Façons de parler d'Europe

Ce chapitre se propose d'analyser les catégories de sens et de jugement que les enquêtés mobilisent dans l'énonciation de leur opinion sur le thème de l'entretien. Les auteurs se focalisent sur leurs façons de tenir un point de vue sur l'Europe politique, et cherchent à repérer dans quelle mesure leur rapport à l'Europe prend appui dans leurs univers d'existence, c'est-à-dire dans une expérience du social tout à la fois inscrite dans un temps historique et un espace culturel donnés, coordonnée à la détention de propriétés individuelles et à l'appartenance à des collectifs plus ou moins objectivés. Il s'agit, au fond, de rapporter les données sociodémographiques (âge, sexe, revenu, niveau de fin d'études) à des éléments moins facilement objectivables et souvent difficilement comparables à l'échelle de l'Europe communautaire (sentiment d'être un citoyen ou un européen légitime, sécurité dans l'emploi, mode de vie). À partir de cette posture scientifique, le papier vise surtout à analyser les ressources et les techniques d'identification sociale auxquelles les enquêtés recourent pour répondre à une sollicitation sur l'Europe qu'aux catégories statistiques ou juridiques qui leur sont exogènes et desquelles ils seraient censés être les représentants ou les porte-parole " naturels ", notre démarche entend interroger les façons de parler d'Europe à partir des " savoirs " localisés, socialement enracinés

Pratiques d'élevage dans les exploitations bovines allaitantes conduites sur pâturage dans les Hauts de la Réunion

Dans les Hauts de la Réunion, l’élevage bovin constitue la principale activité agricole. L’élevage naisseur «professionnel» représente 8828 vaches mères pour 497 exploitations. Ce sont des exploitations de taille modeste (18 vaches mères en moyenne) dont les performances animales sont en-deçà de leurs homologues métropolitaines. L’objectif de l’étude est d’analyser les pratiques des éleveurs naisseurs et d’en faire le lien avec les performances obtenues afin d’identifier des voies d’amélioration potentielles. Pour cela, 10 éleveurs ont fait l’objet d’un suivi de 1999 à 2002, qui était fondé sur l’évaluation des performances des animaux et sur l’enregistrement des pratiques des éleveurs en particularisant l’allotement, la gestion du pâturage et la complémentation alimentaire. Cet article montre que les pratiques sont variées et conditionnées par des objectifs de production différents: stratégies de maximisation des performances ou du chargement, simplification de la conduite.Cattle farming is the main agricultural activity in the Highlands of the Reunion Island. "Professional" suckling farming represents 8828 cows for 497 farms. They are modest sized farms (18 cows on the average) whose animal performances are lower than their metropolitan counterparts. The aim of the study was to analyse the practices of the suckling farmers with the purpose of improving their management. We carried out herd monitoring on 10 farms from 1999 to 2002 within this framework. This monitoring was based on the measurement of animal performances and on the recording of the farmers' practices in terms of livestock and grazing management. This article presents the analysis carried out on 3 categories of practices: the allotment, grazing management and supplementation. There are various practices underlain by different socioeconomic objectives. Thus, strategies can be founded on the maximisation of the animal performances, the stocking rate or simplification of the management

Loss of Endocan tumorigenic properties after alternative splicing of exon 2

<p>Abstract</p> <p>Background</p> <p>Endocan was originally described as a dermatan sulfate proteoglycan found freely circulating in the blood. Endocan expression confers tumorigenic properties to epithelial cell lines or accelerate the growth of already tumorigenic cells. This molecule is the product of a single gene composed of 3 exons. Previous data showed that endocan mRNA is subject to alternative splicing with possible generation of two protein products. In the present study we identified, and functionally characterized, the alternative spliced product of the endocan gene: the exon 2-deleted endocan, called endocanΔ2.</p> <p>Methods</p> <p>Stable, endocanΔ2-overexpressing cell lines were generated to investigate the biological activities of this new alternatively spliced product of endocan gene. Tumorigenesis was studied by inoculating endocan and endocanΔ2 expressing cell lines subcutaneously in SCID mice. Biochemical properties of endocan and endocanΔ2 were studied after production of recombinant proteins in various cell lines of human and murine origin.</p> <p>Results</p> <p>Our results showed that the exon 2 deletion impairs synthesis of the glycan chain, known to be involved in the pro-tumoral effect of endocan. EndocanΔ2 did not promote tumor formation by 293 cells implanted in the skin of severe combined immunodeficient (SCID) mice.</p> <p>Conclusion</p> <p>Our results emphasize the key role of the polypeptide sequence encoded by the exon 2 of endocan gene in tumorigenesis, and suggest that this sequence could be a target for future therapies against cancer.</p

Implication of scavenger receptors in the interactions between diesel exhaust particles and immature or mature dendritic cells

<p>Abstract</p> <p>Background</p> <p>The exposure to pollutants such as diesel exhaust particles (DEP) is associated with an increased incidence of respiratory diseases. However, the mechanisms by which DEP have an effect on human health are not completely understood. In addition to their action on macrophages and airway epithelial cells, DEP also modulate the functions of dendritic cells (DC). These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. In this study, we assessed the implication of SR in the effects of DEP associated or not with TLR agonists on monocyte-derived DC (MDDC). For this, we studied the regulation of CD36, CXCL16, LOX-1, SR-A1 and SR-B1 expression on MDDC treated with DEP associated or not with TLR2, 3 and 4 ligands. Then, the capacity of SR ligands (dextran sulfate and maleylated-ovalbumin) to block the effects of DEP on the function of lipopolysaccharide (LPS)-activated DC has been evaluated.</p> <p>Results</p> <p>Our data demonstrate that TLR2 agonists mainly augmented CXCL16, LOX-1 and SR-B1 expression whereas DEP alone had only a weak effect. Interestingly, DEP modulated the action of TLR2 and TLR4 ligands on the expression of LOX-1 and SR-B1. Pretreatment with the SR ligand maleylated-ovalbumin but not dextran sulfate inhibited the endocytosis of DEP by MDDC. Moreover, this SR ligand blocked the effect by DEP at low dose (1 μg/ml) on MDDC phenotype (a decrease of CD86 and HLA-DR expression) and on the secretion of CXCL10, IL-12 and TNF-α. In contrast, the decrease of IL-12 and CXCL10 secretion and the generation of oxygen metabolite induced by DEP at 10 μg/ml was not affected by SR ligands</p> <p>Conclusion</p> <p>Our results show for the first time that the modulation of DC functions by DEP implicates SR. TLR agonists upregulated SR expression in contrast to DEP. Interfering with the expression and/or the function of SR might be one way to limit the impact of DEP on lung immune response.</p

ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target

<p>Abstract</p> <p>Background</p> <p>ASPM (<it>Abnormal Spindle-like Microcephaly associated</it>) over-expression was recently implicated in the development of malignant gliomas.</p> <p>Results</p> <p>To better characterize the involvement of ASPM in gliomas, we investigated the mRNA expression in 175 samples, including 8 WHO Grade II, 75 WHO Grade III and 92 WHO Grade IV tumors. <it>Aspm </it>expression was strongly correlated with tumor grade and increased at recurrence when compared to the initial lesion, whatever the initial grade of the primary tumor. ASPM expression also increased over serial passages in gliomaspheres <it>in vitro </it>and in mouse xenografts <it>in vivo</it>. Lentivirus-mediated shRNA silencing of ASPM resulted in dramatic proliferation arrest and cell death in two different gliomasphere models.</p> <p>Conclusion</p> <p>These data suggest that ASPM is involved in the malignant progression of gliomas, possibly through expansion of a cancer stem cell compartment, and is an attractive therapeutic target in glioblastoma multiforme.</p

Spin crossover in Fe(triazole)–Pt nanoparticle self-assembly structured at the sub-5 nm scale

A main goal of molecular electronics is to relate the performance of devices to the structure and electronic state of molecules. Among the variety of possibilities that organic, organometallic and coordination chemistries offer to tune the energy levels of molecular components, spin crossover phenomenon is a perfect candidate for elaboration of molecular switches. The reorganization of the electronic state population of the molecules associated to the spin crossover can indeed lead to a significant change in conductivity. However, molecular spin crossover is very sensitive to the environment and can disappear once the molecules are integrated into devices. Here, we show that the association of ultra-small 1.2 nm platinum nanoparticles with FeII triazole-based spin crossover coordination polymers leads to self-assemblies, extremely well organized at the sub-3 nm scale. The quasi-perfect alignment of nanoparticles observed by transmission electron microscopy, in addition to specific signature in infrared spectroscopy, demonstrates the coordination of the long-chain molecules with the nanoparticles. Spin crossover is confirmed in such assemblies by X-ray absorption spectroscopic measurements and shows unambiguous characteristics both in magnetic and charge transport measurements. Coordinating polymers are therefore ideal candidates for the elaboration of robust, well-organized, hybrid self-assemblies with metallic nanoparticles, while maintaining sensitive functional properties, such as spin crossover

Genetic analysis of variation in human meiotic recombination

The number of recombination events per meiosis varies extensively among individuals. This recombination phenotype differs between female and male, and also among individuals of each gender. In this study, we used high-density SNP genotypes of over 2,300 individuals and their offspring in two datasets to characterize recombination landscape and to map the genetic variants that contribute to variation in recombination phenotypes. We found six genetic loci that are associated with recombination phenotypes. Two of these (RNF212 and an inversion on chromosome 17q21.31) were previously reported in the Icelandic population, and this is the first replication in any other population. Of the four newly identified loci (KIAA1462, PDZK1, UGCG, NUB1), results from expression studies provide support for their roles in meiosis. Each of the variants that we identified explains only a small fraction of the individual variation in recombination. Notably, we found different sequence variants associated with female and male recombination phenotypes, suggesting that they are regulated by different genes. Characterization of genetic variants that influence natural variation in meiotic recombination will lead to a better understanding of normal meiotic events as well as of non-disjunction, the primary cause of pregnancy loss. © 2009 Chowdhury et al

WA92: a fixed target experiment to trigger on and identify beauty particle decays

We describe the detectors and trigger system used in the CERN WA92 experiment. The experiment was designed to study the production and decay of beauty particles from 350 GeV/$c\,$ $\,\pi^-$ interactions in copper and tungsten targets. Charged particle tracking is performed using the Omega spectrometer. Silicon microstrip detectors are used to provide precise tracking information in the region of the production and the decay of heavy-flavoured particles and to trigger on the resulting high impact parameter tracks. The precision of vertex reconstruction corresponds to $\pm 3.7\%$ of the mean B-decay proper lifetime. Lepton and high transverse momentum hadron signals are also used in the trigger, which accepts 29\% of B-decays and rejects 98\% of non-beauty interactions

Measurement of the beauty production cross-section in 350 GeV/c π−\pi^- -Cu interactions

Using a sample of $10^8$ triggered events, produced in $\pi^-$--$\,$Cu interactions at 350~GeV$/c$, we have identified 26 beauty events. The estimated background in this sample is $0.6 \pm 0.6$ events. From these data, assuming a linear A-dependence, we measure a beauty production cross-section integrated over all $x_F$ of $5.7 {+1.3 \atop -1.1}~{\mathrm {(stat.)}} {+0.6 \atop -0.5}~{\mathrm {(syst.)}}~$nb/N

Life in an arsenic-containing gold mine: Genome and physiology of the autotrophic arsenite-oxidizing bacterium Rhizobium sp. NT-26:

Arsenic is widespread in the environment and its presence is a result of natural or anthropogenic activities. Microbes have developed different mechanisms to deal with toxic compounds such as arsenic and this is to resist or metabolize the compound. Here, we present the first reference set of genomic, transcriptomic and proteomic data of an Alphaproteobacterium isolated from an arseniccontaining goldmine: Rhizobium sp. NT-26. Although phylogenetically related to the plant-associated bacteria, this organism has lost the major colonizing capabilities needed for symbiosis with legumes. In contrast, the genome of Rhizobium sp. NT-26 comprises a megaplasmid containing the various genes, which enable it to metabolize arsenite. Remarkably, although the genes required for arsenite oxidation and flagellar motility/biofilm formation are carried by the megaplasmid and the chromosome, respectively, a coordinate regulation of these two mechanisms was observed. Taken together, these processes illustrate the impact environmental pressure can have on the evolution of bacterial genomes, improving the fitness of bacterial strains by the acquisition of novel functions. © The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution