7 research outputs found
Impact of ceftiofur Injection on gut microbiota and <em>Escherichia coli</em> resistance in pigs
International audienceResistance to extended-spectrum cephalosporins (ESCs) is an important health concern. Here, we studied the impact of the administration of a long-acting form of ceftiofur on the pig gut microbiota and ESC resistance in Escherichia coli. Pigs were orally inoculated with an ESC-resistant E. coli M63 strain harboring a conjugative plasmid carrying a gene conferring resistance, blaCTX-M-1. On the same day, they were given or not a unique injection of ceftiofur. Fecal microbiota were studied using quantitative PCR analysis of the main bacterial groups and quantification of short-chain fatty acids. E. coli and ESC-resistant E. coli were determined by culture methods, and the ESC-resistant E. coli isolates were characterized. The copies of the blaCTX-M-1 gene were quantified. After ceftiofur injection, the main change in gut microbiota was the significant but transitory decrease in the E. coli population. Acetate and butyrate levels were significantly lower in the treated group. In all inoculated groups, E. coli M63 persisted in most pigs, and the blaCTX-M-1 gene was transferred to other E. coli. Culture and PCR results showed that the ceftiofurtreated group shed significantly more resistant strains 1 and 3 days after ESC injection. Thereafter, on most dates, there were no differences between the groups, but notably, one pig in the nontreated group regularly excreted very high numbers of ESC-resistant E. coli, probably leading to a higher contamination level in its pen. In conclusion, the use of ESCs, and also the presence of high-shedding animals, are important features in the spread of ESC resistance
Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods
This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28 degrees C for 24-28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (<= 1 mu g ml-1), gentamicin (<= 4 mu g ml-1), oxytetracycline (<= 0.25 mu g ml-1) and trimethoprim/sulfamethoxazole (<= 0.125/2.38 mu g ml-1). The cut-off values for disc zone data were calculated for enrofloxacin (>= 29 mm), florfenicol (>= 27 mm), gentamicin (>= 19 mm), oxolinic acid (>= 24 mm), oxytetracycline (>= 24 mm) and trimethoprim/sulfamethoxazole (>= 26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values
Validation of an antimicrobial susceptibility testing protocol for Brachyspira hyodysenteriae and Brachyspira pilosicoli in an international ring trial
Brachyspira hyodysenteriae and Brachyspira pilosicoli cause economically important enteric disease in pigs. Treatment of these infections often includes antimicrobial administration, which can be most effective when therapeutic options are informed by antimicrobial susceptibility testing data. Here we describe a method for broth dilution antimicrobial susceptibility testing of these bacteria, both of which are difficult to culture in vitro. The protocol was evaluated for its fitness for use in an inter-laboratory ring trial involving eight laboratories from seven countries, and employing eleven test strains (5 Brachyspira hyodysenteriae including the type strain B78T and 6 Brachyspira pilosicoli) and six antibiotics. Overall intra- and inter-laboratory reproducibility of this method was very good (>90 % MICs at mode +/- 1 log2). Whole genome sequencing revealed good correspondence between reduced susceptibility and the presence of previously defined antimicrobial resistance determinants. Interestingly, lnu(C) was identified in B. pilosicoli isolates with elevated MICs of lincomycin, whilst tva(B) was associated with elevated MICs of pleuromutilins in this species. We designated two new control strains with MICs lying within currently tested ranges, including for the pleuromutilins, in contrast to the control strain B. hyodysenteriae B78T. These were deposited at the DSMZ-German Collection of Microorganisms and Cell Cultures GmbH. The validation of a standard protocol and identification of new control strains facilitates comparisons between studies, establishment of robust interpretative criteria, and ultimately contributes to rational antimicrobial use when treating infected livestock.</p
Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods
This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24-28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (â€1 ”g ml-1), gentamicin (â€4 ”g ml-1), oxytetracycline (â€0.25 ”g ml-1) and trimethoprim/sulfamethoxazole (â€0.125/2.38 ”g ml-1). The cut-off values for disc zone data were calculated for enrofloxacin (â„29 mm), florfenicol (â„27 mm), gentamicin (â„19 mm), oxolinic acid (â„24 mm), oxytetracycline (â„24 mm) and trimethoprim/sulfamethoxazole (â„26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.European CommissionMinistĂšre de l'Agriculture et de la SouverainetĂ© alimentaire (France)MinistĂšre de la Transition Ă©cologique et de la CohĂ©sion des territoires (France)Norwegian Environmental AgencyMinistero della Salute (Italia)Depto. de Sanidad AnimalCentro de Vigilancia Sanitaria Veterinaria (VISAVET)Fac. de VeterinariaTRUEpu
Comparison of Extended-Spectrum ÎČ-Lactamase (ESBL) CTX-M Genotypes in Franklin Gulls from Canada and Chile
Antibiotic resistance patterns in Escherichia coli from gulls in nine European countries
Background: The prevalence of antibiotic resistant faecal indicator bacteria from humans and food production
animals has increased over the last decades. In Europe, resistance levels in Escherichia coli from these sources
show a south-to-north gradient, with more widespread resistance in the Mediterranean region compared
to northern Europe. Recent studies show that resistance levels can be high also in wildlife, but it is unknown to
what extent resistance levels in nature conform to the patterns observed in human-associated bacteria.
Methods: To test this, we collected 3,158 faecal samples from breeding gulls (Larus sp.) from nine European
countries and tested 2,210 randomly isolated E. coli for resistance against 10 antibiotics commonly used in
human and veterinary medicine.
Results: Overall, 31.5% of the gull E. coli isolates were resistant to ]1 antibiotic, but with considerable
variation between countries: highest levels of isolates resistant to ]1 antibiotic were observed in Spain
(61.2%) and lowest levels in Denmark (8.3%). For each tested antibiotic, the Iberian countries were either the
countries with the highest levels or in the upper range in between-country comparisons, while northern
countries generally had a lower proportion of resistant E. coli isolates, thereby resembling the gradient of
resistance seen in human and food animal sources.
Conclusion: We propose that gulls may serve as a sentinel of environmental levels of antibiotic resistant
E. coli to complement studies of human-associated microbiota.Swedish
Research Council FORMASThe Karin
Korsnerâs FoundationThe Olle Engkvist Byggmašstare
FoundationAD 22/01/201