Infrared point source variability between the Spitzer and MSX surveys of the Galactic mid-plane

We present a list of 552 sources with suspected variability, based on a comparison of mid-infrared photometry from the GLIMPSE I and MSX surveys, which were carried out nearly a decade apart. We were careful to address issues such as the difference in resolution and sensitivity between the two surveys, as well as the differences in the spectral responses of the instruments. We selected only sources where the IRAC 8.0 and MSX 8.28 micron fluxes differ by more than a factor of two, in order to minimize contamination from sources where the difference in fluxes at 8 micron is due to a strong 10 micron silicate feature. We present a subset of 40 sources for which additional evidence suggests variability, using 2MASS and MIPSGAL data. Based on a comparison with the variability flags in the IRAS and MSX Point-Source Catalogs we estimate that at least a quarter of the 552 sources, and at least half of the 40 sources are truly variable. In addition, we tentatively confirm the variability of one source using multi-epoch IRAS LRS spectra. We suggest that most of the sources in our list are likely to be Asymptotic Giant Branch stars.Comment: 47 pages, 12 Figures, 3 Tables, accepted for publication in A

Inhibition of mTORC1 inhibits lytic replication of Epstein-Barr virus in a cell-type specific manner

BACKGROUND: Epstein-Barr virus is a human herpesvirus that infects a majority of the human population. Primary infection of Epstein-Barr virus (EBV) causes the syndrome infectious mononucleosis. This virus is also associated with several cancers, including Burkitt’s lymphoma, post-transplant lymphoproliferative disorder and nasopharyngeal carcinoma. As all herpesvirus family members, EBV initially replicates lytically to produce abundant virus particles, then enters a latent state to remain within the host indefinitely. METHODS: Through a genetic screen in Drosophila, we determined that reduction of Drosophila Tor activity altered EBV immediate-early protein function. To further investigate this finding, we inhibited mTOR in EBV-positive cells and investigated subsequent changes to lytic replication via Western blotting, flow cytometry, and quantitative PCR. The student T-test was used to evaluate significance. RESULTS: mTOR, the human homolog of Drosophila Tor, is an important protein at the center of a major signaling pathway that controls many aspects of cell biology. As the EBV immediate-early genes are responsible for EBV lytic replication, we examined the effect of inhibition of mTORC1 on EBV lytic replication in human EBV-positive cell lines. We determined that treatment of cells with rapamycin, which is an inhibitor of mTORC1 activity, led to a reduction in the ability of B cell lines to undergo lytic replication. In contrast, EBV-positive epithelial cell lines underwent higher levels of lytic replication when treated with rapamycin. CONCLUSIONS: Overall, the responses of EBV-positive cell lines vary when treated with mTOR inhibitors, and this may be important when considering such inhibitors as anti-cancer therapeutic agents

Orthogonal-view Microscope for the Biomechanics Investigations of Aquatic Organisms

Microscopes are essential for biomechanics and hydrodynamical investigation of small aquatic organisms. We report a DIY microscope (GLUBscope) that enables the visualization of organisms from two orthogonal imaging planes (top and side views). Compared to conventional imaging systems, this approach provides a comprehensive visualization strategy of organisms, which could have complex shapes and morphologies. The microscope was constructed by combining custom 3D-printed parts and off-the-shelf components. The system is designed for modularity and reconfigurability. Open-source design files and build instructions are provided in this report. Additionally, proof of use experiments, particularly with Hydra and other organisms that combine the GLUBscope with an analysis pipeline, were demonstrated. Beyond the applications demonstrated, the system can be used or modified for various imaging applications

Spitzer infrared spectrometer 16μm observations of the GOODS fields

We present Spitzer 16μm imaging of the Great Observatories Origins Deep Survey (GOODS) fields. We survey 150 arcmin^2 in each of the two GOODS fields (North and South), to an average 3σ depth of 40 and 65 μJy, respectively. We detect ~1300 sources in both fields combined. We validate the photometry using the 3–24μm spectral energy distribution of stars in the fields compared to Spitzer spectroscopic templates. Comparison with ISOCAM and AKARI observations in the same fields shows reasonable agreement, though the uncertainties are large. We provide a catalog of photometry, with sources cross-correlated with available Spitzer, Chandra, and Hubble Space Telescope data. Galaxy number counts show good agreement with previous results from ISOCAM and AKARI with improved uncertainties. We examine the 16–24μm flux ratio and find that for most sources it lies within the expected locus for starbursts and infrared luminous galaxies. A color cut of S_(16)/S_(24) > 1.4 selects mostly sources which lie at 1.1 < z < 1.6, where the 24μm passband contains both the redshifted 9.7 μm silicate absorption and the minimum between polycyclic aromatic hydrocarbon emission peaks. We measure the integrated galaxy light of 16μm sources and find a lower limit on the galaxy contribution to the extragalactic background light at this wavelength to be 2.2 ± 0.2 nW m^(−2) sr^(−1)

Topological entropy of a stiff ring polymer and its connection to DNA knots

We discuss the entropy of a circular polymer under a topological constraint. We call it the {\it topological entropy} of the polymer, in short. A ring polymer does not change its topology (knot type) under any thermal fluctuations. Through numerical simulations using some knot invariants, we show that the topological entropy of a stiff ring polymer with a fixed knot is described by a scaling formula as a function of the thickness and length of the circular chain. The result is consistent with the viewpoint that for stiff polymers such as DNAs, the length and diameter of the chains should play a central role in their statistical and dynamical properties. Furthermore, we show that the new formula extends a known theoretical formula for DNA knots.Comment: 14pages,11figure

Microfluidics for Hydrodynamics Investigations of Sand Dollar Larvae

The life cycle of most marine invertebrates includes a planktonic larval stage before metamorphosis to bottom-dwelling adulthood. During larval stage, ciliary-mediated activity enables feeding (capture unicellular algae) and transport of materials (oxygen) required for the larva's growth, development, and successful metamorphosis. Investigating the underlying hydrodynamics of these behaviors is valuable for addressing fundamental biological questions (e.g., phenotypic plasticity) and advancing engineering applications. In this work, we combined microfluidics and fluorescence microscopy as a miniaturized PIV (mPIV) to study ciliary-medicated hydrodynamics during suspension feeding in sand dollar larvae (Dendraster excentricus). First, we confirmed the approach's feasibility by examining the underlying hydrodynamics (vortex patterns) for low- and high-fed larvae. Next, ciliary hydrodynamics were tracked from 11 days post-fertilization (DPF) to 20 DPF for 21 low-fed larvae. Microfluidics enabled the examination of baseline activities (without external flow) and behaviors in the presence of environmental cues (external flow). A library of qualitative vortex patterns and quantitative hydrodynamics was generated and shared as a stand alone repository. Results from mPIV (velocities) were used to examine the role of ciliary activity in transporting materials (oxygen). Given the laminar flow and the viscosity-dominated environments surrounding the larvae, overcoming the diffusive boundary layer is critical for the organism's survival. Peclet number analysis for oxygen transport suggested that ciliary velocities help overcome the diffusion dominated transport (max Pe numbers between 30-60). Microfluidics serving as mPIV provided a scalable and accessible approach for investigating the ciliary hydrodynamics of marine organisms.Comment: 21 pages and 11 figures (videos not included

The Conformal Sector of F-theory GUTs

D3-brane probes of exceptional Yukawa points in F-theory GUTs are natural hidden sectors for particle phenomenology. We find that coupling the probe to the MSSM yields a new class of N = 1 conformal fixed points with computable infrared R-charges. Quite surprisingly, we find that the MSSM only weakly mixes with the strongly coupled sector in the sense that the MSSM fields pick up small exactly computable anomalous dimensions. Additionally, we find that although the states of the probe sector transform as complete GUT multiplets, their coupling to Standard Model fields leads to a calculable threshold correction to the running of the visible sector gauge couplings which improves precision unification. We also briefly consider scenarios in which SUSY is broken in the hidden sector. This leads to a gauge mediated spectrum for the gauginos and first two superpartner generations, with additional contributions to the third generation superpartners and Higgs sector.Comment: v2: 51 pages, 2 figures, remark added, typos correcte

Near-Earth plasma sheet boundary dynamics during substorm dipolarization.

We report on the large-scale evolution of dipolarization in the near-Earth plasma sheet during an intense (AL ~ -1000 nT) substorm on August 10, 2016, when multiple spacecraft at radial distances between 4 and 15 R E were present in the night-side magnetosphere. This global dipolarization consisted of multiple short-timescale (a couple of minutes) B z disturbances detected by spacecraft distributed over 9 MLT, consistent with the large-scale substorm current wedge observed by ground-based magnetometers. The four spacecraft of the Magnetospheric Multiscale were located in the southern hemisphere plasma sheet and observed fast flow disturbances associated with this dipolarization. The high-time-resolution measurements from MMS enable us to detect the rapid motion of the field structures and flow disturbances separately. A distinct pattern of the flow and field disturbance near the plasma boundaries was found. We suggest that a vortex motion created around the localized flows resulted in another field-aligned current system at the off-equatorial side of the BBF-associated R1/R2 systems, as was predicted by the MHD simulation of a localized reconnection jet. The observations by GOES and Geotail, which were located in the opposite hemisphere and local time, support this view. We demonstrate that the processes of both Earthward flow braking and of accumulated magnetic flux evolving tailward also control the dynamics in the boundary region of the near-Earth plasma sheet.Graphical AbstractMultispacecraft observations of dipolarization (left panel). Magnetic field component normal to the current sheet (BZ) observed in the night side magnetosphere are plotted from post-midnight to premidnight region: a GOES 13, b Van Allen Probe-A, c GOES 14, d GOES 15, e MMS3, g Geotail, h Cluster 1, together with f a combined product of energy spectra of electrons from MMS1 and MMS3 and i auroral electrojet indices. Spacecraft location in the GSM X-Y plane (upper right panel). Colorcoded By disturbances around the reconnection jets from the MHD simulation of the reconnection by Birn and Hesse (1996) (lower right panel). MMS and GOES 14-15 observed disturbances similar to those at the location indicated by arrows

Reaction of O2 with a di-iron protein generates a mixed valent Fe2+/Fe3+ center and peroxide

The gene encoding the cyanobacterial ferritin SynFtn is up-regulated in response to copper stress. Here, we show that, while SynFtn does not interact directly with copper, it is highly unusual in several ways. First, its catalytic diiron ferroxidase center is unlike those of all other characterized prokaryotic ferritins and instead resembles an animal H-chain ferritin center. Second, as demonstrated by kinetic, spectroscopic, and high-resolution X-ray crystallographic data, reaction of O2 with the di-Fe2+ center results in a direct, one-electron oxidation to a mixed-valent Fe2+/Fe3+ form. Iron–O2 chemistry of this type is currently unknown among the growing family of proteins that bind a diiron site within a four α-helical bundle in general and ferritins in particular. The mixed-valent form, which slowly oxidized to the more usual di-Fe3+ form, is an intermediate that is continually generated during mineralization. Peroxide, rather than superoxide, is shown to be the product of O2 reduction, implying that ferroxidase centers function in pairs via long-range electron transfer through the protein resulting in reduction of O2 bound at only one of the centers. We show that electron transfer is mediated by the transient formation of a radical on Tyr40, which lies ∼4 Å from the diiron center. As well as demonstrating an expansion of the iron–O2 chemistry known to occur in nature, these data are also highly relevant to the question of whether all ferritins mineralize iron via a common mechanism, providing unequivocal proof that they do not