Maine Distributed Solar Valuation Study

During its 2014 session, the Maine Legislature enacted an Act to Support Solar Energy Development in Maine. P.L Chapter 562 (April 24, 2014) (codified at 35‐A M.R.S. §§ 3471‐3473) (“Act”). Section 1 of the Act contains the Legislative finding that it is in the public interest is to develop renewable energy resources, including solar energy, in a manner that protects and improves the health and well‐being of the citizens and natural environment of the State while also providing economic benefits to communities, ratepayers and the overall economy of the State. Section 2 of the Act requires the Public Utilities Commission (Commission) to determine the value of distributed solar energy generation in the State, evaluate implementation options, and to deliver a report to the Legislature. To support this work, the Commission engaged a project team comprising Clean Power Research (Napa, California), Sustainable Energy Advantage (Framingham, Massachusetts), Pace Energy and Climate Center at the Pace Law School (White Plains, New York), and Dr. Richard Perez (Albany, New York). Under the project, the team developed the methodology under a Commission‐run stakeholder review process, conducted a valuation on distributed solar for three utility territories, and developed a summary of implementation options for increasing deployment of distributed solar generation in the State. The report includes three volumes which accompany this Executive Summary: Volume I Methodology; Volume II Valuation Results; Volume III Implementation Options

Text Entry in Immersive Head-Mounted Display-Based Virtual Reality Using Standard Keyboards

We study the performance and user experience of two popular mainstream text entry devices, desktop keyboards and touchscreen keyboards, for use in Virtual Reality (VR) applications. We discuss the limitations arising from limited visual feedback, and examine the efficiency of different strategies of use. We analyze a total of 24 hours of typing data in VR from 24 participants and find that novice users are able to retain about 60% of their typing speed on a desktop keyboard and about 40-45\% of their typing speed on a touchscreen keyboard. We also find no significant learning effects, indicating that users can transfer their typing skills fast into VR. Besides investigating baseline performances, we study the position in which keyboards and hands are rendered in space. We find that this does not adversely affect performance for desktop keyboard typing and results in a performance trade-off for touchscreen keyboard typing

Using molecular dynamics simulations as an aid in the prediction of domain swapping of computationally designed protein variants

In standard implementations of computational protein design, a positive-design approach is used to predict sequences that will be stable on a given backbone structure. Possible competing states are typically not considered, primarily because appropriate structural models are not available. One potential competing state, the domain-swapped dimer, is especially compelling because it is often nearly identical to its monomeric counterpart, differing by just a few mutations in a hinge region. Molecular dynamics (MD) simulations provide a computational method to sample different conformational states of a structure. Here, we tested whether MD simulations could be used as a post-design screening tool to identify sequence mutations leading to domain-swapped dimers. We hypothesized that a successful computationally-designed sequence would have backbone structure and dynamics characteristics similar to that of the input structure, and that in contrast, domain-swapped dimers would exhibit increased backbone flexibility and/or altered structure in the hinge-loop region to accommodate the large conformational change required for domain swapping. While attempting to engineer a homodimer from a 51 amino acid fragment of the monomeric protein engrailed homeodomain (ENH), we had instead generated a domain-swapped dimer (ENH_DsD). MD simulations on these proteins showed increased MD simulation derived B factors in the hinge loop of the ENH_DsD domain-swapped dimer relative to monomeric ENH. Two point mutants of ENH_DsD designed to recover the monomeric fold were then tested with an MD simulation protocol. The MD simulations suggested that one of these mutants would adopt the target monomeric structure, which was subsequently confirmed by X-ray crystallography

Mixed Spices at Culinary Doses Have Prebiotic Effects in Healthy Adults: A Pilot Study.

Spices were used as food preservatives prior to the advent of refrigeration, suggesting the possibility of effects on microbiota. Previous studies have shown prebiotic activities in animals and in vitro, but there has not been a demonstration of prebiotic or postbiotic effects at culinary doses in humans. In this randomized placebo-controlled study, we determined in twenty-nine healthy adults the effects on the gut microbiota of the consumption daily of capsules containing 5 g of mixed spices at culinary doses by comparison to a matched control group consuming a maltodextrin placebo capsule. The 16S ribosomal RNA sequencing data were used for microbial characterization. Spice consumption resulted in a significant reduction in Firmicutes abundance (p < 0.033) and a trend of enrichment in Bacteroidetes (p < 0.097) compared to placebo group. Twenty-six operational taxonomic units (OTUs) were different between the spice and placebo groups after intervention. Furthermore, there was a significant negative correlation between fecal short-chain fatty acid propionate concentration and Firmicutes abundance in spice intervention group (p < 0.04). The production of individual fecal short-chain fatty acid was not significantly changed by spice consumption in this study. Mixed spices consumption significantly modified gut microbiota, suggesting a prebiotic effect of spice consumption at culinary doses

Sonic hedgehog medulloblastoma cancer stem cells mirnome and transcriptome highlight novel functional networks

Molecular classification has improved the knowledge of medulloblastoma (MB), the most common malignant brain tumour in children, however current treatments cause severe side effects in patients. Cancer stem cells (CSCs) have been described in MB and represent a sub population characterised by self-renewal and the ability to generate tumour cells, thus representing the reservoir of the tumour. To investigate molecular pathways that characterise this sub population, we isolated CSCs from Sonic Hedgehog Medulloblastoma (SHH MB) arisen in Patched 1 (Ptch1) heterozygous mice, and performed miRNA-and mRNA-sequencing. Comparison of the miRNA-sequencing of SHH MB CSCs with that obtained from cerebellar Neural Stem Cells (NSCs), allowed us to obtain a SHH MB CSC miRNA differential signature. Pathway enrichment analysis in SHH MB CSCs mirnome and transcriptome was performed and revealed a series of enriched pathways. We focused on the putative targets of the SHH MB CSC miRNAs that were involved in the enriched pathways of interest, namely pathways in cancer, PI3k-Akt pathway and protein processing in endoplasmic reticulum pathway. In silico analysis was performed in SHH MB patients and identified several genes, whose expression was associated with worse overall survival of SHH MB patients. This study provides novel candidates whose functional role should be further investigated in SHH MB

In vitro effects of fungicides on the fungus Haliphthoros philippinensis

Pure cultures of Haliphthoros philippinensis, isolated from infected Penaeus monodon larvae, were exposed for 24 hours to varying concentrations of antifungal agents. The efficiency of each agent to inhibit sporulation and mycelial growth was measured. Effects on P. monodon eggs and larvae were also investigated. It is concluded that preliminary bioassay of larval tolerance to the suggested effective doses should always be made prior to prophylaxix or therapeutic applications

In-vitro effect of fungicides on hyphal growth and sporogenesis of Lagenidium sp. isolated from Penaeus monodon larvae and Scylla serrata eggs

The sensitivity of Lagenidium, isolated from Penaeus monodon, Scylla serrata , to 34 antimycotics was determined. Effects on the development of vesicles, zoospores and mycelial growth were evaluated. Although mycoidal levels of the chemicals tested will be ideal for lethal treatment on control of the fungus, the high dose required may be lethal to the host, thus the use of mycostatic concentrations is more practical. Treatments of rearing water containing larvae, adult shrimps or crabs should be done only after preliminary tolerance experiments using at least the mycostatic dose prove to be safe for the hosts. Mycocidal doses can be used for determining disinfection doses of equipment and facilities used in rearing procedures as well as for destroying batches of infected larvae

Evaluation of Selected Aspects of the Nutrition Therapeutic Programme Offered to HIV-Positive Women of Child-Bearing Age in Western Cape Province, South Africa

Background: The Nutrition Therapeutic Programme (NTP) involves the provision of food supplements at primary health clinics (PHCs) to correct nutritional deficiencies in vulnerable groups. Although previous studies have identified problems with implementing the programme at PHCs, assessments of its efficiency have been scarce.Objective: To evaluate implementation of the NTP at PHCs that provide antiretroviral therapy.Methods: A cross-sectional, descriptive study was conducted at 17 PHCs located within 3 districts of Western Cape Province. Two target groups were chosen: 32 staff members working at the sites and 21 women of child-bearing age enrolled in the NTP. Questionnaires were used to obtain data.Results: Only 2 women (10%) lived in food-secure households; the rest were either at risk of hunger (29%) or classified as hungry (61%). Most of the women knew they had to take the supplements to improve their nutritional status, but the majority only recalled receiving basic nutritional advice, and the information was mainly given verbally. Ten of the women had shared their supplements with others, mostly with their children. The study identified lack of clearly defined NTP responsibilities at the PHCs, causing confusion amongst the staff. Although many staff members expressed problems with the NTP, only 38% of them reported having routine evaluations regarding the programme.Conclusion: Several aspects compromised the effectiveness of the NTP, including socioeconomic factors leading to clients’ non-compliance. The strategic organisation and implementation of the NTP varied between different PHCs offering antiretroviral therapy, and staff experienced difficulties with the logistics of the programme

Toponyms for centers of endemism in Madagascar

A biogeographical model was proposed in 2006 to explain the centers of endemism and the importance of riparian forest of some watersheds as refuges or dispersal corridors during paleoclimatic oscillations. Here, we consider these geographical features highlighting their biological and socio-cultural importance. We explain the etymology or eponymy of the major rivers of the retreat - dispersal watersheds, i.e., the drainage basins of Bemarivo, Antainambalana, Mangoro, Manampatrana, Mananara South, Mandrare, Onilahy, Mangoky, Tsiribihina, Betsiboka, Maevarano, Sambirano, and Mahavavy North. We propose a toponymy for each of the 15 centers of endemism and highlight their peculiarities. We named the centers of endemism of Vohimarina, Masoala, Analanjirofo, Tanala, Manombo, Anosy, Ranopiso, Karimbola, Mikea, Menabe, Melaky, Sofia, Ampasindava, Ankify, and Ankarana. We illustrate each center of endemism with a flagship species and report on its natural and cultural histories, and conservation.RÉSUMÉUn modèle biogéographique a été proposé en 2006 pour expliquer les centres d’endémisme de la biodiversité et l’importance des ripisylves de certains bassins versants en tant que refuges ou couloirs de dispersion au cours des oscillations paléoclimatiques. Ici, nous considérons ces dispositifs géographiques en soulignant leur importance biologique et socio-culturelle. Dans un premier temps, nous expliquons la toponymie ou l’éponymie des grands fleuves des bassins refuges et de dispersion, à savoir les bassins de la Bemarivo, de l’Antainambalana, du Mangoro, de la Manampatrana, de la Mananara du Sud, du Mandrare, de l’Onilahy, du Mangoky, de la Tsiribihina, de la Betsiboka, de la Maevarano, du Sambirano et de la Mahavavy du Nord. Puis nous proposons une toponymie pour chacun des 17 centres et sous-centres d’endémisme en justifiant leurs particularités. Nous retenons ainsi les centres d’endémisme de Vohimarina, de l’Atsinanana (dont Masoala et Analanjirofo), Tanala, de Manombo, de l’Anosy, d’Ala maika (dont Ranopiso, Karimbola et Mikea), du Menabe, du Melaky, de la Sofia, d’Ampasindava, d’Ankify et de l’Ankarana. Nous illustrons chacun des centres d’endémisme avec une espèce symbolique et rapportons des aspects de son histoire naturelle et culturelle ainsi que de sa conservation