185 research outputs found
Iniciativas para la integración, difusión, preservación e intercambio de datos
Sumario:
¿Qué puede aportar el sistema científico a la mejora del proceso de toma de decisiones de los responsables?
Situación de los datos
Algunas actividades
Nueva modalidad de trabajo
Nuevo escenario
Características propias de la información en un observatorio
Objetivo general del observatorio
Iniciativas
Calidad del dato en repositorios temáticos
Beneficios de los datos abiertos
Políticas y acciones para gestionar los datos científicosInfraestructura de Datos Espaciales de la República Argentina (IDERA)Facultad de Ciencias Astronómicas y Geofísica
Abundance of Planktonic Virus-Like Particles in Lake Erie Subsurface Waters
Author Institution: Department of Biological Sciences, Kent State University - Trumbull Campus ; Department of Biological Sciences and Water Resources Research Institute, Kent State UniversityAbundance of virus-like particles (VLP) was determined in Lake Erie subsurface water. The relationship between VLP and the bacterial and phytoplankton communities were investigated. Viral and bacterial numbers were determined using nucleic acid stains and epifluorescent microscopy. Phytoplankton abundance was estimated by chlorophylls extraction. Viral abundance averaged 1.05 x 106 VLP/ml and the ratio of viral to bacterial number was less than 1.0 across most sampling sites and dates. Viral abundance was not correlated with either bacterial abundance or chlorophyll a concentration. Viral abundance was found to be most similar to other Great Lakes and marine systems and dissimilar to other freshwater systems
Effect of modeled reduced gravity conditions on bacterial morphology and physiology
<p>Abstract</p> <p>Background</p> <p>Bacterial phenotypes result from responses to environmental conditions under which these organisms grow; reduced gravity has been demonstrated in many studies as an environmental condition that profoundly influences microorganisms. In this study, we focused on low-shear stress, modeled reduced gravity (MRG) conditions and examined, for <it>Escherichia coli and Staphlyococcus aureus</it>, a suite of bacterial responses (including total protein concentrations, biovolume, membrane potential and membrane integrity) in rich and dilute media and at exponential and stationary phases for growth. The parameters selected have not been studied in <it>E. coli </it>and <it>S. aureus </it>under MRG conditions and provide critical information about bacterial viability and potential for population growth.</p> <p>Results</p> <p>With the exception of <it>S. aureus </it>in dilute Luria Bertani (LB) broth, specific growth rates (based on optical density) of the bacteria were not significantly different between normal gravity (NG) and MRG conditions. However, significantly higher bacterial yields were observed for both bacteria under MRG than NG, irrespective of the medium with the exception of <it>E. coli </it>grown in LB. Also, enumeration of cells after staining with 4',6-diamidino-2-phenylindole showed that significantly higher numbers were achieved under MRG conditions during stationary phase for <it>E. coli </it>and <it>S. aureus </it>grown in M9 and dilute LB, respectively. In addition, with the exception of smaller <it>S. aureus </it>volume under MRG conditions at exponential phase in dilute LB, biovolume and protein concentrations per cell did not significantly differ between MRG and NG treatments. Both <it>E. coli </it>and <it>S. aureus </it>had higher average membrane potential and integrity under MRG than NG conditions; however, these responses varied with growth medium and growth phase.</p> <p>Conclusions</p> <p>Overall, our data provides novel information about <it>E. coli </it>and <it>S. aureus </it>membrane potential and integrity and suggest that bacteria are physiologically more active and a larger percentage are viable under MRG as compared to NG conditions. In addition, these results demonstrate that bacterial physiological responses to MRG conditions vary with growth medium and growth phase demonstrating that nutrient resources are a modulator of response.</p
Brief Note: Characterization of Hydrophobic Stream Bacteria Based on Adhesion to n-Octane
Author Institution: Department of Biological Sciences, Kent State UniversityThe purpose of this study was to characterize stream bacterial communities based on cell surface hydrophobicity. Because hydrophobicity is related to adhesion we hypothesized that more hydrophobic bacteria would be found on solid surfaces than in water. Water, rock, and sediment from two northeastern Ohio streams were sampled and bacteria were plated on modified nutrient agar. Hydrophobicity was determined by measuring adherence to n-octane. No difference was found in the proportion of hydrophobic bacteria among habitats. Two hydrophobic isolates were identified as Sphingomonas paucimobilis and Chryseomonas luteola. A large proportion of hydrophobic bacteria were gram positive and urease positive; none were gelatinase positive. More hydrophobic than hydrophilic bacteria were able to grow using manatose or malatose as the only carbon source. These physiological differences indicate that hydrophobic bacteria may be able to utilize resources not available to hydrophilic bacteria
Plataforma interactiva de investigación para las Ciencias Sociales
La Plataforma Interactiva de Investigación para las Ciencias Sociales (PLIICS) es un proyecto del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) cuyo objetivo es fomentar el movimiento de acceso abierto a los repositorios del Sistema Científico mediante la construcción de una plataforma de repositorios digitales de datos de las Ciencias Sociales. Este objetivo se encuentra alineado a las políticas del Ministerio de Ciencia, Tecnología e Innovación Productiva (MINCyT) en materia de acceso abierto a archivos digitales de contenido científico. Esta plataforma incorpora las modalidades usuales en las comunidades científicas y organizaciones referentes de Ciencias Sociales a nivel mundial, como estándares abiertos y software Open Source.
El grupo prueba piloto PLIICS está conformado por especialistas de cinco Institutos de Ciencias Sociales del CONICET y dos coordinadores de Sede Central, para impulsar la construcción de esta Plataforma, y conformar una Comunidad de Práctica que facilite su desarrollo a mediano plazo. La sustentabilidad del proyecto se basa en el interés de los investigadores en disponer de más información científica primaria, y recibir además reconocimiento por los datos publicados (mediante un sistema de citación de datos).
Superadas las primeras experiencias exploratorias, en este momento los Institutos participantes se encuentran desarrollando planes de trabajo para preparar y exponer públicamente sus colecciones de datos primarios en la PLIICS.Sociedad Argentina de Informática e Investigación Operativ
Plataforma interactiva de investigación para las Ciencias Sociales
La Plataforma Interactiva de Investigación para las Ciencias Sociales (PLIICS) es un proyecto del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) cuyo objetivo es fomentar el movimiento de acceso abierto a los repositorios del Sistema Científico mediante la construcción de una plataforma de repositorios digitales de datos de las Ciencias Sociales. Este objetivo se encuentra alineado a las políticas del Ministerio de Ciencia, Tecnología e Innovación Productiva (MINCyT) en materia de acceso abierto a archivos digitales de contenido científico. Esta plataforma incorpora las modalidades usuales en las comunidades científicas y organizaciones referentes de Ciencias Sociales a nivel mundial, como estándares abiertos y software Open Source.
El grupo prueba piloto PLIICS está conformado por especialistas de cinco Institutos de Ciencias Sociales del CONICET y dos coordinadores de Sede Central, para impulsar la construcción de esta Plataforma, y conformar una Comunidad de Práctica que facilite su desarrollo a mediano plazo. La sustentabilidad del proyecto se basa en el interés de los investigadores en disponer de más información científica primaria, y recibir además reconocimiento por los datos publicados (mediante un sistema de citación de datos).
Superadas las primeras experiencias exploratorias, en este momento los Institutos participantes se encuentran desarrollando planes de trabajo para preparar y exponer públicamente sus colecciones de datos primarios en la PLIICS.Sociedad Argentina de Informática e Investigación Operativ
Plataforma interactiva de investigación para las Ciencias Sociales
La Plataforma Interactiva de Investigación para las Ciencias Sociales (PLIICS) es un proyecto del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) cuyo objetivo es fomentar el movimiento de acceso abierto a los repositorios del Sistema Científico mediante la construcción de una plataforma de repositorios digitales de datos de las Ciencias Sociales. Este objetivo se encuentra alineado a las políticas del Ministerio de Ciencia, Tecnología e Innovación Productiva (MINCyT) en materia de acceso abierto a archivos digitales de contenido científico. Esta plataforma incorpora las modalidades usuales en las comunidades científicas y organizaciones referentes de Ciencias Sociales a nivel mundial, como estándares abiertos y software Open Source.
El grupo prueba piloto PLIICS está conformado por especialistas de cinco Institutos de Ciencias Sociales del CONICET y dos coordinadores de Sede Central, para impulsar la construcción de esta Plataforma, y conformar una Comunidad de Práctica que facilite su desarrollo a mediano plazo. La sustentabilidad del proyecto se basa en el interés de los investigadores en disponer de más información científica primaria, y recibir además reconocimiento por los datos publicados (mediante un sistema de citación de datos).
Superadas las primeras experiencias exploratorias, en este momento los Institutos participantes se encuentran desarrollando planes de trabajo para preparar y exponer públicamente sus colecciones de datos primarios en la PLIICS.Sociedad Argentina de Informática e Investigación Operativ
Initial nitrogen enrichment conditions determines variations in nitrogen substrate utilization by heterotrophic bacterial isolates
Background The nitrogen (N) cycle consists of complex microbe-mediated transformations driven by a variety of factors, including diversity and concentrations of N compounds. In this study, we examined taxonomic diversity and N substrate utilization by heterotrophic bacteria isolated from streams under complex and simple N-enrichment conditions. Results Diversity estimates differed among isolates from the enrichments, but no significant composition were detected. Substrate utilization and substrate range of bacterial assemblages differed within and among enrichments types, and not simply between simple and complex N-enrichments. Conclusions N substrate use patterns differed between isolates from some complex and simple N-enrichments while others were unexpectedly similar. Taxonomic composition of isolates did not differ among enrichments and was unrelated to N use suggesting strong functional redundancy. Ultimately, our results imply that the available N pool influences physiology and selects for bacteria with various abilities that are unrelated to their taxonomic affiliation. Electronic supplementary material The online version of this article (doi:10.1186/s12866-017-0993-7) contains supplementary material, which is available to authorized users
The effectiveness of case management for comorbid diabetes type 2 patients; the CasCo study. Design of a randomized controlled trial
BACKGROUND: More than half of the patients with type 2 diabetes (T2DM) patients are diagnosed with one or more comorbid disorders. They can participate in several single-disease oriented disease management programs, which may lead to fragmented care because these programs are not well prepared for coordinating care between programs. Comorbid patients are therefore at risk for suboptimal treatment, unsafe care, inefficient use of health care services and unnecessary costs. Case management is a possible model to counteract fragmented care for comorbid patients. It includes evidence-based optimal care, but is tailored to the individual patients' preferences.The objective of this study is to examine the effectiveness of a case management program, in addition to a diabetes management program, on the quality of care for comorbid T2DM patients. METHODS/DESIGN: The study is a randomized controlled trial among patients with T2DM and at least one comorbid chronic disease (N=230), who already participate in a diabetes management program. Randomization will take place at the level of the patients in general practices. Trained practice nurses (case managers) will apply a case management program in addition to the diabetes management program. The case management intervention is based on the Guided Care model and includes six elements; assessing health care needs, planning care, create access to other care providers and community resources, monitoring, coordinating care and recording of all relevant information. Patients in the control group will continue their participation in the diabetes management program and receive care-as-usual from their general practitioner and other care providers. DISCUSSION: We expect that the case management program, which includes better structured care based on scientific evidence and adjusted to the patients' needs and priorities, will improve the quality of care coordination from both the patients' and caregivers' perspective and will result in less consumption of health care services. TRIAL REGISTRATION: Netherlands Trial Register (NTR): NTR1847. (aut. ref.
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