First observations of oblique ionospheric sounding chirp signal in Mexico

The results of the first experiment of oblique ionospheric sounding (OIS) chirp signal reception in Mexico are reported. Maximal and Lowest Observed Frequencies variations were studied under the quiet Space Weather conditions. The diurnal ionospheric variations by OIS signal confirm the results based on GNSS data in the Mexican region. The best HF radio propagation conditions along the considered path are during morning and daytime hours. The multi-hop propagation is frequent. The interlayer propagation modes are present at nighttime

Rhythmic beating of stem cell-derived cardiac cells requires dynamic coupling of electrophysiology and Ca cycling

There is an intense interest in differentiating embryonic stem cells to engineer biological pacemakers as an alternative to electronic pacemakers for patients with cardiac pacemaker function deficiency. Embryonic stem cell-derived cardiocytes (ESCs), however, often exhibit dysrhythmic excitations. Using Ca 2+ imaging and patch-clamp techniques, we studied requirements for generation of spontaneous rhythmic action potentials (APs) in late-stage mouse ESCs. Sarcoplasmic reticulum (SR) of ESCs generates spontaneous, rhythmic, wavelet-like Local Ca 2+ Releases (LCRs) (inhibited by ryanodine, tetracaine, or thapsigargin). L-type Ca 2+current (I CaL) induces a global Ca 2+ release (CICR), depleting the Ca 2+ content SR which resets the phases of LCR oscillators. Following a delay, SR then generates a highly synchronized spontaneous Ca 2+release of multiple LCRs throughout the cell. The LCRs generate an inward Na +/Ca 2+exchanger (NCX) current (absent in Na +-free solution) that ignites the next AP. Interfering with SR Ca 2+ cycling (ryanodine, caffeine, thapsigargin, cyclopiazonic acid, BAPTA-AM), NCX (Na +-free solution), or I CaL (nifedipine) results in dysrhythmic excitations or cessation of automaticity. Inhibition of cAMP/PKA signaling by a specific PKA inhibitor, PKI, decreases SR Ca 2+ loading, substantially reducing both spontaneous LCRs (number, size, and amplitude) and rhythmic AP firing. In contrast, enhancing PKA signaling by cAMP increases the LCRs (number, size, duration) and converts irregularly beating ESCs to rhythmic "pacemaker-like" cells. SR Ca 2+ loading and LCR activity could be also increased with a selective activation of SR Ca 2+ pumping by a phospholamban antibody. We conclude that SR Ca 2+ loading and spontaneous rhythmic LCRs are driven by inherent cAMP/PKA activity. I CaL synchronizes multiple LCR oscillators resulting in strong, partially synchronized diastolic Ca 2+ release and NCX current. Rhythmic ESC automaticity can be achieved by boosting "coupling" factors, such as cAMP/PKA signaling, that enhance interactions between SR and sarcolemma. © 2010.link_to_subscribed_fulltex

TEC behavior over the Mexican region

With the advent of the Navigation Satellites the Total Electron Content (TEC) has become one of the main parameters of the ionosphere. This is the result of a continuous TEC monitoring and rather dense network of GPS receivers. For Mexican region having no ionosondes the use of TEC for ionospheric conditions studies and monitoring has a special value. To study the behaviour of TEC and for its applied aspects two types of source-files are used worldwide: IONEX (global maps) and RINEX (local data) depending on the task solved. Magnetometer and satellite data from CHAMP and DMSP were involved in the analysis. First, benefits and limitations of TEC derived from both types of files are discussed in regard to the estimation of the ionosphere state in the Mexican region. Second, using both methods the specific features of diurnal, seasonal and annual patterns in TEC behaviour over Mexico were revealed, among which are the shift of the diurnal maximum to 14 LT, dependence on solar activity, high probability of night-time enhancements, presence of annual and winter anomalies. Third, it was revealed that the positive short-lived TEC enhancements are characteristic for Mexican region. They may occur even under quiet conditions. The answer is given what part of the ionosphere is responsible for TEC change during these positive disturbances. The results for Mexico were compared to the neighboring regions and South-East zone

MicroRNA-762 Is Upregulated in Human Corneal Epithelial Cells in Response to Tear Fluid and Pseudomonas aeruginosa Antigens and Negatively Regulates the Expression of Host Defense Genes Encoding RNase7 and ST2

Mucosal surfaces regulate defenses against infection and excessive inflammation. We previously showed that human tears upregulated epithelial expression of genes encoding RNase7 and ST2, which inhibited Pseudomonas aeruginosa invasion of human corneal epithelial cells. Here, microRNA microarrays were used to show that a combination of tear fluid exposure (16 h) then P. aeruginosa antigens (3 h) upregulated miR-762 and miR-1207, and down-regulated miR-92 and let-7b (all > 2-fold) in human corneal epithelial cells compared to P. aeruginosa antigens alone. RT-PCR confirmed miR-762 upregulation ∼ 3-fold in tear-antigen exposed cells. Without tears or antigens, an antagomir reduced miR-762 expression relative to scrambled controls by ∼50%, increased expression of genes encoding RNase7 (∼80 %), ST2 (∼58%) and Rab5a (∼75%), without affecting P. aeruginosa internalization. However, P. aeruginosa invasion was increased > 3-fold by a miR-762 mimic which reduced RNase7 and ST2 gene expression. Tear fluid alone also induced miR-762 expression ∼ 4-fold, which was reduced by the miR-762 antagomir. Combination of tear fluid and miR-762 antagomir increased RNase7 and ST2 gene expression. These data show that mucosal fluids, such as tears, can modulate epithelial microRNA expression to regulate innate defense genes, and that miR-762 negatively regulates RNase7, ST2 and Rab5a genes. Since RNase7 and ST2 inhibit P. aeruginosa internalization, and are upregulated by tear fluid, other tear-induced mechanisms must counteract inhibitory effects of miR-762 to regulate resistance to bacteria. These data also suggest a complex relationship between tear induction of miR-762, its modulation of innate defense genes, and P. aeruginosa internalization