Globalization and the dynamics of international fish trade

Although the share of total fish supply which is traded internationally is fairly stable, the patterns of trade flows are in constant change. China and Viet Nam are increasingly emerging as processors and suppliers of value-added products to world markets, but also within the world’s various regions new players are emerging and markets developing. On the supply side, the aquaculture sector is becoming a major factor in product innovation and price determination with it share of total supply for human consumption quickly approaching 50%. In this paper, the authors presents an overview of world supply and trade flows with a particular emphasis on the impact of globalisation on developing country producers and exporters. Finally, a reference is made to the multilateral and bilateral trade system within which the fisheries sector is operating

Effects of Companion Animal Ownership among Canadian Street-involved Youth: A Qualitative Analysis

In Canada, approximately 150,000 youth are homeless on any given night, and many have companion animals. Through a series of semi-structured interviews, this qualitative study explored the issues and effects of companion animal ownership among street-involved youth from the perspective of the youth themselves. Pet before self was the substantive theme, with first level sub-themes of physical and emotional effects. Previously unidentified findings include benefits of having a companion animal, such as creating structure and routine and decreasing use of drugs. Loss of the companion animal was a negative effect. Youth consistently reported making choices to stay with their animal regardless of liabilities for their own health or success. Service providers should understand and support the significant human-animal bond that can exist for these homeless youth

Lentiviral gene transfer of RPE65 rescues survival and function of cones in a mouse model of Leber congenital amaurosis.

BACKGROUND: RPE65 is specifically expressed in the retinal pigment epithelium and is essential for the recycling of 11-cis-retinal, the chromophore of rod and cone opsins. In humans, mutations in RPE65 lead to Leber congenital amaurosis or early-onset retinal dystrophy, a severe form of retinitis pigmentosa. The proof of feasibility of gene therapy for RPE65 deficiency has already been established in a dog model of Leber congenital amaurosis, but rescue of the cone function, although crucial for human high-acuity vision, has never been strictly proven. In Rpe65 knockout mice, photoreceptors show a drastically reduced light sensitivity and are subject to degeneration, the cone photoreceptors being lost at early stages of the disease. In the present study, we address the question of whether application of a lentiviral vector expressing the Rpe65 mouse cDNA prevents cone degeneration and restores cone function in Rpe65 knockout mice. METHODS AND FINDINGS: Subretinal injection of the vector in Rpe65-deficient mice led to sustained expression of Rpe65 in the retinal pigment epithelium. Electroretinogram recordings showed that Rpe65 gene transfer restored retinal function to a near-normal pattern. We performed histological analyses using cone-specific markers and demonstrated that Rpe65 gene transfer completely prevented cone degeneration until at least four months, an age at which almost all cones have degenerated in the untreated Rpe65-deficient mouse. We established an algorithm that allows prediction of the cone-rescue area as a function of transgene expression, which should be a useful tool for future clinical trials. Finally, in mice deficient for both RPE65 and rod transducin, Rpe65 gene transfer restored cone function when applied at an early stage of the disease. CONCLUSIONS: By demonstrating that lentivirus-mediated Rpe65 gene transfer protects and restores the function of cones in the Rpe65(-/-) mouse, this study reinforces the therapeutic value of gene therapy for RPE65 deficiencies, suggests a cone-preserving treatment for the retina, and evaluates a potentially effective viral vector for this purpose

Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice

Dominant mutations in the rhodopsin gene, which is expressed in rod photoreceptor cells, are a major cause of the hereditary-blinding disease, autosomal dominant retinitis pigmentosa. Therapeutic strategies designed to edit such mutations will likely depend on the introduction of double-strand breaks and their subsequent repair by homologous recombination or non-homologous end joining. At present, the break repair capabilities of mature neurons, in general, and rod cells, in particular, are undefined. To detect break repair, we generated mice that carry a modified human rhodopsin-GFP fusion gene at the normal mouse rhodopsin locus. The rhodopsin-GFP gene carries tandem copies of exon 2, with an ISceI recognition site situated between them. An ISceI-induced break can be repaired either by non-homologous end joining or by recombination between the duplicated segments, generating a functional rhodopsin-GFP gene. We introduced breaks using recombinant adeno-associated virus to transduce the gene encoding ISceI nuclease. We found that virtually 100% of transduced rod cells were mutated at the ISceI site, with ∼85% of the genomes altered by end joining and ∼15% by the single-strand annealing pathway of homologous recombination. These studies establish that the genomes of terminally differentiated rod cells can be efficiently edited in living organisms

Socializing One Health: an innovative strategy to investigate social and behavioral risks of emerging viral threats

In an effort to strengthen global capacity to prevent, detect, and control infectious diseases in animals and people, the United States Agency for International Development’s (USAID) Emerging Pandemic Threats (EPT) PREDICT project funded development of regional, national, and local One Health capacities for early disease detection, rapid response, disease control, and risk reduction. From the outset, the EPT approach was inclusive of social science research methods designed to understand the contexts and behaviors of communities living and working at human-animal-environment interfaces considered high-risk for virus emergence. Using qualitative and quantitative approaches, PREDICT behavioral research aimed to identify and assess a range of socio-cultural behaviors that could be influential in zoonotic disease emergence, amplification, and transmission. This broad approach to behavioral risk characterization enabled us to identify and characterize human activities that could be linked to the transmission dynamics of new and emerging viruses. This paper provides a discussion of implementation of a social science approach within a zoonotic surveillance framework. We conducted in-depth ethnographic interviews and focus groups to better understand the individual- and community-level knowledge, attitudes, and practices that potentially put participants at risk for zoonotic disease transmission from the animals they live and work with, across 6 interface domains. When we asked highly-exposed individuals (ie. bushmeat hunters, wildlife or guano farmers) about the risk they perceived in their occupational activities, most did not perceive it to be risky, whether because it was normalized by years (or generations) of doing such an activity, or due to lack of information about potential risks. Integrating the social sciences allows investigations of the specific human activities that are hypothesized to drive disease emergence, amplification, and transmission, in order to better substantiate behavioral disease drivers, along with the social dimensions of infection and transmission dynamics. Understanding these dynamics is critical to achieving health security--the protection from threats to health-- which requires investments in both collective and individual health security. Involving behavioral sciences into zoonotic disease surveillance allowed us to push toward fuller community integration and engagement and toward dialogue and implementation of recommendations for disease prevention and improved health security

Ecological applications of near infrared reflectance spectroscopy - a tool for rapid, cost-effective prediction of the composition of plant and animal tissues and aspects of animal performance

Many ecological studies rely heavily on chemical analysis of plant and animal tissues. Often, there is limited time and money to perform all the required analyses and this can result in less than ideal sampling schemes and poor levels of replication. Near infrared reflectance spectroscopy (NIRS) can relieve these constraints because it can provide quick, non-destructive and quantitative analyses of an enormous range of organic constituents of plant and animal tissues. Near infrared spectra depend on the number and type of C H, N H and O H bonds in the material being analyzed. The spectral features are then combined with reliable compositional or functional analyses of the material in a predictive statistical model. This model is then used to predict the composition of new or unknown samples. NIRS can be used to analyze some specific elements (indirectly – e.g., N as protein) or well-defined compounds (e.g., starch) or more complex, poorly defined attributes of substances (e.g., fiber, animal food intake) have also been successfully modeled with NIRS technology. The accuracy and precision of the reference values for the calibration data set in part determines the quality of the predictions made by NIRS. However, NIRS analyses are often more precise than standard laboratory assays. The use of NIRS is not restricted to the simple determination of quantities of known compounds, but can also be used to discriminate between complex mixtures and to identify important compounds affecting attributes of interest. Near infrared reflectance spectroscopy is widely accepted for compositional and functional analyses in agriculture and manufacturing but its utility has not yet been recognized by the majority of ecologists conducting similar analyses. This paper aims to stimulate interest in NIRS and to illustrate some of the enormous variety of uses to which it can be put. We emphasize that care must be taken in the calibration stage to prevent propagation of poor analytical work through NIRS, but, used properly, NIRS offers ecologists enormous analytical power

The LV-RPE65 Vector Restores the ERG Response of the <i>Rpe65</i>^−/− Mice

<div><p><i>Rpe65</i>^−/− mice were treated P5 by an intravitreal injection of 20 ng of lentiviral vector.</p> <p>(A) ERG response to single flashes was recorded in scotopic condition at 2 mo of age. After LV-GFP treatment, the mice showed a response typical for <i>Rpe65</i>^−/− mice, with the a-wave absent and the b-wave elicited only for the highest stimuli (i.e., 10 and 25 cd s/m²; left tracings). After LV-RPE65 treatment, a stimulus of 10⁻² cd s/m² was sufficient to trigger the b-wave, and the a-wave appeared for a stimulus of 0.3 cd s/m² (right tracings). Vertical bars indicate occurrence of the stimuli; x-axis: 40 ms/div; y-axis: 100 μV/div.</p> <p>(B) Summary of the ERG recordings performed 2 mo after vector delivery at P5 in <i>Rpe65</i>^−/− mice. Amplitude of b-wave (expressed in μV) is represented for each stimulus intensity. In the LV-RPE65-treated group (squares), the b-wave rose with a stimulus as low as 10⁻² cd s/m², whereas in the LV-GFP-treated group (triangles) 3 cd s/m² were necessary to elicit the b-wave. ANOVA for repeated measures showed significant stimulus intensity effect (<i>p</i> < 0.0001), group effect (<i>p</i> = 0.023), and group versus stimulus intensity interaction (<i>p</i> = 0.002). Data are presented as mean ± standard error of the mean (SEM).</p> <p>(C) Improvement of the ERG response after LV-RPE65 treatment is correlated with the level of RPE65 expression. The area of the retina expressing GFP (triangles) or RPE65 (squares) was quantified for each eye at age 4 mo after gene transfer at P5, and plotted against the b-wave threshold (defined as the intensity of the lowest stimulus capable of eliciting the b-wave, expressed in log mcd s/m²).</p></div