Pharmaco-therapeutic profile of Drakshadi Phanta

Drakshadi Phanta is mentioned in Astanga Hridaya Chikitsasthana for Chardi. Garbhini Chardi is one of the Garbhopadravas caused due to Vatavaigunya leading to Agnivaishamya. The drugs here are having Deepana, Pachana, Vatahara, Chardighna, Grahi & Sheeta Veerya properties, may be beneficial to control Chardi. Vomiting in pregnancy is seen mainly due to metabolic disturbances and also due to carbohydrate starvation. The metabolism & absorption of medicine in Phanta form starts from the mouth itself because of presence of glucose, Fructose etc. Draksha is Deepaka, Pachaka, Ruchya Rasayana, Hrudya with Madhu and Sita helps correcting the metabolism and supplementing carbohydrates also. Hence controls the vomiting along with correction of dehydration

3-{[6-(4-Chlorophenyl)imidazo[2,1-b][1,3,4]thiadiazol-2-yl]methyl}-1,2-benzoxazole

In the title compound, C18H11ClN4OS, the benzisoxazole and imidazothiadiazole rings are inclined at an angle of 23.81 (7)degrees with respect to each other. The imidazothiadiazole and chlorophenyl rings make a dihedral angle of 27.34 (3)degrees. In the crystal, intermolecular C-H center dot center dot center dot N interactions generate a chain along the c axis and C-H center dot center dot center dot O interactions form centrosymmetric dimers resulting in an R-2(2)(26) graph-set motif. Moreover, the C-H center dot center dot center dot N and S center dot center dot center dot N [3.206 (4) angstrom] interactions links the molecules into R(7) ring motifs. The packing is further stabilized by pi-pi stacking interactions between the thiadiazole rings with a shortest centroid-centroid distance of 3.497 (3) angstrom. In addition, C-H center dot center dot center dot pi interactions are observed in the crystal structur

6-(4-Bromo­phen­yl)-2-(4-fluoro­benz­yl)imidazo[2,1-b][1,3,4]thia­diazole

In the title compound, C17H11BrFN3S, the imidazothia­diazole and bromo­phenyl rings are individually almost planar, with maximum deviations of 0.0215 (4) and 0.0044 (4) Å, respectively, and are inclined at an angle of 27.34 (3)° with respect to each other. The dihedral angle between the mean planes of the fluoro­benzyl and imidazothia­diazole rings is 79.54 (3)°. The crystal structure is stabilized by inter­molecular C—H⋯N inter­actions resulting in chains of mol­ecules along the b axis

Adenovirus Gene Transfer to Amelogenesis Imperfecta Ameloblast-Like Cells

To explore gene therapy strategies for amelogenesis imperfecta (AI), a human ameloblast-like cell population was established from third molars of an AI-affected patient. These cells were characterized by expression of cytokeratin 14, major enamel proteins and alkaline phosphatase staining. Suboptimal transduction of the ameloblast-like cells by an adenovirus type 5 (Ad5) vector was consistent with lower levels of the coxsackie-and-adenovirus receptor (CAR) on those cells relative to CAR-positive A549 cells. To overcome CAR -deficiency, we evaluated capsid-modified Ad5 vectors with various genetic capsid modifications including “pK7” and/or “RGD” motif-containing short peptides incorporated in the capsid protein fiber as well as fiber chimera with the Ad serotype 3 (Ad3) fiber “knob” domain. All fiber modifications provided an augmented transduction of AI-ameloblasts, revealed following vector dose normalization in A549 cells with a superior effect (up to 404-fold) of pK7/RGD double modification. This robust infectivity enhancement occurred through vector binding to both αvβ3/αvβ5 integrins and heparan sulfate proteoglycans (HSPGs) highly expressed by AI-ameloblasts as revealed by gene transfer blocking experiments. This work thus not only pioneers establishment of human AI ameloblast-like cell population as a model for in vitro studies but also reveals an optimal infectivity-enhancement strategy for a potential Ad5 vector-mediated gene therapy for AI

Editorial

Archives are sites of exploration and discovery for all kinds of practices. They are also reinforced structures. Whether as a library of manuscripts, museum store or personal collection, the ‘archive-as-repository’ catalogues and categorizes, houses and buries, its items. Bringing the contents of an archive to life requires that one ignite what is dormant so as to draw archival materials out into the space of the world to be received and experienced in new ways. Designed to stimulate collaborative conversations and exchanges, in and around the archive, with a view to presenting new approaches to archival experiences, and with them, styles of writing that resonate with the ‘archival’ as a concept and as a practice, this guest-edited issue expands the field of the archive to incorporate a variety of different practitioner perspectives. Whether through animation, art education, contemporary art, costume, creative writing, information retrieval studies, performance, sculpture, sound and textiles, re-writing the archive from these positions can inform how historical and material remnants of the past may be re-thought in creative practice

3-{[5-(4-Bromophenyl)imidazo[2,1-b][1,3,4]thiadiazol-2-yl]methyl}-1,2-benzoxazole

In the title compound, C18H11BrN4OS, the imidazothiadiazole and benzisoxazole rings are individually planar with maximum deviations of 0.025 (3) 0.015 (4) Å, respectively, and are inclined at an angle of 23.51 (7)° with respect to each other. The planes of the imidazothiadiazole and bromophenyl rings are inclined at an angle of 27.34 (3)°. In the crystal, intermolecular C—H...N interactions result in chains of molecules along the b and c axes. Moreover, C—H...O interactions result in centrosymmetric head-to-head dimers with R22(24) graph-set motifs. The molecular packing is further stabilized by π–π stacking interactions between the imidazole rings with a shortest centroid–centroid distance of 3.492 (3) Å. In addition, C—H...π interactions are observed in the crystal structure

Evaluation of recently developed techniques for estimating diet composition in browsing herbivores.

Accuracy of estimates of botanical composition of herbivore diet may be improved through the use of recently developed techniques (alkane analysis, remote controlled oesophageal fistula valve). Three Boer goats allowed free choice of five forage species were used to compare seven methods of estimating diet composition with observed diet composition. The evaluated techniques either monitored intake [bite-count (IT1), bite mass corrected bite-count (IT2), relative bite mass corrected bite-count (IT3), micro-histological analysis of oesophageal extrusa collected at hourly intervals (IT4)] or faeces [alkane analysis (FT1), micro-histological faecal analysis (FT2), and in vitro corrected, micro-histological faecal analysis (FT3)]. Intake techniques had higher Kulczynski's similarity coefficients with measured values (70.7, 70.2, 65.5, and 78.6 respectively for IT1, IT2, IT3 and IT4) than did faecal techniques (58.9, 58.0 and 51.2 respectively for FT1, FT2 and FT3). Compared to IT2 and IT3, IT4 provided estimates of diet composition that was more similar to measured diet composition in terms of Kulczynski's similarity coefficients. However, estimates of diet composition were significantly different from measured values in 60% of cases for IT4 but only 40% of cases for IT2 and IT3. It is concluded that bite-count techniques (IT1 - IT3) can give as accurate estimate of diet composition as the more costly and technically demanding micro-histological analysis of oesophageal extrusa (IT4)