures. v2: published version (+ an appendix containing a quantum model of the 3-body decay in a nanosphere

SbcCD regulation and localization in Escherichia coli

The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm

Test Excavations at the Culebra Creek Site, 41BX126, Bexar County, Texas

Archaeological test excavations were undertaken at 4IBX126 on Culebra Creek to offset the impact from a proposed Texas Department of Transportation (TxDOT) highway improvement project on Loop 1604 in northwest Bexar County. Archaeological investigations were conducted in three field seasons: the first two seasons were conducted by TxDOT archaeologists and the third was directed by personnel from the Center for Archaeological Research (CAR) of The University of Texas at San Antonio. During the three projects, 55 hand-dug units, 29 backhoe trenches, 36 shovel tests, and eight Gradall trenches were excavated. Seventeen features were recorded; 25 radiocarbon assays were conducted; over 59,000 lithic artifacts were recovered and analyzed; 1,655 liters of sediment float samples were processed; 3,337 kg of burned rock were analyzed; and nearly 300 g of fatmal material and 25 archaeomagnetic samples were analyzed. The testing revealed utilization of the site in the Early, Middle, and Late Archaic periods. The analysis of materials and results of all three field efforts are presented in this single volume. Geoarchaeological investigations show that four terraces (TO, Tl, T2, and T3) in the immediate site area accumulated from the Late Pleistocene through the Holocene. Five Stratigraphic Units (I-V) make up these terraces and overlap one another. The T2 terrace is composed of Stratigraphic Units IT, ill, and IV, while the Tl terrace consists mostly of Stratigraphic Units IV and V. Archaeological materials were discovered in situ within the Tl and T3 terraces and primarily within Stratigraphic Units ill and IV. Radiocarbon assays indicate that Stratigraphic Unit IV formed between at least 4000-2000 B.P., Stratigraphic Unit ill accumulated between approximately 11,500-4000 B.P., and Stratigraphic Unit IT was accreting at least 17,500 years ago. Too little evidence exists to determine the full time ranges of sediment accumulation, and whether significant temporal gaps exist between the sedimentation of these geological units. Archaeological excavations focused on three separate areas: A, B, and C. Area A is a new right-of-way east of the existing right-of-way. Excavations in this area defined a Late Archaic Montell component dating to approximately 2700 B.P. These materials include two burned rock features in situ within Unit IV on the scarp of the T2 terrace. This area probably was occupied by a small residential group during the Late Archaic period. Area B is east of Loop 1604 in the existing right-of-way and on the T2 terrace. Area B contains a Middle Archaic Nolan component in the upper portion of Stratigraphic Unit ill, below a Late Archaic burned rock midden with a central subsurface oven in Unit IV. Area C is in the existing right-of-way west of Loop 1604. Excavations in this area investigated the possibility of an intact Early Archaic occupation; however, no evidence of one was found. In Area B, the Nolan component consisted of lithic artifacts scattered among small burned rock features that probably served as hearths. This component is radiocarbon dated to approximately 4600 B.P. The Late Archaic burned rock midden was apparently used between 4000 B.P. and 2000 B.P. Subfeatures within the central oven indicate multiple cooking events. Ethnographic evidence suggests earth ovens contained food wrapped with insulating material over a layer of hot rocks heated by a coal bed. This was capped with dirt to seal the oven. When cooking was complete, the earth cap is removed to reach the food. CAR conducted earth-oven hot-rock experiments which indicated that local limestone could be used once or at the most twice. Local hot-rock cooking should generate a great deal of burned limestone debris. The framework ofthe feature at 41BX126 represents the cap and rock heating-element dumpings from separate cooking events as well as a few small intact burned rock features that served as ovens or hearths. At the base of the midden were a few depressions that may represent borrow pits used to obtain sediment for the central oven cap. Mixing of temporally distinct artifacts from the Nolan and later occupations occurs in and beyond the midden due to sediment excavation and transportation across the site, and redeposition of materials through erosion of materials off the framework

Commercial users panel

The discussions of motives and requirements for telerobotics application demonstrated that, in many cases, lack of progress was a result not of limited opportunities but of inadequate mechanisms and resources for promoting opportunities. Support for this conclusion came from Telerobotics, Inc., one of the few companies devoted primarily to telerobot systems. They have produced units for such diverse applications as nuclear fusion research, particle accelerators, cryogenics, firefighting, marine biology/undersea systems and nuclear mobile robotics. Mr. Flatau offered evidence that telerobotics research is only rarely supported by the private sector and that it often presents a difficult market. Questions on the mechanisms contained within the NASA technology transfer process for promoting commercial opportunities were fielded by Ray Gilbert and Tom Walters. A few points deserve emphasis: (1) NASA/industry technology transfer occurs in both directions and NASA recognizes the opportunity to learn a great deal from industry in the fields of automation and robotics; (2) promotion of technology transfer projects takes a demand side approach, with requests to industry for specific problem identification. NASA then proposes possible solutions; and (3) comittment ofmotivated and technically qualified people on each end of a technology transfer is essential

Sequence-Specific, Nanomolar Peptide Binding via Cucurbit[8]uril-Induced Folding and Inclusion of Neighboring Side Chains

This paper describes the molecular recognition of the tripeptide Tyr-Leu-Ala by the synthetic receptor cucurbit[8]uril (Q8) in aqueous buffer with nanomolar affinity and exceptional specificity. This combination of characteristics, which also applies to antibodies, is desirable for applications in biochemistry and biotechnology but has eluded supramolecular chemists for decades. Building on prior knowledge that Q8 binds to peptides with N-terminal aromatic residues, a library screen of 105 peptides was designed to test the effects of residues adjacent to N-terminal Trp, Phe, or Tyr. The screen used tetramethylbenzobis(imidazolium) (MBBI) as a fluorescent indicator and resulted in the unexpected discovery that MBBI can serve not only as a turn-off sensor via the simultaneous inclusion of a Trp residue but also as a turn-on sensor via the competitive displacement of MBBI upon binding of Phe- or Tyr-terminated peptides. The unusual fluorescence response of the Tyr series prompted further investigation by 1H NMR spectroscopy, electrospray ionization mass spectrometry, and isothermal titration calorimetry. From these studies, a novel binding motif was discovered in which only 1 equiv of peptide binds to Q8, and the side chains of both the N-terminal Tyr residue and its immediate neighbor bind within the Q8 cavity. For the peptide Tyr-Leu-Ala, the equilibrium dissociation constant value is 7.2 nM, whereas that of its sequence isomer Tyr-Ala-Leu is 34 μM. The high stability, recyclability, and low cost of Q8 combined with the straightforward incorporation of Tyr-Leu-Ala into recombinant proteins should make this system attractive for the development of biological applications

CRANKITE: a fast polypeptide backbone conformation sampler

Background: CRANKITE is a suite of programs for simulating backbone conformations of polypeptides and proteins. The core of the suite is an efficient Metropolis Monte Carlo sampler of backbone conformations in continuous three-dimensional space in atomic details. Methods: In contrast to other programs relying on local Metropolis moves in the space of dihedral angles, our sampler utilizes local crankshaft rotations of rigid peptide bonds in Cartesian space. Results: The sampler allows fast simulation and analysis of secondary structure formation and conformational changes for proteins of average length

Ample evidence for fish sentience and pain

The majority of commentaries are supportive of our position on the scepticism that muddies the waters surrounding fish pain and sentience. There is substantial empirical evidence for pain in fish. Animals’ experience of pain cannot be compared to artificial intelligence (AI) because AI can only mimic responses to nociceptive input on the basis of human observations and programming. Accepting that fish are sentient would not be detrimental to the industries reliant on fish. A more proactive discussion between scientists and stakeholders is needed to improve fish welfare for the benefit of all