Association between thermal responses, medical events, performance, heat acclimation and health status in male and female elite athletes during the 2019 Doha World Athletics Championships

Please read abstract in the article.The World Athletics Health and Science Department.http://bjsm.bmj.comhj2022Sports Medicin

the effect of heat therapy on neuromuscular function in humans

Cette thèse étudie l'effet de l’exposition répétée à la chaleur sur les adaptations musculaires chezl'homme. Deux protocoles ont été développés : un pour les sujets actifs et un pour lesimmobilisés. Les résultats montrent que la chaleur localisée n'a pas d'effet significatif sur lesadaptations musculaires chez les humains actifs. Cependant, la thérapie thermique corporelle apu réduire l'atrophie musculaire et maintenir la force musculaire lorsqu'elle est appliquée durantl'immobilisation et la rééducation.This thesis investigated the effects of repeated heat exposures on muscle adaptations in humansthrough two studies involving active and immobilized participants. The first study found thatlocalized heating did not affect muscle adaptations in active individuals. The second study showedthat whole-body heat therapy may reduce skeletal muscle atrophy and maintain muscle strengthduring immobilization and rehabilitation. In conclusion, repeated heat exposures may not benefitactive populations but could help prevent muscle deconditioning during immobilization andrehabilitation

Long Term Effects of Tillage–Crop Rotation Interaction on Soil Organic Carbon Pools and Microbial Activity on Wheat-Based System in Mediterranean Semi-Arid Region

Conservation agriculture based on no-tillage (NT) and crop rotation allows to enhance soil health. Based on data collected from long-term trials in a semi-arid region of Tunisia, results showed that NT increased significantly soil organic carbon stock (SOCS), soil microbial biomass carbon (SMBC), arbuscular mycorrhizal fungal (AMF) root colonization, and soil microbial respiration (CO2) at 0–20 cm topsoil layer compared to conventional tillage (CT). Moreover, triennial rotation (TRI), based on annual succession of Faba bean-Durum wheat-Barley, and biennial rotation (BI), based on annual succession of Faba bean-Durum wheat, increased significatively SMBC, AMF, and CO2. Likewise, a significant benefit of the two-way interactions Tillage × Rotation was observed. Furthermore, NT combined with TRI recorded the highest SOCS (2181 g C m−2), SMBC (515 mg C kg−1 soil), AMF (14%), and CO2 which is an indicator of soil microbial respiration (1071 mg CO2 kg−1 soil). The current results highlight the benefit adoption of minimum or (NT)combined with crop diversification on soil health

Long Term Effects of Tillage–Crop Rotation Interaction on Soil Organic Carbon Pools and Microbial Activity on Wheat-Based System in Mediterranean Semi-Arid Region

Conservation agriculture based on no-tillage (NT) and crop rotation allows to enhance soil health. Based on data collected from long-term trials in a semi-arid region of Tunisia, results showed that NT increased significantly soil organic carbon stock (SOCS), soil microbial biomass carbon (SMBC), arbuscular mycorrhizal fungal (AMF) root colonization, and soil microbial respiration (CO2) at 0–20 cm topsoil layer compared to conventional tillage (CT). Moreover, triennial rotation (TRI), based on annual succession of Faba bean-Durum wheat-Barley, and biennial rotation (BI), based on annual succession of Faba bean-Durum wheat, increased significatively SMBC, AMF, and CO2. Likewise, a significant benefit of the two-way interactions Tillage × Rotation was observed. Furthermore, NT combined with TRI recorded the highest SOCS (2181 g C m−2), SMBC (515 mg C kg−1 soil), AMF (14%), and CO2 which is an indicator of soil microbial respiration (1071 mg CO2 kg−1 soil). The current results highlight the benefit adoption of minimum or (NT)combined with crop diversification on soil health

Family specific genetic predisposition to breast cancer: results from Tunisian whole exome sequenced breast cancer cases

Abstract Background A family history of breast cancer has long been thought to indicate the presence of inherited genetic events that predispose to this disease. In North Africa, many specific epidemio-genetic characteristics have been observed in breast cancer families when compared to Western populations. Despite these specificities, the majority of breast cancer genetics studies performed in North Africa remain restricted to the investigation of the BRCA1 and BRCA2 genes. Thus, comprehensive data at a whole exome or whole genome level from local patients are lacking. Methods A whole exome sequencing (WES) of seven breast cancer Tunisian families have been performed using a family-based approach. We focused our analysis on BC-TN-F001 family that included two affected members that have been sequenced using WES. Relevant variants identified in BC-TN-F001 have been confirmed using Sanger sequencing. Then, we conducted an integrative analysis by combining our results with those from other WES studies in order to figure out the genetic transmission model of the newly identified genes. Biological network construction and protein–protein interactions analyses have been performed to decipher the molecular mechanisms likely accounting for the role of these genes in breast cancer risk. Results Sequencing, filtering strategies, and validation analysis have been achieved. For BC-TN-F001, no deleterious mutations have been identified on known breast cancer genes. However, 373 heterozygous, exonic and rare variants have been identified on other candidate genes. After applying several filters, 12 relevant high-risk variants have been selected. Our results showed that these variants seem to be inherited in a family specific model. This hypothesis has been confirmed following a thorough analysis of the reported WES studies. Enriched biological process and protein–protein interaction networks resulted in the identification of four novel breast cancer candidate genes namely MMS19, DNAH3, POLK and KATB6. Conclusions In this first WES application on Tunisian breast cancer patients, we highlighted the impact of next generation sequencing technologies in the identification of novel breast cancer candidate genes which may bring new insights into the biological mechanisms of breast carcinogenesis. Our findings showed that the breast cancer predisposition in non-BRCA families may be ethnic and/or family specific

Family specific genetic predisposition to breast cancer: results from Tunisian whole exome sequenced breast cancer cases.

International audienceBACKGROUND:A family history of breast cancer has long been thought to indicate the presence of inherited genetic events that predispose to this disease. In North Africa, many specific epidemio-genetic characteristics have been observed in breast cancer families when compared to Western populations. Despite these specificities, the majority of breast cancer genetics studies performed in North Africa remain restricted to the investigation of the BRCA1 and BRCA2 genes. Thus, comprehensive data at a whole exome or whole genome level from local patients are lacking.METHODS:A whole exome sequencing (WES) of seven breast cancer Tunisian families have been performed using a family-based approach. We focused our analysis on BC-TN-F001 family that included two affected members that have been sequenced using WES. Relevant variants identified in BC-TN-F001 have been confirmed using Sanger sequencing. Then, we conducted an integrative analysis by combining our results with those from other WES studies in order to figure out the genetic transmission model of the newly identified genes. Biological network construction and protein-protein interactions analyses have been performed to decipher the molecular mechanisms likely accounting for the role of these genes in breast cancer risk.RESULTS:Sequencing, filtering strategies, and validation analysis have been achieved. For BC-TN-F001, no deleterious mutations have been identified on known breast cancer genes. However, 373 heterozygous, exonic and rare variants have been identified on other candidate genes. After applying several filters, 12 relevant high-risk variants have been selected. Our results showed that these variants seem to be inherited in a family specific model. This hypothesis has been confirmed following a thorough analysis of the reported WES studies. Enriched biological process and protein-protein interaction networks resulted in the identification of four novel breast cancer candidate genes namely MMS19, DNAH3, POLK and KATB6.CONCLUSIONS:In this first WES application on Tunisian breast cancer patients, we highlighted the impact of next generation sequencing technologies in the identification of novel breast cancer candidate genes which may bring new insights into the biological mechanisms of breast carcinogenesis. Our findings showed that the breast cancer predisposition in non-BRCA families may be ethnic and/or family specific

Thermoregulatory responses during road races in hot-humid conditions at the 2019 Athletics World Championships

International audienceThis paper provides a kinetic observation of both core and skin temperatures in 108 elite athletes, during various outdoor competition events, adding to the very limited data so far available in the literature taken during elite competitions. The field skin temperature findings contrast previous laboratory findings, likely due to differences in relative air velocity and its impact on the evaporation of sweat. The rapid rise in skin temperature following cessation of exercise highlights the importance of infrared thermography measurements being taken during motion, not during breaks, when being used as a measurement of skin temperature during exercise

Association between thermal responses, medical events, performance, heat acclimation and health status in male and female elite athletes during the 2019 Doha World Athletics Championships

International audiencePurpose To determine associations between thermal responses, medical events, performance, heat acclimation and health status during a World Athletics Championships in hot-humid conditions. Methods From 305 marathon and race-walk starters, 83 completed a preparticipation questionnaire on health and acclimation. Core (T core ; ingestible pill) and skin (T skin ; thermal camera) temperatures were measured in-competition in 56 and 107 athletes, respectively. 70 in-race medical events were analysed retrospectively. Performance (% personal best) and did not finish (DNF) were extracted from official results. Results Peak T core during competition reached 39.6°C±0.6°C (maximum 41.1°C). T skin decreased from 32.2°C±1.3°C to 31.0°C±1.4°C during the races (p<0.001). T core was not related to DNF (25% of starters) or medical events (p≥0.150), whereas T skin , T skin rate of decrease and T core -to-T skin gradient were (p≤0.029). A third of the athletes reported symptoms in the 10 days preceding the event, mainly insomnia, diarrhoea and stomach pain, with diarrhoea (9% of athletes) increasing the risk of in-race medical events (71% vs 17%, p<0.001). Athletes (63%) who performed 5–30 days heat acclimation before the competition: ranked better (18±13 vs 28±13, p=0.009), displayed a lower peak T core (39.4°C±0.4°C vs 39.8°C±0.7°C, p=0.044) and larger in-race decrease in T skin (−1.4°C±1.0°C vs −0.9°C±1.2°C, p=0.060), than non-acclimated athletes. Although not significant, they also showed lower DNF (19% vs 30%, p=0.273) and medical events (19% vs 32%, p=0.179). Conclusion T skin , T skin rate of decrease and T core -to-T skin gradient were important indicators of heat tolerance. While heat-acclimated athletes ranked better, recent diarrhoea represented a significant risk factor for DNF and in-race medical events

Hydration and cooling in elite athletes: relationship with performance, body mass loss and body temperatures during the Doha 2019 IAAF World Athletics Championships

Purpose. To characterise hydration, cooling, body-mass loss, and core (Tcore) and skin (Tsk) temperatures during World Athletics Championships in hot-humid conditions. Methods. Marathon and race-walk (20km and 50km) athletes (n=83, 36 women) completed a pre-race questionnaire. Pre- and post-race body weight (n=74), Tcore (n= 56) and Tsk (n= 49; thermography) were measured. Results. Most athletes (93%) had a pre-planned drinking strategy [electrolytes (83%), carbohydrates (81%)] whilst ice-slurry was less common (11%; pConclusion. Most athletes hydration strategies were pre-planned based on personal experience. Ice-vests were the most adopted pre-cooling and the only one minimizing Tcore, suggesting that event organisers should be cognisant of logistic (i.e. freezers). Dehydration was moderate and unrelated to performance. Pre-race Tsk was related to performance and DNF, suggesting that Tsk modulation should be incorporated into pre-race strategies</div

Thermoregulatory responses during road races in hot-humid conditions at the 2019 Athletics World Championships

Purpose: To characterise thermoregulatory and performance responses of elite road-race athletes, while competing in hot, humid, night-time conditions during the 2019 IAAF World Athletic Championships.  Method: Male and female athletes, competing in the 20 km racewalk (n=20 males, 24 females), 50 km racewalk (n=19 males, 8 females) and marathon (n=15 males, 22 females) participated. Exposed mean skin (Tsk) and continuous core body (Tc) temperature were recorded with infrared thermography and ingestible telemetry pill, respectively.  Results: The range of ambient conditions (recorded roadside) were 29.3-32.7°C air temperature, 46-81 % relative humidity, 0.1-1.7 m∙s-1 air velocity and 23.5-30.6°C wet bulb globe temperature. Tc increased by 1.5 ± 0.1°C but mean Tsk decreased by 1.5 ± 0.4°C over the duration of the races. Tsk and Tc changed most rapidly at the start of the races and then plateaued, with Tc showing a rapid increase again at the end, in a pattern mirroring pacing. Performance times were between 3 to 20 % (mean = 113 ± 6%) longer during the championships compared to the personal best (PB) of athletes. Overall mean performance relative to PB was correlated with the wet-bulb globe temperature (WBGT) of each race (R2 = 0.89), but not with thermophysiological variables (R2 ≤ 0.3).  Conclusion: As previously reported in exercise heat stress, in this field study Tc rose with exercise duration, whereas Tsk showed a decline. The latter contradicts the commonly recorded rise and plateau in laboratory studies at similar ambient temperatures, but without realistic air movement.</p