Intervención comunicacional en un emprendimiento digital : el caso de Cuchillos Argentinos

El presente trabajo consiste en la intervención comunicacional de un emprendimiento rosarino Cuchillos Argentinos, en el marco de las plataformas virtuales. El período trabajado es desde octubre de 2019 a octubre de 2020. Se plantea describir la lógica de comunicación actual, con el objetivo general de proponerle acciones que le brinden un crecimiento en cuanto a su alcance. Para el abordaje de la investigación las técnicas metodológicas utilizadas fueron, entrevista semiestructurada y observación etnográfica virtual. Las mismas generaron inquietudes que posibilitaron delinear diferentes hallazgos. Estas herramientas fueron claves para proponer acciones que se traduzcan en aportes concretos hacia el emprendimiento. La Tesina concluye con la importancia de generar un plan comunicacional como eje de cualquier tipo de intervención disciplinar. Definido en tanto a su estructura y desarrollo, en concordancia con el público objetivo de la organización.Fil: Trujillo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Trabajo Social; Argentina

Markers of inflammation and cardiovascular disease in recently diagnosed celiac disease patients

AIM: To evaluate novel risk factors and biomarkers of cardiovascular disease in celiac disease (CD) patients compared with healthy controls.METHODS:Twenty adult patients with recent diagnosis of CD and 20 sex, age and body mass index-matched healthy controls were recruited during a period of 12 mo.Indicators of carbohydrate metabolism, hematological parameters and high sensitive C reactive protein were determined. Moreover, lipoprotein metabolism was also explored through evaluation of the lipid profile and the activity of cholesteryl ester transfer protein and lipoprotein associated phospholipase A2, which is also considered a specific marker of vascular inflammation. The protocol was approved by the Ethic Committee from School of Pharmacy and Biochemistry, University of Buenos Aires and from Buenos Aires Italian Hospital, Buenos Aires, Argentina.RESULTS: Regarding the indicators of insulin resistance, CD patients showed higher plasma insulin levels [7.2 (5.0-11.3) mU/L vs 4.6 (2.6-6.7) mU/L, P < 0.05], increased Homeostasis Model Assessment-Insulin Resistance [1.45 (1.04-2.24) vs 1.00 (0.51-1.45), P < 0.05] and lower Quantitative Sensitive Check index [0.33 (0.28-0.40) vs 0.42 (0.34-0.65), P < 0.05] indexes. Folic acid concentration [5.4 (4.4-7.9) ng/mL vs 12.2 (8.0-14.2) ng/mL, P < 0.01] resulted to be lower and High-sensitivity C reactive protein levels higher (4.21 ± 6.47 mg/L vs 0.98 ± 1.13 mg/L, P < 0.01) in the patient group. With respect to the lipoprotein profile, CD patients showed lower high density lipoproteincholesterol (HDL-C) (45 ± 15 mg/dL vs 57 ± 17 mg/dL, P < 0.05) and apo A-I (130 ± 31 mg/dL vs 155 ± 29 mg/ dL, P < 0.05) levels, as well as higher total cholesterol/ HDL-C [4.19 (3.11-5.00) vs 3.52 (2.84-4.08), P < 0.05] and apo B/apo A-I (0.75 ± 0.25 vs 0.55 ± 0.16, P < 0.05) ratios in comparison with control subjects. No statistically significant differences were detectedin lipoprotein-associated lipid transfer protein and enzymes.CONCLUSION: The presence and interaction of the detected alterations in patients with CD, would constitute a risk factor for the development of atherosclerotic cardiovascular disease.Fil: Tetzlaff, Walter Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Meroño, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Menafra, Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Martin, Maximiliano. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Botta, Eliana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Matoso, María Dolores. Hospital Italiano; ArgentinaFil: Sorroche, Patricia Beatriz. Hospital Italiano; ArgentinaFil: De Paula, Juan A. Hospital Italiano; ArgentinaFil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Brites, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentin

High risk of cardiovascular disease in iron overload patients

Introduction: Iron overload (IO) is defined as an increase in storage iron, regardless of the presence or absence of tissue damage. Whether increased iron stores are involved in the pathogenesis of cardiovascular disease remains controversial.Objectives: To study insulin resistance markers, lipoprotein profile, activities of anti and prooxidant enzymes and cholesteryl ester transfer protein (CETP) in patients with IO.Methods: Twenty patients with IO were compared with 20 sex and age-matched controls. General biochemical parameters, lipoprotein profile, and activities of paraoxonase 1, employing two substrates, paraoxon (PON) and phenylacetate (ARE), lipoprotein-associated phospholipase A2 (Lp-PLA2) and CETP were determined.Results: IO patients showed higher levels of HOMA-IR and triglycerides (median [Q1-Q3]) (128[93-193] vs. 79[51-91]mg/dl,p<0.0005) while lower HDL-cholesterol (mean±SD) (41±9 vs. 52±10mg/dl,p<0.0005) in comparison with controls. Moreover, the triglycerides/HDL-cholesterol (3.2[2.0-5.1] vs. 1.5[1.0-1.9],p<0.0005) ratio and oxidized LDL levels (94[64-103] vs. 68[59-70]IU/l,p<0.05) were increased in the patient group. Though no difference was observed in ARE activity, PON activity was decreased in IO patients (246[127-410] vs. 428[263-516]nmol.ml-1.min-1,p<0.05). In addition, CETP and Lp-PLA2 activities were also increased in the patients (189±31 vs. 155±36%.ml-1.h-1,p<0.005; and 10.1±2.9 vs. 8.2±2.4µmol.ml-1.h-1,p<0.05, respectively). Associations between ferritin concentration and the alterations in lipid metabolism were also found. Multiple regression analyses identified HOMA-IR as independent predictor of CETP activity (B=65.9,p<0.0001,r2=0.35), as well as ferritin concentration of Lp-PLA2 activity (B=3.7,p<0.0001,r2=0.40) after adjusting for confounding variables.Conclusions: IO patients presented not only insulin resistance, but also metabolic alterations which were related to elevated iron stores and are associated to high risk of cardiovascular disease.Fil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Sorroche, Patricia. Hospital Italiano; ArgentinaFil: Boero, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Arbelbide, Jorge. Hospital Italiano; ArgentinaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin

Alterations in biomarkers of cardiovascular disease (CVD) in active acromegaly

Objectives: In acromegalic patients, cardiovascular and metabolic comorbidities contribute to enhance mortality. Available data on the lipoprotein profile of these patients are controversial. Our aim was to characterize the lipoprotein profile and emergent biomarkers of cardiovascular disease in active acromegalic patients in comparison with sex- and age-matched healthy controls. Patients: Eighteen patients with active acromegaly and 18 controls were studied. Measurements: Glucose levels, hormonal status, lipoprotein profile and C reactive protein (CRP) were evaluated by standardized methods. Cholesteryl ester transfer protein (CETP) and lipoprotein-associated phospholipase A2 (Lp-PLA2) were measured by radiometric techniques, endothelin-1 and vascular cell adhesion molecule (VCAM)-1 by enzyme-linked immunosorbent assay, and leucocytes CD18, CD49d and CD54 by flow cytometry. Results: After adjusting for body mass index (BMI), acromegalic patients presented a more atherogenic lipoprotein profile, consisting of higher levels of triglycerides and apolipoprotein B and alterations in the ratios which estimate insulin resistance and atherogenic risk. CETP activity was significantly increased in acromegalic patients as compared to controls (168 ± 17 vs. 141 ± 30% per ml h, respectively; P < 0.05). Endothelin-1 levels evidenced an increase in the patients' group (0.9 ± 0.2 vs. 0.7 ± 0.2 ng/l, respectively; P < 0.01) and showed positive and significant correlations with GH, IGF-1 and IGFBP-3 (r = 0.45, 0.42 and 0.44, respectively; P < 0.01 for all of them; with BMI as a fixed variable). Lymphocytes from acromegalic patients showed increased CD49d content (282 ± 59 vs. 246 ± 48 arbitrary units, respectively; P < 0.05). Conclusions: Taken together, the alterations described seem to contribute to constituting a state of higher propensity for the development of atherosclerotic cardiovascular disease, which adds to the presence of specific cardiomyopathy.Fil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Manavela, M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Insua, C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Berardi, V.. No especifíca;Fil: Fornari, M.C.. No especifíca;Fil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin

Deterministic Factors Overwhelm Stochastic Environmental Fluctuations as Drivers of Jellyfish Outbreaks

16 pages, 4 figures, 1 table, supporting Information http://dx.doi.org/10.1371/journal.pone.0141060Jellyfish outbreaks are increasingly viewed as a deterministic response to escalating levels of environmental degradation and climate extremes. However, a comprehensive understanding of the influence of deterministic drivers and stochastic environmental variations favouring population renewal processes has remained elusive. This study quantifies the deterministic and stochastic components of environmental change that lead to outbreaks of the jellyfish Pelagia noctiluca in the Mediterranen Sea. Using data of jellyfish abundance collected at 241 sites along the Catalan coast from 2007 to 2010 we: (1) tested hypotheses about the influence of time-varying and spatial predictors of jellyfish outbreaks; (2) evaluated the relative importance of stochastic vs. deterministic forcing of outbreaks through the environmental bootstrap method; and (3) quantified return times of extreme events. Outbreaks were common in May and June and less likely in other summer months, which resulted in a negative relationship between outbreaks and SST. Cross- and along-shore advection by geostrophic flow were important concentrating forces of jellyfish, but most outbreaks occurred in the proximity of two canyons in the northern part of the study area. This result supported the recent hypothesis that canyons can funnel P. noctiluca blooms towards shore during upwelling. This can be a general, yet unappreciated mechanism leading to outbreaks of holoplanktonic jellyfish species. The environmental bootstrap indicated that stochastic environmental fluctuations have negligible effects on return times of outbreaks. Our analysis emphasized the importance of deterministic processes leading to jellyfish outbreaks compared to the stochastic component of environmental variation. A better understanding of how environmental drivers affect demographic and population processes in jellyfish species will increase the ability to anticipate jellyfish outbreaks in the futureThe authors gratefully acknowledge financial support by the European Community Seventh Framework Programme (FP7/2007–2013) for the project VECTORS (grant agreement no. 266445) (URL: http://cordis.europa.eu/fp7/home_en.html). AC was supported by a doctoral fellowship from the Chilean National Commission for Scientific and Technological Research (CONICYT – PFCHA/Doctorado al Extranjero 4a Convocatoria, 72120016).Peer Reviewe

Low plasma triiodothyronine levels in heart failure are associated with a reduced anabolic state and membrane damage

BACKGROUND: Low plasma triiodothyronine (T(3)) levels are considered a prognostic predictor of death in heart failure (HF) patients. AIM: To study an association between plasma T(3) levels and several cardiac, neurohormonal, and metabolic markers of HF. METHODS: A total of 133 ambulatory HF patients (114 males; mean age 63.2 years) with left ventricular ejection fraction <40% were enrolled. TSH, total tetraiodothyronine (T(4)) and T(3), N-terminal pro-brain natriuretic peptide (NT-proBNP), and other cardiac and metabolic parameters were measured. The lowest tertile of T(3) (group 1) was compared against the two upper ones (group 2). RESULTS: In simple logistic regression, the lowest T(3) tertile was associated with more advanced HF disease status: older (age: odds ratio (OR)=1.05; confidence interval (CI) 95% 1.01-1.09, P=0.004), lower functional capacity (walking test: OR=0.996; CI 95% 0.993-0.999, P=0.008), higher NT-proBNP (OR=1.64; CI 95% 1.19-2.27, P=0.003) and adiponectin levels (OR=1.07; CI 95% 1.02-1.11, P=0.004), lower DHEAS log-transformed (OR=0.50; CI 95% 0.31-0.80, P=0.004), and the presence of lower phase angle values as measured by body bioelectrical impedance analysis (OR=3.18; CI 95% 1.50-6.71, P=0.04) and worse renal function (OR=0.96; CI 95% 0.94-0.98, P=0.003). T(3) levels in the lowest tertile were independently associated with low phase angle values (OR=2.95, CI 95% 1.16-7.50, P=0.02) and the log transformation of DHEAS (OR=0.56; CI 95% 0.32-0.97, P=0.04). CONCLUSION: We have demonstrated an association between plasma T(3) levels in the lower range and other deranged hormonal and metabolic parameters in HF patients.Fil: Brenta, Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital César Milstein; ArgentinaFil: Thierer, Jorge. Instituto Cardiovascular de Buenos Aires; ArgentinaFil: Sutton, Marcela. Instituto Cardiovascular de Buenos Aires; ArgentinaFil: Acosta, Adriana. Instituto Cardiovascular de Buenos Aires; ArgentinaFil: Vainstein, Nora. Instituto Cardiovascular de Buenos Aires; ArgentinaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Boero, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Anker, Stefan. Istituto di Ricovero e Cura a Carattere Scientifico; Italia. Charité Universitätsmedizin Berlin; Alemani

Targeting of multiple metabolites in neural cells monitored by using protein-based carbon nanotubes

Microdevices dedicated to monitor metabolite levels have recently enabled many applications in the field of cell analysis, to monitor cell growth and development of numerous cell lines. By combining the traditional technology used for electrochemical biosensors with nanoscale materials, it is possible to develop miniaturized metabolite biosensors with unique properties of sensitivity and detection limit. In particular, enzymes tend to adsorb onto carbon nanotubes and their optical or electrical activity can perturb the electronic properties. In the present work we propose multi-walled carbon nanotube-based biosensors to monitor a cell line highly sensitive to metabolic alterations, in order to evaluate lactate production and glucose uptake during different cell states. We achieve sensors for both lactate and glucose, with sensitivities of 40.1 mu A mM(-1) cm(-2) and 27.7 mu A mM(-1) cm(-2), and detection limits of 28 mu M and 73 mu M, respectively. This nano-biosensing technology is used to provide new information on cell line metabolism during proliferation and differentiation, which are unprecedented in cell biology. (C) 2011 Elsevier B.V. All rights reserved

Innovación e improvisación en el marco de la pandemia de COVID-19: relato de una experiencia

En el presente trabajo se relata la experiencia de nuestro equipo docente en el marco de la contingencia provocada por la pandemia de COVID-19. Nuestra universidad decidió en este particular contexto ratificar su calendario académico y garantizar la continuidad pedagógica a través de la modalidad de enseñanza a distancia. Este requerimiento nos puso a los docentes de la casa a pensar y a actuar con gran celeridad, seleccionando las herramientas y metodologías que consideramos más adecuadas para garantizar el derecho a continuar estudiando a la mayoría de nuestros estudiantes. En este trabajo, queremos presentar las estrategias didácticas llevadas a cabo por nuestro equipo docente - el cual está a cargo de una comisión de la cátedra Matemática A, de la Facultad de Ingeniería de la Universidad Nacional de La Plata-, algunas de las dificultades que nos hemos encontrado en este camino y algunos resultados que nos permiten valorar positivamente la experiencia.Secretaría de Asuntos Académico

Innovation and improvisation in the framework of the COVID-19 pandemic: an experience report

En el presente trabajo se relata la experiencia de nuestro equipo docente en el marco de la contingencia provocada por la pandemia de COVID-19. Nuestra universidad decidió en este particular contexto ratificar su calendario académico y garantizar la continuidad pedagógica a través de la modalidad de enseñanza a distancia. Este requerimiento nos puso a los docentes de la casa a pensar y a actuar con gran celeridad, seleccionando las herramientas y metodologías que consideramos más adecuadas para garantizar el derecho a continuar estudiando a la mayoría de nuestros estudiantes. En este trabajo, queremos presentar las estrategias didácticas llevadas a cabo por nuestro equipo docente -el cual está a cargo de una comisión de la cátedra Matemática A, de la Facultad de Ingeniería de la Universidad Nacional de La Plata-, algunas de las dificultades que nos hemos encontrado en este camino y algunos resultados que nos permiten valorar positivamente la experiencia.In this paper, the experience of our teaching team in the context of the contingency caused by the COVID-19 pandemic is reported. Our university decided in this particular context to ratify its academic calendar and guarantee pedagogical continuity through the distance learning modality. This requirement put the teachers of the house to think and act with great speed, selecting the tools and methodologies that we consider most appropriate to guarantee the right to continue studying the majority of our students. In this work, we want to present the didactic strategies carried out by our teaching team - which is in charge of a committee of the Mathematical Chair A, of the Faculty of Engineering of the National University of La Plata -, some of the difficulties that We have found ourselves on this path and some results that allow us to positively value the experience.Especialización en Docencia Universitari

Aumento del riesgo de enfermedad cardiovascular en pacientes con enfermedad celíaca

En pacientes con enfermedad celíaca (EC), se ha observado mayor incidencia de eventos cardiovasculares que en controles, sin la presencia de factores de riesgo aterogénico clásicos. El objetivo de este estudio fue evaluar los factores de riesgo nóveles y biomarcadores de inflamación y enfermedad cardiovascular en pacientes con EC, con presentación típica y atípica. Fueron seleccionados 14 pacientes con EC sin tratamiento y controles pareados por sexo y edad. Se determinaron parámetros hematológicos, indicadores del metabolismo de los hidratos de carbono, proteína C reactiva ultrasensible (PCRus), per- fil lipoproteico y actividades de proteína transportadora de colesterol esterificado (CETP) y fosfolipasa A2 asociada a lipoproteínas (Lp-PLA2). Los pacientes con EC presentaron niveles plasmáticos mayores de insulina (7,2 mU/l vs. 4,4 mU/l; p<0,05) y mayor índice HOMA-IR (1,45 vs. 0,98; p<0,05) que los controles. Por otro lado, se observó menor concentración de colesterol-HDL (50 vs. 62 mg/dl; p<0,05), mayor cociente triglicéridos/colesterol-HDL y niveles de PCRus más altos (4,56 vs. 1,17 mg/l; p<0,05) en los pacientes que en los controles. Al comparar a los pacientes con presentación típica (n=8) y atí- pica (n=6), aquellos con presentación típica mostraron menores niveles de apo A-I (128 vs. 178 mg/dl; p<0,01) y aumento del cociente apo B/apo A-I (0,72 vs. 0,43; p<0,05), así como mayor actividad de LpPLA2 (7,9 umol/ml.h vs. 6,15 umol/ml.h; p<0,05). La interacción de las alteraciones descriptas durante períodos de tiempo prolongados en una condición patológica crónica como la EC constituirían un mayor riesgo de desarrollo de enfermedad cardiovascular aterosclerótica.In patients with celiac disease (CD), it has been reported higher incidence of cardiovascular events than in controls, without the presence of classical atherogenic risk factors. The aim of this study was to evaluate the novel risk factors and biomarkers of inflammation and cardiovascular disease in patients with CD, with typical and atypical presentation. We selected 14 patients with CD without treatment and 14 healthy sex and age-matched controls. Haematological parameters, indicators of carbohydrates metabolism, high sensitive C reactive protein (hsCRP), lipoprotein profile and the activities of cholesteryl ester transfer protein (CETP) and lipoprotein-associated phospholipase A2 (Lp-PLA2) were determined. CD patients presented higher insulin plasma levels (7.2 mU/l vs. 4.4 mU/l, p <0.05) and increased HOMA-IR index (1.45 vs. 0.98, p <0.05) than controls. On the other hand, lower HDLcholesterol concentration (50 vs. 62 mg/dl, p<0.05), higher TG/HDL-cholesterol ratio and increased hsCRP levels (4.56 vs. 1.17 mg / l, P <0.05) were observed in comparison with control subjects. When comparing patients with typical (n=8) and atypical (n=6) presentation, the former showed lower apo A-I levels (128 vs. 178 mg/dl, p<0.01), and higher apo B/apo A-I ratio (0.72 vs. 0.43, p<0.05) and LpPLA2 activity (7.9 umol/ml.h vs. 6.15 umol/ml.h, p<0.05). The interaction among the alterations above described during long periods of time in a chronic pathological condition such as CD could constitute higher risk of development of atherosclerotic cardiovascular disease.Fil: Menafra, Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matoso, María Dolores. Hospital Italiano; ArgentinaFil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saez, María Soledad. Hospital Italiano; ArgentinaFil: Sorroche, Patricia Beatriz. Hospital Italiano; ArgentinaFil: de Paula, P.. Hospital Italiano; ArgentinaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin