An Oral Vaccine Based on U-Omp19 Induces Protection against B. abortus Mucosal Challenge by Inducing an Adaptive IL-17 Immune Response in Mice

As Brucella infections occur mainly through mucosal surfaces, the development of mucosal administered vaccines could be radical for the control of brucellosis. In this work we evaluated the potential of Brucella abortus 19 kDa outer membrane protein (U-Omp19) as an edible subunit vaccine against brucellosis. We investigated the protective immune response elicited against oral B. abortus infection after vaccination of mice with leaves from transgenic plants expressing U-Omp19; or with plant-made or E. coli-made purified U-Omp19. All tested U-Omp19 formulations induced protection against Brucella when orally administered without the need of adjuvants. U-Omp19 also induced protection against a systemic challenge when parenterally administered. This built-in adjuvant ability of U-Omp19 was independent of TLR4 and could be explained at least in part by its capability to activate dendritic cells in vivo. While unadjuvanted U-Omp19 intraperitoneally administered induced a specific Th1 response, following U-Omp19 oral delivery a mixed specific Th1-Th17 response was induced. Depletion of CD4+ T cells in mice orally vaccinated with U-Omp19 resulted in a loss of the elicited protection, indicating that this cell type mediates immune protection. The role of IL-17 against Brucella infection has never been explored. In this study, we determined that if IL-17A was neutralized in vivo during the challenge period, the mucosal U-Omp19 vaccine did not confer mucosal protection. On the contrary, IL-17A neutralization during the infection did not influence at all the subsistence and growth of this bacterium in PBS-immunized mice. All together, our results indicate that an oral unadjuvanted vaccine based on U-Omp19 induces protection against a mucosal challenge with Brucella abortus by inducing an adaptive IL-17 immune response. They also indicate different and important new aspects i) IL-17 does not contribute to reduce the bacterial burden in non vaccinated mice and ii) IL-17 plays a central role in vaccine mediated anti-Brucella mucosal immunity

Importance of identification and typing of Brucellae from West African cattle: a review

&lt;p&gt;Bovine brucellosis is an endemic infectious disease which can impact cattle productivity and welfare negatively, as well as human health. Sufficient knowledge on its epidemiology, particularly on species and biotypes of Brucella at national and/or regional scale are important to set up and implement efficient control measures against brucellosis in a &quot;One health&quot; perspective. The main objective of this review was to investigate available literature on strains of Brucella in order to provide a state of art-knowledge on species and biovars reported in cattle from West Africa. A literature search was conducted to identify relevant data on species and biovars of Brucella in cattle from Western African countries. This search included studies presenting bacteriological and/or molecular results of identification and typing, relied on international classification methods with no time limit and no language restrictions. Studies reporting results of identification at genus level only were not considered for this review. This review revealed that Brucella abortus was the most prevalent species in cattle from West Africa, in line with host preference for Brucellae. So far, biovars 1, 2, 3, 4, 6 and intermediate biovar 3/6 of B. abortus were reported in cattle in the region. Among these strains, biovars 3, recently identified in The Gambia and Ivory Coast, was the most commonly isolated. Brucella melitensis and/or B. suis have not been mentioned yet in cattle in this part of Africa. The public health significance of prevailing strains is discussed and a regional collaborative control program of brucellosis is suggested.&lt;/p&gt;</p