Trasplante de células madre hematopoyéticas a embriones preinmunes vía celocentesis

La terapia celular mediante la introducción de células madre hematopoyéticas en un paciente afecto de una enfermedad susceptible de tratamiento, es una forma de terapia génica. El alo-trasplante de células madre hematopoyéticas ocurre de forma natural en embarazos gemelares dicigóticos, que establecen comunicaciones placentarias en útero. Esto se debe a que diversas características del embrión en desarrollo, brindan la oportunidad de tolerar sangre de un donante durante este periodo. Si se perfeccionan los métodos de introducción de las células madre en el embrión, este tipo de tratamiento genético en útero, podría convertirse en una alternativa viable, sobre todo para tratar enfermedades metabólicas, inmunológicas y hematopoyéticas, de origen hereditario, antes de que el déficit genético haya comprometido significativamente a distintos órganos fetales. Además, la existencia de una "ventana inmunológica" en el desarrollo embrionario, previa al inicio de los procesos de capacitación tímica, con presencia de células T maduras, permite la tolerancia a antígenos extraños. En condiciones ideales, la transferencia génica iniciada durante este periodo daría lugar a una tolerancia prolongada, quizás permanente, si estos antígenos se mantienen de forma persistente, lo que evitaría cualquier respuesta inmune celular del huésped contra las células donadas y facilitaría el trasplante de otros órganos en un período postnatal. Nuestra hipótesis se basa en demostrar, mediante el trasplante de células madre hematopoyéticas humanas CD34+ a embriones babuinos, entre los días 36 y 46 postfecundación vía celocentesis, ecoguiada, la posibilidad de crear xenoquimeras hematopoyéticas humano-babuino. Con este fin, se aislaron por métodos inmunomagnéticos, células madre CD34+ procedentes de las sangre del cordón umbilical de recién nacidos a término y sin complicaciones intraparto..

Trasplante de células madre hematopoyéticas a embriones preinmunes vía celocentesis

Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Obstetricia y Ginecología, leída el 29-06-2004La terapia celular mediante la introducción de células madre hematopoyéticas en un paciente afecto de una enfermedad susceptible de tratamiento, es una forma de terapia génica. El alo-trasplante de células madre hematopoyéticas ocurre de forma natural en embarazos gemelares dicigóticos, que establecen comunicaciones placentarias en útero. Esto se debe a que diversas características del embrión en desarrollo, brindan la oportunidad de tolerar sangre de un donante durante este periodo. Si se perfeccionan los métodos de introducción de las células madre en el embrión, este tipo de tratamiento genético en útero, podría convertirse en una alternativa viable, sobre todo para tratar enfermedades metabólicas, inmunológicas y hematopoyéticas, de origen hereditario, antes de que el déficit genético haya comprometido significativamente a distintos órganos fetales. Además, la existencia de una "ventana inmunológica" en el desarrollo embrionario, previa al inicio de los procesos de capacitación tímica, con presencia de células T maduras, permite la tolerancia a antígenos extraños. En condiciones ideales, la transferencia génica iniciada durante este periodo daría lugar a una tolerancia prolongada, quizás permanente, si estos antígenos se mantienen de forma persistente, lo que evitaría cualquier respuesta inmune celular del huésped contra las células donadas y facilitaría el trasplante de otros órganos en un período postnatal. Nuestra hipótesis se basa en demostrar, mediante el trasplante de células madre hematopoyéticas humanas CD34+ a embriones babuinos, entre los días 36 y 46 postfecundación vía celocentesis, ecoguiada, la posibilidad de crear xenoquimeras hematopoyéticas humano-babuino. Con este fin, se aislaron por métodos inmunomagnéticos, células madre CD34+ procedentes de las sangre del cordón umbilical de recién nacidos a término y sin complicaciones intraparto...Depto. de Salud Pública y Materno - InfantilFac. de MedicinaTRUEunpu

Random Generation of Arbitrary Waveforms for Emulating Three-Phase Systems

This paper describes an apparatus for generating a signal representative of steady-state and transient disturbances in three-phase waveforms of an ac electrical system as described in IEEE Std 1159-09. It can be configured as a synthesizer of randomly distorted signals for different applications: for testing the effects of disturbed grid on equipment and to generate patterns of electrical disturbances for the training of artificial neural networks, which are used for measuring power quality tasks. For the first purpose, voltage and current amplifiers are added in the output stage, which allows the generation of disturbed signals at grid level.Comisión Interministerial de Ciencia y Tecnología DPI2006-15467-C02-01Comisión Interministerial de Ciencia y Tecnología DPI2006-15467-C02-0

Electricity clustering framework for automatic classification of customer loads

Clustering in energy markets is a top topic with high significance on expert and intelligent systems. The main impact of is paper is the proposal of a new clustering framework for the automatic classification of electricity customers’ loads. An automatic selection of the clustering classification algorithm is also highlighted. Finally, new customers can be assigned to a predefined set of clusters in the classificationphase. The computation time of the proposed framework is less than that of previous classification tech- niques, which enables the processing of a complete electric company sample in a matter of minutes on a personal computer. The high accuracy of the predicted classification results verifies the performance of the clustering technique. This classification phase is of significant assistance in interpreting the results, and the simplicity of the clustering phase is sufficient to demonstrate the quality of the complete mining framework.Ministerio de Economía y Competitividad TEC2013-40767-RMinisterio de Economía y Competitividad IDI- 2015004

Search for flavour-changing neutral currents in processes with one top quark and a photon using 81 fb−1 of pp collisions at √s = 13 TeV with the ATLAS experiment

Artículo escrito por un elevado número de autores, sólo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiera, y los autores pertenecientes a la UAMA search for flavour-changing neutral current (FCNC) events via the coupling of a top quark, a photon, and an up or charm quark is presented using 81 fb−1 of proton–proton collision data taken at a centre-of-mass energy of 13 TeV with the ATLAS detector at the LHC. Events with a photon, an electron or muon, a b-tagged jet, and missing transverse momentum are selected. A neural network based on kinematic variables differentiates between events from signal and background processes. The data are consistent with the background-only hypothesis, and limits are set on the strength of the tqγ coupling in an effective field theory. These are also interpreted as 95% CL upper limits on the cross section for FCNC tγ production via a left-handed (right-handed) tuγ coupling of 36 fb (78 fb) and on the branching ratio for t→γu of 2.8×10−5 (6.1×10−5). In addition, they are interpreted as 95% CL upper limits on the cross section for FCNC tγ production via a left-handed (right-handed) tcγ coupling of 40 fb (33 fb) and on the branching ratio for t→γc of 22×10−5 (18×10−5)We acknowledge the support of ANPCyT, Argentina; YerPhI, Armenia; ARC, Australia; BMWFW and FWF, Austria; ANAS, Azerbaijan; SSTC, Belarus; CNPq and FAPESP, Brazil; NSERC, NRC and CFI, Canada; CERN; CONICYT, Chile; CAS, MOST and NSFC, China; COLCIENCIAS, Colombia; MSMT CR, MPO CR and VSC CR, Czech Republic; DNRF and DNSRC, Denmark; IN2P3-CNRS, CEA-DRF/IRFU, France; SRNSFG, Georgia; BMBF, HGF, and MPG, Germany; GSRT, Greece; RGC, Hong Kong SAR, China; ISF and Benoziyo Center, Israel; INFN, Italy; MEXT and JSPS, Japan; CNRST, Morocco; NWO, Netherlands; RCN, Norway; MNiSW and NCN, Poland; FCT, Portugal; MNE/IFA, Romania; MES of Russia and NRC KI, Russian Federation; JINR; MESTD, Serbia; MSSR, Slovakia; ARRS and MIZŠ, Slovenia; DST/NRF, South Africa; MINECO, Spain; SRC and Wallenberg Foundation, Sweden; SERI, SNSF and Cantons of Bern and Geneva, Switzerland; MOST, Taiwan; TAEK, Turkey; STFC, United Kingdom; DOE and NSF, United States of America. In addition, individual groups and members have received support from BCKDF, Canarie, CRC and Compute Canada, Canada; COST, ERC, ERDF, Horizon 2020, and Marie Skłodowska-Curie Actions, European Union; Investissements d' Avenir Labex and Idex, ANR, France; DFG and AvH Foundation, Germany; Herakleitos, Thales and Aristeia programmes co-financed by EU-ESF and the Greek NSRF, Greece; BSF-NSF and GIF, Israel; CERCA Programme Generalitat de Catalunya, Spain; The Royal Society and Leverhulme Trust, United Kingdom. The crucial computing support from all WLCG partners is acknowledged gratefully, in particular from CERN, the ATLAS Tier-1 facilities at TRIUMF (Canada), NDGF (Denmark, Norway, Sweden), CC-IN2P3 (France), KIT/GridKA (Germany), INFN-CNAF (Italy), NL-T1 (Netherlands), PIC (Spain), ASGC (Taiwan), RAL (UK) and BNL (USA), the Tier-2 facilities worldwide and large non-WLCG resource provider

Aproximación paralela a la técnica Divide y Vencerás

En este trabajo se presenta una propuesta de desarrollo del tema dedicado a la técnica Divide y Vencerás para una asignatura de Programación en Paralelo. Tras una breve introducción a la técnica desde el punto de vista secuencial, se abordan, mediante un ejemplo, las distintas posibilidades de paralelización. El nivel de dificultad se incrementa de forma gradual desde una implementación para Memoria Compartida hasta una para Paso de Mensajes. Finalmente se estudia el rendimiento de las propuestas paralelas frente a las secuenciales

Soil temperature determines the reaction of olive cultivars to verticillium dahliae pathotypes

Development of Verticillium wilt in olive, caused by the soil-borne fungus Verticillium dahliae, can be influenced by biotic and environmental factors. In this study we modeled i) the combined effects of biotic factors (i.e., pathotype virulence and cultivar susceptibility) and abiotic factors (i.e., soil temperature) on disease development and ii) the relationship between disease severity and several remote sensing parameters and plant stress indicators. Methodology: Plants of Arbequina and Picual olive cultivars inoculated with isolates of defoliating and non-defoliating V. dahliae pathotypes were grown in soil tanks with a range of soil temperatures from 16 to 32°C. Disease progression was correlated with plant stress parameters (i.e., leaf temperature, steady-state chlorophyll fluorescence, photochemical reflectance index, chlorophyll content, and ethylene production) and plant growth-related parameters (i.e., canopy length and dry weight). Findings: Disease development in plants infected with the defoliating pathotype was faster and more severe in Picual. Models estimated that infection with the defoliating pathotype was promoted by soil temperatures in a range of 16 to 24°C in cv. Picual and of 20 to 24°C in cv. Arbequina. In the non-defoliating pathotype, soil temperatures ranging from 16 to 20°C were estimated to be most favorable for infection. The relationship between stress-related parameters and disease severity determined by multinomial logistic regression and classification trees was able to detect the effects of V. dahliae infection and colonization on water flow that eventually cause water stress. Conclusions: Chlorophyll content, steady-state chlorophyll fluorescence, and leaf temperature were the best indicators for Verticillium wilt detection at early stages of disease development, while ethylene production and photochemical reflectance index were indicators for disease detection at advanced stages. These results provide a better understanding of the differential geographic distribution of V. dahliae pathotypes and to assess the potential effect of climate change on Verticillium wilt development.Financial support for this research was provided by Project P08-AGR-03528 from ‘‘Consejería de Economía, Innovación y Ciencia’’ of Junta de Andalucía and the European Social Fund (JANC), and projects AGL-2012-37521 (JANC) and AGL2012-40053-C03-01 (PJZT) from the Spanish ‘‘Ministerio de Economia y Competitividad’’ and the European Social Fund. R. Calderón is a recipient of research fellowship BES-2010-035511 from the Spanish ‘‘Ministerio de Ciencia e Innovación’’ and C. Lucena was a recipient of a JAE-DOC postdoctoral contract from ‘‘Consejo Superior de Investigaciones Científicas’’ (CSIC) co-funded by the European Social Fund. TPeer Reviewe

Protocolo de estudio cuasi-experimental para promover un estilo interpersonal de apoyo a la autonomía en docentes de educación física

Según la Teoría de la Autodeterminación (Deci y Ryan, 1985, 2002), el estilo interpersonal que utilice el educador puede tener un fuerte impacto en la motivación de los estudiantes. Los objetivos de este artículo fueron: (i) explicar la justificación y desarrollo de un programa de formación basado en esta teoría para promover el apoyo a la autonomía; (ii) describir el protocolo de estudio y los procedimientos de evaluación del programa de formación; y (iii) probar la integridad del protocolo de estudio para una futura intervención. De un total de 40 docentes de educación física, se asignarán 20 a un grupo de intervención o a un grupo control (n = 20). Durante un período de 8 semanas, los docentes del grupo de intervención completarán un programa de formación en apoyo a la autonomía a través de varias fases. Se evaluarán indicadores que caracterizan el estilo interpersonal de apoyo a la autonomía de forma cualitativa y cuantitativa, tanto al docente como al estudiante. La recogida de los datos de la línea base cuantitativa se llevará a cabo un mes antes del inicio de la intervención y un mes después de la finalización del período de intervención. Los datos cuantitativos se analizarán mediante análisis multinivel. Para explorar las percepciones del programa de formación, también se llevará a cabo un seguimiento a través de entrevistas semiestructuradas con docentes y miembros de la clase, de cuatro a seis semanas después del final de la intervención. Este estudio cuenta con la aprobación del Comité de Ética de las instituciones participantes. Los hallazgos del estudio se difundirán a través de revistas de impacto científico y presentaciones en conferencias sobre la temática de estudio de carácter internacional

Search for displaced vertices of oppositely charged leptons from decays of long-lived particles in pp collisions at √s = 13 TeV with the ATLAS detector

Artículo escrito por un elevado número de autores, sólo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiera, y los autores pertenecientes a la UAMThe Author(s) A search for long-lived particles decaying into an oppositely charged lepton pair, μμ, ee, or eμ, is presented using 32.8fb−1 of pp collision data collected at √s=13 TeV by the ATLAS detector at the LHC. Candidate leptons are required to form a vertex, within the inner tracking volume of ATLAS, displaced from the primary pp interaction region. No lepton pairs with an invariant mass greater than 12 GeV are observed, consistent with the background expectations derived from data. The detection efficiencies for generic resonances with lifetimes (cτ) of 100–1000 mm decaying into a dilepton pair with masses between 0.1–1.0 TeV are presented as a function of pT and decay radius of the resonances to allow the extraction of upper limits on the cross sections for theoretical models. The result is also interpreted in a supersymmetric model in which the lightest neutralino, produced via squark–antisquark production, decays into ℓ+ℓ′−ν (ℓ,ℓ′=e, μ) with a finite lifetime due to the presence of R-parity violating couplings. Cross-section limits are presented for specific squark and neutralino masses. For a 700 GeV squark, neutralinos with masses of 50–500 GeV and mean proper lifetimes corresponding to cτ values between 1 mm to 6 m are excluded. For a 1.6 TeV squark, cτ values between 3 mm to 1 m are excluded for 1.3 TeV neutralinosWe acknowledge the support of ANPCyT, Argentina; YerPhI, Armenia; ARC, Australia; BMWFW and FWF, Austria; ANAS, Azerbaijan; SSTC, Belarus; CNPq and FAPESP, Brazil; NSERC, NRC and CFI, Canada; CERN; CONICYT, Chile; CAS, MOST and NSFC, China; COLCIENCIAS, Colombia; MSMT CR, MPO CR and VSC CR, Czech Republic; DNRF and DNSRC, Denmark; IN2P3-CNRS, CEA-DRF/IRFU, France; SRNSFG, Georgia; BMBF, HGF, and MPG, Germany; GSRT, Greece; RGC, Hong Kong SAR, China; ISF and Benoziyo Center, Israel; INFN, Italy; MEXT and JSPS, Japan; CNRST, Morocco; NWO, Netherlands; RCN, Norway; MNiSW and NCN, Poland; FCT, Portugal; MNE/IFA, Romania; MES of Russia and NRC KI, Russian Federation; JINR; MESTD, Serbia; MSSR, Slovakia; ARRS and MIZŠ, Slovenia; DST/NRF, South Africa; MINECO, Spain; SRC and Wallenberg Foundation, Sweden; SERI, SNSF and Cantons of Bern and Geneva, Switzerland; MOST, Taiwan; TAEK, Turkey; STFC, United Kingdom; DOE and NSF, United States of America. In addition, individual groups and members have received support from BCKDF, Canarie, CRC and Compute Canada, Canada; COST, ERC, ERDF, Horizon 2020, and Marie Skłodowska-Curie Actions, European Union; Investissements d'Avenir Labex and Idex, ANR, France; DFG and AvH Foundation, Germany; Herakleitos, Thales and Aristeia programmes co-financed by EU-ESF and the Greek NSRF, Greece; BSF-NSF and GIF, Israel; CERCA Programme Generalitat de Catalunya, Spain; The Royal Society and Leverhulme Trust, United Kingdom. The crucial computing support from all WLCG partners is acknowledged gratefully, in particular from CERN, the ATLAS Tier-1 facilities at TRIUMF (Canada), NDGF (Denmark, Norway, Sweden), CC-IN2P3 (France), KIT/GridKA (Germany), INFN-CNAF (Italy), NL-T1 (Netherlands), PIC (Spain), ASGC (Taiwan), RAL (UK) and BNL (USA), the Tier-2 facilities worldwide and large non-WLCG resource provider

Conditioning factors of linearized wood's function lactation curve shape parameters, milk yield, fat and protein content in murciano-granadina primiparous does

Research Areas: Agriculture ; Veterinary Sciences ; ZoologyA total of 137,927 controls of 22,932 Murciano-Granadina first lactation goats (measured between 1996–2016) were evaluated to determine the influence of the number of kids, season, year and farm on total milk yield, daily milk yield, lactation length, total production of fat and protein and percentages of fat and protein. All factors analyzed had a significant effect on the variables studied, except for the influence of the number of kids on the percentages of fat and protein, where the variation was very small. Goats with two offspring produced nearly 15% more milk, fat and protein per lactation compared to goats with simple kids. Kiddings occurring in summer–autumn resulted in average milk, fat and protein yields nearly 14, 19 and 23% higher when compared to winter–spring kiddings. Lactation curves were evaluated to determine the effects of the number of kids and season, using the linearized version of the model of Wood in random regression analyses. Peak Yield increased by about 0.3 kg per additional offspring at kidding, but persistence was higher in goats with single offspring. The kidding season significantly influenced the lactation curve shape. Hence summer-kidding goats were more productive, and peak occurred earlier, while a higher persistence was observed in goats kidding during autumn.info:eu-repo/semantics/publishedVersio