14 research outputs found
Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΈΠ»Ρ ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ² HaCaT Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ 1DE-Π³Π΅Π»Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ
Using tandem mass spectrometry with electrospray ionization, a comparative analysis of HaCaT keratinocyte proteins was carried out before and after exposure of cells to sodium dodecyl sulfate (25 mg/ml) for 48 hours; proteins encoded by human chromosome 18 genes were chosen as the comparison proteins. A total of 2418 proteins were detected in the HaCaT immortalized human keratinocytes, 70% of these proteins were identified by two or more unique peptides. Panoramic mass spectrometry analysis identified 38 proteins encoded by chromosome 18 genes, 27 proteins were common to control HaCaT cells and HaCaT cells exposed to SDS. Using the Metascape database (https://metascape.org), an enrichment analysis of GO terms of the Biological Process category of chromosome 18 gene encoded proteins of HaCaT keratinocytes was performed before and after the SDS exposure. The SDS exposure resulted in a slight enrichment of the GO term "response to stimulus" (GO:0050896) and the related GO term "negative regulation of biological process" (GO:0048519). We found decreased expression levels of membrane proteins encoded by chromosome 18 genes related to cell-cell adhesion (GO:0098609), such as DSC1, DSC3, and DSG1. A decrease in the expression level of desmosomal cadherins is characteristic of malignant neoplasms developing from epithelial tissue cells of various internal organs, mucous membranes, and skin. The method of preparation of HaCaT keratinocyte samples used in this work increased the sensitivity of proteomic analysis of cell culture and made it possible to identify twice as many proteins in one gel strip as compared to the number of proteins (1284) in HaCaT samples subjected to osmotic shock and cleavage by trypsin in solution.ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΎΡΠ΅Π½ΠΊΠ° ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ², ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π½Π° ΡΠΎΠ»ΡΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ 0.2% Π΄ΠΎΠ΄Π΅ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ° Π½Π°ΡΡΠΈΡ (SDS), ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ΅ 1DE-Π³Π΅Π»Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ (SDS-PAGE Π±Π΅Π· ΡΡΠ°ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΡΠ°Π·Π΄Π΅Π»ΡΡΡΠ΅ΠΌ Π³Π΅Π»Π΅) ΠΈ ΡΠ°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠΈ ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠΌ Π² Π³Π΅Π»Π΅ Π΄Π»Ρ ΡΠ³Π»ΡΠ±Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ² ΠΠ°Π‘Π°Π’ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΠ΅ Π±Π΅Π»ΠΊΠ°. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ°Π½Π΄Π΅ΠΌΠ½ΠΎΠΉ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ Ρ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΏΡΠ΅ΠΉΠ½ΠΎΠΉ ΠΈΠΎΠ½ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ (LC-MS/MS) ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π±Π΅Π»ΠΊΠΎΠ² ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ² ΠΠ°Π‘Π°Π’ Π΄ΠΎ ΠΈ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ SDS Π² ΡΡΠ±ΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΎΠ·Π΅ (25 ΠΌΠ³/ΠΌΠ») Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 48 Ρ. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π±Π΅Π»ΠΊΠΎΠ² ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Π²ΡΠ±ΡΠ°Π½Ρ Π±Π΅Π»ΠΊΠΈ, ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΠ΅ Π³Π΅Π½Π°ΠΌΠΈ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 18 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΡΠ΅Π³ΠΎ Π² ΠΈΠΌΠΌΠΎΡΡΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠ°Ρ
ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π»ΠΈΠ½ΠΈΠΈ ΠΠ°Π‘Π°Π’ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ 2418 Π±Π΅Π»ΠΊΠΎΠ², ΠΈΠ· Π½ΠΈΡ
ΠΎΠΊΠΎΠ»ΠΎ 70% ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΏΠΎ Π΄Π²ΡΠΌ ΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΠΌ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌ. ΠΠΎ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌ ΠΏΠ°Π½ΠΎΡΠ°ΠΌΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ΄Π°Π»ΠΎΡΡ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ 38 Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΡ
Π³Π΅Π½Π°ΠΌΠΈ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 18; ΠΈΠ· Π½ΠΈΡ
27 Π±Π΅Π»ΠΊΠΎΠ² Π±ΡΠ»ΠΈ ΠΎΠ±ΡΠΈΠΌΠΈ Π΄Π»Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ°Π‘Π°Π’, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π½ΡΡΡΡ
Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ SDS. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°Π·Ρ Π΄Π°Π½Π½ΡΡ
Metascape Π±ΡΠ» ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΌΠΈΠ½Π°ΠΌΠΈ ΠΎΠ½ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π³Π΅Π½ΠΎΠ² (GO) ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΡ (biological process) Π±Π΅Π»ΠΊΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 18 ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ² ΠΠ°Π‘Π°Π’ Π΄ΠΎ ΠΈ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ SDS. ΠΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ SDS ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½ΠΈΡ GO ΡΠ΅ΡΠΌΠΈΠ½Π° βΠΎΡΠ²Π΅Ρ Π½Π° ΡΡΠΈΠΌΡΠ»β (GO:0050896 - response to stimulus) ΠΈ ΡΠ²ΡΠ·Π°Π½Π½ΠΎΠ³ΠΎ Ρ Π½ΠΈΠΌ GO ΡΠ΅ΡΠΌΠΈΠ½Π° βΠ½Π΅Π³Π°ΡΠΈΠ²Π½Π°Ρ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ²β (GO:0048519 - negative regulation of biological process). ΠΡΠ»ΠΎ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΡ
Π³Π΅Π½Π°ΠΌΠΈ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 18, ΠΎΡΠ½ΠΎΡΡΡΠΈΡ
ΡΡ ΠΊ ΠΌΠ΅ΠΆΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ (GO:0098609 - cell-cell adhesion), ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ DSC1, DSC3 ΠΈ DSG1. Π‘Π½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π΄Π΅ΡΠΌΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ°Π΄Π³Π΅ΡΠΈΠ½ΠΎΠ² Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎ Π΄Π»Ρ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ, ΡΠ°Π·Π²ΠΈΠ²Π°ΡΡΠΈΡ
ΡΡ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΡ
ΠΎΡΠ³Π°Π½ΠΎΠ², ΡΠ»ΠΈΠ·ΠΈΡΡΡΡ
ΠΎΠ±ΠΎΠ»ΠΎΡΠ΅ΠΊ, ΠΊΠΎΠΆΠΈ. ΠΡΠΈΠΌΠ΅Π½Π΅Π½Π½ΡΠΉ Π² ΡΠ°Π±ΠΎΡΠ΅ ΡΠΏΠΎΡΠΎΠ± ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ² ΠΠ°Π‘Π°Π’ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΠ΅ Π³Π΅Π»Ρ Π² Π΄Π²Π° ΡΠ°Π·Π° Π±ΠΎΠ»ΡΡΠ΅ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΎΠ±ΡΠ°Π·ΡΠ°ΠΌΠΈ ΠΠ°Π‘Π°Π’, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π½ΡΡΡΠΌΠΈ ΠΎΡΠΌΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΡΠΎΠΊΡ ΠΈ ΡΠ°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΡ ΡΡΠΈΠΏΡΠΈΠ½ΠΎΠΌ Π² ΡΠ°ΡΡΠ²ΠΎΡΠ΅
ΠΠΎΡΡΠΎΡΠ½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΌΠΎΡΠΈ Π·Π΄ΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ Ρ 520-ΡΡΡΠΎΡΠ½ΠΎΠΉ ΠΈΠ·ΠΎΠ»ΡΡΠΈΠ΅ΠΉ
Purpose of the study was to track permanent proteins of urine proteome in the 520-day isolation experiment at the IBMP Ground-Based Test Facility with controlled environmental parameters. Object of the investigation was urine sampled from 6 normal male subjects at the age of 25 to 37 years. Second morning aliquots were gathered during baseline data collection, on days 50, 93, 124, 153, 180, 251, 274, 303, 330, 371, 400 and 427 of isolation, and in 7 days after its completion. Samples were subject to chromatography-mass spectrometry; results were analyzed with the help of bioinformatics resources. The following 7 permanent proteins were observed in urine over the entire length of the investigation: epidermal growth factor, polymer immunoglobulin receptor, plasma serine protease inhibitor, protein AMBP, keratin, type II cytoskeletal 1, collagen alpha-1 (vi) chain, serum albumin
Environmental-Toxicological Characteristics of Waters and Their Sources at Magnitogorsk With the Its Iron and Steel Industry
This study summarizes the information necessary to characterize and assess the quality of drinking and industrial water supply in industrial centers with metallurgical engineering and provides information about the pollution impact on the natural environment. The study shows the influence of air pollution, of the soil pollution on the environment of water objects; it also demonstrates the role of the quality of water supply for establishing a higher risk of health problems for children
Synthesis and photochromism of a series of new 2-unsubstituted 3-(2-benzylbenzoyl)quinolin-4(1<i>H)-ones
Synthesis and characterization of a series of new 2-unsubstituted 3-(2-benzylbenzoyl)quinolin-4(1H)-ones is described. In addition to their potential biological activity, these compounds exhibit photoreversible photochromic properties in anaerobic conditions. Using the 1 and 2D NMR techniques we demonstrated that the coloration occurred owing to the formation of fluorescent 5a,6-dihydrobenzo[b]acridin-(5H)-ones. The photoreaction is stereoselective and the S,R-isomers are the major products (83%) whereas the S,S-isomers are the minor (6%). In addition, the irreversible formation of two oxidation products, 3-(2-benzoylbenzoyl)quinolin-4(1H)-ones, and acridin-12(5H)-one derivatives was observed in the presence of air
ΠΡΠΎΡΠ΅ΠΎΠΌΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π²ΠΎΡΡΠΈΠ½ Ρ ΠΎΡΠΈΠΎΠ½Π° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΡΠΈ Π°Π½ΡΠΌΠ±ΡΠΈΠΎΠ½ΠΈΠΈ
In this study, the proteomic approach based on high performance liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) and bioinformatics analysis were applied to identify differentially abundant proteins in chorionic villus samples (CVS) from women with blighted ovum and normal pregnancy. We identified about 600 proteins in the solubilized fraction of CVS. Comparative proteomic analysis revealed differences in the content (Average Normalized Abundances) of 187 proteins in blighted ovum. These included 134 down-regulated proteins and 53 up-regulated proteins. According to bioinformatics analysis these proteins participate in a variety of metabolic processes, including alcohol and tricarboxylic acid metabolism, response to endoplasmic reticulum stress, small molecular catabolic process, cellular respiration, and others. Proteins that demonstrated growing content in blighted ovum were mainly encoded by genes located on chromosomes 7 and 16 whereas proteins which demonstrated reducing abundance were mainly encoded by genes located on chromosomes 1, 2, and 11. We also revealed changes in the content of proteins encoded by genes located on the human chromosome 18; they are involved in apoptotic and drug metabolic processes with an important role in early pregnancy loss. Our pilot results demonstrate the efficiency of the LC-MS/MS approach for detecting the differences at the qualitative and semi-quantitative levels in the protein profiles of the CVS at anembryonic pregnancy compared to normal gestation. We conclude that globally profiled and differentially regulated proteins of CVS are helpful in obtaining molecular insights into biological processes of the pregnancy pathology.Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΡΠΉ Π½Π° ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ, ΡΠΎΠ²ΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠΉ Ρ ΡΠ°Π½Π΄Π΅ΠΌΠ½ΠΎΠΉ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠ΅ΠΉ (LC-MS/MS), ΠΈ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· Π±ΡΠ»ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½Ρ Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌ ΠΏΡΠΎΡΠΈΠ»Π΅ΠΌ Π²ΠΎΡΡΠΈΠ½ Ρ
ΠΎΡΠΈΠΎΠ½Π° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΡΠΈ Π°Π½ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ. ΠΡΠ΅Π³ΠΎ Π² ΡΠΎΠ»ΡΠ±ΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΠΊΡΠΈΠΈ Ρ
ΠΎΡΠΈΠΎΠ½Π° Π±ΡΠ»ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΎΠΊΠΎΠ»ΠΎ 600 Π±Π΅Π»ΠΊΠΎΠ². Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΠΎΠ³ΠΎ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΡ Progenesis LS-MS ΠΏΠΎΠΊΠ°Π·Π°Π» ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ 187 Π±Π΅Π»ΠΊΠΎΠ² ΠΏΡΠΈ Π°Π½ΡΠΌΠ±ΡΠΈΠΎΠ½ΠΈΠΈ, ΠΈΠ· Π½ΠΈΡ
134 Π±Π΅Π»ΠΊΠ° ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΈ 53 Π±Π΅Π»ΠΊΠ° β ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ (average normalized abundances). ΠΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ, ΡΡΠΎ ΡΡΠΈ Π±Π΅Π»ΠΊΠΈ ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡ ΡΡΠ°ΡΡΠΈΠ΅ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
, Π²ΠΊΠ»ΡΡΠ°Ρ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Ρ ΠΈ ΡΡΠΈΠΊΠ°ΡΠ±ΠΎΠ½ΠΎΠ²ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ, Π² ΡΠ΅Π°ΠΊΡΠΈΡΡ
, Π°ΡΡΠΎΡΠΈΠΈΡΡΠ΅ΠΌΡΡ
ΡΠΎ ΡΡΡΠ΅ΡΡΠΎΠΌ ΡΠ½Π΄ΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠΈΠΊΡΠ»ΡΠΌΠ°, ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
ΠΊΠ°ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°, ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΌ Π΄ΡΡ
Π°Π½ΠΈΠΈ ΠΈ Π΄ΡΡΠ³ΠΈΡ
. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΡ
Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΠΎΠΉ 18 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡΠΈΡ
ΡΡΠ°ΡΡΠΈΠ΅ Π² Π°ΠΏΠΎΠΏΡΠΎΠ·Π΅ ΠΈ ΡΠ΅Π°ΠΊΡΠΈΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΡΠ΅Π΄ΡΡΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
, ΠΈΠ³ΡΠ°ΡΡΠΈΡ
Π²Π°ΠΆΠ½ΡΡ ΡΠΎΠ»Ρ ΠΏΡΠΈ ΠΏΠΎΡΠ΅ΡΡΡ
Π² ΡΠ°Π½Π½ΠΈΠ΅ ΡΡΠΎΠΊΠΈ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ. ΠΠ°ΡΠΈ ΠΏΡΠ΅Π΄Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΡΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ LC-MS/MS Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΈ ΠΏΠΎΠ»ΡΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΈΠ»Ρ Π²ΠΎΡΡΠΈΠ½ Ρ
ΠΎΡΠΈΠΎΠ½Π° ΠΏΡΠΈ Π°Π½ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ. Π‘Π΄Π΅Π»Π°Π½ Π²ΡΠ²ΠΎΠ΄ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ ΡΠΈΡΠΎΠΊΠΎΠΌΠ°ΡΡΡΠ°Π±Π½ΠΎΠ΅ ΠΏΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΠΌΡΡ
Π±Π΅Π»ΠΊΠΎΠ² Π²ΠΎΡΡΠΈΠ½ Ρ
ΠΎΡΠΈΠΎΠ½Π° ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½ΠΎ Π΄Π»Ρ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ², ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠΈΡ
ΠΏΡΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ