Assessing the effects of a sequestered germline on interdomain lateral gene transfer in Metazoa

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137230/1/evo12935_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137230/2/evo12935-sup-0001-figure1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137230/3/evo12935-sup-0002-Tables.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137230/4/evo12935.pd

OG# to Protein List

An excel file listing the protein name, abbreviation, found KEGG function (if known), and the corresponding OG5 number of the alignment for all 58 presence/absence lateral gene transfers found. Each animo acid sequence alignment file is labeled with a unique OG5#

Topologies with two LGT Events

Single gene trees in Newick format for the seven genes found with topologies consistent with two lateral gene transfers

Presence/absence alignments

All 58 laterally transferred presence/absence animo acid sequence alignments found. Files are in text format

Laboratory-scale evaluation of microcystin persistence following treatment of five USEPA-registered algaecides at different temperatures

An increasingly warmer climate and unsustainable land-use practices have exacerbated harmful algal blooms in many water resources. Algaecides are a proven treatment to mitigate blooms, but different species and limnological variables can impact efficacy. Water temperature is a variable that affects algaecide efficacy and toxin persistence. In this experiment, microcystin-producing Planktothrix was collected from Grand Lake St. Mary’s in March 2021. Samples were exposed to five different copper and peroxide-based USEPA-registered algaecides at varying concentrations within label rate and incubated at 10°C, 15°C, and 20°C. Total and extracellular microcystins were measured 96 hours after treatment. At 10°C and lowest dosage, PAK 27® reduced total microcystins by 75% compared to the control, followed by GreenClean®Liquid (66%), Cutrine® (63%), SeClear® (55%), and Aligimycin® (40%). In most trials, the amount of total microcystins reduced decreased as temperature increased. However, GreenClean® resulted in a shift to nearly 100% extracellular microcystin at all temperatures, while PAK 27® resulted in 20% extracellular microcystins at 10° and less at higher temperatures. The other three algaecides also shifted microcystins to extracellular. In general, temperature had a greater impact on total microcystin reduction than dosage. This study provides important baseline data for the development of temperature-specific management decisions

Statistical parsimony network showing relationships among 21 mtDNA-IGS haplotypes of the genus <i>Fucus</i> (Table 1) in North America.

<p>The square box represents the haplotype that the program TCS hypothesized as the ancestral haplotype. Solid circles indicate mutational steps, solid line also indicates a mutational step.</p

Sampling location, information, and GenBank number for <i>Fucus</i> collected during the study.

<p>Sampling location, information, and GenBank number for <i>Fucus</i> collected during the study.</p

A dense patch of a small (dwarf) form of <i>Fucus distichus</i> in a lower intertidal crevice on a shore fully exposed to the Labrador Sea.

<p>This shore is scoured by dense, and sometimes moving sea ice from December through April of most years. Between storms and open water intervals, this shore is encased in an extensive ice foot. Battle Harbour, Labrador. Photo: W. Adey.</p

Maximum Likelihood Tree based on mtDNA-IGS for <i>Fucus distichus</i>, <i>F</i>. <i>evanescens</i>, <i>F</i>. <i>garderni</i>, and <i>F serratus</i> specimens from this study and from GenBank (Table 1).

<p>This tree includes neighbor-joining, maximum parsimony, and maximum likelihood bootstrap replicates (1000 replicates); over 50% are indicated at the nodes. The sequence of <i>F</i>. <i>vesiculosus</i> (NC_007683) is used as an outgroup.</p