Identifying well-connected opinion leaders for informal health promotion: the example of the ASSIST smoking prevention program

Methods used to select opinion leaders for informal behavior change interventions vary, affecting the role they adopt and the outcomes of interventions. The development of successful identification methods requires evidence that these methods achieve their aims. This study explored whether the “whole community” nomination process used in the ASSIST smoking prevention program successfully identified “peer supporters” who were well placed within their school social networks to diffuse an antismoking message to their peers. Data were collected in the United Kingdom during A Stop Smoking in Schools Trial. Behavioral data were provided at baseline and post intervention by all students. Social network data were provided post intervention by students in four control and six intervention schools. Centrality measures calculated using UCINET demonstrate that the ASSIST nomination process successfully identified peer supporters who were more socially connected than others in their year and who had social connections across the entire year group including the program’s target group. The results indicate that three simple questions can identify individuals who are held in high esteem by their year group and who also have the interpersonal networks required of opinion leaders to successfully disseminate smoke-free messages through their social networks. This approach could be used in other informal health promotion initiatives

Bloodstream form trypanosoma brucei depend upon multiple metacaspases associated with RAB11-positive endosomes

Trypanosoma brucei possesses five metacaspase genes. Of these, MCA2 and MCA3 are expressed only in the mammalian bloodstream form of the parasite, whereas MCA5 is expressed also in the insect procyclic form. Triple RNAi analysis showed MCA2, MCA3 and MCA5 to be essential in the bloodstream form, with parasites accumulating pre-cytokinesis. Nevertheless, triple null mutants (Δmca2/3Δmca5) could be isolated after sequential gene deletion. Thereafter, Δmca2/3Δmca5 mutants were found to grow well both in vitro in culture and in vivo in mice. We hypothesise that metacaspases are essential for bloodstream form parasites, but they have overlapping functions and their progressive loss can be compensated for by activation of alternative biochemical pathways. Analysis of Δmca2/3Δmca5 revealed no greater or lesser susceptibility to stresses reported to initiate programmed cell death, such as treatment with prostaglandin D2. The metacaspases were found to colocalise with RAB11, a marker for recycling endosomes. However, variant surface glycoprotein (VSG) recycling processes and the degradation of internalised anti-VSG antibody were found to occur similarly in wild type, Δmca2/3Δmca5 and triple RNAi induced parasites. Thus, the data provide no support for the direct involvement of T. brucei metacaspases in programmed cell death and suggest that the proteins have a function associated with RAB11 vesicles that is independent of known recycling processes of RAB11-positive endosomes

Effects of a developmental adventure on the self-esteem of college students

This study examines the effects of outdoor developmental adventure programming (ODA) on college students’ self-esteem. Although some previous studies have shown that outdoor adventure programming has positive effects on self-esteem, others did not find any effect. A quasi-experimental study was conducted over 5 months, which included two pretests and two posttests to address some limitations of previous studies. A total of 84 participants completed the Questionnaire d’estime de soi hiérarchique, a questionnaire assessing self-esteem on four occasions. The experimental group (outdoor adventure; n = 32) was compared with the control groups (travel, n = 17; soccer, n = 35) using repeated measures of covariance analyses. Positive and significant increases were found for the athletic dimension of self-esteem and for global self-esteem for the experimental group (outdoor adventure) only. The increased benefits provided by the combination of adventure and sports in ODA, over sports-only or traveling-only interventions, underline the importance of developing ODA with college students

An In Vitro Evaluation of Leakage of Two Etch and Rinse and Two Self-Etch Adhesives after Thermocycling

Our experiment evaluated the microleakage in resin composite restorations bonded to dental tissues with different adhesive systems. 40 class V cavities were prepared on the facial and lingual surfaces of each tooth with coronal margins in enamel and apical margins in cementum (root dentin). The teeth were restored with Z100 resin composite bonded with different adhesive systems: Scotchbond Multipurpose (SBMP), a 3-step Etch and Rinse adhesive, Adper Scotchbond 1 XT (SB1), a 2-step Etch and Rinse adhesive, AdheSE One (ADSE-1), a 1-step Self-Etch adhesive, and AdheSE (ADSE), a 2-step Self-Etch adhesive. Teeth were thermocycled and immersed in 50% silver nitrate solution. When both interfaces were considered, SBMP has exhibited significantly less microleakage than other adhesive systems (resp., for SB1, ADSE-1 and ADSE, P = 0.0007, P < 0.0001 and P < 0.0001). When enamel and dentin interfaces were evaluated separately, (1) for the Self-Etch adhesives, microleakage was found greater at enamel than at dentin interfaces (for ADSE, P = 0.024 and for ADSE-1, P < 0.0001); (2) for the Etch and Rinse adhesive systems, there was no significant difference between enamel and dentin interfaces; (3) SBMP was found significantly better than other adhesives both at enamel and dentin interfaces. In our experiment Etch and Rinse adhesives remain better than Self-Etch adhesives at enamel interface. In addition, there was no statistical difference between 1-step (ADSE-1) and 2-step (ADSE) Self-Etch adhesives

Sexual satisfaction during pregrancy

Introduction. Expecting a first child is a challenging period for relationship partners, especially in regard to their sex life. In fact, sexual satisfaction can diminish for most pregnant couples. Objective. This research aimed to explore the associations between attachment insecurity (anxiety and avoidance) and sexual satisfaction through relationship intimacy and partner support among both partners during pregnancy. Method. During the second trimester of pregnancy, 127 first-time parent couples completed online questionnaires assessing romantic attachment, sexual satisfaction, intimacy, and couple support. This study was cross-sectional. Path analyses based on the Actor-Partner Interdependance Model were conducted. Results. Results revealed the presence of indirect associations between attachment avoidance, but not anxiety, and lower sexual satisfaction, via intimacy and couple support, in women and men. Men’s attachment avoidance was also indirectly related to their pregnant partners’ lower sexual satisfaction through these variables. Conclusion. These results highlight the importance of relational processes, namely intimacy and support, in sexual satisfaction during a pregnancy

Positive adaptation in new parents

Abstract : Objective: This study aimed to explore the lived experience of well-satisfied couples (as established by the short form of the Dyadic Adjustment Scale) through the transition to parenthood (TTP) to understand what they perceived has facilitated their relationship adaptation. Background: Most couples experience a decline in relationship satisfaction through the TTP. However, there is important variability in the couples’ experience, and few researchers have examined positive adaptation. Method: Semistructured individual interviews were conducted with both partners of seven firsttime parental couples (N = 14) and then were transcribed and analyzed using interpretative phenomenological analysis. Result: The results showed two interrelated superordinate themes, each including four subordinate themes. Interviewed couples remained satisfied due to the strong foundations of their relationship, namely security, commitment, compassionate love, and intimacy, and due to their effective management of changes together by teaming up, balancing the different spheres of their lives, enjoying and valuing family life together, and communicating. Conclusion: Our findings support the relevance of studying positive couple processes for prevention efforts to ease the transition to parenthood. Implications: Professionals working with expectant and new parents could target relational processes related to strong foundations as well as the partners’ joint management of change to strengthen couple relationships and promote the positive adaptation to parenthood of partners

Characterization of pentraxin 3 in the horse and its expression in airways

The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses’ airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, Western blotting and immuno-histochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: “spliced” (32 kDa) and “full length” (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O.-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives

Friction properties of fluorinated carbons

In boundary lubrication regime, friction reduction and antiwear processes are associated to the presence of additives in the lubricating oils or greases. These processes are due to the formation of protective tribofilms resulting from chemical reactions between the additives and the sliding surfaces, in the physico-chemical conditions of the sliding contact. Conventional antiwear additives mainly consist of transition metal organo phosphate or thiophosphates which present a remarkable efficiency in the case of contacts between ferrous alloys. In the case of non reacting surfaces, these additives become inactive. Recently developped lubrication strategies consist in the use of dispersion in oils of nano additives able to build the protective tribofilm in the sliding contact without reaction with the surfaces. Carbon fluorinated phases, due to their lamellar structure and their high chemical stability even at relatively high temperature (400°C) represent interesting candidates as lubricant nano-additives subjected to present friction reduction, anti wear and anti corrosion actions. This work presents the tribologic behaviour of some carbon fluorinated derivatives such as graphite fluorides, fluorinated carbon nanofibers, fluorinated carbon nanodiscs and fluorinated carbon blacks. The influence, on the tribologic performances, of the structure of the initial carbon phases, of the fluorination rate (0<F/C<1) and the structure of the fluorinated compounds is discussed

Microleakage after Thermocycling of Three Self-Etch Adhesives under Resin-Modified Glass-Ionomer Cement Restorations

This study was designed to evaluate microleakage that appeared on Resin-Modified Glass-Ionomer Cement (RMGIC) restorations. Sixty class V cavities (h × w × l = 2 mm × 2 mm × 3 mm) were cut on thirty extracted third molars, which were randomly allocated to three experimental groups. All the buccal cavities were pretreated with polyacrylic acid, whereas the lingual cavities were treated with three one-step Self-Etch adhesives, respectively, Xeno III (Dentsply Detrey GmbH, Konstanz, Germany), iBond exp (Heraeus Kulzer gmbH & Co. KG, Hanau, Germany), and Adper Prompt-L-Pop (3M ESPE AG, Dental products Seefeld, Germany). All cavities were completely filled with RMGIC, teeth were thermocycled for 800 cycles, and leakage was evaluated. Results were expressed as means ± standard deviations (SDs). Microleakage scores were analysed by means of generalized linear mixed models (GLMMs) assuming an ordinal logistic link function. All results were considered to be significant at the 5% critical level (P < .05). The results showed that bonding RMGIC to dentin with a Self-Etch adhesive rather than using polyacrylic acid did not influence microleakage scores (P = .091), except for one tested Self-Etch adhesive, namely, Xeno III (P < .0001). Nevertheless, our results did not show any significant difference between the three tested Self-Etch adhesive systems. In conclusion, the pretreatment of dentin with Self-Etch adhesive system, before RMGIC filling, seems to be an alternative to the conventional Dentin Conditioner for the clinicians as suggested by our results (thermocycling) and others (microtensile tests)

Classroom anxiety and enjoyment in CLIL and non-CLIL: Does the target language matter?

This study investigates pupils’ anxiety and enjoyment in the classroom when learning a second or foreign language. The particularity of this study lies in the comparison of two target languages (English and Dutch) in two educational contexts (CLIL and non-CLIL) at different instruction levels (primary and secondary education). While most research on content and language integrated learning (CLIL) focuses on English as a target language, the Belgian context calls for a comparison with the language of the “other” community, in this case Dutch. Data were collected from 896 pupils in French-speaking Belgium through a self-report questionnaire measuring pupils’ anxiety and enjoyment in the classroom, along with background characteristics. Results indicate that while CLIL pupils experience significantly less anxiety than their non-CLIL counterparts, English learners report significantly less anxiety and more enjoyment than Dutch learners. This suggests an important role of the target language for emotional engagement in the classroom and calls for further investigation into the role of target language perceptions. Finally, the interactions with instruction level reveal that while primary school pupils report stronger emotions, the effects of CLIL and English are much larger at secondary level