Cartographie sectorielle et dynamique de la plaine alluviale du Rhône suisse (Tourtemagne-Sierre) depuis la fin du Petit Age Glaciaire

National audienc

Cartographie sectorielle du paléoenvironnement de la plaine alluviale du Rhône suisse depuis la fin du Petit Age Glaciaire : la métamorphose fluviale de Viège à Rarogne et de Sierre à Sion.

National audienc

Children's Independent Mobility: Survey in French Brittany (2011)

The study contributes to an international survey carried out in 16 countries: Australia, Brazil, Denmark, England, Finland, France, Germany, Ireland, Israel, Italy, Japan, Norway, Portugal, South Africa, Sri Lanka, Sweden(Shaw & Watson, 2010). The international survey was initiated by the Political Study Institute of London as an extended geographical replication of the original study of Hillman & al (1990).The general objective of this study is to investigate how children's independent mobility develops between age 7-15. The degree of independent mobility is assessed through the examination of children's statements about six licences related to outside trips without adult supervision. The objective is to provide a detailed picture of the current state of independent mobility in primary and secondary school children of French Brittany (North-West of France). In this respect, the study contributes to an international survey carried out in 16 countries (Shaw & Watson, 2010). The international survey was initiated by the Political Study Institute of London as an extended geographical replication of the original study of Hillman & al (1990). Method A total of 947 children participated in the French survey. Specifically, in primary school 484 children--48,8% girls, 51,2% boys--filled up the questionnaire, age ranging from 6 to 12, mean = 8,79 (sd =1,27). In secondary school 463 young people--49,7% girls, 50,3% boys--responded to the questionnaire, age ranging from 10 to 16, mean = 12,9 (sd =1,29). The survey was conducted in different types of living environment varying in relation to the size and density of the dwelling area. Five types of areas were considered: (1) inner district of a major city, (2) suburban area of a major city, (3) small town, (4) rural market town and (5) rural area. In the French survey, the five types of areas were selected in the same region, namely the district (département) of Ille-et-Vilaine in French Brittany. Therefore, the survey design, which gathered data from different types of areas, was likely to provide a comprehensive picture of the independent mobility of the children living in that particular region. Children's independent mobility was assessed through the examination of six licences: (1) Licence to cross roads alone, (2) Licence to travel to and from school alone, (3) Licence to go on their own to places other than school, (4) Licence to cycle on main roads, (5) Licence to use buses, (6) Licence to go out after dark. Results The comparison of the licences granted by parents to Psc and to Ssc reveals marked difference for all the six licences. This denotes important changes in the parental attitude towards these children's independent mobility within the considered age range. Interestingly the hierarchy of the six licences is almost the same for the two groups. For both groups the licence to cross main roads is the most frequently granted, whereas licence to go out after dark is the least granted. Even in the secondary school, only a few French children are allowed to go out after dark. The analysis of the children's responses also puts forward that independent mobility develop markedly after 11 years when children are in secondary school. Specifically, four of the six licences are held by a large majority of the secondary school children: to go to other places than school on their own, to cross main roads, to use public transport, and to cycle on main roads. However, only one third of these older children declared to go to and from school on their own. This result is probably due to the size of the secondary school catchment areas which were particularly large in four of the five survey areas. Therefore, the distances from the children's homes to secondary school constrained the older children to use the school bus or to be driven by their parents. The primary school children's independent mobility is particularly restricted; four of the six licences examined were hold by less than one third of these children. The licence to go to other places than school is the most frequently mentioned by the 7-to-11-year olds. But only half of primary school children can benefit from such a basic and critical licence that can be seen as a prerequisite to the development of activities independently from adults in the dwelling area. For the other half of the primary school children, this result supports the idea that most out-of-school activities are likely to take place in adult-controlled settings, where children must be accompanied by their parents. Both primary and secondary school children claim to have a particular licence more often than parents declare to grant their children that licence. The differences between children's and parents' responses are particularly obvious as regard to the licence to ride on main roads and, to a lesser degree, the licence to go out after dark. A cluster analysis permitted to isolate five contrasted types of independent mobility defined by various combinations of licences ranging from a quasi-total dependent mobility to the largest independent mobility. Age is the principal factor significantly associated with each of the five clusters, whereas gender is only associated to one cluster. The type of area and the children's perception of safety in their local area also seem to account for the nature and degree of independent mobility. Overall, these results support the view that a complex array of factors intervenes in the development of children independent mobility, including environmental attributes of the living context such as city size, density and outdoor urban facilities

Multifunctional Glycoconjugates for Recruiting Natural Antibodies against Cancer Cells

Invited for the cover of this issue is Olivier Renaudet and co-workers at the Université Grenoble Alpes and funded by the European Research Council (CoG “LEGO′” no. 647938). The image illustrates a synthetic chemist playing with supramolecular structures to kill cancer cells by using natural antibodies present in the blood stream. Read the full text of the article at 10.1002/chem.201903327

Conception, synthèse et étude de modules de reconnaissance multivalents pour des anticorps

Despite significant progress in anti-cancer therapy, current treatments are still controversial due to numerous side effects. Targeted immunotherapy recently emerged as an ideal alternative to improve treatment modalities for cancer patients. However, very limited approaches are available today and major issues remain to be addressed. In this context, we are interested in the design of biomolecular structures, innovative and bifunctional, able to hijack endogenous antibodies - which are naturally present in the human blood stream - toward cancer cells without pre-immunisation. Since natural circulating antibodies are polyspecific and have the ability to interact with multiple carbohydrate antigens, we focused on the design of multivalent glycodendrimers, as ligands for endogenous antibodies. The first part of our study consisted in synthesizing several multivalent glycoconjugates, based on peptide scaffolds and obtained by chemoselective ligations. To evaluate their influence on antibodies, the nature of both the carbohydrate and the scaffold, and the valency were varied. Then, in a second part of the study, microarray assays were developed with a model lectin, the Helix Pomatia Agglutinin (HPA). Experimental procedures were designed to determine surface dissociation constant and IC50 values, leading to the identification of high affinity ligands for HPA in the nanomolar range. Microarray assays were confirmed by other analytical methods (BLI, ELLA). Finally, the assays on slides were adapted to human sera screening, in order to identify tridimensional architectures highly affine to sera antibodies. A large panel of glycoconjugates were screened by microarray with around twenty sera, leading to the determination of promising glycosylated structures, as antibody ligands. The latter could be subsequently used for our anti-cancer approach.En dépit d’importants progrès dans le domaine de la thérapie anti-cancéreuse, les traitements actuels restent controversés, notamment en raison de la quantité importante d'effets secondaires induits. L'immunothérapie ciblée a récemment émergée en tant qu'alternative, afin d'améliorer les modalités de traitement des patients atteints du cancer. Malgré tout, seul un nombre limité d’approches sont aujourd’hui disponibles, et une grande partie des problèmes demeurent actuellement sans solution. C'est dans ce contexte que nous nous sommes intéressés à la conception de structures biomoléculaires innovantes et bifonctionnelles, capables de rediriger des anticorps endogènes, présents naturellement dans la circulation sanguine de l'homme, contre les tumeurs et, ce, sans immunisation préalable. Les anticorps naturels circulant étant polyspécifiques et ayant la capacité d’interagir avec des antigènes glycosylés, nous nous sommes plus particulièrement concentrés sur la conception de glycoconjugués multivalents, ligands d’anticorps endogènes. Une première partie de notre étude a consisté à synthétiser différents glycodendrimères multivalents, reposant sur des châssis peptidiques et obtenus par ligations chimiosélectives, tout en variant la nature du motif glycosylé et des plateformes, ainsi que la valence du conjugué. Puis, dans un second temps, des tests d’interaction par biopuce ont été mis en place avec une lectine modèle, la lectine Helix Pomatia Agglutinin (HPA). Des protocoles expérimentaux visant à calculer des constantes de dissociation de surface, ainsi que des IC50 ont été mis en place, permettant d’identifier de bons ligands de HPA avec des affinités de l’ordre du nanomolaire. Les tests par biopuce ont ensuite été confirmés avec d’autres méthodes d’analyses (BLI, ELLA). Finalement, afin d'identifier des architectures tri-dimensionnelles permettant une affinité optimale avec des anticorps, les tests d’interaction ont été adaptés au criblage de séra humains. Un large panel de glycoconjugués a alors été criblé par biopuce avec une vingtaine de séra, permettant la détermination de structures glycosylés prometteuses, qui pourront par la suite être utilisées dans le cadre de notre approche anti-cancéreuse

Design, synthesis and study of multivalent antibody binding modules

En dépit d’importants progrès dans le domaine de la thérapie anti-cancéreuse, les traitements actuels restent controversés, notamment en raison de la quantité importante d'effets secondaires induits. L'immunothérapie ciblée a récemment émergée en tant qu'alternative, afin d'améliorer les modalités de traitement des patients atteints du cancer. Malgré tout, seul un nombre limité d’approches sont aujourd’hui disponibles, et une grande partie des problèmes demeurent actuellement sans solution. C'est dans ce contexte que nous nous sommes intéressés à la conception de structures biomoléculaires innovantes et bifonctionnelles, capables de rediriger des anticorps endogènes, présents naturellement dans la circulation sanguine de l'homme, contre les tumeurs et, ce, sans immunisation préalable. Les anticorps naturels circulant étant polyspécifiques et ayant la capacité d’interagir avec des antigènes glycosylés, nous nous sommes plus particulièrement concentrés sur la conception de glycoconjugués multivalents, ligands d’anticorps endogènes. Une première partie de notre étude a consisté à synthétiser différents glycodendrimères multivalents, reposant sur des châssis peptidiques et obtenus par ligations chimiosélectives, tout en variant la nature du motif glycosylé et des plateformes, ainsi que la valence du conjugué. Puis, dans un second temps, des tests d’interaction par biopuce ont été mis en place avec une lectine modèle, la lectine Helix Pomatia Agglutinin (HPA). Des protocoles expérimentaux visant à calculer des constantes de dissociation de surface, ainsi que des IC50 ont été mis en place, permettant d’identifier de bons ligands de HPA avec des affinités de l’ordre du nanomolaire. Les tests par biopuce ont ensuite été confirmés avec d’autres méthodes d’analyses (BLI, ELLA). Finalement, afin d'identifier des architectures tri-dimensionnelles permettant une affinité optimale avec des anticorps, les tests d’interaction ont été adaptés au criblage de séra humains. Un large panel de glycoconjugués a alors été criblé par biopuce avec une vingtaine de séra, permettant la détermination de structures glycosylés prometteuses, qui pourront par la suite être utilisées dans le cadre de notre approche anti-cancéreuse.Despite significant progress in anti-cancer therapy, current treatments are still controversial due to numerous side effects. Targeted immunotherapy recently emerged as an ideal alternative to improve treatment modalities for cancer patients. However, very limited approaches are available today and major issues remain to be addressed. In this context, we are interested in the design of biomolecular structures, innovative and bifunctional, able to hijack endogenous antibodies - which are naturally present in the human blood stream - toward cancer cells without pre-immunisation. Since natural circulating antibodies are polyspecific and have the ability to interact with multiple carbohydrate antigens, we focused on the design of multivalent glycodendrimers, as ligands for endogenous antibodies. The first part of our study consisted in synthesizing several multivalent glycoconjugates, based on peptide scaffolds and obtained by chemoselective ligations. To evaluate their influence on antibodies, the nature of both the carbohydrate and the scaffold, and the valency were varied. Then, in a second part of the study, microarray assays were developed with a model lectin, the Helix Pomatia Agglutinin (HPA). Experimental procedures were designed to determine surface dissociation constant and IC50 values, leading to the identification of high affinity ligands for HPA in the nanomolar range. Microarray assays were confirmed by other analytical methods (BLI, ELLA). Finally, the assays on slides were adapted to human sera screening, in order to identify tridimensional architectures highly affine to sera antibodies. A large panel of glycoconjugates were screened by microarray with around twenty sera, leading to the determination of promising glycosylated structures, as antibody ligands. The latter could be subsequently used for our anti-cancer approach

Evolution des principaux rejets industriels entre 1986 et 1994

Available from INIST (FR), Document Supply Service, under shelf-number : RP 185 (4180) / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEMinistere de l'Environnement, 75 - Paris (France). Service de l'Environnement Industriel (SEI)FRFranc

Evolution des principaux rejets industriels entre 1986 et 1994

Available from INIST (FR), Document Supply Service, under shelf-number : RP 185 (4180) / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEMinistere de l'Environnement, 75 - Paris (France). Service de l'Environnement Industriel (SEI)FRFranc