Metabarcoding dietary analysis in the insectivorous bat Nyctalus leisleri and implications for conservation

In this study, we aim to uncover diet preferences for the insectivorous bat Nyctalus leisleri (Leisler's bat, the lesser noctule) and to provide recommendations for conservation of the species, based on the analysis of prey source habitats. Using a novel guano trap, we sampled bat faeces at selected roosts in a forest in Germany and tested two mitochondrial markers (COI and 16S) and three primer pairs for the metabarcoding of bat faecal pellets.We found a total of 17 arthropod prey orders comprising 358 species in N. leisleri guano. The most diverse orders were Lepidoptera (126 species), Diptera (86 species) and Coleoptera (48 species), followed by Hemiptera (28 species), Trichoptera (16 species), Neuroptera (15 species) and Ephemeroptera (10 species), with Lepidoptera species dominating in spring and Diptera in summer. Based on the ecological requirements of the most abundant arthropod species found in the bat guano, we propose some recommendations for the conservation of N. leisleri that are relevant for other insectivorous bat species

Auswirkungen des Allgemeinen Gleichbehandlungsgesetzes (AGG) auf das Personalmanagement. Eine explorative Untersuchung im Rahmen eines Studienprojekts

Langen H. Auswirkungen des Allgemeinen Gleichbehandlungsgesetzes (AGG) auf das Personalmanagement. Eine explorative Untersuchung im Rahmen eines Studienprojekts. Diskussionspapier / Fakultät für Wirtschaftswissenschaften, Universität Bielefeld. Bielefeld: Universität Bielefeld; 2008.Ein halbes Jahr nach Inkrafttreten des Allgemeinen Gleichbehandlungsgesetzes (AGG) wurde mittels einer qualitativen Befragung untersucht, wie sich Unternehmen in der Region Ostwestfalen-Lippe auf die Einführung des Gesetzes vorbereitet haben und welche ersten Erfahrungen es aus Sicht von Personalverantwortlichen gibt

Auswirkungen des Allgemeinen Gleichbehandlungsgesetzes (AGG) auf das Personalmanagement. Eine explorative Untersuchung im Rahmen eines Studienprojekts

Langen H. Auswirkungen des Allgemeinen Gleichbehandlungsgesetzes (AGG) auf das Personalmanagement. Eine explorative Untersuchung im Rahmen eines Studienprojekts. Diskussionspapier / Fakultät für Wirtschaftswissenschaften, Universität Bielefeld. Bielefeld: Universität Bielefeld; 2008.Ein halbes Jahr nach Inkrafttreten des Allgemeinen Gleichbehandlungsgesetzes (AGG) wurde mittels einer qualitativen Befragung untersucht, wie sich Unternehmen in der Region Ostwestfalen-Lippe auf die Einführung des Gesetzes vorbereitet haben und welche ersten Erfahrungen es aus Sicht von Personalverantwortlichen gibt

Individualisierung der Arbeitszeit mit Langzeitkonten: eine explorative Studie

Langen H. Individualisierung der Arbeitszeit mit Langzeitkonten: eine explorative Studie. Personalwirtschaft. Vol 54. Hamburg: Verlag Dr. Kovac; 2016

Supplementary material 2 from: Bourlat SJ, Koch M, Kirse A, Langen K, Espeland M, Giebner H, Decher J, Ssymank A, Fonseca VG (2023) Metabarcoding dietary analysis in the insectivorous bat Nyctalus leisleri and implications for conservation. Biodiversity Data Journal 11: e111146. https://doi.org/10.3897/BDJ.11.e111146

ASV table for the guano sample

Arthropod diversity in the alpine tundra using metabarcoding: Spatial and temporal differences in alpha‐ and beta‐diversity

Abstract All ecosystems face ecological challenges in this century. Therefore, it is becoming increasingly important to understand the ecology and degree of local adaptation of functionally important Arctic‐alpine biomes by looking at the most diverse taxon of metazoans: the Arthropoda. This is the first study to utilize metabarcoding in the Alpine tundra, providing insights into the effects of micro‐environmental parameters on alpha‐ and beta‐diversity of arthropods in such unique environments. To characterize arthropod diversity, pitfall traps were set at three middle‐alpine sampling sites in the Scandinavian mountain range in Norway during the snow‐free season in 2015. A metabarcoding approach was then used to determine the small‐scale biodiversity patterns of arthropods in the Alpine tundra. All DNA was extracted directly from the preservative EtOH from 27 pitfall traps. In order to identify the controlling environmental conditions, all sampling locations were equipped with automatic data loggers for permanent measurement of the microenvironmental conditions. The variables measured were: air temperature [°C] at 15 cm height, soil temperature [°C] at 15 cm depth, and soil moisture [vol.%] at 15 cm depth. A total of 233 Arthropoda OTUs were identified. The number of unique OTUs found per sampling location (ridge, south‐facing slope, and depression) was generally higher than the OTUs shared between the sampling locations, demonstrating that niche features greatly impact arthropod community structure. Our findings emphasize the fine‐scale heterogeneity of arctic–alpine ecosystems and provide evidence for trait‐based and niche‐driven adaptation. The spatial and temporal differences in arthropod diversity were best explained by soil moisture and soil temperature at the respective locations. Furthermore, our results show that arthropod diversity is underestimated in alpine‐tundra ecosystems using classical approaches and highlight the importance of integrating long‐term functional environmental data and modern taxonomic techniques into biodiversity research to expand our ecological understanding of fine‐ and meso‐scale biogeographical patterns

Supplementary material 4 from: Bourlat SJ, Koch M, Kirse A, Langen K, Espeland M, Giebner H, Decher J, Ssymank A, Fonseca VG (2023) Metabarcoding dietary analysis in the insectivorous bat Nyctalus leisleri and implications for conservation. Biodiversity Data Journal 11: e111146. https://doi.org/10.3897/BDJ.11.e111146

Arthropod prey species in the guano for all N. leisleri sample