On the origin of the Norwegian lemming.

The Pleistocene glacial cycles resulted in significant changes in species distributions, and it has been discussed whether this caused increased rates of population divergence and speciation. One species that is likely to have evolved during the Pleistocene is the Norwegian lemming (Lemmus lemmus). However, the origin of this species, both in terms of when and from what ancestral taxon it evolved, has been difficult to ascertain. Here, we use ancient DNA recovered from lemming remains from a series of Late Pleistocene and Holocene sites to explore the species' evolutionary history. The results revealed considerable genetic differentiation between glacial and contemporary samples. Moreover, the analyses provided strong support for a divergence time prior to the Last Glacial Maximum (LGM), therefore likely ruling out a postglacial colonization of Scandinavia. Consequently, it appears that the Norwegian lemming evolved from a small population that survived the LGM in an ice-free Scandinavian refugium

A Fluorescent Glycolipid-Binding Peptide Probe Traces Cholesterol Dependent Microdomain-Derived Trafficking Pathways

10.1371/journal.pone.0002933PLoS ONE38

Interacting effects of change in climate, human population, land use, and water use on biodiversity and ecosystem services

Human population growth and resource use, mediated by changes in climate, land use, and water use, increasingly impact biodiversity and ecosystem services provision. However, impacts of these drivers on biodiversity and ecosystem services are rarely analyzed simultaneously and remain largely unknown. An emerging question is how science can improve the understanding of change in biodiversity and ecosystem service delivery and of potential feedback mechanisms of adaptive governance. We analyzed past and future change in drivers in south-central Sweden. We used the analysis to identify main research challenges and outline important research tasks. Since the 19th century, our study area has experienced substantial and interlinked changes; a 1.6°C temperature increase, rapid population growth, urbanization, and massive changes in land use and water use. Considerable future changes are also projected until the mid-21st century. However, little is known about the impacts on biodiversity and ecosystem services so far, and this in turn hampers future projections of such effects. Therefore, we urge scientists to explore interdisciplinary approaches designed to investigate change in multiple drivers, underlying mechanisms, and interactions over time, including assessment and analysis of matching-scale data from several disciplines. Such a perspective is needed for science to contribute to adaptive governance by constantly improving the understanding of linked change complexities and their impacts

A new free-floating planet in the Upper Scorpius association

We report on a deep photometric survey covering an area of 1.17 deg$^2$ in the young Upper Scorpius stellar association using VIMOS $Iz$ and UKIDSS $ZJHK$ data taking several years apart. The search for the least massive population of Upper Scorpius ($\sim$5-10 Myr, 145 pc) is performed on the basis of various optical and infrared color-color and color-magnitude diagrams, including WISE photometry, in the magnitude interval $J$=14.5-19 mag (completeness), which corresponds to substellar masses from 0.028 through 0.004 M$_\odot$ at the age and distance of Upper Scorpius. We also present the proper motion analysis of the photometric candidates, finding that two objects successfully pass all photometric and astrometric criteria for membership in the young stellar association. One of them, UScoJ155150.2$-$213457, is a new discovery. We obtained low resolution, near-infrared spectroscopy ($R\sim$450, 0.85--2.35 $\mu$m) of this new finding using the FIRE instrument. We confirmed its low-gravity atmosphere expected for an Upper Scorpius member (weak alkaline lines, strong VO absorption, peaked $H$-band pseudocontinuum). By comparison with spectroscopic standards, we derive a spectral type of L6$\pm$1, and estimate a mass of $\approx$0.008-0.010 M$_\odot$ for UScoJ155150.2$-$213457. The colors and spectral slope of this object resemble those of other young, cool members of Upper Scorpius and $\sigma$ Orionis ($\sim$3 Myr) and field, high gravity dwarfs of related classification in contrast with the very red indices of field, low gravity, L-type dwarfs of intermediate age. UScoJ155150.2$-$213457, which does not show infrared flux excesses up to 4.5 $\mu$m, becomes one of the least massive and latest type objects known in the entire Upper Scorpius stellar association.Comment: 12 pages, 8 figures. Accepted for publication in A&

Rifampicin-Independent Interactions between the Pregnane X Receptor Ligand Binding Domain and Peptide Fragments of Coactivator and Corepressor Proteins

[Image: see text] The pregnane X receptor (PXR), a member of the nuclear receptor superfamily, regulates the expression of drug-metabolizing enzymes in a ligand-dependent manner. The conventional view of nuclear receptor action is that ligand binding enhances the receptor’s affinity for coactivator proteins, while decreasing its affinity for corepressors. To date, however, no known rigorous biophysical studies have been conducted to investigate the interaction among PXR, its coregulators, and ligands. In this work, steady-state total internal reflection fluorescence microscopy (TIRFM) and total internal reflection with fluorescence recovery after photobleaching were used to measure the thermodynamics and kinetics of the interaction between the PXR ligand binding domain and a peptide fragment of the steroid receptor coactivator-1 (SRC-1) in the presence and absence of the established PXR agonist, rifampicin. Equilibrium dissociation and dissociation rate constants of ~5 μM and ~2 s(−1), respectively, were obtained in the presence and absence of rifampicin, indicating that the ligand does not enhance the affinity of the PXR and SRC-1 fragments. Additionally, TIRFM was used to examine the interaction between PXR and a peptide fragment of the corepressor protein, the silencing mediator for retinoid and thyroid receptors (SMRT). An equilibrium dissociation constant of ~70 μM was obtained for SMRT in the presence and absence of rifampicin. These results strongly suggest that the mechanism of ligand-dependent activation in PXR differs significantly from that seen in many other nuclear receptors