Inherent variation of functional traits in winter and summer leaves of Mediterranean seasonal dimorphic species. Evidence of a 'within leaf cohort' spectrum

The covariation pattern among leaf functional traits involved in resource acquisition has been successfully provided by the leaf economic spectrum (LES). Nevertheless, some aspects such as how the leaf trait variation sources affect LES predictions are still little investigated. Accordingly, the aim of this paper was to test whether leaf trait variations within different leaf cohorts could alter LES. Improving this knowledge can extend the potential of trait-based approaches in simulating future climate effects on ecosystems. A database on leaf morphological and physiological traits from different leaf cohorts of Cistus spp. was built by collecting data from literature. These species are seasonal dimorphic shrubs with two well-defined leaf cohorts during a year: summer leaves (SL) and winter leaves (WL). Traits included: leaf mass area (LMA), leaf thickness (LT), leaf tissue density (LTD), net photosynthetic rate on area (Aa) and mass (Am) base, nitrogen content on area (Na) and mass (Nm) base. The obtained patterns were analysed by standardized major axis regression and then compared with the global spectrum of evergreens and deciduous species. Climatic variable effect on leaf traits was also tested. Winter leaves and SL showed a great inherent variability for all the considered traits. Nevertheless, some relationships differed in terms of slopes or intercepts between SL and WL and between leaf cohorts and the global spectrum of evergreens and deciduous. Moreover, climatic variables differently affected leaf traits in SL and WL. The results show the existence of a 'within leaf cohort' spectrum, providing the first evidence on the role of leaf cohorts as LES source of variation. In fact, WL showed a high return strategy as they tended to maximize, in a short time, resource acquisition with a lower dry mass investment, while SL were characterized by a low return strategy

Carbon sequestration and noise attenuation provided by hedges in Rome: the contribution of hedge traits in decreasing pollution levels

AbstractHedges are ubiquitous green elements in many European cities. The selection of hedge types characterized by different traits can be suggested for urban greening projects to decrease pollution levels. At this end, carbon dioxide (CO2) sequestration and noise attenuation capability were analyzed in the following hedge types: Laurus nobilis, Nerium oleander, Pittosporum tobira and Pyracantha coccinea, largely used as green infrastructure in Rome (Italy). Representative hedges for each species were selected from high level traffic streets in the city centre (P sites). Traffic density (TD) was monitored simultaneously with CO2 concentration and noise level (N) in each of the considered P sites. The monthly CO2 sequestration capability (MSC) was calculated multiplying the total photosynthesis per hedge by the total photosynthetic activity time (in hours) per month. The multiple regression analysis predicted noise attenuation (ΔN) by a linear combination of total leaf area (TLA), total leaf density (TLD) and leaf mass area (LMA) of the considered hedge types. All the considered species, being evergreens, were active all year long, including winter, when CO2 emissions from road transport peaked. Nevertheless, among the considered hedge types, P. tobira and L. nobilis were the most efficient species in both MSC (31.6±2.8 and 25.4±2.4 kg CO2 month–1, respectively) and ΔN (15±1%, mean value). The results give insight on the use of hedges to mitigate pollution effects. Moreover, this method can be used to monitor hedge contribution to air quality, in relation to various elements in the city (i.e. traffic density, new cars produced, application of management projects, local laws). These results might be available for projects based on the use of vegetation in order to improve environmental quality in urban areas

Short-term physiological plasticity.Trade-off between drought and recovery responses in three Mediterranean cistus species

Short-term physiological plasticity allows plants to thrive in highly variable environments such as the Mediterranean ecosystems. In such context, plants that maximize physiological performance under favorable conditions, such as Cistus spp., are generally reported to have a great cost in terms of plasticity (i.e., a high short-term physiological plasticity) due to the severe reduction of physiological performance when stress factors occur. However, Cistus spp. also show a noticeable resilience ability in response to stress factors. We hypothesized that in Cistus species the short-term physiological response to stress and that to subsequent recovery can show a positive trade-off to offset the costs of the photosynthetic decline under drought. Gas exchange, chlorophyll fluorescence, and water relations were measured in C. salvifolius, C. monspeliensis, and C. creticus subsp. eriocephalus during an imposed experimental drought and subsequent recovery. Plants were grown outdoor in common garden conditions from seeds of different provenances. The short-term physiological response to stress and that to recovery were quantified via phenotypic plasticity index (PIstress and PIrecovery, respectively). A linear regression analysis was used to identify the hypothesized trade-off PIstress-PIrecovery. Accordingly, we found a positive trade-off between PIstress and PIrecovery, which was consistent across species and provenances. This result contributes in explaining the profit, more than the cost, of a higher physiological plasticity in response to short-term stress imposition for Cistus spp because the costs of a higher PIstress are payed back by an as much higher PIrecovery. The absence of leaf shedding during short-term drought supports this view. The trade-off well described the relative variations of gas exchange and water relation parameters. Moreover, the results were in accordance with the ecology of this species and provide the first evidence of a consistent trade-off between the short-term physiological responses to drought and recovery phases in Mediterranean species

Field host range of Apanteles opuntiarum (Hymenoptera: Braconidae) in Argentina, a potential biocontrol agent of Cactoblastis cactorum (Lepidoptera: Pyralidae) in North America

Field exploration in Argentina for cactophagous lepidopteran hosts parasitized by the recently described braconid parasitoid Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) revealed a host range restricted to Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae) and C. doddi. Field collections of cactophagous larvae parasitized by the congeneric and sympatric parasitoid A. alexanderi included the host species C. bucyrus, Sigelgaita nr. chilensis, Tucumania sp., Tucumania tapiacola and Salambona sp. If a narrow host range for A.opuntiarum is confirmed in quarantine with North American cactus-feeding species, then this parasitoid could be released as a biological control agent for C. cactorum with little or no risk to non-target species.Las exploraciones de campo en Argentina de larvas hospedadoras cactófagas parasitadas por Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae), un bracónido recientemente descripto, revelaron un espectro de hospedadores restringido a Cactoblastis cac­torum (Berg) (Lepidoptera: Pyralidae) y C. doddi. Las colecciones de campo de larvas cactófagas parasitadas por la especie congenérica y simpátrica A. alexanderi identificaron a C. bucyrus, Sigelgaita nr. chi­lensis, Tucumania sp., Tucumania tapiacola y Salambona sp. como es­pecies hospedadoras. Si se confirma en cuarentena el estrecho rango de hospedadores de A. opuntiarum sobre especies norteamericanas que se alimentan de cactus, podría ser liberado como agente de con­trol biológico de C. cactorum, con bajo riesgo o ninguno a especies no blanco.Fil: Varone, Laura. Fundación para el Estudio de Especies Invasivas; ArgentinaFil: Logarzo, Guillermo Alejandro. Fundación para el Estudio de Especies Invasivas; ArgentinaFil: Martinez, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales ; ArgentinaFil: Navarro, Fernando. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; ArgentinaFil: Carpenter, James E.. United States Department of Agriculture; Estados UnidosFil: Hight, Stephen D.. United States Department of Agriculture; Estados Unido

Geographical Range and Laboratory Studies on Apanteles opuntiarum (Hymenoptera: Braconidae) in Argentina, a Candidate for Biological Control of Cactoblastis cactorum (Lepidoptera: Pyralidae) in North America

La polilla de la tuna, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), es una plaga que amenaza las Opuntia spp. nativas de América del Norte. Se han implementado varias estrategias para su control, erradicándola de México pero no de los Estados Unidos, donde ha continuado incrementando su distribución geográfica con éxito. Por lo tanto, se propone un enfoque integrado que incluya al control biológico para regular las poblaciones de C. cactorum en América del Norte. Se realizaron relevamientos de campo del parasitoide recientemente descrito, Apanteles opuntiarum Martínez & Berta, dentro del área de distribución nativa de C. cactorum en Argentina, y se realizaron estudios de laboratorio para desarrollar un protocolo de cría del parasitoide. Apanteles opuntiarum fue el parasitoide más común de C. cactorum y sus distribuciones en el campo coincidieron ampliamente. En el laboratorio, el éxito reproductivo del parasitoide se maximizó cuando una o dos avispas estuvieron expuestas a 30 larvas dentro de un recipiente de 500 ml. Hembras criadas en laboratorio fueron menos exitosas parasitando hospedadores que hembras provenientes del campo. A pesar del éxito logrado en la cría de los parasitoides, obtuvimos una proporción de sexos sesgada hacia machos en los experimentos. Como este sesgo podría estar relacionado a la presencia del parásito reproductivo Wolbachia, tanto la colonia de laboratorio como individuos provenientes del campo fueron analizados y poseían Wolbachia. El presente estudio proporcionó información útil de campo y laboratorio para (1) estandarizar una técnica de cría en laboratorio; (2) realizar estudios de especificidad de hospedadores en condiciones de cuarentena; y (3) seleccionar las poblaciones de parasitoides que mejor coinciden con el clima de las áreas invadidas por C. cactorum en América del Norte.The cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), is a pest that threatens native Opuntia spp. in North America. Control tactics developed and implemented against this invasive pest successfully eradicated the moth in Mexico and on barrier islands in the United States. However, with the cancellation of the regional management program in the United States, no control tactics are being implemented to mitigate the expansion of the moth's geographical range. Hence, an integrated approach including biological control is proposed to regulate the population of C. cactorum in North America. Field surveys of the recently described parasitoid, Apanteles opuntiarum Martínez & Berta, were carried out within the C. cactorum native range in Argentina, and laboratory studies were conducted to develop a parasitoid rearing protocol. Apanteles opuntiarum was the most common parasitoid of C. cactorum and their field distributions were similar. In the laboratory, the parasitoid's reproductive success was maximized when one or two female wasps were exposed to 30 host larvae within a 500 ml container. Laboratory reared females were less successful at parasitizing hosts than field collected females. In spite of the success achieved with laboratory rearing, male bias was observed throughout the experiments. Because this bias might be related to the presence of the reproductive parasite Wolbachia, both laboratory colony and field collected individuals were screened and Wolbachia was detected. This study provides useful field and laboratory information on (1) laboratory rearing techniques for A. opuntiarum; (2) developing host specificity test protocols for studies under quarantine conditions; and (3) selecting parasitoid populations that best match the climatic conditions present in the C. cactorum invaded areas of North America.Fil: Mengoni Goñalons, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Varone, Laura. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Logarzo, Guillermo Alejandro. Fundación para el Estudio de Especies Invasivas; ArgentinaFil: Guala, Mariel. Fundación para el Estudio de Especies Invasivas; ArgentinaFil: Rodriguero, Marcela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Hight, Stephen D.. United States Department Of Agriculture. Agriculture Research Service; Estados UnidosFil: Carpenter, James E.. United States Department Of Agriculture. Agriculture Research Service; Estados Unido

A protocol for health assessment of cacti populations: A case study from Northwestern Argentina

Argentina is considered megadiverse for Cactaceae, and Jujuy province, with a high proportion of cacti endemism, is a critical region for their preservation. Cacti deterioration is mainly associated with habitat degradation, agricultural frontier advance, urbanization, illegal collection and trade. Of the three species of columnar cacti within Trichocereus genus that are present, Trichocereus atacamensis represents a valuable species for local communities. In the last decades, detrimental effects on T. atacamensis populations increased despite the reduction in the use of timber for construction or handicrafts. Knowledge on the health of cacti populations is fundamental for conservation and resource management, therefore the objective of the present study was to develop a health status classification criterion for cacti in Quebrada de Humahuaca, Jujuy. A generic method was elaborated to assess the health of cacti populations to facilitate and speed-up the decision-making process for conservation purposes. The evaluation of factors involved in the deterioration is very complex. We designed a method considering aspects that the local residents identified as the most relevant: a) presence of air plant (genus Tillandsia), b) larvae of Cactoblastis bucyrus and c) signs of rot. Band transects were sampled in patches of T. atacamensis, surveying these aspects for each individual. A Multivariate Regression Tree analysis (MRT) was conducted. According to this clustering, an accessible assessment protocol with a 5-level health status was established to classify the cactus as optimal, suboptimal, compromised, vulnerable or critical. The general health status of the patches was highly variable. This MRT analysis is potentially applicable to any columnar cacti population and to establish the basis of a decision protocol for health status assessment. It is evident that the situation of T. atacamensis transcends as a local problem and exceeds the presence of pathogens suggesting a multifactorial causality that needs special attention.Fil: Barbarich, María Florencia. Universidad Nacional de Jujuy. Instituto de Investigaciones Sobre Naturaleza y Sociedad "Rodolfo Kusch". Laboratorio de Arquitecturas Andinas y Construcción Con Tierra; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Otegui, Florencia Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Saint Esteven, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Soto, Ignacio Maria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Varone, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para el Estudio de Especies Invasivas; Argentin

Carbon Dioxide (CO2) Sequestration and Air Temperature Amelioration Provided by Urban Parks in Rome

Abstract Urban landscapes are rapidly expanding globally and transforming the structure and function of urban areas, thereby influencing the quality of life. Cities account for more than 70% of the energy related to global greenhouse gases, which is expected to rise up to 76% by 2030. Taking into account that over 50% of the world's population lives in cities and more than two thirds are expected by 2050, the problem of mitigating the atmospheric CO 2 concentration is considerable. The urban areas covered by parks, gardens, tree-lined avenues, sport fields, and hedges are important sinks for carbon dioxide (CO 2 ) by storing carbon through photosynthesis to form plant biomass. Despite plant CO 2 sequestration is an important ecosystem service, the relationship between urban park vegetation and CO 2 emission reduction is not completely clarified. In this context, the main objective of our research was to evaluate the role of urban park vegetation in improving air quality in Rome in terms of CO 2 concentration and air temperature. In particular, we analyzed the relationship among the different vegetation types, size and position of an historical urban park in Rome. Moreover, since the presence of buildings within urban parks determines CO 2 emissions closely related to their purpose of use, it is important to evaluate their impact in order to set instruments for their retrofit, considering the necessity of a compromise among the energy audit, the use of renewable energy systems and preservation of cultural heritage

Stem cells and COVID-19: are the human amniotic cells a new hope for therapies against the SARS-CoV-2 virus?

A new coronavirus respiratory disease (COVID-19) caused by the SARS-CoV-2 virus, surprised the entire world, producing social, economic, and health problems. The COVID-19 triggers a lung infection with a multiple proinflammatory cytokine storm in severe patients. Without effective and safe treatments, COVID-19 has killed thousands of people, becoming a pandemic. Stem cells has been suggested as a therapy for lung related diseases. In particular, mesenchymal stem cells (MSCs) have been successfully tested in some clinical trials in patients with COVID-19. The encouraging results positioned MSCs as a possible cell therapy for COVID-19. The amniotic membrane from human placenta at term is a valuable stem cell source, including human amniotic epithelial cells (hAECs) and human mesenchymal stromal cells (hAMSCs). Interestingly, amnion cells have immunoregulatory, regenerative, and anti-inflammatory properties. Moreover, hAECs and hAMSCs have been used both in preclinical studies and in clinical trials against respiratory diseases. They have reduced the inflammatory response and restored the pulmonary tissue architecture in lung-injury in vivo models. Here, we review the existing data about the stem cells use for COVID-19 treatment, including the ongoing clinical trials. We also consider the non-cellular therapies that are being applied. Finally, we discuss the human amniotic membrane cells use in patients who suffer from immune/inflammatory lung diseases and hypothesize their possible use as a successful treatment against COVID-19.Fil: Riedel, Rodrigo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pérez Pérez, Antonio. Universidad de Sevilla; EspañaFil: Sánchez Margalet, Victor S. Universidad de Sevilla; EspañaFil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin

Ecological niche modeling to calculate ideal sites to introduce a natural enemy: the case of apanteles opuntiarum (hymenoptera: Braconidae) to control cactoblastis cactorum (lepidoptera: Pyralidae) in North America

The cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), is an invasive species in North America where it threatens Opuntia native populations. The insect is expanding its distribution along the United States Gulf Coast. In the search for alternative strategies to reduce its impact, the introduction of a natural enemy, Apanteles opuntiarum Martínez and Berta (Hymenoptera: Braconidae), is being pursued as a biological control option. To identify promising areas to intentionally introduce A. opuntiarum for the control of C. cactorum, we estimated the overlap of fundamental ecological niches of the two species to predict their common geographic distributions using the BAM diagram. Models were based on native distributional data for both species, 19 bioclimatic variables, and the Maxent algorithm to calculate the environmental suitability of both species in North America. The environmental suitability of C. cactorum in North America was projected from Florida to Texas (United States) along the Gulf coastal areas, reaching Mexico in northern regions. Apanteles opuntiarum environmental suitability showed a substantial similarity with the calculations for C. cactorum in the United States. Intentional introductions of A. opuntiarum in the actual distribution areas of the cactus moth are predicted to be successful; A. opuntiarum will find its host in an environment conducive to its survival and dispersal.Fil: Pérez De la O, Nidia Bélgica. Universidad Autónoma de Chiapas. Facultad de Ciencias Agrícolas; MéxicoFil: Espinosa Zaragoza, Saúl. Universidad Autónoma de Chiapas. Facultad de Ciencias Agrícolas; MéxicoFil: López Martínez, Víctor. Universidad Autónoma del Estado de Morelos; MéxicoFil: Hight, Stephen D.. US Department of Agriculture. Agricultural Research Service; Estados UnidosFil: Varone, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para el Estudio de Especies Invasivas; Argentin

Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha

The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology.Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Pérez Pérez, Antonio. Hospital Universitario Virgen Macarena; EspañaFil: Dueñas, José L.. Hospital Universitario Virgen Macarena; EspañaFil: Calvo, Juan Carlos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sánchez Margalet, Víctor. Hospital Universitario Virgen Macarena; EspañaFil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentin