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Efeito do nĂvel de urĂ©ia na dieta sobre o desempenho, a qualidade e o estĂĄdio de desenvolvimento embrionĂĄrio em cabras Alpinas
Erratum To: Effects Of Partial Inhibition Of Respiratory Complex I On H2o2 Production By Isolated Brain Mitochondria In Different Respiratory States
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Effects Of Partial Inhibition Of Respiratory Complex I On H2o2 Production By Isolated Brain Mitochondria In Different Respiratory States
The aim of this work was to characterize the effects of partial inhibition of respiratory complex I by rotenone on H2O2 production by isolated rat brain mitochondria in different respiratory states. Flow cytometric analysis of membrane potential in isolated mitochondria indicated that rotenone leads to uniform respiratory inhibition when added to a suspension of mitochondria. When mitochondria were incubated in the presence of a low concentration of rotenone (10 nm) and NADH-linked substrates, oxygen consumption was reduced from 45.9 ± 1.0 to 26.4 ± 2.6 nmol O2 mgâ1 minâ1 and from 7.8 ± 0.3 to 6.3 ± 0.3 nmol O2 mgâ1 minâ1 in respiratory states 3 (ADP-stimulated respiration) and 4 (resting respiration), respectively. Under these conditions, mitochondrial H2O2 production was stimulated from 12.2 ± 1.1 to 21.0 ± 1.2 pmol H2O2 mgâ1 minâ1 and 56.5 ± 4.7 to 95.0 ± 11.1 pmol H2O2 mgâ1 minâ1 in respiratory states 3 and 4, respectively. Similar results were observed when comparing mitochondrial preparations enriched with synaptic or nonsynaptic mitochondria or when 1-methyl-4-phenylpyridinium ion (MPP+) was used as a respiratory complex I inhibitor. Rotenone-stimulated H2O2 production in respiratory states 3 and 4 was associated with a high reduction state of endogenous nicotinamide nucleotides. In succinate-supported mitochondrial respiration, where most of the mitochondrial H2O2 production relies on electron backflow from complex II to complex I, low rotenone concentrations inhibited H2O2 production. Rotenone had no effect on mitochondrial elimination of micromolar concentrations of H2O2. 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Viable offspring after successful non-surgical embryo transfer in goats
O objetivo do presente estudo foi avaliar a viabilidade da tĂ©cnica de transferĂȘncia nĂŁo cirĂșrgica em cabras. Quatro cabras nĂŁo-lactantes plurĂparas da raça Toggenburg foram utilizadas como receptoras de embriĂ”es, sendo que duas receberam um embriĂ”es e duas receberam dois embriĂ”es coletados nĂŁo cirurgicamente cabras doadoras. Os corpos lĂșteos das receptoras foram detectados um dia antes da transferĂȘncia de embriĂ”es por ultrassonografia transretal. Uma seringa de 5mL contendo 2mL de meio holding foi acoplada em um cateter tomcat, no qual os embriĂ”es foram aspirados em uma coluna central a duas outras colunas. Um espĂ©culo Colin nĂșmero 2 foi inserido na vulva e na vagina, e com o uso de uma fonte de luz, a cerviz foi localizada e imobilizada com uma pinça de Allis. Um cateter uretral nĂșmero seis acoplado a um mandril e lubrificado com meio PBS foi inserido na cĂ©rvix, e assim os aneis cervicais foram gradualmente transpostos. ApĂłs perder a resistĂȘncia, o cateter uretral foi movido lateralmente para o corno uterino desejado. O mandril e a pinça de Allis foram retirados e o conjunto seringa e tomcat foi acoplado ao cateter uretral e o conteĂșdo injetado no corno uterino ipsilateral ao corpo lĂșteo com posterior retirada do cateter. Cabras que ovularam em apenas um ovĂĄrio foram usadas para testar a eficiĂȘncia da deposição do embriĂŁo. O tempo gasto entre a inserção do espĂ©culo e a sua remoção foi inferior a trĂȘs minutos. O tempo para transpor a cĂ©rvix foi inferior a um minuto. A ultrassonografia revelou a deposição de lĂquido no corno desejado. Receptoras que receberam dois embriĂ”es tornaram-se gestantes e pariram trĂȘs crias. Estes primeiros resultados encorajam a tĂ©cnica e demonstram que a transferĂȘncia de embriĂ”es em caprinos pode ser feita totalmente por procedimentos nĂŁo cirĂșrgicos
Effect of breed and corpus luteum on pregnancy rate of bovine embryo recipients
The objective of this study was to evaluate pregnancy rates of recipients of different breed groups (Nellore and crossbreed), as well as the effects of size and type of the corpus luteum (CL) on plasmatic concentrations of progesterone and pregnancy rates of embryo recipients. A total of 152 heifers were synchronized with progesterone implants and on the day of embryo transfer, previously obtained by superovulation and frozen in ethylene glycol, the diameter and type of the corpus luteum (cavitary and compact) was measured and blood was collected for progesterone measurement. The pregnancy rate was 44.1%, with a diameter of corpus luteum higher in recipients that became pregnant (2.03±0.41) compared with non-pregnant ones (1.86±0.34 cm). Plasmatic concentrations of progesterone did not differ between pregnant (1.50±1.05) and non-pregnant (1.31±0.91 ng/mL) animals. The type of corpus luteum did not influence the pregnancy rates. Only Angus and crossbred Marchigiana differ among themselves in pregnancy rates (33.3 and 59.2%, respectively). The pregnancy probability was affected only by CL diameter, but not by P4 plasmatic concentration. Selection of the corpus luteum size at the time of embryo transfer is an important factor to increase pregnancy rates in recipients, and compact and cavitary corpora lutea do not influence the pregnancy rates of bovine embryo recipients. Nellore recipients have pregnancy rates that are satisfactory and comparable to crossbred (Bos taurus à Bos indicus) recipients