1,002 research outputs found
WetNet operations
WetNet is an interdisciplinary Earth science data analysis and research project with an emphasis on the study of the global hydrological cycle. The project goals are to facilitate scientific discussion, collaboration, and interaction among a selected group of investigators by providing data access and data analysis software on a personal computer. The WetNet system fulfills some of the functionality of a prototype Product Generation System (PGS), Data Archive and Distribution System (DADS), and Information Management System for the Distributed Active Archive Center. The PGS functionality is satisfied in WetNet by processing the Special Sensor Microwave/Imager (SSM/I) data into a standard format (McIDAS) data sets and generating geophysical parameter Level II browse data sets. The DADS functionality is fulfilled when the data sets are archived on magneto optical cartridges and distributed to the WetNet investigators. The WetNet data sets on the magneto optical cartridges contain the complete WetNet processing, catalogue, and menu software in addition to SSM/I orbit data for the respective two week time period
Hi-C 3.0: Improved Protocol for Genome-Wide Chromosome Conformation Capture
The intricate folding of chromatin enables living organisms to store genomic material in an extremely small volume while facilitating proper cell function. Hi-C is a chromosome conformation capture (3C)-based technology to detect pair-wise chromatin interactions genome-wide, and has become a benchmark tool to study genome organization. In Hi-C, chromatin conformation is first captured by chemical cross-linking of cells. Cells are then lysed and subjected to restriction enzyme digestion, before the ends of the resulting fragments are marked with biotin. Fragments within close 3D proximity are ligated, and the biotin label is used to selectively enrich for ligated junctions. Finally, isolated ligation products are prepared for high-throughput sequencing, which enables the mapping of pair-wise chromatin interactions genome-wide. Over the past decade, next-generation sequencing has become cheaper and easier to perform, enabling more interactions to be sampled to obtain higher resolution in chromatin interaction maps. Here, we provide an in-depth guide to performing an up-to-date Hi-C procedure on mammalian cell lines. These protocols include recent improvements that increase the resolution potential of the assay, namely by enhancing cross-linking and using a restriction enzyme cocktail. These improvements result in a versatile Hi-C procedure that enables the detection of genome folding features at a wide range of distances. Basic Protocol 1: Fixation of nuclear conformation Basic Protocol 2: Chromosome conformation capture Basic Protocol 3: Hi-C sequencing library preparation
Vesignieite BaCu3V2O8(OH)2 as a Candidate Spin-1/2 Kagome Antiferromagnet
A polycrystalline sample of vesignieite BaCu3V2O8(OH)2 comprising a nearly
ideal kagome lattice composed of Cu2+ ions carrying spin 1/2 has been
synthesized and studied by magnetization and heat capacity measurements.
Magnetic susceptibility shows a neither long range order, a spin glass
transition nor a spin gap down to 2 K, in spite of a moderately strong
antiferromagnetic interaction of J/kB = 53 K between nearest-neighbor spins. A
broad peak observed at a temperature corresponding to 0.4J in intrinsic
magnetic susceptibility indicates a marked development of the short-range
order. The ground state of vesignieite is probably a gapless spin liquid or is
accompanied by a very small gap less than J/30.Comment: 4 pages, 5 figure
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Sensory Directed Mixture Study of Beers Dry-Hopped with Cascade, Centennial, and Chinook
American craft beer style and flavor is often driven by the unique qualities of American hops. Cascade, Chinook, and Centennial hops are used prominently for dry-hopping singly and/or in blends to impart an intense hoppy aroma to beer. A sensory directed dry-hopping mixture study was performed to understand the contribution that each of these hops make to beer aroma. Utilizing a 4th degree simplex-lattice mixture-design, sixteen beers were prepared (including an "unhopped" control) by dry-hopping a common "unhopped" base beer with different blends of ground whole cone hops made from the three hop cultivars. The treatments were evaluated by trained panelists using descriptive analysis, where the response variables used by the panel encompassed the sensory attributes that described the unique aromatic features of these three hops, (i.e., citrus, tropical/fruity, tropical/catty, and herbal). Using these outputs, the sensory contributions of each individual cultivar, as well as mixtures of the cultivars, were examined on a per attribute basis. These results can be used to select combinations or blends of the three hops for use during dry-hopping that provide similar or dissimilar overall aroma intensity and quality in dry-hopped beer
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Impact of static dry-hopping rate on the sensory and analytical profiles of beer
Dry-hopping is a technique that has been used by brewers to increase the hop aroma and flavour of beer for centuries. Throughout the twenty first century, dry-hopping has become an increasingly popular method among craft brewers to impart intense hoppy aroma and flavour to beer. Many US craft brewers use extremely high dry-hop dosing rates of up to 2200 g/hL and this is both unsustainable and potentially wasteful. This study examines the impact of dry-hopping rate on the sensorial and analytical characteristics of dry-hopped beers. An unhopped pale beer was statically dry-hopped with whole cone Cascade from the 2015 harvest over a broad range of dry-hopping rates (200-1600 g/hL) in replicated, pilot scale (80 L) aliquots. Trained panellists using descriptive analysis scaled the overall and qualitative hop aroma intensity of these beers, as well as the unhopped base beer. Instrumental analysis was used to measure the levels of hop volatile and non-volatile extraction between the treatments. The relationship between dry-hopping rate and the sensorial and analytical characteristics of the finished beer was not linear and, based on the extraction efficiencies of select hop volatiles, had an ideal range between 400 and 800 g/hL. (C) 2018 The Institute of Brewing & Distillin
Tres monteas escurialenses
[ES] Sin resumenLópez Mozo, A. (2008). Tres monteas escurialenses. EGA. Revista de Expresión Gráfica Arquitectónica. (13):190-197. https://doi.org/10.4995/ega.2008.102841901971
High-Field ESR Measurements of S=1/2 Kagome Lattice Antiferromagnet BaCuVO(OH)
High-field electron spin resonance (ESR) measurements have been performed on
vesignieite BaCuVO(OH), which is considered as a nearly ideal
model substance of =1/2 kagome antiferromagnet, in the temperature region
from 1.9 to 265 K. The frequency region is from 60 to 360 GHz and the applied
pulsed magnetic field is up to 16 T. Observed g-value and linewidth show the
increase below 20 K, which suggest the development of the short range order.
Moreover, a gapless spin liquid ground state is suggested from the
frequency-field relation at 1.9 K.Comment: 5 pages, 6 figures, jpsj2 class file, to be published in J. Phys.
Soc. Jp
Stress-driven instability in growing multilayer films
We investigate the stress-driven morphological instability of epitaxially
growing multilayer films, which are coherent and dislocation-free. We construct
a direct elastic analysis, from which we determine the elastic state of the
system recursively in terms of that of the old states of the buried layers. In
turn, we use the result for the elastic state to derive the morphological
evolution equation of surface profile to first order of perturbations, with the
solution explicitly expressed by the growth conditions and material parameters
of all the deposited layers. We apply these results to two kinds of multilayer
structures. One is the alternating tensile/compressive multilayer structure,
for which we determine the effective stability properties, including the effect
of varying surface mobility in different layers, its interplay with the global
misfit of the multilayer film, and the influence of asymmetric structure of
compressive and tensile layers on the system stability. The nature of the
asymmetry properties found in stability diagrams is in agreement with
experimental observations. The other multilayer structure that we study is one
composed of stacked strained/spacer layers. We also calculate the kinetic
critical thickness for the onset of morphological instability and obtain its
reduction and saturation as number of deposited layers increases, which is
consistent with recent experimental results. Compared to the single-layer film
growth, the behavior of kinetic critical thickness shows deviations for upper
strained layers.Comment: 27 pages, 11 figures; Phys. Rev. B, in pres
Vertical integration and firm boundaries : the evidence
Since Ronald H. Coase's (1937) seminal paper, a rich set of theories has been developed that deal with firm boundaries in vertical or input–output structures. In the last twenty-five years, empirical evidence that can shed light on those theories also has been accumulating. We review the findings of empirical studies that have addressed two main interrelated questions: First, what types of transactions are best brought within the firm and, second, what are the consequences of vertical integration decisions for economic outcomes such as prices, quantities, investment, and profits. Throughout, we highlight areas of potential cross-fertilization and promising areas for future work
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