Survival of thermophilic spore-forming bacteria in a 90+ year old milk powder from Ernest Shackelton's Cape Royds Hut in Antarctica

Milk powder taken to Antarctica on Shackelton's British Antarctic Expedition in 1907 was produced in New Zealand by a roller drying process in the first factory in the world dedicated to this process. Thermophilic bacilli are the dominant contaminants of modern spray-dried milk powders and the 1907 milk powder allows a comparison to be made of contaminating strains in roller-dried and spray-dried powders. Samples of milk powder obtained from Shackelton's Hut at Cape Royds had low levels of thermophilic contamination (<500 cfu ml−1) but the two dominant strains (Bacillus licheniformis strain F and Bacillus subtilis) were typical of those found in spray-dried powders. Soil samples from the floor of the hut also contained these strains, whereas soils distant from the hut did not. Differences in the RAPD profiles of isolates from the milk powder and the soils suggest that contamination of the milk from the soil was unlikely. It is significant that the most commonly encountered contaminant strain in modern spray-dried milk (Anoxybacillus flavithermus strain C) was not detected in the 1907 sample

Gold mining, indigenous land claims and conflict in Guyana's hinterland

In recent decades, Guyana’s gold-rich interior has been the location of numerous, mostly low-latent, conflicts. In each case, groups of Afro and Indo-Guyanese originating from the country’s coastal cities and towns – popularly referred to as ‘Coast Landers’ – have clashed with indigenous Amerindians over control of remote parcels of land containing gold deposits. Each appears to have a valid argument in support of its position: the former contend that they are legally entitled to work these lands, having obtained the requisite permits from the central government to mine for gold, whilst the latter maintain that such decisions constitute a breach of their human rights, and draw attention to key legislation in support of their case. This article broadens understanding of the dynamics of these conflicts by reflecting more critically on the arguments presented by both parties. Drawing heavily on research conducted in Mahdia-Campbelltown, one location where frictions between Coast Lander mining groups and Amerindians are particularly serious, it is argued that these disputes are not about control of gold riches as is popularly believed but rather a product of deeply-rooted ethnic tensions between these parties

An unusual rash heralding pleomorphic transformation of Mantle Cell Lymphoma

Open Access via Wiley publishing agreement.Peer reviewedPublisher PD

Correction: Bioinformatics and Multiepitope DNA Immunization to Design Rational Snake Antivenom

Correction: Bioinformatics and Multiepitope DNA Immunization to Design Rational Snake Antiveno

Bioinformatics and Multiepitope DNA Immunization to Design Rational Snake Antivenom

BACKGROUND: Snake venom is a potentially lethal and complex mixture of hundreds of functionally diverse proteins that are difficult to purify and hence difficult to characterize. These difficulties have inhibited the development of toxin-targeted therapy, and conventional antivenom is still generated from the sera of horses or sheep immunized with whole venom. Although life-saving, antivenoms contain an immunoglobulin pool of unknown antigen specificity and known redundancy, which necessitates the delivery of large volumes of heterologous immunoglobulin to the envenomed victim, thus increasing the risk of anaphylactoid and serum sickness adverse effects. Here we exploit recent molecular sequence analysis and DNA immunization tools to design more rational toxin-targeted antivenom. METHODS AND FINDINGS: We developed a novel bioinformatic strategy that identified sequences encoding immunogenic and structurally significant epitopes from an expressed sequence tag database of a venom gland cDNA library of Echis ocellatus, the most medically important viper in Africa. Focusing upon snake venom metalloproteinases (SVMPs) that are responsible for the severe and frequently lethal hemorrhage in envenomed victims, we identified seven epitopes that we predicted would be represented in all isomers of this multimeric toxin and that we engineered into a single synthetic multiepitope DNA immunogen (epitope string). We compared the specificity and toxin-neutralizing efficacy of antiserum raised against the string to antisera raised against a single SVMP toxin (or domains) or antiserum raised by conventional (whole venom) immunization protocols. The SVMP string antiserum, as predicted in silico, contained antibody specificities to numerous SVMPs in E. ocellatus venom and venoms of several other African vipers. More significantly, the antiserum cross-specifically neutralized hemorrhage induced by E. ocellatus and Cerastes cerastes cerastes venoms. CONCLUSIONS: These data provide valuable sequence and structure/function information of viper venom hemorrhagins but, more importantly, a new opportunity to design toxin-specific antivenoms—the first major conceptual change in antivenom design after more than a century of production. Furthermore, this approach may be adapted to immunotherapy design in other cases where targets are numerous, diverse, and poorly characterized such as those generated by hypermutation or antigenic variation

An external sensing system in Plasmodium falciparum-infected erythrocytes

Additional file 4. ItG-pRBC were treated with 10 ng/ml TNF for 60, 30, 15 and 5 min and the cellular proteins were extracted and separated by SDS-PAGE, followed by Western blot probed with anti-HSP90 and AB4 antibodies. The 90kDa protein recognized by the two antibodies showed similar expression patterns

Formalizing artisanal and small-scale mining in Mozambique: concerns, priorities and challenges

This paper reflects critically on efforts made to formalize artisanal and small-scale mining (ASM) – low-tech, labour-intensive mineral extraction and processing – in Mozambique. Drawing on findings from interviews with policymakers, representatives from ASM associations and consultations with 200 individual miners, the paper captures the details of the country’s ASM formalization experience. Findings reveal that despite showing considerable promise at first, the drive to formalize ASM in Mozambique, which spans three decades, has lost considerable momentum. A bureaucratic licensing scheme, overlapping responsibilities at the Estatuto Orgânico do Ministério dos Recursos Minerais e Energia (MIREME), and a shortage of information about miners have contributed to this slowdown. The themes underpinning the efforts to formalize ASM in Mozambique are not new but the case itself has its own unique nuances and storylines

Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis

Streptococcus pneumoniae is responsible for diseases causing major global public health problems, including meningitis, pneumonia and septicaemia. Despite recent advances in antimicrobial therapy, pneumococcal meningitis remains a life-threatening disease. Furthermore, long-term sequelae are a major concern for survivors. Hence, a better understanding of the processes occurring in the central nervous system is crucial to the development of more effective management strategies. We used mass spectrometry based quantitative proteomics to identify protein changes in cerebrospinal fluid from children with Streptococcus pneumoniae infection, compared with children admitted to hospital with bacterial meningitis symptoms but negative diagnosis. Samples were analysed, by label free proteomics, in two independent cohorts (cohort 1: cases (n = 8) and hospital controls (n = 4); cohort 2: cases (n = 8), hospital controls (n = 8)). Over 200 human proteins were differentially expressed in each cohort, of which 65% were common to both. Proteins involved in the immune response and exosome signalling were significantly enriched in the infected samples. For a subset of proteins derived from the proteome analysis, we corroborated the proteomics data in a third cohort (hospital controls (n = 15), healthy controls (n = 5), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics findings. Proteomics data are available via ProteomeXchange with identifier PXD004219

(Re)discovering the Gaulcross Hoard

Acknowledgements We would like to thank Charles and Helen Gray for permitting access to the land and for support in the excavation of the hoard. Thanks also to Bruce Mann and the Aberdeenshire Council Archaeology Service for advice and supporting the radiocarbon dating. Fraser Hunter and Tanja Romankiewicz assisted during a very cold excavation. Fraser and Alice Blackwell kindly read and commented on drafts of this paper. The fieldwork was funded through a donation to the University of Aberdeen's Development Trust and undertaken as part of the Northern Picts project, in association with the Tarbat Discovery Centre.Peer reviewedPostprin