Immuno-analysis of microparticles: Probing at the limits of detection

Microparticle (MP) research is clouded by debate regarding the accuracy and validity of flow cytometry (FCM) as an analytical methodology, as it is influenced by many variables including the pre-analytical conditions, instruments physical capabilities and detection parameters. This study utilises a simplistic in vitro system for generating MP, and through comparative analysis with immuno-electron microscopy (Immuno-EM) assesses the strengths and limitations of probe selection and high-sensitivity FCM. Of the markers examined, MP were most specifically labelled with phosphatidylserine ligands, annexin V and lactadherin, although only ∼60% MP are PS positive. Whilst these two ligands detect comparable absolute MP numbers, they interact with the same population in distinct manners; annexin V binding is enhanced on TNF induced MP. CD105 and CD54 expression were, as expected, consistent and enhanced following TNF activation respectively. Their labelling however accounted for as few as 30-40% of MP. The greatest discrepancies between FCM and I-EM were observed in the population solely labelled for the surface antigen. These findings demonstrate that despite significant improvements in resolution, high-sensitivity FCM remains limited in detecting small-size MP expressing low antigen levels. This study highlights factors to consider when selecting endothelial MP probes, as well as interpreting and representing data

Improved spatial separation of neutral molecules

We have developed and experimentally demonstrated an improved electrostatic deflector for the spatial separation of molecules according to their dipole-moment-to-mass ratio. The device features a very open structure that allows for significantly stronger electric fields as well as for stronger deflection without molecules crashing into the device itself. We have demonstrated its performance using the prototypical OCS molecule and we discuss opportunities regarding improved quantum-state-selectivity for complex molecules and the deflection of unpolar molecules.Comment: 6 figure

A Photometric Survey for Variables and Transits in the Field of Praesepe with KELT

The Kilodegree Extremely Little Telescope (KELT) project is a small aperture, wide-angle search for planetary transits of solar-type stars. In this paper, we present the results of a commissioning campaign with the KELT telescope to observe the open cluster Praesepe for 34 nights in early 2005. Lightcurves were obtained for 69,337 stars, out of which we identify 58 long period variables and 152 periodic variables. Sixteen of these are previously known as variable, yielding 194 newly discovered variable stars for which we provide properties and lightcurves. We also searched for planetary-like transits, finding four transit candidates. Follow-up observations indicate that two of the candidates are astrophysical false positives, with two candidates remaining as potential planetary transits.Comment: 45 pages, 16 figures. Submitted to AJ. PDF version with full resolution figures located at http://www.astronomy.ohio-state.edu/~pepper/kelt.pd

A Case Study on the Advantages of 3D Walkthroughs over Photo Stitching Techniques

Virtual tours and interactive walkthroughs enable a more in-depth platform for communicating information. Many current techniques employ the use of Photo Stitching to accomplish this. However, over the last decade advancements in computing power and the accessibility of game engines, meant that developing rich 3D content for virtual tours is more possible than ever before. As such, the purpose of this paper is to present a study into the advantages of developing an interactive 3D virtual tour of student facilities, using the Unreal Development 4 Game Engine, for educational establishments. The project aims to demonstrate a comparison between the use of Photo Stitching and 3D Modelled interactive walkthrough for developing rich visual environments. The research reveals that the approach in this paper can improve educational facilities prominence within universities, and contains many advantages over Photo Stitching techniques

On Predicting Learning Styles in Conversational Intelligent Tutoring Systems using Fuzzy Decision Trees

Intelligent Tutoring Systems personalise learning for students with different backgrounds, abilities, behaviours and knowledge. One way to personalise learning is through consideration of individual differences in preferred learning style. OSCAR is the name of a Conversational Intelligent Tutoring System that models a person's learning style using natural language dialogue during tutoring in order to dynamically predict, and personalise, their tutoring session. Prediction of learning style is undertaken by capturing independent behaviour variables during the tutoring conversation with the highest value variable determining the student's learning style. A weakness of this approach is that it does not take into consideration the interactions between behaviour variables and, due to the uncertainty inherently present in modelling learning styles, small differences in behaviour can lead to incorrect predictions. Consequently, the learner is presented with tutoring material not suited to their learning style. This paper proposes a new method that uses fuzzy decision trees to build a series of fuzzy predictive models combining these variables for all dimensions of the Felder Silverman Learning Styles model. Results using live data show the fuzzy models have increased the predictive accuracy of OSCAR-CITS across four learning style dimensions and facilitated the discovery of some interesting relationships amongst behaviour variables

Key dating features for timber-framed dwellings in Surrey

This article is made available through the Brunel Open Access Publishing Fund. Copyright @ The Vernacular Architecture Group 2013. MORE OpenChoice articles are open access and distributed under the terms of the Creative Commons Attribution License 3.0.The main component of the Surrey Dendrochronology Project is the accurate dating of 177 ‘dwellings’, nearly all by tree-ring analysis. The dates are used to establish date ranges for 52 ‘key features’, which cover many aspects of timber-framing from building type to details of carpentry. It is shown that changes of method and fashion were in many cases surprisingly rapid, almost abrupt in historical terms. Previous dating criteria for timber-framed dwellings in the county have been refined and new criteria introduced. Clusters of change from the 1440s and the 1540s are shown and some possible historical links suggested.The Heritage Lottery Fund, the Domestic Buildings Research Group (Surrey), the Surrey Archaeological Society and the historical societies of Charlwood, Farnham and Nutfield

The Viking seismometry

Efforts were made to determine the seismicity of Mars as well as define its internal structure by detecting vibrations generated by marsquakes and meteoroid impacts. The lack of marsquakes recognized in the Viking data made it impossible to make any direct inferences about the interior of Mars and only allowed the setting of upper bounds on the seismic activity of the planet. After obtaining more than 2100 hours worth of data during the quite periods at rates of one sample per second or higher, the Viking 2 seismometer was turned off as a consequence of a landing system failure. During the periods when adequate data were obtained, one event of possible seismic or meteoroid impact origin was recognized; however, there is a significant probability that this event was generated by a wind gust

Using Protoplanetary Disks To Weigh The Youngest Stars And Constrain The Earliest Stages Of Stellar Evolution

Mass is the fundamental property that determines the fate of a star. In particular, the masses of young stars are of great relevance to many astrophysical problems, including star and planet formation. We have developed a novel approach that combines spatially resolved sub-millimeter spectral line imaging and optical/near-infrared high resolution spectroscopy to derive the fundamental properties of a young star: mass, temperature, and radius. By applying our technique to a sample of pre-main sequence stars, we are mapping out a dynamically-calibrated Hertzsprung-Russell diagram for the express purpose of evaluating pre-main sequence evolutionary models. Looking forward, ALMA is poised to deliver precise stellar masses in statistically large quantities, enabling a meaningful survey of the fundamental properties of young stars

The Transit Light Curve Project. VII. The Not-So-Bloated Exoplanet HAT-P-1b

We present photometry of the G0 star HAT-P-1 during six transits of its close-in giant planet, and we refine the estimates of the system parameters. Relative to Jupiter's properties, HAT-P-1b is 1.20 +/- 0.05 times larger and its surface gravity is 2.7 +/- 0.2 times weaker. Although it remains the case that HAT-P-1b is among the least dense of the known sample of transiting exoplanets, its properties are in accord with previously published models of strongly irradiated, coreless, solar-composition giant planets. The times of the transits have a typical accuracy of 1 min and do not depart significantly from a constant period.Comment: To appear in AJ [19pg, 3 figures]. New co-author added. Minor revisions to match published versio

A Keck/HIRES Doppler Search for Planets Orbiting Metal-Poor Dwarfs. I. Testing Giant Planet Formation and Migration Scenarios

We describe a high-precision Doppler search for giant planets orbiting a well-defined sample of metal-poor dwarfs in the field. This experiment constitutes a fundamental test of theoretical predictions which will help discriminate between proposed giant planet formation and migration models. We present here details on the survey as well as an overall assessment of the quality of our measurements, making use of the results for the stars that show no significant velocity variation.Comment: 25 pages, 7 figures, accepted for publication in the Astrophysical Journa