Intra and interspecific variation assessment in Psocoptera using near spectoscopy

Several species of Psocoptera are associated with and damage grains and other stored products, books, historical documents, and insect collections. Their small size and lack of expressive morphological variation make it a difficult group for species identification. The spectra of adult males and females of 10 psocid species from the genus Liposcelis were obtained by near infrared spectroscopy (NIRS) and analyzed. Each specimen was placed on a diffuse reflectance accessory of a NIR spectrometer to obtain the respective spectrum, using ten replicates for each species or sex. All spectra were analyzed by combined methods of multivariate analysis using the technique of crossed validation for the multivariate models. The analysis discriminated the species without significant overlapping among the species spectral patterns. The NIRS also revealed variation in the metabolomic profile of males and females; however, it is still possible to distinguish the species using only males or females or even from mixed sex samples. NIRS technique proved to be a powerful tool to discriminate species both at intra and interspecific levels based on dispersion spectral patterns of individual specimens. Keywords: Biological systems, Liposcelididae, stored product pests, Vibrational spectroscopy

Clinical Features And Laboratory Patterns In A Cohort Of Consecutive Argentinian Patients With Von Willebrand's Disease

Background and Objectives. von Willebrand's disease (vWD) is a bleeding disorder with variable clinical expression. Our aim was to classify patients with vWD and to determine the phenotype in their relatives. Design and Methods. The types and subtypes, blood group frequency and its relevance, bleeding sites, response to the desmopressin (DDAVP) test, transfusion requirements and clinical features in type 1 and 2A families were determined in 1,885 patients. Results. Our findings were: type 1: 91%, type 2A: 3.1%, severe vWD: 1.3%; type 2N: 1.6%; type low intraplatelet: 2.7%; combined 1+2N: 0.3%. Blood group O prevalence was 70.5%. Bleeding and transfusion requirements were not correlated to blood groups. The most frequent symptoms were: ecchymoses-hematomas and epistaxis and, in females over 13 years, also menorrhagia. Normal levels of factor VIII:C were found in 38.4% of the patients. DDAVP was infused in 567 patients with a good response in 80.6%. About 9% of our patients needed transfusion therapy. The diagnosis of von Willebrand’s disease is more likely in subjects belonging to families with type 2A disease than in members of families with type 1 vWD in spite of these being symptomatic. Interpretation and Conclusions. These observations provide a good strategy to identify, classify and treat vWD patients without performing molecular assays.Fil: Woods, Adriana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Meschengieser, S. S.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Blanco, A. N.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Salviu, M. J.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Farias, Cristina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Kempfer, Ana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentin

Antioxidant Therapy in Parkinson's Disease: Insights from Drosophila melanogaster.

Reactive oxygen species (ROS) play an important role as endogenous mediators in several cellular signalling pathways. However, at high concentrations they can also exert deleterious effects by reacting with many macromolecules including DNA, proteins and lipids. The precise balance between ROS production and their removal via numerous enzymatic and nonenzymatic molecules is of fundamental importance for cell survival. Accordingly, many neurodegenerative disorders, including Parkinson's disease (PD), are associated with excessive levels of ROS, which induce oxidative damage. With the aim of coping with the progression of PD, antioxidant compounds are currently receiving increasing attention as potential co-adjuvant molecules in the treatment of these diseases, and many studies have been performed to evaluate the purported protective effects of several antioxidant molecules. In the present review, we present and discuss the relevance of the use of Drosophila melanogaster as an animal model with which to evaluate the therapeutic potential of natural and synthetic antioxidants. The conservation of most of the PD-related genes between humans and D. melanogaster, along with the animal's rapid life cycle and the versatility of genetic tools, makes fruit flies an ideal experimental system for rapid screening of antioxidant-based treatments

Low complexity model to study scale dependence of phytoplankton dynamics in the tropical Pacific

We demonstrate that a simple model based on reaction-diffusion-advection (RDA) equation forced by realistic surface velocities and nutrients is skilled in reproducing the distributions of the surface phytoplankton chlorophyll in the tropical Pacific. We use the low-complexity RDA model to investigate the scale-relationships in the impact of different drivers (turbulent diffusion, mean and eddy advection, primary productivity) on the phytoplankton chlorophyll concentrations. We find that in the 1/4° (~25km) model, advection has a substantial impact on the rate of primary productivity, whilst the turbulent diffusion term has a fairly negligible impact. Turbulent diffusion has an impact on the phytoplankton variability, with the impact being scale-propagated and amplified by the larger scale surface currents. We investigate the impact of a surface nutrient decline and some changes to mesoscale eddy kinetic energy (climate change projections) on the surface phytoplankton concentrations. The RDA model suggests that unless mesoscale eddies radically change, phytoplankton chlorophyll scales sub-linearly with the nutrients, and it is relatively stable with respect to the nutrient concentrations. Furthermore we explore how a white multiplicative Gaussian noise introduced into the RDA model on its resolution scale propagates across spatial scales through the non-linear model dynamics under different sets of phytoplankton drivers. The unifying message of this work is that the low complexity (e.g. RDA) models can be successfully used to realistically model some specific aspects of marine ecosystem dynamics and by using those models one can explore many questions that would be beyond computational affordability of the higher-complexity ecosystem models

Chiral recognition in D-, L-arginine derived polyamidoamino acids and sodium deoxycholate solutions

Nowadays the spontaneous self-organization of a polymer into an ordered structure is a sought-after property of many smart materials, whose applications might range from catalysis1 to drug-delivery2. However, literature regarding the role played by these specific conformations in chiral recognition remains scarce. In this context, polyamidoamino acids (PAACs) are an emerging class of stimuli-responsive bioinspired synthetic polymers able to self-assemble into pH depend conformations.3,4 Arginine based PAACs, named ARGO7, were obtained in water at pH 8-9 from the stepwise polyaddition of L- or D-arginine to N,N\u2019methylenebisacrylamide. Results indicated Mn 8500, PDI 1.4 and Rh of 1.2 nm.3 Molecular dynamics (MD) and circular dichroism (CD) showed ARGO7 folded into a rigid structure, reminiscent of the hairpin conformation, solely driven by the polymer main chain. Due to its ability to self-assemble in solution forming chiral structures, L- and D-ARGO7 may selectively interact with biological components. To assess chiral recognition, sodium deoxycholate (NaDC), one of the components of bile salts, was chosen as a chiral model surface. In aqueous solution, NaDC showed three different pH dependent behaviour: homogeneous solution (pH>8), gel phase (pH 7-8) and aggregation/flocculation (pH<6.5). Notwithstanding the ability of NaDC to self-assemble into different conformations at each pH interval, signs of chiral recognition were found in NaDC gel phase only. Conformational modifications were probed by circular dichroism spectroscopy: both D- and L-ARGO7 changed shape and magnitude of the CD pattern, whereas D,LARGO7 did not modify the CD spectra of NaDC. After 8 days, NaDC compact structure loosened, ended up being fluid and the CD pattern were completely modified due to NaDC and D- or L-ARGO7 interactions. Incoming SANS studies will probably highlight the mechanisms and dynamics of the chiral interactions in these polyelectrolyte-micelle systems. (1) Luo, R.; Zhu, M.; Shen, X.; Li, S. J. Catal. 2015, 331, 49. (2) Qui\uf1ones, J. P.; Peniche, H.; Peniche, C. Polymers. 2018, 10, 3, 235. (3) Manfredi, A.; Mauro, N.; Terenzi, A.; Alongi, J.; Lazzari, F.; Ganazzoli, F.; Raffaini, G.; Ranucci, E.; Ferruti, P. ACS Macro Lett. 2017, 6, 987. (4) Lazzari, F.; Manfredi, A.; Alongi, J.; Mendichi, R.; Ganazzoli, F.; Raffaini, G.; Ferruti, P.; Ranucci, E. Polymers 2018, 10, 1261

Probing chiral interactions between L- and D-arginine-based polymers and sodium deoxycholate solutions

Nowadays the spontaneous self-organization of a polymer into an ordered structure is a soughtafter property of many smart materials, whose applications might range from catalysis [1] to drugdelivery [2]. However, literature regarding the role played by these specific conformations in chiral recognition remains scarce. In this context, polyamidoamino acids (PAACs) are an emerging class of stimuli-responsive bioinspired synthetic polymers able to self-assemble into pH depend conformations [3,4]. PAACs are an off-spring of polyamidoamines (PAAs), a family of polymers obtained by the Michael-type polyaddition of prim-monoamines or sec-diamines with bisacrylamides. The reaction occurs in aqueous solution at pH = 8\u20139 and at room temperature. By using \u3b1-amino acids as monomers, PAACs are obtained. The first example of PAAC was named ARGO7, obtained by the stepwise polyaddition in water of L- or D-arginine to N,N\u2019-methylenebisacrylamide. Results indicated Mn 8500, PDI 1.4 and Rh of 1.2 nm [3]. Molecular dynamics (MD) and circular dichroism (CD) showed ARGO7 folded into a rigid structure, reminiscent of the hairpin conformation, solely driven by the polymer main chain. Due to its ability to self-assemble in solution forming chiral structures, L- and D-ARGO7 may selectively interact with biological components. To assess chiral recognition, sodium deoxycholate (NaDC), one of the components of bile salts, was chosen as a chiral model surface. In aqueous solution, NaDC showed three different pH dependent behaviour: homogeneous solution (pH>8), gel phase (pH 7-8) and aggregation/flocculation (pH<6.5). Notwithstanding the ability of NaDC to self-assemble into different conformations at each pH interval, signs of chiral recognition were found in NaDC gel phase only. Conformational modifications were probed by circular dichroism spectroscopy: both D- and L-ARGO7 changed shape and magnitude of the CD pattern, whereas D,L-ARGO7 did not modify the CD spectra of NaDC. After 8 days, NaDC compact structure loosened, ended up being fluid and the CD pattern were completely modified due to NaDC and D- or L-ARGO7 interactions. Incoming diffusion NMR and SANS studies will probably highlight the mechanisms and dynamics of the chiral interactions in these polyelectrolyte-micelle systems. [1] Luo, R.; Zhu, M.; Shen, X.; Li, S. J. Catal. 2015, 331, 49. [2] Qui\uf1ones, J. P.; Peniche, H.; Peniche, C. Polymers. 2018, 10, 3, 235. [3] Manfredi, A.; Mauro, N.; Terenzi, A.; Alongi, J.; Lazzari, F.; Ganazzoli, F.; Raffaini, G.; Ranucci, E.; Ferruti, P. ACS Macro Lett. 2017, 6, 987. [4] Lazzari, F.; Manfredi, A.; Alongi, J.; Mendichi, R.; Ganazzoli, F.; Raffaini, G.; Ferruti, P.; Ranucci, E. Polymers 2018, 10, 1261

Real-time reconstruction of long-lived particles at LHCb using FPGAs

Finding tracks downstream of the magnet at the earliest LHCb trigger level is not part of the baseline plan of the upgrade trigger, on account of the significant CPU time required to execute the search. Many long-lived particles, such as $K^0_S$ and strange baryons, decay after the vertex track detector, so that their reconstruction efficiency is limited. We present a study of the performance of a future innovative real-time tracking system based on FPGAs, developed within a R\&D effort in the context of the LHCb Upgrade Ib (LHC Run~4), dedicated to the reconstruction of the particles downstream of the magnet in the forward tracking detector (Scintillating Fibre Tracker), that is capable of processing events at the full LHC collision rate of 30 MHz.Comment: ACAT 2019 proceedings. 7 pages, 2 figure

Cellular kinetics of perivascular MSC precursors

Mesenchymal stem/stromal cells (MSCs) and MSC-like multipotent stem/progenitor cells have been widely investigated for regenerative medicine and deemed promising in clinical applications. In order to further improve MSC-based stem cell therapeutics, it is important to understand the cellular kinetics and functional roles of MSCs in the dynamic regenerative processes. However, due to the heterogeneous nature of typical MSC cultures, their native identity and anatomical localization in the body have remained unclear, making it difficult to decipher the existence of distinct cell subsets within the MSC entity. Recent studies have shown that several blood-vessel-derived precursor cell populations, purified by flow cytometry from multiple human organs, give rise to bona fide MSCs, suggesting that the vasculature serves as a systemic reservoir of MSC-like stem/progenitor cells. Using individually purified MSC-like precursor cell subsets, we and other researchers have been able to investigate the differential phenotypes and regenerative capacities of these contributing cellular constituents in the MSC pool. In this review, we will discuss the identification and characterization of perivascular MSC precursors, including pericytes and adventitial cells, and focus on their cellular kinetics: cell adhesion, migration, engraftment, homing, and intercellular cross-talk during tissue repair and regeneration. © 2013 William C. W. Chen et al