Investigation of oral and general health status and IL-1β gene polymorphism as risk factors for oral mucositis in hematopoietic stem cell transplantation patients

The aim of the present study was to analyze the relationship of OM with possible risk factors such as oral health condition, immunological status and IL-1β profile in patients submitted to hematopoietic stem cell transplantation (HSCT). Fifty-four individuals submitted to HSCT were included. All patients received previous dental treatment and photobiomodulation (PBM) as the institutional OM preventive protocol. OM scores, immune status, and IL-1β levels were determined during the conditioning period and at D+3 and D+8 after HSC infusion. IL-1β gene polymorphism was also analyzed during conditioning. Possible associations of OM with risk factors were analyzed using conditional Fisher’s exact test. OM was observed in 34 patients (62.9%) classified as Grade 1 (13 patients/24.1%), Grade 2 (14 patients/25.9%), Grade 3 (3 patients/5.5%), and Grade 4 (4 patients/7.4%). Allogeneic HSCT individuals exhibited a higher OM grade than autologous subjects. Moreover, an association was observed between severe OM and severe gingivitis (p = 0.01), neutropenia (p = 0.03), and leukopenia (p = 0.04). A significant association between OM and lower IL-1β levels was detected at three time points, i.e., conditioning (p = 0.048), D+3 (p = 0.01), and D+8 (p = 0.005). The results showed that IL-1β gene polymorphism was not associated with OM. Our study provided important insights into the scope of OM risk factors in the setting of HSCT. Patients submitted to HSCT with severe gingivitis prior to chemotherapy and with severe neutropenia and leukopenia exhibited a higher OM grade. Further investigation will be necessary to better understand the exact role of IL-1β in the context of OM pathobiology and to validate cytokine analysis in larger cohorts

Acinic cell carcinoma of the oral and maxillofacial region: an international multicenter study

Abstract The aim of this study was to describe the prevalence, clinicopathological, and prognostic features of acinic cell carcinoma (AciCC) of the oral and maxillofacial region. AciCC cases were retrospectively retrieved from 11 pathology centers of three different countries. Medical records were examined to extract demographic, clinical, pathologic, and follow-up information. A total of 75 cases were included. Females (65.33%) with a mean age of 45.51 years were mostly affected. The lesions usually presented as an asymptomatic (64.28%) nodule (95.66%) in the parotid gland (70.68%). The association of two histopathological patterns was the most common finding (48.93%) and the tumors presented mainly conventional histopathological grades (86.11%). Surgical treatment was performed in the majority of the cases (59.19%). Local recurrence was observed in 20% of the informed cases, regional metastasis in 30.43%, and distant metastasis in 12.50%. The statistical analysis showed that the cases with a solid histopathological pattern (p=0.01), high-grade transformation (p=0.008), recurrence (p=0.007), and regional metastasis (p=0.03) were associated with poor survival. In conclusion, high histopathological transformation, presence of nodal metastasis, and recurrence were prognostic factors for AciCC of the oral and maxillofacial region

Expression of CHK2, γH2AX and TP53 proteins in smokers and non-smokers oral squamous cell carcinoma

Submitted by Lauren Schuch ([email protected]) on 2019-12-04T17:43:14Z No. of bitstreams: 1 DISSERTAÇAO FINAL 3-12-19.pdf: 14000755 bytes, checksum: 402e9e04d9b50c56f371f9448c9d276e (MD5)Rejected by Jane Campos ([email protected]), reason: Prezado(a), Conforme as “Diretrizes para Normalização de Trabalhos Acadêmicos da UFMG” detectamos que em seu trabalho não consta: Ficha Catalográfica e a Ata de defesa. Considerando a visibilidade nacional e internacional do RI/UFMG, os trabalhos disponibilizados devem seguir padrões e protocolos de integração qualificados e normalizados. Diante disso, gentileza executar as inserções devidas acessando o link abaixo. https://repositorio.ufmg.br/custom/instructions.jsp Solicitamos que o termo de autorização seja preenchido, assinado e encaminhado à secretaria do seu curso. Aguardamos nova submissão. Atenciosamente, Equipe do Repositório. on 2019-12-06T16:40:22Z (GMT)Submitted by Lauren Schuch ([email protected]) on 2019-12-10T23:15:02Z No. of bitstreams: 1 dissertaçao completa.pdf: 14461602 bytes, checksum: 017a356c73a3feb87ff675fde56271e4 (MD5)Approved for entry into archive by Eliane Andrade ([email protected]) on 2019-12-17T13:17:13Z (GMT) No. of bitstreams: 1 dissertaçao completa.pdf: 14461602 bytes, checksum: 017a356c73a3feb87ff675fde56271e4 (MD5)Made available in DSpace on 2019-12-17T18:00:02Z (GMT). No. of bitstreams: 1 dissertaçao completa.pdf: 14461602 bytes, checksum: 017a356c73a3feb87ff675fde56271e4 (MD5) Previous issue date: 2019-11-20CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO câncer de boca corresponde a cerca de 4% das doenças neoplásicas, e o carcinoma de células escamosas representa o tipo mais frequente, englobando cerca de 90 a 95% dos casos. O cigarro é o principal fator etiológico para o câncer de boca, causando danos ao DNA e mutações que se não reparadas levam ao surgimento de lesões. O dano ao DNA associado ao cigarro tem sido estudado em diferentes tipos de câncer, mas ainda pouco explorado em relação ao câncer de boca. Checkpoint kinase 2 (CHK2) e P53 são proteínas que estão envolvidas no processo de checagem do ciclo celular, sendo responsáveis pelo reparo ao dano ao DNA. A proteína H2AX é uma histona nuclear que sofre fosforilação em resposta aos danos ao DNA, principalmente às quebras da dupla cadeia. O objetivo deste estudo foi avaliar a resposta a danos no DNA através da expressão de CHK2, γH2AX e TP53 entre fumantes e não fumantes com carcinoma de células escamosas de boca (CCEB). Além disso, foram analisadas associações entre imuno-expressão das proteínas estudadas, dados clínico-patológicos e classificação histopatológica. Foram incluídos 35 indivíduos (18 não fumantes e 17 fumantes) com CCEB de língua e soalho bucal. Reação imuno-histoquímica foi realizada para γH2AX para identificação de quebras de fita dupla, CHK2 e TP53 para avaliação da indução de parada do ciclo celular. Análises descritivas e estatísticas foram realizadas. A pesquisa foi composta por 22 homens (62,8%) e 13 mulheres (37,2%), com idade média de 63,9 anos. Entre os não fumantes, 50% apresentaram tumores bem diferenciados, enquanto que fumantes mostraram maior número em moderadamente diferenciados e pouco diferenciados (35,3% cada). No geral, 31 (88,6%) casos foram positivos para CHK2, 27 (77,2%) foram positivos para γH2AX e 23 (65,7%) foram positivos para TP53. Não foi observada associação entre essas proteínas com hábitos de fumar e não fumar (p> 0,05). Semelhanças entre os padrões imuno-histoquímicos de CHK2, γH2AX e TP53 em fumantes e não fumantes com CCEB foram observadas neste estudo, assim como entre os parâmetros clínico-patológicos. De forma geral, os resultados indicaram expressão positiva para essas proteínas no CCEB. Este estudo fornece informaçaões sobre o dano ao DNA na carcinogênese oral.Oral cancer accounts for about 4% of neoplastic diseases, and squamous cell carcinoma is the most common type, accounting for about 90 to 95% of cases. Cigarette smoking is the main etiological factor for oral cancer, causing DNA damage and mutations that, if not repaired, lead to lesions. Cigarette-associated DNA damage has been studied in different cancers, but is still poorly explored in relation to oral cancer. Checkpoint kinase 2 (CHK2) and P53 are proteins that are involved in the cell cycle checking process and are responsible for repairing DNA damage. The H2AX protein is a nuclear histone that undergoes phosphorylation in response to DNA damage, especially double strand breaks. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), γH2A histone family member X (γH2AX) and TP53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). In addition, associations amongst immunoexpression of studied proteins, clinicopathologic data and histopathological grading were analyzed. Thirty-five individuals (18 nonsmokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry was carried out for γH2AX for identification of double-strand breaks, CHK2 and P53 for evaluation of the induction of cell cycle arrest. Descriptive and statistical analyses were performed. The survey consisted of 22 males (62.8%) and 13 females (37.2%), with a mean age of 63.9 years. Fifty percent of non-smokers OSCC were well-differentiated tumors, whereas for smokers, OSCC were moderately differentiated and poorly differentiate tumors, equally (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.2%) were γH2AX-positive and 23 (65.7%) were TP53-positive. No association among these proteins with smoking and non-smoking habits was observed (p>0.05). Similarities in the CHK2, γH2AX and P53 immunohistochemical staining pattern were observed between smokers and non-smokers with OSCC in this survey, and the immunoexpression was not associated with clinicopathologic parameters. Overall, the results indicated consistent expression of these proteins in OSCC. This study provides information about the DNA damage in oral carcinogenesis

A Brazilian multicentre study of 2,497 isolated cases of odontogenic keratocysts

We present the frequency of cases of isolated odontogenic keratocysts submitted to microscopic examination at 10 Brazilian referral centres in Oral and Maxillofacial Pathology. In a retrospective (1953–2017) analysis, data on clinicoradiographic features and treatment of these lesions were collected and analysed descriptively. Among the 258,867 cases retrieved, 2,497 (0.96%) were isolated odontogenic keratocysts. In summary, an overview of individuals affected with isolated odontogenic keratocysts is reported herein. This lesion showed predilection for the posterior mandible of young adult men263711715CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES305493/2018‐3; 455644/2018‐100

A terapia com teriparatida é eficaz para a osteonecrose dos maxilares associada a medicamentos? Uma revisão sistemática e metanálise

O objetivo da presente pesquisa é combinar os dados disponíveis publicados na literatura sobre casos de OMAM tratados com TPTD em uma revisão sistemática das características demográficas e clínicas dos indivíduos acometidos por esta condição

Effects of tobacco on the DNA of smokers and non-smokers affected by OSCC: systematic review and meta-analysis

Abstract Scientific evidence about genetic and molecular changes in oral squamous cell carcinoma (OSCC) among smokers and non-smokers is inconclusive. This systematic review and meta-analysis assessed the effects of tobacco on the DNA of individuals with OSCC based on protein mutations. Electronic searches were conducted on PubMed, Ovid, Web of Science, and Scopus to identify observational studies published up to January/2022. The Joanna Briggs Institute tool was used for the critical appraisal of studies. The certainty of the evidence was evaluated. Twenty-three studies assessing 4,060 individuals (2,967 smokers vs. 1,093 non-smokers) were included in this review. Fifteen groups of proteins/genes were investigated. Analysis of the quality of articles revealed low risk of bias in most studies. The certainty of the evidence was very low. The meta-analysis confirmed no significant difference between smokers and non-smokers with respect to damage to GSTM1 (OR: 0.60; 95%CI: 0.30–1.18), GSTT1 (OR: 1.18; 95%CI:0.49–2.83), hydrolase proteins (Ku70 and Ku80) (OR: 0.74; 95%CI: 0.18–3.05), and transferase proteins (GSTM1, GSTT1, GSTM3) (OR: 0.74; 95%CI: 0.47–1.18). Most of the studies included showed that smokers are more likely to exhibit genetic instability. However, the meta-analysis revealed that smokers do not necessarily have more genetic alterations in the DNA than non-smokers

Oral lymphangioma‑like Kaposi sarcoma : a Brazilian case report in a scenario of a still high number of HIV infections

We present a challenging case of HIV-related lymphangioma-like Kaposi sarcoma (LLKS) afecting the oral cavity. A 54-year-old Brazilian male patient was referred to our center complaining of bleeding lesions afecting the oral cavity for 2 months. Interestingly, these oral lesions were the frst clinical manifestation of HIV infection. Clinically, multiple erythematous nodular and patch lesions were observed. An incisional biopsy was performed, revealing numerous microscopic angled and irregular vascular channels lined with fattened endothelial cells. More cellularized and solid areas consisting of more fusiform cells with little pleomorphism and with slit-like vascular channels were noted. Based on immunoreactivity for CD31, CD34, D2-40, and HHV-8, the fnal diagnosis was oral LLKS. Highly active antiretroviral therapy (HAART) was initiated with dolutegravir, tenofovir, and lamivudine. During follow-up, the patient showed KS metastases to other sites and a chemotherapeutic protocol was initiated. Regression of the oral lesion was clearly noted by the clinicians 1 year after the KS diagnosis. Dentists should be able to recognize systemic diseases that afect the oral cavity such as KS in order to make an early diagnosis of its oral manifestations and to implement efective therapeutic measures to ensure a better prognosis