PHYSIOLOGICAL RESPONSES OF HOLSTEIN COW’S FACE TO RESOURCE OF FAN IN FREE-STALL

Este estudo teve como objetivo avaliar a influência do recurso de ventilação sobre a fisiologia de vacas Holandesas alojadas em free-stall, durante o verão do sudeste brasileiro. Foram utilizadas 20 vacas Holandesas submetidas a dois tratamentos: controle e ventilação. Os parâmetros ambientais registrados foram temperatura e umidade relativa do ar e temperatura de globo negro. As variáveis fisiológicas avaliadas foram temperatura retal e frequência respiratória. Para obtenção das estatísticas descritivas utilizou-se a metodologia de quadrados mínimos. Apesar das diferenças estatísticas obtidas para as variáveis fisiológicas, as mesmas não foram biologicamente efetivas e indicaram que os animais se encontravam em conforto térmico

Transcripts levels of enzymes involved in histone acetylation in bovine oocytes of different competences.

Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstract

Voluntary Intake of Tanzania Grass (\u3ci\u3ePanicum maximum\u3c/i\u3e) under Rotational Grazing by Lactating Cows

The study was conducted at Nucleo de Pesquisas Zootecnicas Nordeste of the Instituto de Zootecnia, Ribeirão Preto, SP, in a rotational grazing area of Tanzania grass (Panicum maximum), to estimate the dry matter intake by lactanting cows. The estimation of dry matter intake was calculated from the feces production estimated using extrusa Chromium-mordent and the in vitro digestibility of diet. The three treatments were crossbreed cows fed 3 kg.day-1 of concentrate, crossbred cows without concentrate suplementation and pure Gir cows also without concentrate supplementation. The milk production was 11.98, 6.53 and 5.46 kg per cow per day, the grass intake was 8.26 ± 5.66, 11.01 ± 5.37 and 9.55 ± 2.31 kg of dry matter per day or 2.15%, 2.37% and 2.34% of live weight for the three experimental groups respectively. The milk production was higher (P\u3c 0.01) for cows fed with concentrate. No difference was found for dry matter intake

Influence of pre-slaughter management on cortisol level in lambs

This study aimed to verify the influence of the transport in open or closed compartments, followed by two resting periods (1 and 3 hours) for the slaughter process on the levels of cortisol as a indicative of stress level in lambs. The slaughterhouse was located 85 km away from the place of confinement and the transportation of the lambs was carried out in a cage truck type, each cage had a divider in the middle making the front without external visual access to the environment, and the back portion of the cages with visual access to the road. At the slaughterhouse, blood samples were taken from 86 lambs after the transport and before slaughter (1 or 3 hours of resting) for plasma cortisol analysis. Variables were evaluated through the PROC GLM procedure from the Statistical Analysis System ©, version 9.1.3 software.The method of transport influenced in the cortisol concentration (P<0.01), the animals transported in the closed compartment had a lower level. After the resting period in the slaughterhouse, there was a decline in the plasmatic cortisol concentration, with the animals subjected to three hours of rest presenting the lower average cortisol value (P<0.05). It can be inferred that the lambs that remained three hours in standby before slaughter had more time to recover from the stress of the transportation than those that waited just one hour. Visual access to the external environment during the transport of the lambs is a stressful factor changing the level of plasmatic cortisol, and the resting period before slaughter was effective in lowering stress, reducing the plasmatic cortisol in the lambs

Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle.

We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, residual feed intake (RFI), ultrasound backfat, rump fat, and ribeye area

The impact of the urban canyon geometry in the nocturnal heat island intensity: analysis by a simplified model adapted to a GIS

A geometria urbana é um dos fatores de maior influência na intensidade da ilha de calor urbana. Seu estudo requer a caracterização de cânions urbanos, geralmente medidos pela relação entre a altura dos edifícios e a largura da rua (H/W), conceito aplicado no modelo numérico de Oke em 1981. O objetivo deste artigo é verificar o impacto da geometria do cânion urbano na intensidade de ilhas de calor noturna. Para isso, foram realizados levantamento de dados climáticos e de geometria urbana em duas cidades brasileiras. Os valores de intensidade de ilha de calor foram confrontados com os simulados pelo modelo original de Oke (1981), o qual foi calibrado e adaptado à plataforma SIG, de forma a possibilitar a incorporação de outro parâmetro de geometria, além da relação H/W: o comprimento de rugosidade. Esse processo gerou uma nova ferramenta de cálculo, que é denominda THIS (Tool for Heat Island Simulation). Aplicou-se o novo modelo para simular alguns cenários urbanos hipotéticos, que representam vários tipos de cânions urbanos. Os resultados demonstraram que cânions urbanos de maior rugosidade amenizam as intensidades de ilha de calor noturna em relação a um cânion de mesmo valor de relação H/W e menor rugosidade.Urban geometry is one of the main factors influencing the development of urban heat islands. The study of urban geometry requires a characterization of urban canyons, which can be usually measured by the H/W ratio (a relationship between the height and the width of a street), a concept applied in a numerical model by Oke in 1981. The aim of this paper is to verify the impact of the canyon geometry on the intensity of the nocturnal urban heat islands. For this purpose, measurements of climate data and urban geometry were conducted in two Brazilian cities. The values of heat island intensity were cross-examined to those generated with the application of the original Oke's model. Therefore, this latter was calibrated and adapted to run in a GIS platform, allowing the incorporation of a geometric parameter other than the H/W ratio - the roughness length. Then, this process produced a new calculation tool, which is called THIS (Tool for Heat Island Simulation). The new model was applied to simulate some hypothetical urban scenarios representing several urban canyons types. The results showed that the urban canyons with the largest roughness reduce the nocturnal heat island intensities in relation to an urban canyon of the same H/W value, but presenting lower roughness rates instead.Fundação de Amparo à Pesquisa do Estado de São Paulo (Fapesp)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Melatonin reduces apoptotic cells, SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts.

Made available in DSpace on 2018-05-24T00:51:21Z (GMT). No. of bitstreams: 1 Marquesetal2018ReproductioninDomesticAnimals.pdf: 863164 bytes, checksum: a92b3abd11502c3e0ec94a3c3d43075c (MD5) Previous issue date: 2018-05-23bitstream/item/177557/1/Marques-et-al-2018-Reproduction-in-Domestic-Animals.pd

A Comparative Study of the Outer Membrane Proteome from an Atypical and a Typical Enteropathogenic Escherichia coli

This study compared the proteomic profile of outer membrane proteins (OMPs) from one strain of atypical enteropathogenic Escherichia coli (aEPEC) and one of typical EPEC (tEPEC). The OMPs fractions were obtained using sarcosine extraction, and analyzed by one- and two-dimensional gel electrophoresis (1DE and 2DE, respectively). The 1DE OMPs analysis of typical and atypical EPEC evidenced similar patterns; however, the 2DE OMP profile from the aEPEC revealed more protein spots in the 40- to 70-kDa region. 2DE image analysis identified 159 protein spots in both strains whereas 53 protein spots were observed only in tEPEC and 128 were observed only in aEPEC. Remarkably, 41.5% of aEPEC spots showed higher levels of expression compared to tEPEC, some of which with two, others four or even five times more. Twenty-four selected spots were identified using MALDI-TOF mass spectrometry and they corresponded to proteins involved in cell structure and metabolism, as well as in gene regulation. Some of these proteins showed similarity with proteins identified in other E. coli pathotypes. Besides, the differential expression of some proteins in aEPEC may suggest that it could be related to their features that ascertain the adaptation to distinct environments and the worldwide spread distribution of these pathogens

Isokinetic evaluation of knee muscles in soccer players: discriminant analysis [Avaliação isocinética dos músculos do joelho em jogadores de futebol: análise discriminante]; [Evaluación isocinética de los músculos de la rodilla en jugadores de fútbol: análisis discriminante]

Introduction: Muscle activity in soccer players can be measured by isokinetic dynamometer, which is a reliable tool for assessing human performance. Objectives: To perform isokinetic analyses and to determine which variables differentiate the under-17 (U17) soccer category from the professional (PRO). Methods: Thirty four players were assessed (n=17 for each category). The isokinetic variables used for the knee extension-flexion analysis were: peak torque (Nm), total work (J), average power (W), angle of peak torque (deg.), agonist/ antagonist ratio (%), measured for three velocities (60°/s, 120°/s and 300°/s), with each series containing five repetitions. Three Wilks' Lambda discriminant analyses were performed, to identify which variables were more significant for the definition of each of the categories. Results: The discriminative variables at 60°/s in the PRO category were: extension peak torque, flexion total work, extension average power and agonist/antagonist ratio; and for the U17s were: extension total work, flexion peak torque and flexion average power. At 120°/s for the PRO category the discriminant variables were: flexion peak torque and extension average power; for the U17s they were: extension total work and flexion average power. Finally at 300°/s, the variables found in the PRO and U17 categories respectively were: extension average power and extension total work. Conclusion: Isokinetic variables for flexion and extension knee muscles were able to significantly discriminate between PRO and U17 soccer players. RESUMO Introdução: A atividade muscular em jogadores de futebol pode ser medida por meio do dinamômetro isocinético, que é um instrumento confiável para avaliação do desempenho humano. Objetivos: Conduzir análises isocinéticas e discriminar quais variáveis diferenciam a categoria sub-17 (S17) da profissional (PRO). Métodos: Trinta e quatro jogadores de futebol (n=17 para cada categoria) foram avaliados. As variáveis isocinéticas utilizadas para a análise de extensão-flexão do joelho foram: pico de torque (Nm), trabalho total (J), potência média (W), ângulo de pico de torque (graus), razão agonista/antagonista (%), testadas em três velocidades (60°/s, 120°/s e 300°/s), com cada série contendo cinco repetições. Três análises discriminantes foram feitas usando o método Wilk's Lambda para identificar quais variáveis fariam uma discriminação significativa entre as duas categorias. Resultados: As variáveis discriminantes a 60°/s na categoria PRO foram: pico de torque extensores, trabalho total flexores, potência média de extensores e razão agonista/antagonista; e para os S17 foram: trabalho total de extensores, pico de torque de flexores e potência média de flexores. A 120°/s para a categoria PRO as variáveis discriminantes foram: pico de torque de flexores e potência média de extensores; para os S17 foram: trabalho total de extensores e potência média de flexores. A 300°/s, as variáveis encontradas para as categorias PRO e S17 foram, respectivamente: potência média de extensores e trabalho total de extensores. Conclusão: As variáveis isocinéticas para os músculos do joelho flexores e extensores foram capazes de fazer uma discriminação significativa entre jogadores de futebol PRO e S17. RESUMEN Introducción: La actividad muscular en jugadores de fútbol puede ser medida por medio del dinamómetro isocinético, que es un instrumento confiable para evaluación del desempeño humano. Objetivos: Conducir análisis isocinéticos y discriminar qué variables diferencian la categoría sub-17 (S17) de la profesional (PRO). Métodos: Fueron evaluados treinta y cuatro jugadores de fútbol (n=17 para cada categoría). Las variables isocinéticas utilizadas para el análisis de extensión-flexión de la rodilla fueron: pico de torque (Nm), trabajo total (J), potencia media (W), ángulo de pico de torque (grados), razón agonista/antagonista (%), probadas en tres velocidades (60°/s, 120°/s y 300°/s), con cada serie conteniendo cinco repeticiones. Fueron realizados tres análisis discriminantes usando el método Wilk's Lambda para identificar qué variables harían una discriminación significativa entre las dos categorías. Resultados: Las variables discriminantes a 60°/s en la categoría PRO fueron: pico de torque extensores, trabajo total flexores, potencia media de extensores y razón agonista/antagonista; y para los S17 fueron: trabajo total de extensores, pico de torque de flexores y potencia media de flexores. A 120°/s para la categoría PRO las variables discriminantes fueron: pico de torque de flexores y potencia media de extensores; para los S17 fueron: trabajo total de extensores y potencia media de flexores. A 300°/s, las variables encontradas para las categorías PRO y S17 fueron, respectivamente: potencia media de extensores y trabajo total de extensores. Conclusión: Las variables isocinéticas para los músculos de la rodilla flexores y extensores fueron capaces de hacer una discriminación significativa entre jugadores de fútbol PRO y S17