Neuromuscular Responses during Knee Extension Exercise in Combination with Different Blood Flow Restriction Initial Pressures

Specifications of blood flow restriction training technique have been widely discussed to create a valid and reliable protocol. One of the unexplored variables is the effect of different initial restrictive pressures (tightness of cuffs, IRP) in combination with resistance exercise on neuromuscular responses and strength. PURPOSE: The purpose of this study was to determine any differences in amplitude (RMS) and median frequency (MDF) of electromyography (EMG) signals, as well as changes in strength during knee extension exercises with IRPs of 40-45 mmHg and 60-65 mmHg. METHODS: Twenty male subjects (age = 25.7 ± 4.3 yrs), participated in this study. They were required to attend the lab on 3 different occasions, with the first one being a familiarization session. On the subsequent sessions, participant\u27s upper leg was measured starting from the lateral epicondyle to the greater trochanter of the femur. An EMG electrode was placed at one-third the distance over the longitudinal axis of the vastus lateralis (VL) after shaving, abrading and cleaning with isopropyl alcohol. Initial restrictive pressure was randomly selected and participants completed a pre-exercise maximum voluntary contraction (MVC) test to determine their torque. For dynamic exercises, load was set at 20% MVC and each participant performed one set of 30 repetitions and three sets of 15 repetitions, separated by one minute rest. Post-exercise MVC was performed to assess the changes in leg strength following exercises. RESULTS: Results showed significant main effects (p\u3c0.01) in leg strength for condition (40-45 mmHg vs. 60-65 mmHg) and trial (pre vs. post MVC). A significant main effect was observed for condition for MVC EMG amplitude (p\u3c0.01). In addition, there were significant main effects for contractions (p\u3c0.01) in both EMG amplitude and MDF during dynamic exercises. CONCLUSION: The significant decreases in knee extension strength at IRP of 60-65 mmHg compared to IRP of 40-45mmHg from pre- to post-exercise suggest that subjects experienced more fatigue at 60-65 mmHg because of the decreased availability of oxygen to the working muscle. The use of different IRP will affect the level of blood flow and oxygen supply to skeletal muscle possibly causing variation in neuromuscular adaptation due to changes in total and type of muscle fiber recruitment

A Comparison of Hypertrophy Potential between the Vastus Lateralis and Rectus Femoris during Level Walking in Combination with Vascular Restriction.

Recent research has found that by compressing blood flow to working muscle during lower body exercise involving short bouts of walk training results in an increased muscle volume and improved quadriceps strength. However, the effects of vascular restriction (VR) during walking on neuromuscular adaptations as assessed by electromyography (EMG) activity may provide insight on how low intensity training with vascular restriction can be applied to various modes of exercise. PURPOSE: The purpose of this study was to explore and compare muscle activation changes of the quadriceps muscles during walking using EMG with and without blood flow restriction. METHODS: EMG electrodes were placed along the longitudinal axis of the vastus lateralis (VL) and rectus femoris (RF) of the right thigh of seven male volunteers (mean ± SD age = 22.7 ± 6.8 years; height = 178 ±5.4 cm; weight = 82.6 ± 7.7 kg). Placement of the electrode was marked at a point of 33.3% on VL, and RF 50% of the distance between the lateral femoral epicondyle and the greater trochanter. On two separate testing sessions, subjects walked on a treadmill for two, 10 minutes bouts separated by a 5 min rest period at a speed of 80.4 m*min-1 with and without blood flow restriction. RESULTS: There were significant main effects for both condition (with and without VR) and muscles (VL and RF), as well as an interaction between condition and muscles (condition*muscle) for EMG amplitude (RMS). On the other hand, there were no significant main effects or interactions for EMG median frequency (MDF). CONCLUSION: The findings suggest that walking with VR might cause neural adaptation in both VL and RF, but the VL might have a better potential for hypertrophic response when performing slow walking with blood flow restriction. Walking speed and the pressure used for compression during walking might change the pattern of response, therefore, both need to be given careful consideration in the design of future studies

Neuromuscular Responses of the Vastus Lateralis to Slow Walking With Vascular Restriction.

Motor unit recruitment of individual muscles could substantially differ with changes in speed and load. However, the recruitment patterns associated with low intensity walk training with vascular restriction at sustained loads and constant speed could be different than normal walking. Vascular restricted (VR) walk training is an effective method for increasing muscular strength; however the changes in motor unit recruitment and firing frequency have not been investigated to understand the mechanisms underlying the neuromuscular adaptations. Purpose: The purpose of this study was to analyze differences in electrical activity of the vastus lateralis during VR and non vascular restricted (non-VR) walking sessions. Methods: Seven healthy males [means ± (SD): age 22.7±(6.8) yrs, height 178±(5.4) cm, weight 181.7±(16.9) lbs.] performed both a VR walking session and a non-VR walking session for 20 minutes on a treadmill at a speed of 80.4 m-minˉ¹. During each walking period the subjects wore surface EMG electrodes that were placed along the longitudinal axis of the vastus lateralis (VL) of the right thigh at a distance of 33.3% between the lateral femoral epicondyle and the greater trochanter. A repeated measures ANOVA was used to determine the differences in both EMG amplitude (RMS) and median frequency of firing (MDF). Results: The VR walking session resulted in significantly greater RMS values compared to the non-VR walking session (p \u3c 0.05); however there was no main effect for time or interaction between condition and time. For EMG MDF, there were no significant main effects for condition or time, and no significant interaction. Conclusion: The results indicate that walking during VR of the lower limbs may have a greater impact on neural adaptation of the VL due to increased motor unit activation as indicated by an increased RMS value. This might occur because blood flow restriction affects motor unit recruitment patterns resulting in the recruitment of more high-threshold, fast-twitch muscle fibers

Pathogen- and Host-Directed Antileishmanial Effects Mediated by Polyhexanide (PHMB)

BACKGROUND:Cutaneous leishmaniasis (CL) is a neglected tropical disease caused by protozoan parasites of the genus Leishmania. CL causes enormous suffering in many countries worldwide. There is no licensed vaccine against CL, and the chemotherapy options show limited efficacy and high toxicity. Localization of the parasites inside host cells is a barrier to most standard chemo- and immune-based interventions. Hence, novel drugs, which are safe, effective and readily accessible to third-world countries and/or drug delivery technologies for effective CL treatments are desperately needed. METHODOLOGY/PRINCIPAL FINDINGS:Here we evaluated the antileishmanial properties and delivery potential of polyhexamethylene biguanide (PHMB; polyhexanide), a widely used antimicrobial and wound antiseptic, in the Leishmania model. PHMB showed an inherent antileishmanial activity at submicromolar concentrations. Our data revealed that PHMB kills Leishmania major (L. major) via a dual mechanism involving disruption of membrane integrity and selective chromosome condensation and damage. PHMB's DNA binding and host cell entry properties were further exploited to improve the delivery and immunomodulatory activities of unmethylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODN). PHMB spontaneously bound CpG ODN, forming stable nanopolyplexes that enhanced uptake of CpG ODN, potentiated antimicrobial killing and reduced host cell toxicity of PHMB. CONCLUSIONS:Given its low cost and long history of safe topical use, PHMB holds promise as a drug for CL therapy and delivery vehicle for nucleic acid immunomodulators

The Political Economy of Natural Resource Use: Lessons for Fisheries Reform

This report discusses key lessons drawn from reform experience in the wider natural resource sector that might inform successful reform in fisheries. This report is a compilation of 12 papers prepared by acknowledged international experts in the fields of fisheries and wider natural resource reform which were reviewed at a workshop convened by the Property and Environment Research Center (PERC) in May 2009.The report forms an important initial input into an ongoing enquiry into the political economy of fisheries reform initiated by the World Bank in partnership with the Partnership for African Fisheries (a United Kingdom Department for International Development funded program of the New Partnership for African Development (NEPAD))

Cost of poor adherence to anti-hypertensive therapy in five European countries

The financial burden for EU health systems associated with cardiovascular disease (CV) has been estimated to be nearly €110 billion in 2006, corresponding to 10 % of total healthcare expenditure across EU or a mean €223 annual cost per capita. The main purpose of this study is to estimate the costs related to hypertension and the economic impact of increasing adherence to anti-hypertensive therapy in five European countries (Italy, Germany, France, Spain and England). A probabilistic prevalence-based decision tree model was developed to estimate the direct costs of CV related to hypertension (CV defined as: stroke, heart attack, heart failure) in five European countries. Our model considered adherence to hypertension treatment as a main driver of blood pressure (BP) control (BP < 140/90 mmHg). Relative risk of CV, based on controlled or uncontrolled BP group, was estimated from the Framingham Heart Study and national review data. Prevalence and cost data were estimated from national literature reviews. A national payer (NP) perspective for 10 years was considered. Probabilistic sensitivity analysis was performed in order to evaluate uncertainty around the results (given as 95 % confidence intervals). The model estimated a total of 8.6 million (1.4 in Italy, 3.3 in Germany, 1.2 in Spain, 1.8 in France and 0.9 in England) CV events related to hypertension over the 10-year time horizon. Increasing the adherence rate to anti-hypertensive therapy to 70 % (baseline value is different for each country) would lead to 82,235 fewer CV events (24,058 in Italy, 7,870 in Germany, 18,870 in Spain, 24,855 in France and 6,553 in England). From the NP perspective, the direct cost associated with hypertension was estimated to be €51.3 billion (8.1 in Italy, 17.1 in Germany, 12.2 in Spain, 8.8 in France and 5.0 in England). Increasing adherence to anti-hypertensive therapy to 70 % would save a total of €332 million (CI 95 %: €319-346 million) from the NPs perspective. This study is the first attempt to estimate the economic impact of non-adherence amongst patients with diagnosed hypertension in Europe, using data from five European countries (Italy, France, Germany, Spain and England). © 2014 Springer-Verlag Berlin Heidelberg

Fuzzy modelling of acid mine drainage environments using geochemical, ecological and mineralogical indicators

Fuzzy logic was applied to model acid mine drainage (AMD) and to obtain a classification index of the environmental impact in a contaminated riverine system. The data set used to develop this fuzzy model (a fuzzy classifier) concerns an abandoned mine in Northern Portugal— Valdarcas mining site. Here, distinctive drainage environments (spatial patterns) can be observed based on the AMD formed in the sulphide-rich waste-dumps. Such environments were established, as the effluent flows through the mining area, using several kinds of indicators. These are physical–chemical, ecological and mineralogical parameters, being expressed in a quantitative or qualitative basis. The fuzzy classifier proposed in this paper is a min– max fuzzy inference system, representing the spatial behaviour of those indicators, using the AMD environments as patterns. As they represent different levels (classes) of contamination, the fuzzy classifier can be used as a tool, allowing a more reasonable approach, compared with classical models, to characterize the environmental impact caused by AMD. In a general way it can be applied to other sites where sulphide-rich waste-dumps are promoting the pollution of superficial water through the generation of AMD

Attractiveness of a Four-component Pheromone Blend to Male Navel Orangeworm Moths

The attractiveness to male navel orangeworm moth, Amyelois transitella, of various combinations of a four-component pheromone blend was measured in wind-tunnel bioassays. Upwind flight along the pheromone plume and landing on the odor source required the simultaneous presence of two components, (11Z,13Z)-hexadecadienal and (3Z,6Z,9Z,12Z,15Z)-tricosapentaene, and the addition of either (11Z,13Z)-hexadecadien-1-ol or (11Z,13E)-hexadecadien-1-ol. A mixture of all four components produced the highest levels of rapid source location and source contact. In wind-tunnel assays, males did not seem to distinguish among a wide range of ratios of any of the three components added to (11Z,13Z)-hexadecadienal. Dosages of 10 and 100 ng of the 4-component blend produced higher levels of source location than dosages of 1 and 1,000 ng