Using field-based entomological research to promote awareness about forest ecosystem conservation

Interactions between plants, insect herbivores and associated predators represent the majority of terrestrial biodiversity. Insects are vital food sources for many other organisms and provide important ecosystem functions and services including pollination, waste removal and biological control. We propose a complete and reproducible education programme to guide students to understand the importance of managing and conserving forest ecosystems in their region through the study of insect ecology and natural history. Our programme involved lectures, workshops and field surveys of insects with a group of 60 high school students in Eastern Africa (Ethiopia). It addresses the key stages of an entomological research project including: 1) general entomological knowledge and understanding the role of insects in terrestrial diversity and in ecosystem functioning and services; (2) the proposal of simple research questions including hypothesis development and evaluation using scientific literature, 3) fieldwork using different types of light traps; 4) sorting and identification of the insect orders using simple diagnostic keys and illustrated plates; 5) analysing and interpreting the results and 6) demonstrating findings to peers and a public audience. Identifying insects, exploring their natural history and understanding their functions in the field bring the students towards a better understanding and awareness of the importance of forest ecosystem conservation

Scientists' warning on climate change and insects

Climate warming is considered to be among the most serious of anthropogenic stresses to the environment, because it not only has direct effects on biodiversity, but it also exacerbates the harmful effects of other human-mediated threats. The associated consequences are potentially severe, particularly in terms of threats to species preservation, as well as in the preservation of an array of ecosystem services provided by biodiversity. Among the most affected groups of animals are insects—central components of many ecosystems—for which climate change has pervasive effects from individuals to communities. In this contribution to the scientists' warning series, we summarize the effect of the gradual global surface temperature increase on insects, in terms of physiology, behavior, phenology, distribution, and species interactions, as well as the effect of increased frequency and duration of extreme events such as hot and cold spells, fires, droughts, and floods on these parameters. We warn that, if no action is taken to better understand and reduce the action of climate change on insects, we will drastically reduce our ability to build a sustainable future based on healthy, functional ecosystems. We discuss perspectives on relevant ways to conserve insects in the face of climate change, and we offer several key recommendations on management approaches that can be adopted, on policies that should be pursued, and on the involvement of the general public in the protection effort

Data from: Taxonomic and functional composition of arthropod assemblages across contrasting Amazonian forests

Arthropods represent most of global biodiversity, with the highest diversity found in tropical rainforests. Nevertheless, we have a very incomplete understanding of how tropical arthropod communities are assembled. We conducted a comprehensive mass-sampling of arthropod communities within three major habitat types of lowland Amazonian rainforest, including terra firme clay, white-sand, and seasonally-flooded forests in Peru and French Guiana. We examined how taxonomic and functional composition (at the family level) differed across these habitat types in the two regions. The overall arthropod community composition exhibited strong turnover among habitats and between regions. In particular, seasonally-flooded forest habitats of both regions comprised unique assemblages. Overall, 17.7% (26 of 147) of arthropod families showed significant preferences for a particular habitat type. We present a first reproducible arthropod functional classification among the 147 taxa based on similarity among 21 functional traits describing feeding source, major mouthparts and microhabitats inhabited by each taxon. We identified seven distinct functional groups whose relative abundance contrasted strongly across the three habitats, with sap and leaf feeders showing higher abundances in terra firme clay forest. Our novel arthropod functional classification provides an important complement to link these contrasting patterns of composition to differences in forest functioning across geographic and environmental gradients. This study underlines that both environment and biogeographical processes are responsible for driving arthropod taxonomic composition while environmental filtering is the main driver of the variance in functional composition

Data from: Evolution of Manduca sexta hornworms and relatives: biogeographical analysis reveals an ancestral diversification in Central America

The hawkmoth genus Manduca is a diverse group of very large, conspicuous moths that has served as an important model across many biological disciplines. Two species in particular, the tobacco hornworm (Manduca sexta) and the tomato hornworm (Manduca quinquemaculatus) have been researched extensively. Studies across biological fields have referred to these two species as being closely related or even sister species, but the extent to which these two model organisms are related remains largely unknown. We conducted a comprehensive multi-gene phylogenetic analysis of Manduca, based on both an ML and Bayesian framework, which resulted in a monophyletic Manduca but only when two other genera, Dolba and Euryglottis are included. We tentatively conclude that the sister group to Manduca sexta comprises the Caribbean M. afflicta and M. johanni, and the sister lineage to this clade includes M. quinquemaculatus and the Hawaiian M. blackburni. Thus, M. sexta and M. quinquemaculatus are closely related, but are not sister species. Biogeographical analyses reveal an ancestral center of diversification in Central America, and Manduca appears to have subsequently colonized North and South America. Our phylogeny provides an important foundation for comparative studies of two model organisms and their relatives

Abundance of 147 taxa of tropical arthropods

Arthropod communities were sampled weekly in the twelve permanent plots from August to November 2010 in French Guiana and between May and September 2011 in Peru, corresponding to drier periods in each region. We employed a multiple trapping method composed of two types of flight interception trap (FIT), the Malaise trap (MT) and the Windowpane trap (WT); and two types of attractive traps: the Fruit trap (FT) and the Automatic Light trap (ALT

Abundance, occurrence and time series: long-term monitoring of social insects in a tropical rainforest

The magnitude of worldwide insect decline is hotly debated, with multiple examples of stable or increasing insect populations. In addition, time series data for tropical insects are scarce, notably in rainforests where insect diversity is poorly known but reaches a peak. Despite social insects (ants, termites, bees and allies) being key organisms in these habitats, long-term monitoring data for these groups are crucially lacking. For many of these insects, the difficulty of locating nests in rainforests could be one reason. In this context, species occurrence in samples is often used as a surrogate for abundance to evaluate species distribution in space/time, but the loss of information is difficult to assess. In a tropical rainforest in Panama, we employed various sampling methods to examine the time series of seven insect assemblages with differing degrees of sociality: termite workers and soldiers, termite alates, bess beetles, litter ant workers, army ant alates, orchid bees, and nocturnal sweat bees. We used five community variables and six models related to occurrence and abundance, to test for significant trends in assemblages over a 13-year period (2009–2021). While assemblages of bess beetles increased, those of termite workers and soldiers, army ant alates, and orchid bees remained relatively stable. Termite alate, litter ant worker, and nocturnal bee assemblages showed signs of decline, demonstrating the need for monitoring distinct assemblages. Significant trends in generalized additive mixed models (GAMM) were observed in three out of five assemblages that could be tested. Our study indicates that trends in assemblages may be more informatively reported with abundance than with occurrence. We recommend (1) monitoring multiple insect assemblages as ecological indicators responsible for diverse ecosystem services; and (2) reporting species richness, changes in faunal composition, occurrence, and, when possible, using time-explicit analyses (such as GAMM models) for evaluating population trends over time

Appendix H. A table of measurements of leaf size and physical and chemical defense traits for each ecotype.

A table of measurements of leaf size and physical and chemical defense traits for each ecotype

Appendix F. A table showing variation in herbivore communities and relative abundance of leaf secondary metabolites.

A table showing variation in herbivore communities and relative abundance of leaf secondary metabolites